(Circular) (Six-base) MAPSORT of: pFN21AF0188.seq Check: 3255 from: 1 to: 8536 pFN21AF0188 8536 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5182 5182 Size: 8536 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6346 278 Size: 53 83 186 936 4810 2468 Fragments arranged by size: 4810 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5066 5879 1937 Size: 3129 813 4594 Fragments arranged by size: 4594 3129 813 Acc65I G'GTAC_C Cuts at: 2857 4266 5857 2857 Size: 1409 1591 5536 Fragments arranged by size: 5536 1591 1409 AclI AA'CG_TT Cuts at: 1563 4167 6663 7036 1563 Size: 2604 2496 373 3063 Fragments arranged by size: 3063 2604 2496 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2375 2807 3041 3837 3918 4158 4216 6599 Size: 743 432 234 796 81 240 58 2383 Cuts at: 6599 7983 8251 1632 Size: 1384 268 1917 Fragments arranged by size: 2383 1917 1384 796 743 432 268 240 234 81 58 AflII C'TTAA_G Cuts at: 820 1017 4819 820 Size: 197 3802 4537 Fragments arranged by size: 4537 3802 197 AflIII A'CryG_T Cuts at: 1203 7435 8412 1203 Size: 6232 977 1327 Fragments arranged by size: 6232 1327 977 AgeI A'CCGG_T Cuts at: 7367 7367 Size: 8536 AhdI GACnn_n'nnGTC Cuts at: 7265 7265 Size: 8536 AleI CACnn'nnGTG Cuts at: 1166 4821 5015 1166 Size: 3655 194 4687 Fragments arranged by size: 4687 3655 194 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2660 3311 5378 5562 1184 Size: 1476 651 2067 184 4158 Fragments arranged by size: 4158 2067 1476 651 184 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4448 4480 4448 Size: 32 8504 AlwNI CAG_nnn'CTG Cuts at: 3279 3548 4375 4408 4578 5701 6279 7851 3279 Size: 269 827 33 170 1123 578 1572 3964 Fragments arranged by size: 3964 1572 1123 827 578 269 170 33 ApaI G_GGCC'C Cuts at: 3028 3207 3028 Size: 179 8357 ApaLI G'TGCA_C Cuts at: 6593 7749 6593 Size: 1156 7380 ApoI r'AATT_y Cuts at: 1493 2376 3493 4760 5595 5845 6071 6223 8332 Size: 883 1117 1267 835 250 226 152 2109 Cuts at: 8332 1493 Size: 1697 Fragments arranged by size: 2109 1697 1267 1117 883 835 250 226 152 AseI AT'TA_AT Cuts at: 160 1032 7090 160 Size: 872 6058 1606 Fragments arranged by size: 6058 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8536 AvaI C'yCGr_G Cuts at: 1870 1943 2072 2264 2561 3443 4894 5861 1870 Size: 73 129 192 297 882 1451 967 4545 Fragments arranged by size: 4545 1451 967 882 297 192 129 73 AvrII C'CTAG_G Cuts at: 3014 3014 Size: 8536 BamHI G'GATC_C Cuts at: 2339 3562 5866 6233 2339 Size: 1223 2304 367 4642 Fragments arranged by size: 4642 2304 1223 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2032 2255 2516 2695 2857 Size: 325 217 627 245 223 261 179 162 Cuts at: 2857 3945 4057 4266 4898 5673 5857 7312 618 Size: 1088 112 209 632 775 184 1455 1842 Fragments arranged by size: 1842 1455 1088 775 632 627 325 261 245 223 217 209 184 179 162 112 BanII G_rGCy'C Cuts at: 1391 2473 2489 2798 2936 3028 3207 3425 3434 Size: 1082 16 309 138 92 179 218 9 Cuts at: 3434 3443 3746 3937 4194 4914 5855 1391 Size: 9 303 191 257 720 941 4072 Fragments arranged by size: 4072 1082 941 720 309 303 257 218 191 179 138 92 16 9 9 BbeI G_GCGC'C Cuts at: 3949 4902 3949 Size: 953 7583 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2956 3825 3855 3906 4248 5526 928 Size: 971 1057 869 30 51 342 1278 3938 Fragments arranged by size: 3938 1278 1057 971 869 342 51 30 BbvCI CC'TCA_GC Cuts at: 5444 5444 Size: 8536 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5518 5552 6727 6761 1903 Size: 34 3581 34 1175 34 3678 Fragments arranged by size: 3678 3581 1175 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6428 7638 6428 Size: 1210 7326 BclI T'GATC_A Cuts at: 1548 4308 5139 1548 Size: 2760 831 4945 Fragments arranged by size: 4945 2760 831 BfrBI ATG'CAT Cuts at: 3145 3145 Size: 8536 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2175 7147 136 Size: 107 122 71 631 605 503 4972 1525 Fragments arranged by size: 4972 1525 631 605 503 122 107 71 BglII A'GATC_T Cuts at: 5025 8531 5025 Size: 3506 5030 BlpI GC'TnA_GC Cuts at: 3187 5953 3187 Size: 2766 5770 Bme1580I G_kGCm'C Cuts at: 1790 2035 2260 2521 2543 2700 3028 3207 3484 Size: 245 225 261 22 157 328 179 277 Cuts at: 3484 5678 6597 7753 1790 Size: 2194 919 1156 2573 Fragments arranged by size: 2573 2194 1156 919 328 277 261 245 225 179 157 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8536 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4385 4527 6267 Size: 416 53 83 399 24 2968 142 1740 Cuts at: 6267 7225 442 Size: 958 1753 Fragments arranged by size: 2968 1753 1740 958 416 399 142 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4727 1056 Size: 3671 4865 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 2947 3019 3057 3085 3697 3897 4504 Size: 824 884 72 38 28 612 200 607 Cuts at: 4504 4928 5183 7196 1239 Size: 424 255 2013 2579 Fragments arranged by size: 2579 2013 884 824 612 607 424 255 200 72 38 28 Bpu10I CC'TnA_GC Cuts at: 2494 2873 3375 5444 2494 Size: 379 502 2069 5586 Fragments arranged by size: 5586 2069 502 379 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4808 5045 6661 7526 7824 4808 Size: 237 1616 865 298 5520 Fragments arranged by size: 5520 1616 865 298 237 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7199 882 Size: 617 5700 2219 Fragments arranged by size: 5700 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6327 493 Size: 711 5123 2702 Fragments arranged by size: 5123 2702 711 BsaBI GATnn'nnATC Cuts at: 4985 8530 4985 Size: 3545 4991 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2118 3946 4141 4899 Size: 53 83 186 936 585 1828 195 758 Cuts at: 4899 6343 6725 7360 275 Size: 1444 382 635 1451 Fragments arranged by size: 1828 1451 1444 936 758 635 585 382 195 186 83 53 BsaWI w'CCGG_w Cuts at: 2328 5829 6969 7367 7641 7788 8199 2328 Size: 3501 1140 398 274 147 411 2665 Fragments arranged by size: 3501 2665 1140 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2995 3025 3071 3101 1178 Size: 30 165 30 1592 30 46 30 6613 Fragments arranged by size: 6613 1592 165 46 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2290 2770 2944 3016 3668 3748 4238 4553 Size: 1104 480 174 72 652 80 490 315 Cuts at: 4553 4565 1186 Size: 12 5157 Fragments arranged by size: 5157 1104 652 490 480 315 174 80 72 12 BseYI C'CCAG_C Cuts at: 2209 5208 5748 7739 8235 2209 Size: 2999 540 1991 496 2510 Fragments arranged by size: 2999 2510 1991 540 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2118 3148 3265 5039 5568 5687 1163 Size: 573 382 1030 117 1774 529 119 4012 Fragments arranged by size: 4012 1774 1030 573 529 382 119 117 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5986 6747 7775 1540 Size: 275 186 3985 761 1028 2301 Fragments arranged by size: 3985 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2594 2768 2798 3110 4115 4914 6597 6682 7753 Size: 174 30 312 1005 799 1683 85 1071 Cuts at: 7753 2594 Size: 3377 Fragments arranged by size: 3377 1683 1071 1005 799 312 174 85 30 BsmI GAATG_Cn' Cuts at: 3913 6047 6140 3913 Size: 2134 93 6309 Fragments arranged by size: 6309 2134 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3103 3745 3103 Size: 642 7894 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2260 2473 2489 2521 2543 2594 Size: 399 245 225 213 16 32 22 51 Cuts at: 2594 2700 2768 2798 2936 3028 3110 3207 3425 Size: 106 68 30 138 92 82 97 218 Cuts at: 3425 3434 3443 3484 3746 3937 4115 4194 4914 Size: 9 9 41 262 191 178 79 720 Cuts at: 4914 5678 5855 6597 6682 7753 1391 Size: 764 177 742 85 1071 2174 Fragments arranged by size: 2174 1071 764 742 720 399 262 245 225 218 213 191 178 177 138 106 97 92 85 82 79 68 51 41 32 30 22 16 9 9 BspEI T'CCGG_A Cuts at: 2328 5829 8199 2328 Size: 3501 2370 2665 Fragments arranged by size: 3501 2665 2370 BspHI T'CATG_A Cuts at: 4923 6425 8155 4923 Size: 1502 1730 5304 Fragments arranged by size: 5304 1730 1502 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3170 4278 5182 5355 5746 5891 844 Size: 2326 1108 904 173 391 145 3489 Fragments arranged by size: 3489 2326 1108 904 391 173 145 BsrBI CCG'CTC Cuts at: 1394 1823 2269 2601 4636 6423 1394 Size: 429 446 332 2035 1787 3507 Fragments arranged by size: 3507 2035 1787 446 429 332 BsrDI GCAATG_nn' Cuts at: 65 1248 2986 7031 7205 65 Size: 1183 1738 4045 174 1396 Fragments arranged by size: 4045 1738 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3069 3629 4024 4143 5379 7180 7367 Size: 110 1259 560 395 119 1236 1801 187 Cuts at: 7367 1700 Size: 2869 Fragments arranged by size: 2869 1801 1259 1236 560 395 187 119 110 BsrGI T'GTAC_A Cuts at: 96 4641 96 Size: 4545 3991 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8536 BssSI C'ACGA_G Cuts at: 2717 6596 7608 2717 Size: 3879 1012 3645 Fragments arranged by size: 3879 3645 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2516 3455 4597 794 Size: 1722 939 1142 4733 Fragments arranged by size: 4733 1722 1142 939 BstBI TT'CG_AA Cuts at: 7340 7340 Size: 8536 BstEII G'GTnAC_C Cuts at: 1184 2128 1184 Size: 944 7592 BstXI CCAn_nnnn'nTGG Cuts at: 2287 2535 2903 4126 2287 Size: 248 368 1223 6697 Fragments arranged by size: 6697 1223 368 248 BstYI r'GATC_y Cuts at: 1972 2339 3562 4832 5025 5866 6233 6618 6635 Size: 367 1223 1270 193 841 367 385 17 Cuts at: 6635 8087 8173 8185 8531 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1270 1223 841 385 367 367 346 193 86 17 12 BstZ17I GTA'TAC Cuts at: 5067 5067 Size: 8536 Bsu36I CC'TnA_GG Cuts at: 2522 2834 4655 2522 Size: 312 1821 6403 Fragments arranged by size: 6403 1821 312 BtgI C'CryG_G Cuts at: 513 1065 2280 3168 4359 5679 513 Size: 552 1215 888 1191 1320 3370 Fragments arranged by size: 3370 1320 1215 1191 888 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4972 487 Size: 683 56 3746 4051 Fragments arranged by size: 4051 3746 683 56 BtsI GCAGTG_nn' Cuts at: 2055 3153 3222 4450 6052 6846 6866 2055 Size: 1098 69 1228 1602 794 20 3725 Fragments arranged by size: 3725 1602 1228 1098 794 69 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8536 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4717 4752 529 Size: 35 4153 35 4313 Fragments arranged by size: 4313 4153 35 35 DraI TTT'AAA Cuts at: 5840 5840 Size: 8536 DraIII CAC_nnn'GTG Cuts at: 3584 4057 3584 Size: 473 8063 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7543 809 Size: 507 6227 1802 Fragments arranged by size: 6227 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2689 3755 3767 Size: 54 1425 50 275 18 859 1066 12 Cuts at: 3767 3784 3792 4293 5049 5983 6872 8501 8 Size: 17 8 501 756 934 889 1629 43 Fragments arranged by size: 1629 1425 1066 934 889 859 756 501 275 54 50 43 18 17 12 8 EagI C'GGCC_G Cuts at: 1537 1812 5983 1537 Size: 275 4171 4090 Fragments arranged by size: 4171 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2240 2786 3516 4497 5801 6466 1353 Size: 887 546 730 981 1304 665 3423 Fragments arranged by size: 3423 1304 981 887 730 665 546 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5786 7123 7497 7643 387 Size: 1064 138 4197 1337 374 146 1280 Fragments arranged by size: 4197 1337 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2796 4912 2796 Size: 2116 6420 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2375 2807 2947 3019 3041 3057 Size: 393 431 312 432 140 72 22 16 Cuts at: 3057 3085 3697 3837 3897 3918 4158 4216 4504 Size: 28 612 140 60 21 240 58 288 Cuts at: 4504 4928 5183 6599 7196 7983 8251 1239 Size: 424 255 1416 597 787 268 1524 Fragments arranged by size: 1524 1416 787 612 597 432 431 424 393 312 288 268 255 240 140 140 72 60 58 28 22 21 16 EcoNI CCTnn'n_nnAGG Cuts at: 3001 4250 5812 3001 Size: 1249 1562 5725 Fragments arranged by size: 5725 1562 1249 EcoO109I rG'GnC_Cy Cuts at: 2081 2725 2868 3024 3025 3203 3204 3545 3601 Size: 644 143 156 1 178 1 341 56 Cuts at: 3601 3865 5344 5698 2081 Size: 264 1479 354 4919 Fragments arranged by size: 4919 1479 644 354 341 264 178 156 143 56 1 1 EcoRI G'AATT_C Cuts at: 2376 3493 5845 6223 2376 Size: 1117 2352 378 4689 Fragments arranged by size: 4689 2352 1117 378 EcoRV GAT'ATC Cuts at: 5224 5224 Size: 8536 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2815 3900 2815 Size: 1085 7451 FspI TGC'GCA Cuts at: 4333 4719 5354 7042 4333 Size: 386 635 1688 5827 Fragments arranged by size: 5827 1688 635 386 HaeII r_GCGC'y Cuts at: 1678 2460 3949 4902 5722 7683 1678 Size: 782 1489 953 820 1961 2531 Fragments arranged by size: 2531 1961 1489 953 820 782 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2995 3027 3071 3103 5208 Size: 32 135 32 1423 32 44 32 2105 Cuts at: 5208 5222 5240 5254 7177 7209 7251 7283 1373 Size: 14 18 14 1923 32 42 32 2626 Fragments arranged by size: 2626 2105 1923 1423 135 44 42 32 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 3363 5880 6126 669 Size: 988 281 1425 2517 246 3079 Fragments arranged by size: 3079 2517 1425 988 281 246 HindIII A'AGCT_T Cuts at: 748 2216 2900 748 Size: 1468 684 6384 Fragments arranged by size: 6384 1468 684 HpaI GTT'AAC Cuts at: 6126 6126 Size: 8536 KasI G'GCGC_C Cuts at: 3945 4898 3945 Size: 953 7583 KpnI G_GTAC'C Cuts at: 2861 4270 5861 2861 Size: 1409 1591 5536 Fragments arranged by size: 5536 1591 1409 MfeI C'AATT_G Cuts at: 6135 6135 Size: 8536 MluI A'CGCG_T Cuts at: 8412 8412 Size: 8536 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2222 2580 4107 4188 4443 4491 4705 7650 Size: 917 358 1527 81 255 48 214 2945 Cuts at: 7650 7834 1305 Size: 184 2007 Fragments arranged by size: 2945 2007 1527 917 358 255 214 184 81 48 MscI TGG'CCA Cuts at: 10 64 1489 1832 2691 3794 4295 10 Size: 54 1425 343 859 1103 501 4251 Fragments arranged by size: 4251 1425 1103 859 501 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3585 3620 3956 4703 4821 5015 5729 Size: 648 2419 35 336 747 118 194 714 Cuts at: 5729 6494 6853 7012 518 Size: 765 359 159 2042 Fragments arranged by size: 2419 2042 765 747 714 648 359 336 194 159 118 35 MspA1I CmG'CkG Cuts at: 1606 2587 3462 3790 4291 5041 5047 5299 5326 Size: 981 875 328 501 750 6 252 27 Cuts at: 5326 5952 6629 7777 8022 1606 Size: 626 677 1148 245 2120 Fragments arranged by size: 2120 1148 981 875 750 677 626 501 328 252 245 27 6 NaeI GCC'GGC Cuts at: 3071 3631 4145 5381 3071 Size: 560 514 1236 6226 Fragments arranged by size: 6226 1236 560 514 NarI GG'CG_CC Cuts at: 3946 4899 3946 Size: 953 7583 NcoI C'CATG_G Cuts at: 513 1065 2280 4359 513 Size: 552 1215 2079 4690 Fragments arranged by size: 4690 2079 1215 552 NdeI CA'TA_TG Cuts at: 387 5110 387 Size: 4723 3813 NgoMIV G'CCGG_C Cuts at: 3069 3629 4143 5379 3069 Size: 560 514 1236 6226 Fragments arranged by size: 6226 1236 560 514 NheI G'CTAG_C Cuts at: 1052 4723 1052 Size: 3671 4865 NotI GC'GGCC_GC Cuts at: 5983 5983 Size: 8536 NsiI A_TGCA'T Cuts at: 3147 3147 Size: 8536 NspI r_CATG'y Cuts at: 1132 1229 2514 5365 5894 7439 1132 Size: 97 1285 2851 529 1545 2229 Fragments arranged by size: 2851 2229 1545 1285 529 97 PasI CC'CwG_GG Cuts at: 1348 2028 1348 Size: 680 7856 PciI A'CATG_T Cuts at: 7435 7435 Size: 8536 PflMI CCAn_nnn'nTGG Cuts at: 1108 2281 2513 2925 3035 3350 4375 4408 5326 Size: 1173 232 412 110 315 1025 33 918 Cuts at: 5326 1108 Size: 4318 Fragments arranged by size: 4318 1173 1025 918 412 315 232 110 33 PfoI T'CCnGG_A Cuts at: 3101 3312 4614 3101 Size: 211 1302 7023 Fragments arranged by size: 7023 1302 211 PmeI GTTT'AAAC Cuts at: 5840 5840 Size: 8536 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6605 6637 8118 8150 6605 Size: 32 1481 32 6991 Fragments arranged by size: 6991 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2725 2868 3601 3865 5344 2725 Size: 143 733 264 1479 5917 Fragments arranged by size: 5917 1479 733 264 143 PshAI GACnn'nnGTC Cuts at: 1613 4607 1613 Size: 2994 5542 PsiI TTA'TAA Cuts at: 6106 6106 Size: 8536 PspOMI G'GGCC_C Cuts at: 3024 3203 3024 Size: 179 8357 PspXI vC'TCGA_Gb Cuts at: 2561 2561 Size: 8536 PstI C_TGCA'G Cuts at: 830 2168 3835 4275 4974 5046 5541 5888 830 Size: 1338 1667 440 699 72 495 347 3478 Fragments arranged by size: 3478 1667 1338 699 495 440 347 72 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8536 PvuII CAG'CTG Cuts at: 3462 3790 4291 5047 5326 3462 Size: 328 501 756 279 6672 Fragments arranged by size: 6672 756 501 328 279 SacI G_AGCT'C Cuts at: 2798 4914 2798 Size: 2116 6420 SalI G'TCGA_C Cuts at: 1936 5878 1936 Size: 3942 4594 SanDI GG'GwC_CC Cuts at: 2725 2725 Size: 8536 SapI GCTCTTCn'nnn_ Cuts at: 2786 3516 4497 5801 2786 Size: 730 981 1304 5521 Fragments arranged by size: 5521 1304 981 730 SbfI CC_TGCA'GG Cuts at: 5888 5888 Size: 8536 ScaI AGT'ACT Cuts at: 4880 6784 8496 4880 Size: 1904 1712 4920 Fragments arranged by size: 4920 1904 1712 SexAI A'CCwGG_T Cuts at: 4053 4053 Size: 8536 SfcI C'TryA_G Cuts at: 826 1045 2164 2749 3831 4271 4970 5042 5537 Size: 219 1119 585 1082 440 699 72 495 Cuts at: 5537 5884 7019 7700 7891 826 Size: 347 1135 681 191 1471 Fragments arranged by size: 1471 1135 1119 1082 699 681 585 495 440 347 219 191 72 SfoI GGC'GCC Cuts at: 3947 4900 3947 Size: 953 7583 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8536 SgrAI Cr'CCGG_yG Cuts at: 1700 4143 1700 Size: 2443 6093 SmaI CCC'GGG Cuts at: 1872 3445 4896 5863 1872 Size: 1573 1451 967 4545 Fragments arranged by size: 4545 1573 1451 967 SmlI C'TyrA_G Cuts at: 820 1017 1943 2561 4787 4819 5060 6640 7541 Size: 197 926 618 2226 32 241 1580 901 Cuts at: 7541 7803 820 Size: 262 1553 Fragments arranged by size: 2226 1580 1553 926 901 618 262 241 197 32 SnaBI TAC'GTA Cuts at: 493 6327 493 Size: 5834 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8536 SphI G_CATG'C Cuts at: 1132 5894 1132 Size: 4762 3774 SspI AAT'ATT Cuts at: 5 52 6460 5 Size: 47 6408 2081 Fragments arranged by size: 6408 2081 47 StuI AGG'CCT Cuts at: 1517 3763 5543 1517 Size: 2246 1780 4510 Fragments arranged by size: 4510 2246 1780 StyI C'CwwG_G Cuts at: 513 1065 2280 2946 3014 3758 4359 5975 513 Size: 552 1215 666 68 744 601 1616 3074 Fragments arranged by size: 3074 1616 1215 744 666 601 552 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2204 2689 3424 5247 6563 6580 6733 Size: 1255 624 485 735 1823 1316 17 153 Cuts at: 6733 6918 7363 325 Size: 185 445 1498 Fragments arranged by size: 1823 1498 1316 1255 735 624 485 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4641 4797 4878 Size: 274 80 33 51 1443 2664 156 81 Cuts at: 4878 4938 5367 6782 8494 96 Size: 60 429 1415 1712 138 Fragments arranged by size: 2664 1712 1443 1415 429 274 156 138 81 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4418 4450 8496 8528 4418 Size: 32 4046 32 4426 Fragments arranged by size: 4426 4046 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2960 1316 Size: 1644 6892 XbaI T'CTAG_A Cuts at: 5872 5872 Size: 8536 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2287 5125 2287 Size: 2838 5698 XhoI C'TCGA_G Cuts at: 1943 2561 1943 Size: 618 7918 XmaI C'CCGG_G Cuts at: 1870 3443 4894 5861 1870 Size: 1573 1451 967 4545 Fragments arranged by size: 4545 1573 1451 967 XmnI GAAnn'nnTTC Cuts at: 5247 6665 5247 Size: 1418 7118 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6344 276 Size: 53 83 186 936 4810 2468 Fragments arranged by size: 4810 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BplI BsiWI FseI FspAI NruI PacI PmlI PsrI RsrII SacII SfiI SrfI SwaI