Restriction map for
pFN21AA0189
(Circular) (Six-base) MAPSORT of: pFN21AA0189.seq Check: 381 from: 1 to: 7774
pFN21AA0189 7774 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5584 278
Size: 53 83 186 936 4048 2468
Fragments arranged by size:
4048 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5117 1937
Size: 3180 4594
Acc65I G'GTAC_C
Cuts at: 5095 5095
Size: 7774
AclI AA'CG_TT
Cuts at: 1563 5901 6274 1563
Size: 4338 373 3063
Fragments arranged by size:
4338 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2904 3159 5837 7221 7489 1632
Size: 1272 255 2678 1384 268 1917
Fragments arranged by size:
2678 1917 1384 1272 268 255
AfeI AGC'GCT
Cuts at: 4505 4505
Size: 7774
AflII C'TTAA_G
Cuts at: 820 1017 4108 820
Size: 197 3091 4486
Fragments arranged by size:
4486 3091 197
AflIII A'CryG_T
Cuts at: 1203 2946 6673 7650 1203
Size: 1743 3727 977 1327
Fragments arranged by size:
3727 1743 1327 977
AgeI A'CCGG_T
Cuts at: 6605 6605
Size: 7774
AhdI GACnn_n'nnGTC
Cuts at: 6503 6503
Size: 7774
AleI CACnn'nnGTG
Cuts at: 1166 2646 4607 5001 1166
Size: 1480 1961 394 3939
Fragments arranged by size:
3939 1961 1480 394
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7774
AlwNI CAG_nnn'CTG
Cuts at: 3474 3757 3805 3859 4096 4300 4320 4483 4693
Size: 283 48 54 237 204 20 163 210
Cuts at: 4693 4834 5517 7089 3474
Size: 141 683 1572 4159
Fragments arranged by size:
4159 1572 683 283 237 210 204 163
141 54 48 20
ApaI G_GGCC'C
Cuts at: 3133 4536 4660 4687 5060 3133
Size: 1403 124 27 373 5847
Fragments arranged by size:
5847 1403 373 124 27
ApaLI G'TGCA_C
Cuts at: 2660 5831 6987 2660
Size: 3171 1156 3447
Fragments arranged by size:
3447 3171 1156
ApoI r'AATT_y
Cuts at: 1493 5083 5309 5461 7570 1493
Size: 3590 226 152 2109 1697
Fragments arranged by size:
3590 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6328 160
Size: 872 5296 1606
Fragments arranged by size:
5296 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7774
AvaI C'yCGr_G
Cuts at: 1870 1943 2264 2305 2982 5099 1870
Size: 73 321 41 677 2117 4545
Fragments arranged by size:
4545 2117 677 321 73 41
AvrII C'CTAG_G
Cuts at: 4262 4262
Size: 7774
BamHI G'GATC_C
Cuts at: 3849 4837 5104 5471 3849
Size: 988 267 367 6152
Fragments arranged by size:
6152 988 367 267
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2576 2784 3718 4171 4339
Size: 325 217 627 789 208 934 453 168
Cuts at: 4339 4564 4575 4747 5095 6550 618
Size: 225 11 172 348 1455 1842
Fragments arranged by size:
1842 1455 934 789 627 453 348 325
225 217 208 172 168 11
BanII G_rGCy'C
Cuts at: 1391 2177 2451 2795 3133 3590 3966 4392 4536
Size: 786 274 344 338 457 376 426 144
Cuts at: 4536 4660 4687 4876 5060 5093 1391
Size: 124 27 189 184 33 4072
Fragments arranged by size:
4072 786 457 426 376 344 338 274
189 184 144 124 33 27
BbeI G_GCGC'C
Cuts at: 2788 4175 2788
Size: 1387 6387
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2073 928
Size: 971 174 6629
Fragments arranged by size:
6629 971 174
BbvCI CC'TCA_GC
Cuts at: 2324 2560 2877 3078 3180 3210 3387 2324
Size: 236 317 201 102 30 177 6711
Fragments arranged by size:
6711 317 236 201 177 102 30
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2207 2241 5965 5999 1903
Size: 34 270 34 3724 34 3678
Fragments arranged by size:
3724 3678 270 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5666 6876 5666
Size: 1210 6564
BclI T'GATC_A
Cuts at: 1548 4311 1548
Size: 2763 5011
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2600 2764 4248
Size: 107 122 71 631 605 928 164 1484
Cuts at: 4248 4401 6385 136
Size: 153 1984 1525
Fragments arranged by size:
1984 1525 1484 928 631 605 164 153
122 107 71
BglII A'GATC_T
Cuts at: 4002 7769 4002
Size: 3767 4007
BlpI GC'TnA_GC
Cuts at: 2856 4386 4391 4416 4500 5191 2856
Size: 1530 5 25 84 691 5439
Fragments arranged by size:
5439 1530 691 84 25 5
Bme1580I G_kGCm'C
Cuts at: 1790 2299 2664 3133 3633 4344 4536 4578 4660
Size: 509 365 469 500 711 192 42 82
Cuts at: 4660 4687 5060 5835 6991 1790
Size: 27 373 775 1156 2573
Fragments arranged by size:
2573 1156 775 711 509 500 469 373
365 192 82 42 27
BmgBI CAC'GTC
Cuts at: 1318 3922 1318
Size: 2604 5170
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2116 2557 4822
Size: 416 53 83 399 24 699 441 2265
Cuts at: 4822 4885 5505 6463 442
Size: 63 620 958 1753
Fragments arranged by size:
2265 1753 958 699 620 441 416 399
83 63 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7774
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 7774
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2924 3030 3159 3331 3398 3560 3983
Size: 818 867 106 129 172 67 162 423
Cuts at: 3983 6434 1239
Size: 2451 2579
Fragments arranged by size:
2579 2451 867 818 423 172 162 129
106 67
Bpu10I CC'TnA_GC
Cuts at: 2324 2560 2841 2877 3078 3168 3180 3210 3387
Size: 236 281 36 201 90 12 30 177
Cuts at: 3387 3960 4451 2324
Size: 573 491 5647
Fragments arranged by size:
5647 573 491 281 236 201 177 90
36 30 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2179 2378 5899 6764 7062 2179
Size: 199 3521 865 298 2891
Fragments arranged by size:
3521 2891 865 298 199
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2142 2485 2985 3869 6437 882
Size: 617 643 343 500 884 2568 2219
Fragments arranged by size:
2568 2219 884 643 617 500 343
BsaAI yAC'GTr
Cuts at: 493 1204 2826 2947 3709 3736 5565 493
Size: 711 1622 121 762 27 1829 2702
Fragments arranged by size:
2702 1829 1622 762 711 121 27
BsaBI GATnn'nnATC
Cuts at: 4671 7768 4671
Size: 3097 4677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2785 4172 5581 5963
Size: 53 83 186 936 1252 1387 1409 382
Cuts at: 5963 6598 275
Size: 635 1451
Fragments arranged by size:
1451 1409 1387 1252 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4567 4840 6207 6605 6879 7026 7437 4567
Size: 273 1367 398 274 147 411 4904
Fragments arranged by size:
4904 1367 411 398 274 273 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7549
Fragments arranged by size:
7549 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2312 2915 3038 3041 3334 3688 4004 1186
Size: 1126 603 123 3 293 354 316 4956
Fragments arranged by size:
4956 1126 603 354 316 293 123 3
BseYI C'CCAG_C
Cuts at: 2300 2851 3121 3223 3241 3518 3645 4405 4536
Size: 551 270 102 18 277 127 760 131
Cuts at: 4536 4621 6977 7473 2300
Size: 85 2356 496 2601
Fragments arranged by size:
2601 2356 760 551 496 277 270 131
127 102 85 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3563 3758 4387 1163
Size: 573 1827 195 629 4550
Fragments arranged by size:
4550 1827 629 573 195
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5224 5985 7013 1540
Size: 275 186 3223 761 1028 2301
Fragments arranged by size:
3223 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2664 4392 5835 5920 6991 2664
Size: 1728 1443 85 1071 3447
Fragments arranged by size:
3447 1728 1443 1071 85
BsmI GAATG_Cn'
Cuts at: 3768 5285 5378 3768
Size: 1517 93 6164
Fragments arranged by size:
6164 1517 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 2299 2451 2664 2795 3133 3590
Size: 399 387 122 152 213 131 338 457
Cuts at: 3590 3633 3966 4344 4392 4536 4578 4660 4687
Size: 43 333 378 48 144 42 82 27
Cuts at: 4687 4876 5060 5093 5835 5920 6991 1391
Size: 189 184 33 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 457 399 387 378 338
333 213 189 184 152 144 131 122
85 82 48 43 42 33 27
BspEI T'CCGG_A
Cuts at: 4840 7437 4840
Size: 2597 5177
BspHI T'CATG_A
Cuts at: 3669 5663 7393 3669
Size: 1994 1730 4050
Fragments arranged by size:
4050 1994 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3867 3939 3963 4491 4806 5129 844
Size: 3023 72 24 528 315 323 3489
Fragments arranged by size:
3489 3023 528 323 315 72 24
BsrBI CCG'CTC
Cuts at: 1394 1823 4654 4923 5661 1394
Size: 429 2831 269 738 3507
Fragments arranged by size:
3507 2831 738 429 269
BsrDI GCAATG_nn'
Cuts at: 65 1248 4116 6269 6443 65
Size: 1183 2868 2153 174 1396
Fragments arranged by size:
2868 2153 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2313 2564 3549 6418 6605 1700
Size: 110 503 251 985 2869 187 2869
Fragments arranged by size:
2869 2869 985 503 251 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7774
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7774
BssSI C'ACGA_G
Cuts at: 4803 5834 6846 4803
Size: 1031 1012 5731
Fragments arranged by size:
5731 1031 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3781 3801 4582 794
Size: 2987 20 781 3986
Fragments arranged by size:
3986 2987 781 20
BstBI TT'CG_AA
Cuts at: 6578 6578
Size: 7774
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7774
BstXI CCAn_nnnn'nTGG
Cuts at: 4609 5014 4609
Size: 405 7369
BstYI r'GATC_y
Cuts at: 1972 3849 4002 4672 4837 5022 5104 5471 5856
Size: 1877 153 670 165 185 82 367 385
Cuts at: 5856 5873 7325 7411 7423 7769 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1877 1452 670 385 367 346 185
165 153 86 82 17 12
Bsu36I CC'TnA_GG
Cuts at: 2727 4955 2727
Size: 2228 5546
BtgI C'CryG_G
Cuts at: 513 1065 3109 5007 513
Size: 552 2044 1898 3280
Fragments arranged by size:
3280 2044 1898 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7035
Fragments arranged by size:
7035 683 56
BtsI GCAGTG_nn'
Cuts at: 2093 2578 3590 3755 4167 5290 6084 6104 2093
Size: 485 1012 165 412 1123 794 20 3763
Fragments arranged by size:
3763 1123 1012 794 485 412 165 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7774
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7739
DraI TTT'AAA
Cuts at: 5078 5078
Size: 7774
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6781 809
Size: 507 5465 1802
Fragments arranged by size:
5465 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5221 6110 7739
Size: 54 1425 50 275 18 3391 889 1629
Cuts at: 7739 8
Size: 43
Fragments arranged by size:
3391 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5221 1537
Size: 275 3409 4090
Fragments arranged by size:
4090 3409 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2070 2076 3015 3198 4461 5704 1353
Size: 717 6 939 183 1263 1243 3423
Fragments arranged by size:
3423 1263 1243 939 717 183 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2431 6361 6735 6881 387
Size: 1064 138 842 3930 374 146 1280
Fragments arranged by size:
3930 1280 1064 842 374 146 138
EcoICRI GAG'CTC
Cuts at: 4390 4390
Size: 7774
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2904 2924 3030 3159 3331 3398
Size: 393 425 847 20 106 129 172 67
Cuts at: 3398 3560 3983 5837 6434 7221 7489 1239
Size: 162 423 1854 597 787 268 1524
Fragments arranged by size:
1854 1524 847 787 597 425 423 393
268 172 162 129 106 67 20
EcoNI CCTnn'n_nnAGG
Cuts at: 3581 4019 4219 3581
Size: 438 200 7136
Fragments arranged by size:
7136 438 200
EcoO109I rG'GnC_Cy
Cuts at: 2148 2348 3005 3214 3399 3456 3952 4259 4267
Size: 200 657 209 185 57 496 307 8
Cuts at: 4267 4297 4480 4532 4533 4657 5057 2148
Size: 30 183 52 1 124 400 4865
Fragments arranged by size:
4865 657 496 400 307 209 200 185
183 124 57 52 30 8 1
EcoRI G'AATT_C
Cuts at: 5083 5461 5083
Size: 378 7396
FspI TGC'GCA
Cuts at: 3794 6280 3794
Size: 2486 5288
HaeII r_GCGC'y
Cuts at: 1678 2374 2788 4175 4507 4792 6921 1678
Size: 696 414 1387 332 285 2129 2531
Fragments arranged by size:
2531 2129 1387 696 414 332 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2707 2739 2963 2995 3893
Size: 32 135 32 1135 32 224 32 898
Cuts at: 3893 3925 4106 4138 4199 4231 6415 6447 6489
Size: 32 181 32 61 32 2184 32 42
Cuts at: 6489 6521 1373
Size: 32 2626
Fragments arranged by size:
2626 2184 1135 898 224 181 135 61
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5118 5364 669
Size: 988 281 3180 246 3079
Fragments arranged by size:
3180 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7774
HpaI GTT'AAC
Cuts at: 5364 5364
Size: 7774
KasI G'GCGC_C
Cuts at: 2784 4171 2784
Size: 1387 6387
KpnI G_GTAC'C
Cuts at: 5099 5099
Size: 7774
MfeI C'AATT_G
Cuts at: 5373 5373
Size: 7774
MluI A'CGCG_T
Cuts at: 7650 7650
Size: 7774
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6888 7072 1305
Size: 5583 184 2007
Fragments arranged by size:
5583 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5952
Fragments arranged by size:
5952 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2249 2646 3734 4191 4607 5001 5012
Size: 648 1083 397 1088 457 416 394 11
Cuts at: 5012 5732 6091 6250 518
Size: 720 359 159 2042
Fragments arranged by size:
2042 1088 1083 720 648 457 416 397
394 359 159 11
MspA1I CmG'CkG
Cuts at: 1606 2525 2855 3179 4363 4385 4625 5054 5190
Size: 919 330 324 1184 22 240 429 136
Cuts at: 5190 5867 7015 7260 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1184 1148 919 677 429 330 324
245 240 136 22
NarI GG'CG_CC
Cuts at: 2785 4172 2785
Size: 1387 6387
NcoI C'CATG_G
Cuts at: 513 1065 3109 5007 513
Size: 552 2044 1898 3280
Fragments arranged by size:
3280 2044 1898 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7774
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7774
NotI GC'GGCC_GC
Cuts at: 5221 5221
Size: 7774
NspI r_CATG'y
Cuts at: 1132 1229 5132 6677 1132
Size: 97 3903 1545 2229
Fragments arranged by size:
3903 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2335 4294 1348
Size: 987 1959 4828
Fragments arranged by size:
4828 1959 987
PciI A'CATG_T
Cuts at: 6673 6673
Size: 7774
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2547 3805 4351 4693 4834 1108
Size: 1439 1258 546 342 141 4048
Fragments arranged by size:
4048 1439 1258 546 342 141
PfoI T'CCnGG_A
Cuts at: 3845 3948 4476 4647 5025 3845
Size: 103 528 171 378 6594
Fragments arranged by size:
6594 528 378 171 103
PmeI GTTT'AAAC
Cuts at: 5078 5078
Size: 7774
PmlI CAC'GTG
Cuts at: 2947 3736 2947
Size: 789 6985
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5843 5875 7356 7388 5843
Size: 32 1481 32 6229
Fragments arranged by size:
6229 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2148 2348 3399 3952 4267 4480 2148
Size: 200 1051 553 315 213 5442
Fragments arranged by size:
5442 1051 553 315 213 200
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7774
PsiI TTA'TAA
Cuts at: 5344 5344
Size: 7774
PspOMI G'GGCC_C
Cuts at: 3129 4532 4656 4683 5056 3129
Size: 1403 124 27 373 5847
Fragments arranged by size:
5847 1403 373 124 27
PspXI vC'TCGA_Gb
Cuts at: 2305 2305
Size: 7774
PstI C_TGCA'G
Cuts at: 830 3479 4362 4441 5049 5126 830
Size: 2649 883 79 608 77 3478
Fragments arranged by size:
3478 2649 883 608 79 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7774
PvuII CAG'CTG
Cuts at: 2525 2855 3179 4363 4385 4625 2525
Size: 330 324 1184 22 240 5674
Fragments arranged by size:
5674 1184 330 324 240 22
SacI G_AGCT'C
Cuts at: 4392 4392
Size: 7774
SalI G'TCGA_C
Cuts at: 1936 5116 1936
Size: 3180 4594
SanDI GG'GwC_CC
Cuts at: 2348 3399 2348
Size: 1051 6723
SapI GCTCTTCn'nnn_
Cuts at: 2076 2076
Size: 7774
SbfI CC_TGCA'GG
Cuts at: 3479 5049 5126 3479
Size: 1570 77 6127
Fragments arranged by size:
6127 1570 77
ScaI AGT'ACT
Cuts at: 6022 7734 6022
Size: 1712 6062
SfcI C'TryA_G
Cuts at: 826 1045 2957 3475 4088 4358 4375 4437 5045
Size: 219 1912 518 613 270 17 62 608
Cuts at: 5045 5122 6257 6938 7129 826
Size: 77 1135 681 191 1471
Fragments arranged by size:
1912 1471 1135 681 613 608 518 270
219 191 77 62 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 2600 2600
Size: 7774
SfoI GGC'GCC
Cuts at: 2786 4173 2786
Size: 1387 6387
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7774
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7774
SmaI CCC'GGG
Cuts at: 1872 5101 1872
Size: 3229 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2194 2305 2393 4108 5878 6779
Size: 197 926 251 111 88 1715 1770 901
Cuts at: 6779 7041 820
Size: 262 1553
Fragments arranged by size:
1770 1715 1553 926 901 262 251 197
111 88
SnaBI TAC'GTA
Cuts at: 493 5565 493
Size: 5072 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7774
SphI G_CATG'C
Cuts at: 1132 5132 1132
Size: 4000 3774
SspI AAT'ATT
Cuts at: 5 52 5698 5
Size: 47 5646 2081
Fragments arranged by size:
5646 2081 47
StuI AGG'CCT
Cuts at: 1517 2594 2603 1517
Size: 1077 9 6688
Fragments arranged by size:
6688 1077 9
StyI C'CwwG_G
Cuts at: 513 1065 3109 4020 4262 5007 5213 513
Size: 552 2044 911 242 745 206 3074
Fragments arranged by size:
3074 2044 911 745 552 242 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2348 4307 5801 5818 5971 6156 6601
Size: 1255 768 1959 1494 17 153 185 445
Cuts at: 6601 325
Size: 1498
Fragments arranged by size:
1959 1498 1494 1255 768 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3621 6020 7732
Size: 274 80 33 51 1443 1644 2399 1712
Cuts at: 7732 96
Size: 138
Fragments arranged by size:
2399 1712 1644 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2072 2104 2114 2146 3714 3746 7734 7766 2072
Size: 32 10 32 1568 32 3988 32 2080
Fragments arranged by size:
3988 2080 1568 32 32 32 32 10
Tth111I GACn'n_nGTC
Cuts at: 1316 3890 1316
Size: 2574 5200
XbaI T'CTAG_A
Cuts at: 5110 5110
Size: 7774
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2766 2766
Size: 7774
XhoI C'TCGA_G
Cuts at: 1943 2305 1943
Size: 362 7412
XmaI C'CCGG_G
Cuts at: 1870 5099 1870
Size: 3229 4545
XmnI GAAnn'nnTTC
Cuts at: 4331 5903 4331
Size: 1572 6202
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5582 276
Size: 53 83 186 936 4048 2468
Fragments arranged by size:
4048 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I
DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PsrI RsrII SacII SexAI SrfI SwaI