Restriction map for
pFN21AA0160
(Linear) (Six-base) MAPSORT of: KIAA0160.seq Check: 8851 from: 1 to: 2217
KIAA0160.orf
With 182 enzymes: SgfI *
July 23, 2007 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 624 2217
Size: 624 1593
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1346 1658 2126 2217
Size: 1346 312 468 91
Fragments arranged by size:
1346 468 312 91
AflIII A'CryG_T
Cuts at: 0 350 2217
Size: 350 1867
AgeI A'CCGG_T
Cuts at: 0 207 2217
Size: 207 2010
AleI CACnn'nnGTG
Cuts at: 0 20 2217
Size: 20 2197
ApaI G_GGCC'C
Cuts at: 0 47 2217
Size: 47 2170
ApoI r'AATT_y
Cuts at: 0 537 675 706 1140 1624 1950 2217
Size: 537 138 31 434 484 326 267
Fragments arranged by size:
537 484 434 326 267 138 31
AseI AT'TA_AT
Cuts at: 0 797 2217
Size: 797 1420
AvaI C'yCGr_G
Cuts at: 0 39 2217
Size: 39 2178
BamHI G'GATC_C
Cuts at: 0 1761 2217
Size: 1761 456
BanI G'GyrC_C
Cuts at: 0 3 636 2217
Size: 3 633 1581
Fragments arranged by size:
1581 633 3
BanII G_rGCy'C
Cuts at: 0 47 2217
Size: 47 2170
BbeI G_GCGC'C
Cuts at: 0 7 2217
Size: 7 2210
BciVI GTATCCnnnnn_n'
Cuts at: 0 1242 2217
Size: 1242 975
BfrBI ATG'CAT
Cuts at: 0 1989 2217
Size: 1989 228
BglII A'GATC_T
Cuts at: 0 284 1224 2217
Size: 284 940 993
Fragments arranged by size:
993 940 284
Bme1580I G_kGCm'C
Cuts at: 0 47 2217
Size: 47 2170
BmgBI CAC'GTC
Cuts at: 0 234 2217
Size: 234 1983
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1046 2217
Size: 1046 1171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 351 2217
Size: 244 107 1866
Fragments arranged by size:
1866 244 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1375 2217
Size: 1375 842
BsaI GGTCTCn'nnnn_
Cuts at: 0 1084 2217
Size: 1084 1133
BsaBI GATnn'nnATC
Cuts at: 0 1517 2217
Size: 1517 700
BsaHI Gr'CG_yC
Cuts at: 0 4 2217
Size: 4 2213
BsaWI w'CCGG_w
Cuts at: 0 207 2217
Size: 207 2010
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 145 175 2217
Size: 145 30 2042
Fragments arranged by size:
2042 145 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 153 156 159 162 2217
Size: 153 3 3 3 2055
Fragments arranged by size:
2055 153 3 3 3
BseYI C'CCAG_C
Cuts at: 0 46 2217
Size: 46 2171
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1611 2217
Size: 1611 606
BsiHKAI G_wGCw'C
Cuts at: 0 233 2217
Size: 233 1984
Bsp1286I G_dGCh'C
Cuts at: 0 47 233 2217
Size: 47 186 1984
Fragments arranged by size:
1984 186 47
BspHI T'CATG_A
Cuts at: 0 1862 2217
Size: 1862 355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 624 1349 1717 2217
Size: 624 725 368 500
Fragments arranged by size:
725 624 500 368
BsrDI GCAATG_nn'
Cuts at: 0 1344 2217
Size: 1344 873
BsrFI r'CCGG_y
Cuts at: 0 207 2217
Size: 207 2010
BssSI C'ACGA_G
Cuts at: 0 247 2217
Size: 247 1970
BstBI TT'CG_AA
Cuts at: 0 300 1037 2217
Size: 300 737 1180
Fragments arranged by size:
1180 737 300
BstYI r'GATC_y
Cuts at: 0 284 1224 1761 2217
Size: 284 940 537 456
Fragments arranged by size:
940 537 456 284
Bsu36I CC'TnA_GG
Cuts at: 0 1508 2217
Size: 1508 709
BtgI C'CryG_G
Cuts at: 0 176 2217
Size: 176 2041
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1504 2217
Size: 1504 713
DraI TTT'AAA
Cuts at: 0 396 2217
Size: 396 1821
EaeI y'GGCC_r
Cuts at: 0 1677 2217
Size: 1677 540
EarI CTCTTCn'nnn_
Cuts at: 0 771 827 1015 1079 1796 2217
Size: 771 56 188 64 717 421
Fragments arranged by size:
771 717 421 188 64 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 351 1346 1658 2126 2217
Size: 244 107 995 312 468 91
Fragments arranged by size:
995 468 312 244 107 91
EcoO109I rG'GnC_Cy
Cuts at: 0 43 1054 2217
Size: 43 1011 1163
Fragments arranged by size:
1163 1011 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 577 2217
Size: 577 1640
HaeII r_GCGC'y
Cuts at: 0 7 921 2217
Size: 7 914 1296
Fragments arranged by size:
1296 914 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 430 462 496 528 1140 1172
Size: 397 32 1 32 34 32 612 32
Cuts at: 1172 1209 1241 2164 2196 2217
Size: 37 32 923 32 21
Fragments arranged by size:
923 612 397 37 34 32 32 32
32 32 32 21 1
HindIII A'AGCT_T
Cuts at: 0 2159 2217
Size: 2159 58
KasI G'GCGC_C
Cuts at: 0 3 2217
Size: 3 2214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1060 2217
Size: 1060 1157
MscI TGG'CCA
Cuts at: 0 1679 2217
Size: 1679 538
MslI CAynn'nnrTG
Cuts at: 0 20 500 2217
Size: 20 480 1717
Fragments arranged by size:
1717 480 20
MspA1I CmG'CkG
Cuts at: 0 178 187 2217
Size: 178 9 2030
Fragments arranged by size:
2030 178 9
NarI GG'CG_CC
Cuts at: 0 4 2217
Size: 4 2213
NdeI CA'TA_TG
Cuts at: 0 728 2217
Size: 728 1489
NsiI A_TGCA'T
Cuts at: 0 1991 2217
Size: 1991 226
NspI r_CATG'y
Cuts at: 0 354 1620 1989 2217
Size: 354 1266 369 228
Fragments arranged by size:
1266 369 354 228
PciI A'CATG_T
Cuts at: 0 350 2217
Size: 350 1867
PfoI T'CCnGG_A
Cuts at: 0 768 2217
Size: 768 1449
PpuMI rG'GwC_Cy
Cuts at: 0 1054 2217
Size: 1054 1163
PspOMI G'GGCC_C
Cuts at: 0 43 2217
Size: 43 2174
SacII CC_GC'GG
Cuts at: 0 179 2217
Size: 179 2038
SapI GCTCTTCn'nnn_
Cuts at: 0 827 2217
Size: 827 1390
SfcI C'TryA_G
Cuts at: 0 288 1220 2217
Size: 288 932 997
Fragments arranged by size:
997 932 288
SfoI GGC'GCC
Cuts at: 0 5 2217
Size: 5 2212
SmlI C'TyrA_G
Cuts at: 0 1390 2217
Size: 1390 827
SphI G_CATG'C
Cuts at: 0 1989 2217
Size: 1989 228
SspI AAT'ATT
Cuts at: 0 326 819 1283 2217
Size: 326 493 464 934
Fragments arranged by size:
934 493 464 326
StuI AGG'CCT
Cuts at: 0 265 2217
Size: 265 1952
StyI C'CwwG_G
Cuts at: 0 1351 2217
Size: 1351 866
TatI w'GTAC_w
Cuts at: 0 1357 2217
Size: 1357 860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 960 2217
Size: 960 1257
XmnI GAAnn'nnTTC
Cuts at: 0 836 2217
Size: 836 1381
Enzymes that do cut:
AarI AcuI AflIII AgeI AleI ApaI ApoI AseI
AvaI BamHI BanI BanII BbeI BciVI BfrBI BglII
Bme1580I BmgBI BplI BpmI BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrDI BsrFI BssSI BstBI BstYI Bsu36I BtgI
BtgZI DraI EaeI EarI Eco57MI EcoO109I FalI HaeII
Hin4I HindIII KasI MmeI MscI MslI MspA1I NarI
NdeI NsiI NspI PciI PfoI PpuMI PspOMI SacII
SapI SfcI SfoI SmlI SphI SspI StuI StyI
TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AhdI AlfI
AloI AlwNI ApaLI AscI AsiSI AvrII BaeI BbsI
BbvCI BcgI BclI BglI BlpI BmrI BmtI Bpu10I
BsaAI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrGI
BssHII BstAPI BstEII BstXI BstZ17I BtsI ClaI CspCI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
NaeI NcoI NgoMIV NheI NotI NruI PacI PasI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SwaI TaqII TstI Tth111I XbaI XhoI XmaI
ZraI