(Circular) (Six-base) MAPSORT of: pFN21AF0107.seq Check: 6158 from: 1 to: 8638 pFN21AF0107 8638 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2899 3892 4149 2899 Size: 993 257 7388 Fragments arranged by size: 7388 993 257 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6448 278 Size: 53 83 186 936 4912 2468 Fragments arranged by size: 4912 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2856 3432 5981 1937 Size: 919 576 2549 4594 Fragments arranged by size: 4594 2549 919 576 Acc65I G'GTAC_C Cuts at: 5959 5959 Size: 8638 AclI AA'CG_TT Cuts at: 1563 5436 6765 7138 1563 Size: 3873 1329 373 3063 Fragments arranged by size: 3873 3063 1329 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2094 3464 4064 4115 5432 6701 8085 8353 Size: 462 1370 600 51 1317 1269 1384 268 Cuts at: 8353 1632 Size: 1917 Fragments arranged by size: 1917 1384 1370 1317 1269 600 462 268 51 AflII C'TTAA_G Cuts at: 820 1017 3371 3814 3854 820 Size: 197 2354 443 40 5604 Fragments arranged by size: 5604 2354 443 197 40 AflIII A'CryG_T Cuts at: 1203 2592 4803 4881 7537 8514 1203 Size: 1389 2211 78 2656 977 1327 Fragments arranged by size: 2656 2211 1389 1327 977 78 AgeI A'CCGG_T Cuts at: 7469 7469 Size: 8638 AhdI GACnn_n'nnGTC Cuts at: 7367 7367 Size: 8638 AleI CACnn'nnGTG Cuts at: 1166 2148 2532 4118 4388 5934 1166 Size: 982 384 1586 270 1546 3870 Fragments arranged by size: 3870 1586 1546 982 384 270 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8638 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5097 5129 5097 Size: 32 8606 AlwNI CAG_nnn'CTG Cuts at: 6381 7953 6381 Size: 1572 7066 ApaLI G'TGCA_C Cuts at: 2141 3879 4264 6695 7851 2141 Size: 1738 385 2431 1156 2928 Fragments arranged by size: 2928 2431 1738 1156 385 ApoI r'AATT_y Cuts at: 1493 3703 4019 5047 5222 5947 6173 6325 8434 Size: 2210 316 1028 175 725 226 152 2109 Cuts at: 8434 1493 Size: 1697 Fragments arranged by size: 2210 2109 1697 1028 725 316 226 175 152 AseI AT'TA_AT Cuts at: 160 1032 2205 7192 160 Size: 872 1173 4987 1606 Fragments arranged by size: 4987 1606 1173 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8638 AvaI C'yCGr_G Cuts at: 1870 1943 3193 5963 1870 Size: 73 1250 2770 4545 Fragments arranged by size: 4545 2770 1250 73 AvrII C'CTAG_G Cuts at: 4861 4861 Size: 8638 BamHI G'GATC_C Cuts at: 5968 6335 5968 Size: 367 8271 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4382 5765 5959 7414 618 Size: 325 217 627 2595 1383 194 1455 1842 Fragments arranged by size: 2595 1842 1455 1383 627 325 217 194 BanII G_rGCy'C Cuts at: 1391 2871 4779 5496 5957 1391 Size: 1480 1908 717 461 4072 Fragments arranged by size: 4072 1908 1480 717 461 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2070 2118 2136 2787 4156 4641 5420 Size: 971 171 48 18 651 1369 485 779 Cuts at: 5420 5588 5856 928 Size: 168 268 3710 Fragments arranged by size: 3710 1369 971 779 651 485 268 171 168 48 18 BbvCI CC'TCA_GC Cuts at: 2393 2393 Size: 8638 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6829 6863 1903 Size: 34 4892 34 3678 Fragments arranged by size: 4892 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6530 7740 6530 Size: 1210 7428 BclI T'GATC_A Cuts at: 1548 2563 4068 1548 Size: 1015 1505 6118 Fragments arranged by size: 6118 1505 1015 BfrBI ATG'CAT Cuts at: 3539 5595 3539 Size: 2056 6582 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7249 136 Size: 107 122 71 631 605 5577 1525 Fragments arranged by size: 5577 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5239 5856 8633 5239 Size: 617 2777 5244 Fragments arranged by size: 5244 2777 617 BlpI GC'TnA_GC Cuts at: 2180 4773 6055 2180 Size: 2593 1282 4763 Fragments arranged by size: 4763 2593 1282 Bme1580I G_kGCm'C Cuts at: 1790 2145 3883 4268 4889 6699 7855 1790 Size: 355 1738 385 621 1810 1156 2573 Fragments arranged by size: 2573 1810 1738 1156 621 385 355 BmgBI CAC'GTC Cuts at: 1318 2595 3890 5185 1318 Size: 1277 1295 1295 4771 Fragments arranged by size: 4771 1295 1295 1277 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4288 5560 6369 Size: 416 53 83 399 24 2871 1272 809 Cuts at: 6369 7327 442 Size: 958 1753 Fragments arranged by size: 2871 1753 1272 958 809 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8638 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2861 2902 3180 2861 Size: 41 278 8319 Fragments arranged by size: 8319 278 41 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2831 3063 3627 3918 4764 4979 5447 Size: 908 684 232 564 291 846 215 468 Cuts at: 5447 5708 7298 1239 Size: 261 1590 2579 Fragments arranged by size: 2579 1590 908 846 684 564 468 291 261 232 215 Bpu10I CC'TnA_GC Cuts at: 2393 3390 2393 Size: 997 7641 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5062 6763 7628 7926 5062 Size: 1701 865 298 5774 Fragments arranged by size: 5774 1701 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2876 3645 4719 7301 882 Size: 617 1377 769 1074 2582 2219 Fragments arranged by size: 2582 2219 1377 1074 769 617 BsaAI yAC'GTr Cuts at: 493 1204 6429 493 Size: 711 5225 2702 Fragments arranged by size: 5225 2702 711 BsaBI GATnn'nnATC Cuts at: 8632 8632 Size: 8638 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6445 6827 7462 275 Size: 53 83 186 936 4912 382 635 1451 Fragments arranged by size: 4912 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2702 3007 7071 7469 7743 7890 8301 2702 Size: 305 4064 398 274 147 411 3039 Fragments arranged by size: 4064 3039 411 398 305 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2220 2250 1178 Size: 30 165 30 817 30 7566 Fragments arranged by size: 7566 817 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2894 5021 1186 Size: 949 759 2127 4803 Fragments arranged by size: 4803 2127 949 759 BseYI C'CCAG_C Cuts at: 7841 8337 7841 Size: 496 8142 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2767 3870 4159 4577 1163 Size: 573 1031 1103 289 418 5224 Fragments arranged by size: 5224 1103 1031 573 418 289 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6088 6849 7877 1540 Size: 275 186 4087 761 1028 2301 Fragments arranged by size: 4087 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2145 2871 3883 4268 4779 4811 5496 5745 6699 Size: 726 1012 385 511 32 685 249 954 Cuts at: 6699 6784 7855 2145 Size: 85 1071 2928 Fragments arranged by size: 2928 1071 1012 954 726 685 511 385 249 85 32 BsmI GAATG_Cn' Cuts at: 2822 3539 5687 6149 6242 2822 Size: 717 2148 462 93 5218 Fragments arranged by size: 5218 2148 717 462 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2871 3883 4268 4779 4811 4889 Size: 399 355 726 1012 385 511 32 78 Cuts at: 4889 5496 5745 5957 6699 6784 7855 1391 Size: 607 249 212 742 85 1071 2174 Fragments arranged by size: 2174 1071 1012 742 726 607 511 399 385 355 249 212 85 78 32 BspEI T'CCGG_A Cuts at: 8301 8301 Size: 8638 BspHI T'CATG_A Cuts at: 2674 2892 6527 8257 2674 Size: 218 3635 1730 3055 Fragments arranged by size: 3635 3055 1730 218 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2659 2899 3892 4149 5993 844 Size: 1815 240 993 257 1844 3489 Fragments arranged by size: 3489 1844 1815 993 257 240 BsrBI CCG'CTC Cuts at: 1394 1823 2840 3164 4903 6525 1394 Size: 429 1017 324 1739 1622 3507 Fragments arranged by size: 3507 1739 1622 1017 429 324 BsrDI GCAATG_nn' Cuts at: 65 1248 2796 3553 4406 5020 7133 7307 65 Size: 1183 1548 757 853 614 2113 174 1396 Fragments arranged by size: 2113 1548 1396 1183 853 757 614 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5285 7282 7469 1700 Size: 110 3475 1997 187 2869 Fragments arranged by size: 3475 2869 1997 187 110 BsrGI T'GTAC_A Cuts at: 96 2979 3698 5867 96 Size: 2883 719 2169 2867 Fragments arranged by size: 2883 2867 2169 719 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8638 BssSI C'ACGA_G Cuts at: 2864 5495 6698 7710 2864 Size: 2631 1203 1012 3792 Fragments arranged by size: 3792 2631 1203 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2454 3518 4182 4272 4365 4585 4806 5691 Size: 1660 1064 664 90 93 220 221 885 Cuts at: 5691 794 Size: 3741 Fragments arranged by size: 3741 1660 1064 885 664 221 220 93 90 BstBI TT'CG_AA Cuts at: 4509 5751 7442 4509 Size: 1242 1691 5705 Fragments arranged by size: 5705 1691 1242 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8638 BstYI r'GATC_y Cuts at: 1972 2033 2574 5239 5856 5968 6335 6720 6737 Size: 61 541 2665 617 112 367 385 17 Cuts at: 6737 8189 8275 8287 8633 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2665 1977 1452 617 541 385 367 346 112 86 61 17 12 BtgI C'CryG_G Cuts at: 513 1065 2305 2629 3111 5116 5839 513 Size: 552 1240 324 482 2005 723 3312 Fragments arranged by size: 3312 2005 1240 723 552 482 324 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7899 Fragments arranged by size: 7899 683 56 BtsI GCAGTG_nn' Cuts at: 2527 2539 5941 6154 6948 6968 2527 Size: 12 3402 213 794 20 4197 Fragments arranged by size: 4197 3402 794 213 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8638 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3096 3131 529 Size: 35 2532 35 6036 Fragments arranged by size: 6036 2532 35 35 DraI TTT'AAA Cuts at: 5942 5942 Size: 8638 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2593 4205 7645 809 Size: 507 1277 1612 3440 1802 Fragments arranged by size: 3440 1802 1612 1277 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2261 3834 6085 Size: 54 1425 50 275 18 431 1573 2251 Cuts at: 6085 6974 8603 8 Size: 889 1629 43 Fragments arranged by size: 2251 1629 1573 1425 889 431 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6085 1537 Size: 275 4273 4090 Fragments arranged by size: 4273 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2096 2876 3149 3483 3585 3586 4998 6568 Size: 743 780 273 334 102 1 1412 1570 Cuts at: 6568 1353 Size: 3423 Fragments arranged by size: 3423 1570 1412 780 743 334 273 102 1 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7225 7599 7745 387 Size: 1064 138 5636 374 146 1280 Fragments arranged by size: 5636 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2869 4777 5494 2869 Size: 1908 717 6013 Fragments arranged by size: 6013 1908 717 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2147 2831 3063 3464 3627 3918 Size: 393 462 53 684 232 401 163 291 Cuts at: 3918 4064 4115 4764 4979 5432 5447 5708 6701 Size: 146 51 649 215 453 15 261 993 Cuts at: 6701 7298 8085 8353 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 993 787 684 649 597 462 453 401 393 291 268 261 232 215 163 146 53 51 15 EcoNI CCTnn'n_nnAGG Cuts at: 4184 4184 Size: 8638 EcoO109I rG'GnC_Cy Cuts at: 3337 3394 3810 4865 3337 Size: 57 416 1055 7110 Fragments arranged by size: 7110 1055 416 57 EcoRI G'AATT_C Cuts at: 4019 5047 5222 5947 6325 4019 Size: 1028 175 725 378 6332 Fragments arranged by size: 6332 1028 725 378 175 EcoRV GAT'ATC Cuts at: 2188 5257 2188 Size: 3069 5569 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2596 5908 2596 Size: 3312 5326 FspI TGC'GCA Cuts at: 5206 7144 5206 Size: 1938 6700 FspAI rTGC'GCAy Cuts at: 5206 5206 Size: 8638 HaeII r_GCGC'y Cuts at: 1678 5313 7785 1678 Size: 3635 2472 2531 Fragments arranged by size: 3635 2531 2472 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3833 3865 3891 3923 3988 Size: 32 135 32 2261 32 26 32 65 Cuts at: 3988 4020 7279 7311 7353 7385 1373 Size: 32 3259 32 42 32 2626 Fragments arranged by size: 3259 2626 2261 135 65 42 32 32 32 32 32 32 32 26 HincII GTy'rAC Cuts at: 669 1657 1938 2216 3463 4642 5982 6228 669 Size: 988 281 278 1247 1179 1340 246 3079 Fragments arranged by size: 3079 1340 1247 1179 988 281 278 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8638 HpaI GTT'AAC Cuts at: 6228 6228 Size: 8638 KpnI G_GTAC'C Cuts at: 5963 5963 Size: 8638 MfeI C'AATT_G Cuts at: 6237 6237 Size: 8638 MluI A'CGCG_T Cuts at: 8514 8514 Size: 8638 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2106 2273 2622 3111 5550 7752 7936 1305 Size: 801 167 349 489 2439 2202 184 2007 Fragments arranged by size: 2439 2202 2007 801 489 349 184 167 MscI TGG'CCA Cuts at: 10 64 1489 1832 2263 3836 10 Size: 54 1425 343 431 1573 4812 Fragments arranged by size: 4812 1573 1425 431 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2148 2359 2532 2611 4118 4388 5934 Size: 648 982 211 173 79 1507 270 1546 Cuts at: 5934 6596 6955 7114 518 Size: 662 359 159 2042 Fragments arranged by size: 2042 1546 1507 982 662 648 359 270 211 173 159 79 MspA1I CmG'CkG Cuts at: 1606 2148 2157 3222 6054 6731 7879 8124 1606 Size: 542 9 1065 2832 677 1148 245 2120 Fragments arranged by size: 2832 2120 1148 1065 677 542 245 9 NaeI GCC'GGC Cuts at: 5287 5287 Size: 8638 NcoI C'CATG_G Cuts at: 513 1065 2305 3111 5116 5839 513 Size: 552 1240 806 2005 723 3312 Fragments arranged by size: 3312 2005 1240 806 723 552 NdeI CA'TA_TG Cuts at: 387 4848 5272 387 Size: 4461 424 3753 Fragments arranged by size: 4461 3753 424 NgoMIV G'CCGG_C Cuts at: 5285 5285 Size: 8638 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8638 NotI GC'GGCC_GC Cuts at: 6085 6085 Size: 8638 NsiI A_TGCA'T Cuts at: 3541 5597 3541 Size: 2056 6582 NspI r_CATG'y Cuts at: 1132 1229 2668 4052 4153 4885 5083 5134 5996 Size: 97 1439 1384 101 732 198 51 862 Cuts at: 5996 7541 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1439 1384 862 732 198 101 97 51 PasI CC'CwG_GG Cuts at: 1348 2345 1348 Size: 997 7641 PciI A'CATG_T Cuts at: 4881 7537 4881 Size: 2656 5982 PflMI CCAn_nnn'nTGG Cuts at: 1108 3650 1108 Size: 2542 6096 PfoI T'CCnGG_A Cuts at: 2708 2775 4839 2708 Size: 67 2064 6507 Fragments arranged by size: 6507 2064 67 PmeI GTTT'AAAC Cuts at: 5942 5942 Size: 8638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6707 6739 8220 8252 6707 Size: 32 1481 32 7093 Fragments arranged by size: 7093 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3337 3394 3337 Size: 57 8581 PshAI GACnn'nnGTC Cuts at: 1613 3399 1613 Size: 1786 6852 PsiI TTA'TAA Cuts at: 6208 6208 Size: 8638 PspXI vC'TCGA_Gb Cuts at: 3193 3193 Size: 8638 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3433 3465 3433 Size: 32 8606 PstI C_TGCA'G Cuts at: 830 2459 2534 3270 3523 4581 4710 5158 5696 Size: 1629 75 736 253 1058 129 448 538 Cuts at: 5696 5910 5990 830 Size: 214 80 3478 Fragments arranged by size: 3478 1629 1058 736 538 448 253 214 129 80 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8638 PvuII CAG'CTG Cuts at: 2148 2157 3222 2148 Size: 9 1065 7564 Fragments arranged by size: 7564 1065 9 SacI G_AGCT'C Cuts at: 2871 4779 5496 2871 Size: 1908 717 6013 Fragments arranged by size: 6013 1908 717 SalI G'TCGA_C Cuts at: 1936 5980 1936 Size: 4044 4594 SapI GCTCTTCn'nnn_ Cuts at: 2876 3585 2876 Size: 709 7929 SbfI CC_TGCA'GG Cuts at: 5990 5990 Size: 8638 ScaI AGT'ACT Cuts at: 6886 8598 6886 Size: 1712 6926 SfcI C'TryA_G Cuts at: 826 1045 2455 2530 3217 3266 3519 4258 4444 Size: 219 1410 75 687 49 253 739 186 Cuts at: 4444 4577 4706 5154 5692 5906 5986 7121 7802 Size: 133 129 448 538 214 80 1135 681 Cuts at: 7802 7993 826 Size: 191 1471 Fragments arranged by size: 1471 1410 1135 739 687 681 538 448 253 219 214 191 186 133 129 80 75 49 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8638 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8638 SmaI CCC'GGG Cuts at: 1872 5965 1872 Size: 4093 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3193 3371 3814 3854 5041 6742 Size: 197 926 1250 178 443 40 1187 1701 Cuts at: 6742 7643 7905 820 Size: 901 262 1553 Fragments arranged by size: 1701 1553 1250 1187 926 901 443 262 197 178 40 SnaBI TAC'GTA Cuts at: 493 6429 493 Size: 5936 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8638 SphI G_CATG'C Cuts at: 1132 5083 5996 1132 Size: 3951 913 3774 Fragments arranged by size: 3951 3774 913 SspI AAT'ATT Cuts at: 5 52 3714 6562 5 Size: 47 3662 2848 2081 Fragments arranged by size: 3662 2848 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8638 StyI C'CwwG_G Cuts at: 513 1065 2305 3111 3771 4339 4861 5100 5116 Size: 552 1240 806 660 568 522 239 16 Cuts at: 5116 5839 6077 513 Size: 723 238 3074 Fragments arranged by size: 3074 1240 806 723 660 568 552 522 239 238 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2850 4537 6665 6682 6835 7020 7465 Size: 1255 1270 1687 2128 17 153 185 445 Cuts at: 7465 325 Size: 1498 Fragments arranged by size: 2128 1687 1498 1270 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2979 3698 3930 Size: 274 80 33 51 1443 1002 719 232 Cuts at: 3930 4428 5867 6884 8596 96 Size: 498 1439 1017 1712 138 Fragments arranged by size: 1712 1443 1439 1017 1002 719 498 274 232 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3970 4002 8598 8630 3970 Size: 32 4596 32 3978 Fragments arranged by size: 4596 3978 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8638 XbaI T'CTAG_A Cuts at: 5974 5974 Size: 8638 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3094 5239 3094 Size: 2145 6493 XhoI C'TCGA_G Cuts at: 1943 3193 1943 Size: 1250 7388 XmaI C'CCGG_G Cuts at: 1870 5963 1870 Size: 4093 4545 XmnI GAAnn'nnTTC Cuts at: 5888 6767 5888 Size: 879 7759 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6446 276 Size: 53 83 186 936 4912 2468 Fragments arranged by size: 4912 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FseI KasI NarI NruI PacI PmlI PspOMI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI