(Circular) (Six-base) MAPSORT of: pFN21AA0160.seq Check: 1 from: 1 to: 6922 pFN21AA0160 6922 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2629 2629 Size: 6922 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4732 278 Size: 53 83 186 936 3196 2468 Fragments arranged by size: 3196 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4265 1937 Size: 2328 4594 Acc65I G'GTAC_C Cuts at: 4243 4243 Size: 6922 AclI AA'CG_TT Cuts at: 1563 5049 5422 1563 Size: 3486 373 3063 Fragments arranged by size: 3486 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3351 3663 4131 4985 6369 6637 1632 Size: 1719 312 468 854 1384 268 1917 Fragments arranged by size: 1917 1719 1384 854 468 312 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6725 AflIII A'CryG_T Cuts at: 1203 2355 5821 6798 1203 Size: 1152 3466 977 1327 Fragments arranged by size: 3466 1327 1152 977 AgeI A'CCGG_T Cuts at: 2212 5753 2212 Size: 3541 3381 AhdI GACnn_n'nnGTC Cuts at: 5651 5651 Size: 6922 AleI CACnn'nnGTG Cuts at: 1166 2025 1166 Size: 859 6063 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6922 AlwNI CAG_nnn'CTG Cuts at: 4665 6237 4665 Size: 1572 5350 ApaI G_GGCC'C Cuts at: 2052 2052 Size: 6922 ApaLI G'TGCA_C Cuts at: 4979 6135 4979 Size: 1156 5766 ApoI r'AATT_y Cuts at: 1493 2542 2680 2711 3145 3629 3955 4231 4457 Size: 1049 138 31 434 484 326 276 226 Cuts at: 4457 4609 6718 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1049 484 434 326 276 226 152 138 31 AseI AT'TA_AT Cuts at: 160 1032 2802 5476 160 Size: 872 1770 2674 1606 Fragments arranged by size: 2674 1770 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6922 AvaI C'yCGr_G Cuts at: 1870 1943 2044 4247 1870 Size: 73 101 2203 4545 Fragments arranged by size: 4545 2203 101 73 BamHI G'GATC_C Cuts at: 3766 4252 4619 3766 Size: 486 367 6069 Fragments arranged by size: 6069 486 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2641 4243 5698 618 Size: 325 217 627 221 633 1602 1455 1842 Fragments arranged by size: 1842 1602 1455 633 627 325 221 217 BanII G_rGCy'C Cuts at: 1391 2052 4241 1391 Size: 661 2189 4072 Fragments arranged by size: 4072 2189 661 BbeI G_GCGC'C Cuts at: 2012 2012 Size: 6922 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5951 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5113 5147 1903 Size: 34 3176 34 3678 Fragments arranged by size: 3678 3176 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3247 4814 6024 3247 Size: 1567 1210 4145 Fragments arranged by size: 4145 1567 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6922 BfrBI ATG'CAT Cuts at: 3994 3994 Size: 6922 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5533 136 Size: 107 122 71 631 605 3861 1525 Fragments arranged by size: 3861 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2289 3229 6917 2289 Size: 940 3688 2294 Fragments arranged by size: 3688 2294 940 BlpI GC'TnA_GC Cuts at: 4339 4339 Size: 6922 Bme1580I G_kGCm'C Cuts at: 1790 2052 4983 6139 1790 Size: 262 2931 1156 2573 Fragments arranged by size: 2931 2573 1156 262 BmgBI CAC'GTC Cuts at: 1318 2239 1318 Size: 921 6001 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4653 5611 442 Size: 416 53 83 399 24 3236 958 1753 Fragments arranged by size: 3236 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6922 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3051 3051 Size: 6922 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 2356 5582 1239 Size: 1010 107 3226 2579 Fragments arranged by size: 3226 2579 1010 107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3380 5047 5912 6210 3380 Size: 1667 865 298 4092 Fragments arranged by size: 4092 1667 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3089 5585 882 Size: 617 1590 2496 2219 Fragments arranged by size: 2496 2219 1590 617 BsaAI yAC'GTr Cuts at: 493 1204 4713 493 Size: 711 3509 2702 Fragments arranged by size: 3509 2702 711 BsaBI GATnn'nnATC Cuts at: 3522 6916 3522 Size: 3394 3528 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 4729 5111 5746 Size: 53 83 186 936 476 2720 382 635 Cuts at: 5746 275 Size: 1451 Fragments arranged by size: 2720 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2212 5355 5753 6027 6174 6585 2212 Size: 3143 398 274 147 411 2549 Fragments arranged by size: 3143 2549 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2150 2180 1178 Size: 30 165 30 747 30 5920 Fragments arranged by size: 5920 747 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2158 2161 2164 2167 1186 Size: 972 3 3 3 5941 Fragments arranged by size: 5941 972 3 3 3 BseYI C'CCAG_C Cuts at: 2051 6125 6621 2051 Size: 4074 496 2352 Fragments arranged by size: 4074 2352 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3616 1163 Size: 573 1880 4469 Fragments arranged by size: 4469 1880 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4372 5133 6161 1540 Size: 275 186 2371 761 1028 2301 Fragments arranged by size: 2371 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2238 4983 5068 6139 2238 Size: 2745 85 1071 3021 Fragments arranged by size: 3021 2745 1071 85 BsmI GAATG_Cn' Cuts at: 4433 4526 4433 Size: 93 6829 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2238 4241 4983 5068 6139 1391 Size: 399 262 186 2003 742 85 1071 2174 Fragments arranged by size: 2174 2003 1071 742 399 262 186 85 BspEI T'CCGG_A Cuts at: 6585 6585 Size: 6922 BspHI T'CATG_A Cuts at: 3867 4811 6541 3867 Size: 944 1730 4248 Fragments arranged by size: 4248 1730 944 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2629 3354 3722 4277 844 Size: 1785 725 368 555 3489 Fragments arranged by size: 3489 1785 725 555 368 BsrBI CCG'CTC Cuts at: 1394 1823 4809 1394 Size: 429 2986 3507 Fragments arranged by size: 3507 2986 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3349 5417 5591 65 Size: 1183 2101 2068 174 1396 Fragments arranged by size: 2101 2068 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2212 5566 5753 1700 Size: 110 402 3354 187 2869 Fragments arranged by size: 3354 2869 402 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6922 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6922 BssSI C'ACGA_G Cuts at: 2252 4982 5994 2252 Size: 2730 1012 3180 Fragments arranged by size: 3180 2730 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6922 BstBI TT'CG_AA Cuts at: 2305 3042 5726 2305 Size: 737 2684 3501 Fragments arranged by size: 3501 2684 737 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6922 BstYI r'GATC_y Cuts at: 1972 2289 3229 3766 4252 4619 5004 5021 6473 Size: 317 940 537 486 367 385 17 1452 Cuts at: 6473 6559 6571 6917 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 940 537 486 385 367 346 317 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3513 3513 Size: 6922 BtgI C'CryG_G Cuts at: 513 1065 2004 2181 513 Size: 552 939 177 5254 Fragments arranged by size: 5254 939 552 177 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3509 487 Size: 683 56 2283 3900 Fragments arranged by size: 3900 2283 683 56 BtsI GCAGTG_nn' Cuts at: 4438 5232 5252 4438 Size: 794 20 6108 Fragments arranged by size: 6108 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6922 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6887 DraI TTT'AAA Cuts at: 2401 4226 2401 Size: 1825 5097 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5929 809 Size: 507 4613 1802 Fragments arranged by size: 4613 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3682 4369 5258 Size: 54 1425 50 275 18 1852 687 889 Cuts at: 5258 6887 8 Size: 1629 43 Fragments arranged by size: 1852 1629 1425 889 687 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4369 1537 Size: 275 2557 4090 Fragments arranged by size: 4090 2557 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2776 2832 3020 3084 3801 4852 1353 Size: 1423 56 188 64 717 1051 3423 Fragments arranged by size: 3423 1423 1051 717 188 64 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5509 5883 6029 387 Size: 1064 138 3920 374 146 1280 Fragments arranged by size: 3920 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2356 3351 3663 4131 4985 5582 Size: 393 617 107 995 312 468 854 597 Cuts at: 5582 6369 6637 1239 Size: 787 268 1524 Fragments arranged by size: 1524 995 854 787 617 597 468 393 312 268 107 EcoO109I rG'GnC_Cy Cuts at: 2048 3059 2048 Size: 1011 5911 EcoRI G'AATT_C Cuts at: 4231 4609 4231 Size: 378 6544 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2582 2582 Size: 6922 FspI TGC'GCA Cuts at: 5428 5428 Size: 6922 HaeII r_GCGC'y Cuts at: 1678 2012 2926 6069 1678 Size: 334 914 3143 2531 Fragments arranged by size: 3143 2531 914 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2402 2434 2435 2467 2501 Size: 32 135 32 830 32 1 32 34 Cuts at: 2501 2533 3145 3177 3214 3246 4169 4201 5563 Size: 32 612 32 37 32 923 32 1362 Cuts at: 5563 5595 5637 5669 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1362 923 830 612 135 42 37 34 32 32 32 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 4266 4512 669 Size: 988 281 2328 246 3079 Fragments arranged by size: 3079 2328 988 281 246 HindIII A'AGCT_T Cuts at: 748 4164 748 Size: 3416 3506 HpaI GTT'AAC Cuts at: 4512 4512 Size: 6922 KasI G'GCGC_C Cuts at: 2008 2008 Size: 6922 KpnI G_GTAC'C Cuts at: 4247 4247 Size: 6922 MfeI C'AATT_G Cuts at: 4521 4521 Size: 6922 MluI A'CGCG_T Cuts at: 6798 6798 Size: 6922 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3065 6036 6220 1305 Size: 1760 2971 184 2007 Fragments arranged by size: 2971 2007 1760 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3684 10 Size: 54 1425 343 1852 3248 Fragments arranged by size: 3248 1852 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2025 2505 4880 5239 5398 518 Size: 648 859 480 2375 359 159 2042 Fragments arranged by size: 2375 2042 859 648 480 359 159 MspA1I CmG'CkG Cuts at: 1606 2183 2192 4338 5015 6163 6408 1606 Size: 577 9 2146 677 1148 245 2120 Fragments arranged by size: 2146 2120 1148 677 577 245 9 NarI GG'CG_CC Cuts at: 2009 2009 Size: 6922 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5431 Fragments arranged by size: 5431 939 552 NdeI CA'TA_TG Cuts at: 387 2733 387 Size: 2346 4576 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6922 NotI GC'GGCC_GC Cuts at: 4369 4369 Size: 6922 NsiI A_TGCA'T Cuts at: 3996 3996 Size: 6922 NspI r_CATG'y Cuts at: 1132 1229 2359 3625 3994 4280 5825 1132 Size: 97 1130 1266 369 286 1545 2229 Fragments arranged by size: 2229 1545 1266 1130 369 286 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6922 PciI A'CATG_T Cuts at: 2355 5821 2355 Size: 3466 3456 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6922 PfoI T'CCnGG_A Cuts at: 2773 2773 Size: 6922 PmeI GTTT'AAAC Cuts at: 4226 4226 Size: 6922 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4991 5023 6504 6536 4991 Size: 32 1481 32 5377 Fragments arranged by size: 5377 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3059 3059 Size: 6922 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6922 PsiI TTA'TAA Cuts at: 4492 4492 Size: 6922 PspOMI G'GGCC_C Cuts at: 2048 2048 Size: 6922 PstI C_TGCA'G Cuts at: 830 4274 830 Size: 3444 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6922 SacII CC_GC'GG Cuts at: 2184 2184 Size: 6922 SalI G'TCGA_C Cuts at: 1936 4264 1936 Size: 2328 4594 SapI GCTCTTCn'nnn_ Cuts at: 2832 2832 Size: 6922 SbfI CC_TGCA'GG Cuts at: 4274 4274 Size: 6922 ScaI AGT'ACT Cuts at: 5170 6882 5170 Size: 1712 5210 SfcI C'TryA_G Cuts at: 826 1045 2293 3225 4270 5405 6086 6277 826 Size: 219 1248 932 1045 1135 681 191 1471 Fragments arranged by size: 1471 1248 1135 1045 932 681 219 191 SfoI GGC'GCC Cuts at: 2010 2010 Size: 6922 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6922 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6922 SmaI CCC'GGG Cuts at: 1872 4249 1872 Size: 2377 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3395 5026 5927 6189 820 Size: 197 926 1452 1631 901 262 1553 Fragments arranged by size: 1631 1553 1452 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4713 493 Size: 4220 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6922 SphI G_CATG'C Cuts at: 1132 3994 4280 1132 Size: 2862 286 3774 Fragments arranged by size: 3774 2862 286 SspI AAT'ATT Cuts at: 5 52 2331 2824 3288 4846 5 Size: 47 2279 493 464 1558 2081 Fragments arranged by size: 2279 2081 1558 493 464 47 StuI AGG'CCT Cuts at: 1517 2270 1517 Size: 753 6169 StyI C'CwwG_G Cuts at: 513 1065 2004 3356 4361 513 Size: 552 939 1352 1005 3074 Fragments arranged by size: 3074 1352 1005 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4949 4966 5119 5304 5749 325 Size: 1255 3369 17 153 185 445 1498 Fragments arranged by size: 3369 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3362 5168 6880 Size: 274 80 33 51 1443 1385 1806 1712 Cuts at: 6880 96 Size: 138 Fragments arranged by size: 1806 1712 1443 1385 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6882 6914 6882 Size: 32 6890 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6922 XbaI T'CTAG_A Cuts at: 4258 4258 Size: 6922 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2965 2965 Size: 6922 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6922 XmaI C'CCGG_G Cuts at: 1870 4247 1870 Size: 2377 4545 XmnI GAAnn'nnTTC Cuts at: 2841 5051 2841 Size: 2210 4712 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4730 276 Size: 53 83 186 936 3196 2468 Fragments arranged by size: 3196 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI