(Circular) (Six-base) MAPSORT of: pFN21AA0038.seq Check: 1935 from: 1 to: 5389 pFN21AA0038 5389 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3199 278 Size: 53 83 186 936 1663 2468 Fragments arranged by size: 2468 1663 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2732 1937 Size: 795 4594 Acc65I G'GTAC_C Cuts at: 2710 2710 Size: 5389 AclI AA'CG_TT Cuts at: 1563 3516 3889 1563 Size: 1953 373 3063 Fragments arranged by size: 3063 1953 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2401 2479 3452 4836 5104 1632 Size: 769 78 973 1384 268 1917 Fragments arranged by size: 1917 1384 973 769 268 78 AflII C'TTAA_G Cuts at: 820 1017 2288 820 Size: 197 1271 3921 Fragments arranged by size: 3921 1271 197 AflIII A'CryG_T Cuts at: 1203 4288 5265 1203 Size: 3085 977 1327 Fragments arranged by size: 3085 1327 977 AgeI A'CCGG_T Cuts at: 4220 4220 Size: 5389 AhdI GACnn_n'nnGTC Cuts at: 4118 4118 Size: 5389 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5389 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5389 AlwNI CAG_nnn'CTG Cuts at: 3132 4704 3132 Size: 1572 3817 ApaLI G'TGCA_C Cuts at: 2313 3446 4602 2313 Size: 1133 1156 3100 Fragments arranged by size: 3100 1156 1133 ApoI r'AATT_y Cuts at: 1493 2243 2267 2698 2924 3076 5185 1493 Size: 750 24 431 226 152 2109 1697 Fragments arranged by size: 2109 1697 750 431 226 152 24 AseI AT'TA_AT Cuts at: 160 1032 3943 160 Size: 872 2911 1606 Fragments arranged by size: 2911 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5389 AvaI C'yCGr_G Cuts at: 1870 1943 2714 1870 Size: 73 771 4545 Fragments arranged by size: 4545 771 73 BamHI G'GATC_C Cuts at: 2519 2719 3086 2519 Size: 200 367 4822 Fragments arranged by size: 4822 367 200 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2648 2710 4165 618 Size: 325 217 627 861 62 1455 1842 Fragments arranged by size: 1842 1455 861 627 325 217 62 BanII G_rGCy'C Cuts at: 1391 2124 2708 1391 Size: 733 584 4072 Fragments arranged by size: 4072 733 584 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4418 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2162 2196 3580 3614 1903 Size: 34 225 34 1384 34 3678 Fragments arranged by size: 3678 1384 225 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3281 4491 3281 Size: 1210 4179 BclI T'GATC_A Cuts at: 1548 1548 Size: 5389 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4000 136 Size: 107 122 71 631 605 2328 1525 Fragments arranged by size: 2328 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5384 5384 Size: 5389 BlpI GC'TnA_GC Cuts at: 2806 2806 Size: 5389 Bme1580I G_kGCm'C Cuts at: 1790 2317 3450 4606 1790 Size: 527 1133 1156 2573 Fragments arranged by size: 2573 1156 1133 527 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5389 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3120 4078 442 Size: 416 53 83 399 24 1703 958 1753 Fragments arranged by size: 1753 1703 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5389 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2561 4049 1239 Size: 1322 1488 2579 Fragments arranged by size: 2579 1488 1322 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3514 4379 4677 3514 Size: 865 298 4226 Fragments arranged by size: 4226 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2560 4052 882 Size: 617 1061 1492 2219 Fragments arranged by size: 2219 1492 1061 617 BsaAI yAC'GTr Cuts at: 493 1204 2140 3180 493 Size: 711 936 1040 2702 Fragments arranged by size: 2702 1040 936 711 BsaBI GATnn'nnATC Cuts at: 5383 5383 Size: 5389 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2603 3196 3578 4213 Size: 53 83 186 936 1070 593 382 635 Cuts at: 4213 275 Size: 1451 Fragments arranged by size: 1451 1070 936 635 593 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3822 4220 4494 4641 5052 3822 Size: 398 274 147 411 4159 Fragments arranged by size: 4159 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2559 2589 1178 Size: 30 165 30 1156 30 3978 Fragments arranged by size: 3978 1156 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5389 BseYI C'CCAG_C Cuts at: 4592 5088 4592 Size: 496 4893 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4816 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2032 2329 2464 2839 3600 4628 Size: 275 186 31 297 135 375 761 1028 Cuts at: 4628 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 375 297 275 186 135 31 BsiHKAI G_wGCw'C Cuts at: 2317 2563 3450 3535 4606 2317 Size: 246 887 85 1071 3100 Fragments arranged by size: 3100 1071 887 246 85 BsmI GAATG_Cn' Cuts at: 2900 2993 2900 Size: 93 5296 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2317 2563 2708 3450 3535 4606 Size: 399 334 193 246 145 742 85 1071 Cuts at: 4606 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 334 246 193 145 85 BspEI T'CCGG_A Cuts at: 5052 5052 Size: 5389 BspHI T'CATG_A Cuts at: 3278 5008 3278 Size: 1730 3659 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2744 844 Size: 1900 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3276 1394 Size: 429 1453 3507 Fragments arranged by size: 3507 1453 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2346 3884 4058 65 Size: 1183 1098 1538 174 1396 Fragments arranged by size: 1538 1396 1183 1098 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2463 4033 4220 1700 Size: 110 653 1570 187 2869 Fragments arranged by size: 2869 1570 653 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5389 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5389 BssSI C'ACGA_G Cuts at: 3449 4461 3449 Size: 1012 4377 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5389 BstBI TT'CG_AA Cuts at: 4193 4193 Size: 5389 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5389 BstYI r'GATC_y Cuts at: 1972 2194 2519 2719 3086 3471 3488 4940 5026 Size: 222 325 200 367 385 17 1452 86 Cuts at: 5026 5038 5384 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 325 222 200 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2068 2086 2481 2515 513 Size: 552 939 64 18 395 34 3387 Fragments arranged by size: 3387 939 552 395 64 34 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4650 Fragments arranged by size: 4650 683 56 BtsI GCAGTG_nn' Cuts at: 2080 2905 3699 3719 2080 Size: 825 794 20 3750 Fragments arranged by size: 3750 825 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5389 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5354 DraI TTT'AAA Cuts at: 2248 2693 2248 Size: 445 4944 DraIII CAC_nnn'GTG Cuts at: 2339 2339 Size: 5389 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4396 809 Size: 507 3080 1802 Fragments arranged by size: 3080 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 2836 3725 Size: 54 1425 50 275 18 253 753 889 Cuts at: 3725 5354 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 753 275 253 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2836 1537 Size: 275 1024 4090 Fragments arranged by size: 4090 1024 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2544 3319 1353 Size: 1191 775 3423 Fragments arranged by size: 3423 1191 775 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 3976 4350 4496 387 Size: 1064 138 435 1952 374 146 1280 Fragments arranged by size: 1952 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2401 2479 2561 3452 4049 4836 5104 Size: 393 769 78 82 891 597 787 268 Cuts at: 5104 1239 Size: 1524 Fragments arranged by size: 1524 891 787 769 597 393 268 82 78 EcoO109I rG'GnC_Cy Cuts at: 2064 2474 2064 Size: 410 4979 EcoRI G'AATT_C Cuts at: 2267 2698 3076 2267 Size: 431 378 4580 Fragments arranged by size: 4580 431 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2314 2314 Size: 5389 FspI TGC'GCA Cuts at: 3895 3895 Size: 5389 HaeII r_GCGC'y Cuts at: 1678 4536 1678 Size: 2858 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2559 2591 2652 2684 4030 Size: 32 135 32 987 32 61 32 1346 Cuts at: 4030 4062 4104 4136 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1346 987 135 61 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2733 2979 669 Size: 988 281 795 246 3079 Fragments arranged by size: 3079 988 795 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5389 HpaI GTT'AAC Cuts at: 2979 2979 Size: 5389 KpnI G_GTAC'C Cuts at: 2714 2714 Size: 5389 MfeI C'AATT_G Cuts at: 2988 2988 Size: 5389 MluI A'CGCG_T Cuts at: 5265 5265 Size: 5389 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2547 4503 4687 1305 Size: 1242 1956 184 2007 Fragments arranged by size: 2007 1956 1242 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2085 10 Size: 54 1425 343 253 3314 Fragments arranged by size: 3314 1425 343 253 54 MslI CAynn'nnrTG Cuts at: 518 1166 3347 3706 3865 518 Size: 648 2181 359 159 2042 Fragments arranged by size: 2181 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2070 2805 3482 4630 4875 1606 Size: 464 735 677 1148 245 2120 Fragments arranged by size: 2120 1148 735 677 464 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2086 2481 513 Size: 552 939 82 395 3421 Fragments arranged by size: 3421 939 552 395 82 NdeI CA'TA_TG Cuts at: 387 387 Size: 5389 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5389 NotI GC'GGCC_GC Cuts at: 2836 2836 Size: 5389 NruI TCG'CGA Cuts at: 2600 2600 Size: 5389 NspI r_CATG'y Cuts at: 1132 1229 2747 4292 1132 Size: 97 1518 1545 2229 Fragments arranged by size: 2229 1545 1518 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5389 PciI A'CATG_T Cuts at: 4288 4288 Size: 5389 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5389 PmeI GTTT'AAAC Cuts at: 2693 2693 Size: 5389 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3458 3490 4971 5003 3458 Size: 32 1481 32 3844 Fragments arranged by size: 3844 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2064 2064 Size: 5389 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5389 PsiI TTA'TAA Cuts at: 2959 2959 Size: 5389 PstI C_TGCA'G Cuts at: 830 2741 830 Size: 1911 3478 PvuI CG_AT'CG Cuts at: 2001 2032 2329 2001 Size: 31 297 5061 Fragments arranged by size: 5061 297 31 SacII CC_GC'GG Cuts at: 2071 2071 Size: 5389 SalI G'TCGA_C Cuts at: 1936 2731 1936 Size: 795 4594 SanDI GG'GwC_CC Cuts at: 2064 2064 Size: 5389 SbfI CC_TGCA'GG Cuts at: 2741 2741 Size: 5389 ScaI AGT'ACT Cuts at: 3637 5349 3637 Size: 1712 3677 SfcI C'TryA_G Cuts at: 826 1045 2038 2737 3872 4553 4744 826 Size: 219 993 699 1135 681 191 1471 Fragments arranged by size: 1471 1135 993 699 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5389 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5389 SmaI CCC'GGG Cuts at: 1872 2716 1872 Size: 844 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2288 3493 4394 4656 820 Size: 197 926 345 1205 901 262 1553 Fragments arranged by size: 1553 1205 926 901 345 262 197 SnaBI TAC'GTA Cuts at: 493 2140 3180 493 Size: 1647 1040 2702 Fragments arranged by size: 2702 1647 1040 SpeI A'CTAG_T Cuts at: 152 152 Size: 5389 SphI G_CATG'C Cuts at: 1132 2747 1132 Size: 1615 3774 SspI AAT'ATT Cuts at: 5 52 3313 5 Size: 47 3261 2081 Fragments arranged by size: 3261 2081 47 StuI AGG'CCT Cuts at: 1517 2656 1517 Size: 1139 4250 StyI C'CwwG_G Cuts at: 513 1065 2004 2086 2481 2828 513 Size: 552 939 82 395 347 3074 Fragments arranged by size: 3074 939 552 395 347 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2319 3416 3433 3586 3771 4216 325 Size: 1255 739 1097 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1097 739 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3635 5347 96 Size: 274 80 33 51 1443 1658 1712 138 Fragments arranged by size: 1712 1658 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5349 5381 5349 Size: 32 5357 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5389 XbaI T'CTAG_A Cuts at: 2725 2725 Size: 5389 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5389 XmaI C'CCGG_G Cuts at: 1870 2714 1870 Size: 844 4545 XmnI GAAnn'nnTTC Cuts at: 2670 3518 2670 Size: 848 4541 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3197 276 Size: 53 83 186 936 1663 2468 Fragments arranged by size: 2468 1663 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI