Restriction map for
pFN21AF0188
(Linear) (Six-base) MAPSORT of: FLJ00188.seq Check: 3608 from: 1 to: 3831
FLJ00188.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3177 3831
Size: 3177 654
AccI GT'mk_AC
Cuts at: 0 3061 3831
Size: 3061 770
Acc65I G'GTAC_C
Cuts at: 0 852 2261 3831
Size: 852 1409 1570
Fragments arranged by size:
1570 1409 852
AclI AA'CG_TT
Cuts at: 0 2162 3831
Size: 2162 1669
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 802 1036 1832 1913 2153 2211 3831
Size: 370 432 234 796 81 240 58 1620
Fragments arranged by size:
1620 796 432 370 240 234 81 58
AflII C'TTAA_G
Cuts at: 0 2814 3831
Size: 2814 1017
AleI CACnn'nnGTG
Cuts at: 0 2816 3010 3831
Size: 2816 194 821
Fragments arranged by size:
2816 821 194
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 655 1306 3373 3557 3831
Size: 655 651 2067 184 274
Fragments arranged by size:
2067 655 651 274 184
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2443 2475 3831
Size: 2443 32 1356
Fragments arranged by size:
2443 1356 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1274 1543 2370 2403 2573 3696 3831
Size: 1274 269 827 33 170 1123 135
Fragments arranged by size:
1274 1123 827 269 170 135 33
ApaI G_GGCC'C
Cuts at: 0 1023 1202 3831
Size: 1023 179 2629
Fragments arranged by size:
2629 1023 179
ApoI r'AATT_y
Cuts at: 0 371 1488 2755 3590 3831
Size: 371 1117 1267 835 241
Fragments arranged by size:
1267 1117 835 371 241
AvaI C'yCGr_G
Cuts at: 0 67 259 556 1438 2889 3831
Size: 67 192 297 882 1451 942
Fragments arranged by size:
1451 942 882 297 192 67
AvrII C'CTAG_G
Cuts at: 0 1009 3831
Size: 1009 2822
BamHI G'GATC_C
Cuts at: 0 334 1557 3831
Size: 334 1223 2274
Fragments arranged by size:
2274 1223 334
BanI G'GyrC_C
Cuts at: 0 27 250 511 690 852 1940 2052 2261
Size: 27 223 261 179 162 1088 112 209
Cuts at: 2261 2893 3668 3831
Size: 632 775 163
Fragments arranged by size:
1088 775 632 261 223 209 179 163
162 112 27
BanII G_rGCy'C
Cuts at: 0 468 484 793 931 1023 1202 1420 1429
Size: 468 16 309 138 92 179 218 9
Cuts at: 1429 1438 1741 1932 2189 2909 3831
Size: 9 303 191 257 720 922
Fragments arranged by size:
922 720 468 309 303 257 218 191
179 138 92 16 9 9
BbeI G_GCGC'C
Cuts at: 0 1944 2897 3831
Size: 1944 953 934
Fragments arranged by size:
1944 953 934
BbsI GAAGACnn'nnnn_
Cuts at: 0 951 1820 1850 1901 2243 3521 3831
Size: 951 869 30 51 342 1278 310
Fragments arranged by size:
1278 951 869 342 310 51 30
BbvCI CC'TCA_GC
Cuts at: 0 3439 3831
Size: 3439 392
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3513 3547 3831
Size: 3513 34 284
Fragments arranged by size:
3513 284 34
BclI T'GATC_A
Cuts at: 0 2303 3134 3831
Size: 2303 831 697
Fragments arranged by size:
2303 831 697
BfrBI ATG'CAT
Cuts at: 0 1140 3831
Size: 1140 2691
BglI GCCn_nnn'nGGC
Cuts at: 0 170 3831
Size: 170 3661
BglII A'GATC_T
Cuts at: 0 3020 3831
Size: 3020 811
BlpI GC'TnA_GC
Cuts at: 0 1182 3831
Size: 1182 2649
Bme1580I G_kGCm'C
Cuts at: 0 30 255 516 538 695 1023 1202 1479
Size: 30 225 261 22 157 328 179 277
Cuts at: 1479 3673 3831
Size: 2194 158
Fragments arranged by size:
2194 328 277 261 225 179 158 157
30 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 2380 2522 3831
Size: 2380 142 1309
Fragments arranged by size:
2380 1309 142
BmtI G_CTAG'C
Cuts at: 0 2722 3831
Size: 2722 1109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 942 1014 1052 1080 1692 1892 2499
Size: 58 884 72 38 28 612 200 607
Cuts at: 2499 2923 3178 3831
Size: 424 255 653
Fragments arranged by size:
884 653 612 607 424 255 200 72
58 38 28
Bpu10I CC'TnA_GC
Cuts at: 0 489 868 1370 3439 3831
Size: 489 379 502 2069 392
Fragments arranged by size:
2069 502 489 392 379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2803 3040 3831
Size: 2803 237 791
Fragments arranged by size:
2803 791 237
BsaBI GATnn'nnATC
Cuts at: 0 2980 3831
Size: 2980 851
BsaHI Gr'CG_yC
Cuts at: 0 113 1941 2136 2894 3831
Size: 113 1828 195 758 937
Fragments arranged by size:
1828 937 758 195 113
BsaWI w'CCGG_w
Cuts at: 0 323 3824 3831
Size: 323 3501 7
Fragments arranged by size:
3501 323 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 990 1020 1066 1096 3831
Size: 990 30 46 30 2735
Fragments arranged by size:
2735 990 46 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 285 765 939 1011 1663 1743 2233 2548
Size: 285 480 174 72 652 80 490 315
Cuts at: 2548 2560 3831
Size: 12 1271
Fragments arranged by size:
1271 652 490 480 315 285 174 80
72 12
BseYI C'CCAG_C
Cuts at: 0 204 3203 3743 3831
Size: 204 2999 540 88
Fragments arranged by size:
2999 540 204 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 1143 1260 3034 3563 3682 3831
Size: 113 1030 117 1774 529 119 149
Fragments arranged by size:
1774 1030 529 149 119 117 113
BsiHKAI G_wGCw'C
Cuts at: 0 589 763 793 1105 2110 2909 3831
Size: 589 174 30 312 1005 799 922
Fragments arranged by size:
1005 922 799 589 312 174 30
BsmI GAATG_Cn'
Cuts at: 0 1908 3831
Size: 1908 1923
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1098 1740 3831
Size: 1098 642 2091
Fragments arranged by size:
2091 1098 642
Bsp1286I G_dGCh'C
Cuts at: 0 30 255 468 484 516 538 589 695
Size: 30 225 213 16 32 22 51 106
Cuts at: 695 763 793 931 1023 1105 1202 1420 1429
Size: 68 30 138 92 82 97 218 9
Cuts at: 1429 1438 1479 1741 1932 2110 2189 2909 3673
Size: 9 41 262 191 178 79 720 764
Cuts at: 3673 3831
Size: 158
Fragments arranged by size:
764 720 262 225 218 213 191 178
158 138 106 97 92 82 79 68
51 41 32 30 30 22 16 9
9
BspEI T'CCGG_A
Cuts at: 0 323 3824 3831
Size: 323 3501 7
Fragments arranged by size:
3501 323 7
BspHI T'CATG_A
Cuts at: 0 2918 3831
Size: 2918 913
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1165 2273 3177 3350 3741 3831
Size: 1165 1108 904 173 391 90
Fragments arranged by size:
1165 1108 904 391 173 90
BsrBI CCG'CTC
Cuts at: 0 264 596 2631 3831
Size: 264 332 2035 1200
Fragments arranged by size:
2035 1200 332 264
BsrDI GCAATG_nn'
Cuts at: 0 981 3831
Size: 981 2850
BsrFI r'CCGG_y
Cuts at: 0 1064 1624 2019 2138 3374 3831
Size: 1064 560 395 119 1236 457
Fragments arranged by size:
1236 1064 560 457 395 119
BsrGI T'GTAC_A
Cuts at: 0 2636 3831
Size: 2636 1195
BssSI C'ACGA_G
Cuts at: 0 712 3831
Size: 712 3119
BstAPI GCAn_nnn'nTGC
Cuts at: 0 511 1450 2592 3831
Size: 511 939 1142 1239
Fragments arranged by size:
1239 1142 939 511
BstEII G'GTnAC_C
Cuts at: 0 123 3831
Size: 123 3708
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 530 898 2121 3831
Size: 282 248 368 1223 1710
Fragments arranged by size:
1710 1223 368 282 248
BstYI r'GATC_y
Cuts at: 0 334 1557 2827 3020 3831
Size: 334 1223 1270 193 811
Fragments arranged by size:
1270 1223 811 334 193
BstZ17I GTA'TAC
Cuts at: 0 3062 3831
Size: 3062 769
Bsu36I CC'TnA_GG
Cuts at: 0 517 829 2650 3831
Size: 517 312 1821 1181
Fragments arranged by size:
1821 1181 517 312
BtgI C'CryG_G
Cuts at: 0 275 1163 2354 3674 3831
Size: 275 888 1191 1320 157
Fragments arranged by size:
1320 1191 888 275 157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2967 3831
Size: 2967 864
BtsI GCAGTG_nn'
Cuts at: 0 50 1148 1217 2445 3831
Size: 50 1098 69 1228 1386
Fragments arranged by size:
1386 1228 1098 69 50
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2712 2747 3831
Size: 2712 35 1084
Fragments arranged by size:
2712 1084 35
DraIII CAC_nnn'GTG
Cuts at: 0 1579 2052 3831
Size: 1579 473 1779
Fragments arranged by size:
1779 1579 473
EaeI y'GGCC_r
Cuts at: 0 684 1750 1762 1779 1787 2288 3044 3831
Size: 684 1066 12 17 8 501 756 787
Fragments arranged by size:
1066 787 756 684 501 17 12 8
EarI CTCTTCn'nnn_
Cuts at: 0 235 781 1511 2492 3796 3831
Size: 235 546 730 981 1304 35
Fragments arranged by size:
1304 981 730 546 235 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3781 3831
Size: 3781 50
EcoICRI GAG'CTC
Cuts at: 0 791 2907 3831
Size: 791 2116 924
Fragments arranged by size:
2116 924 791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 370 802 942 1014 1036 1052 1080
Size: 58 312 432 140 72 22 16 28
Cuts at: 1080 1692 1832 1892 1913 2153 2211 2499 2923
Size: 612 140 60 21 240 58 288 424
Cuts at: 2923 3178 3831
Size: 255 653
Fragments arranged by size:
653 612 432 424 312 288 255 240
140 140 72 60 58 58 28 22
21 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 996 2245 3807 3831
Size: 996 1249 1562 24
Fragments arranged by size:
1562 1249 996 24
EcoO109I rG'GnC_Cy
Cuts at: 0 76 720 863 1019 1020 1198 1199 1540
Size: 76 644 143 156 1 178 1 341
Cuts at: 1540 1596 1860 3339 3693 3831
Size: 56 264 1479 354 138
Fragments arranged by size:
1479 644 354 341 264 178 156 143
138 76 56 1 1
EcoRI G'AATT_C
Cuts at: 0 371 1488 3831
Size: 371 1117 2343
Fragments arranged by size:
2343 1117 371
EcoRV GAT'ATC
Cuts at: 0 3219 3831
Size: 3219 612
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 810 1895 3831
Size: 810 1085 1936
Fragments arranged by size:
1936 1085 810
FspI TGC'GCA
Cuts at: 0 2328 2714 3349 3831
Size: 2328 386 635 482
Fragments arranged by size:
2328 635 482 386
HaeII r_GCGC'y
Cuts at: 0 455 1944 2897 3717 3831
Size: 455 1489 953 820 114
Fragments arranged by size:
1489 953 820 455 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 990 1022 1066 1098 3203 3217 3235 3249
Size: 990 32 44 32 2105 14 18 14
Cuts at: 3249 3831
Size: 582
Fragments arranged by size:
2105 990 582 44 32 32 18 14
14
HincII GTy'rAC
Cuts at: 0 1358 3831
Size: 1358 2473
HindIII A'AGCT_T
Cuts at: 0 211 895 3831
Size: 211 684 2936
Fragments arranged by size:
2936 684 211
KasI G'GCGC_C
Cuts at: 0 1940 2893 3831
Size: 1940 953 938
Fragments arranged by size:
1940 953 938
KpnI G_GTAC'C
Cuts at: 0 856 2265 3831
Size: 856 1409 1566
Fragments arranged by size:
1566 1409 856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 575 2102 2183 2438 2486 2700 3831
Size: 217 358 1527 81 255 48 214 1131
Fragments arranged by size:
1527 1131 358 255 217 214 81 48
MscI TGG'CCA
Cuts at: 0 686 1789 2290 3831
Size: 686 1103 501 1541
Fragments arranged by size:
1541 1103 686 501
MslI CAynn'nnrTG
Cuts at: 0 1580 1615 1951 2698 2816 3010 3724 3831
Size: 1580 35 336 747 118 194 714 107
Fragments arranged by size:
1580 747 714 336 194 118 107 35
MspA1I CmG'CkG
Cuts at: 0 582 1457 1785 2286 3036 3042 3294 3321
Size: 582 875 328 501 750 6 252 27
Cuts at: 3321 3831
Size: 510
Fragments arranged by size:
875 750 582 510 501 328 252 27
6
NaeI GCC'GGC
Cuts at: 0 1066 1626 2140 3376 3831
Size: 1066 560 514 1236 455
Fragments arranged by size:
1236 1066 560 514 455
NarI GG'CG_CC
Cuts at: 0 1941 2894 3831
Size: 1941 953 937
Fragments arranged by size:
1941 953 937
NcoI C'CATG_G
Cuts at: 0 275 2354 3831
Size: 275 2079 1477
Fragments arranged by size:
2079 1477 275
NdeI CA'TA_TG
Cuts at: 0 3105 3831
Size: 3105 726
NgoMIV G'CCGG_C
Cuts at: 0 1064 1624 2138 3374 3831
Size: 1064 560 514 1236 457
Fragments arranged by size:
1236 1064 560 514 457
NheI G'CTAG_C
Cuts at: 0 2718 3831
Size: 2718 1113
NsiI A_TGCA'T
Cuts at: 0 1142 3831
Size: 1142 2689
NspI r_CATG'y
Cuts at: 0 509 3360 3831
Size: 509 2851 471
Fragments arranged by size:
2851 509 471
PasI CC'CwG_GG
Cuts at: 0 23 3831
Size: 23 3808
PflMI CCAn_nnn'nTGG
Cuts at: 0 276 508 920 1030 1345 2370 2403 3321
Size: 276 232 412 110 315 1025 33 918
Cuts at: 3321 3831
Size: 510
Fragments arranged by size:
1025 918 510 412 315 276 232 110
33
PfoI T'CCnGG_A
Cuts at: 0 1096 1307 2609 3831
Size: 1096 211 1302 1222
Fragments arranged by size:
1302 1222 1096 211
PpuMI rG'GwC_Cy
Cuts at: 0 720 863 1596 1860 3339 3831
Size: 720 143 733 264 1479 492
Fragments arranged by size:
1479 733 720 492 264 143
PshAI GACnn'nnGTC
Cuts at: 0 2602 3831
Size: 2602 1229
PspOMI G'GGCC_C
Cuts at: 0 1019 1198 3831
Size: 1019 179 2633
Fragments arranged by size:
2633 1019 179
PspXI vC'TCGA_Gb
Cuts at: 0 556 3831
Size: 556 3275
PstI C_TGCA'G
Cuts at: 0 163 1830 2270 2969 3041 3536 3831
Size: 163 1667 440 699 72 495 295
Fragments arranged by size:
1667 699 495 440 295 163 72
PvuII CAG'CTG
Cuts at: 0 1457 1785 2286 3042 3321 3831
Size: 1457 328 501 756 279 510
Fragments arranged by size:
1457 756 510 501 328 279
SacI G_AGCT'C
Cuts at: 0 793 2909 3831
Size: 793 2116 922
Fragments arranged by size:
2116 922 793
SanDI GG'GwC_CC
Cuts at: 0 720 3831
Size: 720 3111
SapI GCTCTTCn'nnn_
Cuts at: 0 781 1511 2492 3796 3831
Size: 781 730 981 1304 35
Fragments arranged by size:
1304 981 781 730 35
ScaI AGT'ACT
Cuts at: 0 2875 3831
Size: 2875 956
SexAI A'CCwGG_T
Cuts at: 0 2048 3831
Size: 2048 1783
SfcI C'TryA_G
Cuts at: 0 159 744 1826 2266 2965 3037 3532 3831
Size: 159 585 1082 440 699 72 495 299
Fragments arranged by size:
1082 699 585 495 440 299 159 72
SfoI GGC'GCC
Cuts at: 0 1942 2895 3831
Size: 1942 953 936
Fragments arranged by size:
1942 953 936
SgrAI Cr'CCGG_yG
Cuts at: 0 2138 3831
Size: 2138 1693
SmaI CCC'GGG
Cuts at: 0 1440 2891 3831
Size: 1440 1451 940
Fragments arranged by size:
1451 1440 940
SmlI C'TyrA_G
Cuts at: 0 556 2782 2814 3055 3831
Size: 556 2226 32 241 776
Fragments arranged by size:
2226 776 556 241 32
StuI AGG'CCT
Cuts at: 0 1758 3538 3831
Size: 1758 1780 293
Fragments arranged by size:
1780 1758 293
StyI C'CwwG_G
Cuts at: 0 275 941 1009 1753 2354 3831
Size: 275 666 68 744 601 1477
Fragments arranged by size:
1477 744 666 601 275 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 199 684 1419 3242 3831
Size: 199 485 735 1823 589
Fragments arranged by size:
1823 735 589 485 199
TatI w'GTAC_w
Cuts at: 0 2636 2792 2873 2933 3362 3831
Size: 2636 156 81 60 429 469
Fragments arranged by size:
2636 469 429 156 81 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2413 2445 3831
Size: 2413 32 1386
Fragments arranged by size:
2413 1386 32
Tth111I GACn'n_nGTC
Cuts at: 0 955 3831
Size: 955 2876
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 282 3120 3831
Size: 282 2838 711
Fragments arranged by size:
2838 711 282
XhoI C'TCGA_G
Cuts at: 0 556 3831
Size: 556 3275
XmaI C'CCGG_G
Cuts at: 0 1438 2889 3831
Size: 1438 1451 942
Fragments arranged by size:
1451 1438 942
XmnI GAAnn'nnTTC
Cuts at: 0 3242 3831
Size: 3242 589
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AleI AlfI
AloI AlwNI ApaI ApoI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NsiI NspI PasI PflMI PfoI PpuMI PshAI
PspOMI PspXI PstI PvuII SacI SanDI SapI ScaI
SexAI SfcI SfoI SgrAI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflIII AgeI AhdI ApaLI AscI AseI
AsiSI BaeI BciVI BmgBI BplI BsaI BsaAI BsiEI
BsiWI BssHII BstBI ClaI DraI DrdI EagI FseI
FspAI HpaI MfeI MluI NotI NruI PacI PciI
PmeI PmlI PpiI PsiI PsrI PvuI RsrII SacII
SalI SbfI SfiI SgfI SnaBI SpeI SphI SrfI
SspI SwaI XbaI ZraI