(Circular) (Six-base) MAPSORT of: pFN21ASDF0128.seq Check: 1085 from: 1 to: 9286 pFN21ASDF0128 9286 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2202 2335 4384 2202 Size: 133 2049 7104 Fragments arranged by size: 7104 2049 133 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6393 7096 278 Size: 53 83 186 936 4857 703 2468 Fragments arranged by size: 4857 2468 936 703 186 83 53 AccI GT'mk_AC Cuts at: 1937 5166 6629 1937 Size: 3229 1463 4594 Fragments arranged by size: 4594 3229 1463 Acc65I G'GTAC_C Cuts at: 2179 2690 2833 3131 6607 2179 Size: 511 143 298 3476 4858 Fragments arranged by size: 4858 3476 511 298 143 AclI AA'CG_TT Cuts at: 1563 7413 7786 1563 Size: 5850 373 3063 Fragments arranged by size: 5850 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2820 3138 3420 3839 5441 5840 5961 7349 Size: 1188 318 282 419 1602 399 121 1388 Cuts at: 7349 8733 9001 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1602 1388 1384 1188 419 399 318 282 268 121 AfeI AGC'GCT Cuts at: 4507 4649 4507 Size: 142 9144 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9089 AflIII A'CryG_T Cuts at: 1203 5191 8185 9162 1203 Size: 3988 2994 977 1327 Fragments arranged by size: 3988 2994 1327 977 AgeI A'CCGG_T Cuts at: 8117 8117 Size: 9286 AhdI GACnn_n'nnGTC Cuts at: 4462 8015 4462 Size: 3553 5733 AleI CACnn'nnGTG Cuts at: 1166 2386 2464 5159 1166 Size: 1220 78 2695 5293 Fragments arranged by size: 5293 2695 1220 78 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2214 5795 1184 Size: 1030 3581 4675 Fragments arranged by size: 4675 3581 1030 AlwNI CAG_nnn'CTG Cuts at: 2514 2548 2798 2987 3041 3350 3778 3889 4038 Size: 34 250 189 54 309 428 111 149 Cuts at: 4038 4378 4603 4706 5287 5644 6133 7029 8601 Size: 340 225 103 581 357 489 896 1572 Cuts at: 8601 2514 Size: 3199 Fragments arranged by size: 3199 1572 896 581 489 428 357 340 309 250 225 189 149 111 103 54 34 ApaI G_GGCC'C Cuts at: 4418 4636 4756 4795 4957 4968 5084 5728 5939 Size: 218 120 39 162 11 116 644 211 Cuts at: 5939 4418 Size: 7765 Fragments arranged by size: 7765 644 218 211 162 120 116 39 11 ApaLI G'TGCA_C Cuts at: 2582 6057 7343 8499 2582 Size: 3475 1286 1156 3369 Fragments arranged by size: 3475 3369 1286 1156 ApoI r'AATT_y Cuts at: 1493 6399 6595 6821 6973 9082 1493 Size: 4906 196 226 152 2109 1697 Fragments arranged by size: 4906 2109 1697 226 196 152 AseI AT'TA_AT Cuts at: 160 1032 7840 160 Size: 872 6808 1606 Fragments arranged by size: 6808 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9286 AvaI C'yCGr_G Cuts at: 1870 1943 2913 3714 4411 5409 5625 6276 6549 Size: 73 970 801 697 998 216 651 273 Cuts at: 6549 6611 1870 Size: 62 4545 Fragments arranged by size: 4545 998 970 801 697 651 273 216 73 62 BamHI G'GATC_C Cuts at: 4875 6616 6983 4875 Size: 1741 367 7178 Fragments arranged by size: 7178 1741 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2179 2350 2690 2833 3131 Size: 325 217 627 392 171 340 143 298 Cuts at: 3131 3811 4772 4937 5240 5384 6607 8062 618 Size: 680 961 165 303 144 1223 1455 1842 Fragments arranged by size: 1842 1455 1223 961 680 627 392 340 325 303 298 217 171 165 144 143 BanII G_rGCy'C Cuts at: 1391 2565 2789 3030 3193 3227 3366 3667 3705 Size: 1174 224 241 163 34 139 301 38 Cuts at: 3705 4100 4418 4636 4756 4764 4795 4907 4957 Size: 395 318 218 120 8 31 112 50 Cuts at: 4957 4968 5084 5600 5632 5685 5709 5728 5939 Size: 11 116 516 32 53 24 19 211 Cuts at: 5939 6165 6556 6605 1391 Size: 226 391 49 4072 Fragments arranged by size: 4072 1174 516 395 391 318 301 241 226 224 218 211 163 139 120 116 112 53 50 49 38 34 32 31 24 19 11 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 8315 BbvCI CC'TCA_GC Cuts at: 2356 2988 3390 3945 4509 4988 2356 Size: 632 402 555 564 479 6654 Fragments arranged by size: 6654 632 564 555 479 402 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5862 5896 7477 7511 1903 Size: 34 3925 34 1581 34 3678 Fragments arranged by size: 3925 3678 1581 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2085 7178 8388 2085 Size: 5093 1210 2983 Fragments arranged by size: 5093 2983 1210 BclI T'GATC_A Cuts at: 1548 3586 1548 Size: 2038 7248 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2940 3489 4911 Size: 107 122 71 631 605 1268 549 1422 Cuts at: 4911 5732 5792 6287 7897 136 Size: 821 60 495 1610 1525 Fragments arranged by size: 1610 1525 1422 1268 821 631 605 549 495 122 107 71 60 BglII A'GATC_T Cuts at: 5815 9281 5815 Size: 3466 5820 BlpI GC'TnA_GC Cuts at: 2559 6703 2559 Size: 4144 5142 Bme1580I G_kGCm'C Cuts at: 1790 2227 2355 2433 2586 2715 3217 3692 4418 Size: 437 128 78 153 129 502 475 726 Cuts at: 4418 4636 4756 4795 4957 4968 5084 5278 5387 Size: 218 120 39 162 11 116 194 109 Cuts at: 5387 5410 5728 5939 6061 6250 7347 8503 1790 Size: 23 318 211 122 189 1097 1156 2573 Fragments arranged by size: 2573 1156 1097 726 502 475 437 318 218 211 194 189 162 153 129 128 122 120 116 109 78 39 23 11 BmgBI CAC'GTC Cuts at: 1318 5175 1318 Size: 3857 5429 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3788 4026 4048 Size: 416 53 83 399 24 2371 238 22 Cuts at: 4048 4758 4951 7017 7975 442 Size: 710 193 2066 958 1753 Fragments arranged by size: 2371 2066 1753 958 710 416 399 238 193 83 53 24 22 BmtI G_CTAG'C Cuts at: 1056 2300 1056 Size: 1244 8042 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6513 6564 6513 Size: 51 9235 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2634 2803 2874 3464 3542 3810 4145 4292 Size: 1395 169 71 590 78 268 335 147 Cuts at: 4292 4373 4574 4625 5079 5162 5579 5696 6453 Size: 81 201 51 454 83 417 117 757 Cuts at: 6453 7946 1239 Size: 1493 2579 Fragments arranged by size: 2579 1493 1395 757 590 454 417 335 268 201 169 147 117 83 81 78 71 51 Bpu10I CC'TnA_GC Cuts at: 2031 2356 2988 3334 3390 3758 3945 4509 4988 Size: 325 632 346 56 368 187 564 479 Cuts at: 4988 5085 5361 5703 2031 Size: 97 276 342 5614 Fragments arranged by size: 5614 632 564 479 368 346 342 325 276 187 97 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2369 3865 5353 5572 5915 6089 7411 8276 8574 Size: 1496 1488 219 343 174 1322 865 298 Cuts at: 8574 2369 Size: 3081 Fragments arranged by size: 3081 1496 1488 1322 865 343 298 219 174 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5769 5841 7949 882 Size: 617 4270 72 2108 2219 Fragments arranged by size: 4270 2219 2108 617 72 BsaAI yAC'GTr Cuts at: 493 1204 4351 5194 5323 5539 6583 7077 493 Size: 711 3147 843 129 216 1044 494 2702 Fragments arranged by size: 3147 2702 1044 843 711 494 216 129 BsaBI GATnn'nnATC Cuts at: 9280 9280 Size: 9286 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5369 6390 7093 7475 Size: 53 83 186 936 3836 1021 703 382 Cuts at: 7475 8110 275 Size: 635 1451 Fragments arranged by size: 3836 1451 1021 936 703 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2826 2871 2957 3056 5433 7719 8117 8391 8538 Size: 45 86 99 2377 2286 398 274 147 Cuts at: 8538 8949 2826 Size: 411 3163 Fragments arranged by size: 3163 2377 2286 411 398 274 147 99 86 45 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3983 4013 5733 5763 1178 Size: 30 165 30 2580 30 1720 30 4701 Fragments arranged by size: 4701 2580 1720 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2693 3105 3108 3111 3114 3117 3216 3713 Size: 1507 412 3 3 3 3 99 497 Cuts at: 3713 4004 4133 4166 4439 4571 4885 4910 5693 Size: 291 129 33 273 132 314 25 783 Cuts at: 5693 5903 6383 6386 1186 Size: 210 480 3 4086 Fragments arranged by size: 4086 1507 783 497 480 412 314 291 273 210 132 129 99 33 25 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2660 2686 3031 4093 4259 4285 4706 4816 4982 Size: 26 345 1062 166 26 421 110 166 Cuts at: 4982 5006 5200 5277 5696 6126 6464 8489 8985 Size: 24 194 77 419 430 338 2025 496 Cuts at: 8985 2660 Size: 2961 Fragments arranged by size: 2961 2025 1062 496 430 421 419 345 338 194 166 166 110 77 26 26 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2072 2312 2435 3551 4331 4373 4719 Size: 573 336 240 123 1116 780 42 346 Cuts at: 4719 5559 6191 1163 Size: 840 632 4258 Fragments arranged by size: 4258 1116 840 780 632 573 346 336 240 123 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6736 7497 8525 1540 Size: 275 186 4735 761 1028 2301 Fragments arranged by size: 4735 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2060 2565 2586 2932 3291 3366 3667 5180 5685 Size: 505 21 346 359 75 301 1513 505 Cuts at: 5685 5709 6061 6165 7347 7432 8503 2060 Size: 24 352 104 1182 85 1071 2843 Fragments arranged by size: 2843 1513 1182 1071 505 505 359 352 346 301 104 85 75 24 21 BsmI GAATG_Cn' Cuts at: 3308 4170 4567 6797 6890 3308 Size: 862 397 2230 93 5704 Fragments arranged by size: 5704 2230 862 397 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3586 3586 Size: 9286 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2227 2355 2433 2565 2586 2715 Size: 399 270 167 128 78 132 21 129 Cuts at: 2715 2789 2932 3030 3193 3217 3227 3291 3366 Size: 74 143 98 163 24 10 64 75 Cuts at: 3366 3667 3692 3705 4100 4418 4636 4756 4764 Size: 301 25 13 395 318 218 120 8 Cuts at: 4764 4795 4907 4957 4968 5084 5180 5278 5387 Size: 31 112 50 11 116 96 98 109 Cuts at: 5387 5410 5600 5632 5685 5709 5728 5939 6061 Size: 23 190 32 53 24 19 211 122 Cuts at: 6061 6165 6250 6556 6605 7347 7432 8503 1391 Size: 104 85 306 49 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 395 318 306 301 270 218 211 190 167 163 143 132 129 128 122 120 116 112 109 104 98 98 96 85 85 78 75 74 64 53 50 49 32 31 25 24 24 23 21 19 13 11 10 8 BspEI T'CCGG_A Cuts at: 2826 3056 8949 2826 Size: 230 5893 3163 Fragments arranged by size: 5893 3163 230 BspHI T'CATG_A Cuts at: 3904 7175 8905 3904 Size: 3271 1730 4285 Fragments arranged by size: 4285 3271 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2202 2335 2522 3170 4353 4384 4600 5628 Size: 1358 133 187 648 1183 31 216 1028 Cuts at: 5628 5787 6641 844 Size: 159 854 3489 Fragments arranged by size: 3489 1358 1183 1028 854 648 216 187 159 133 31 BsrBI CCG'CTC Cuts at: 1394 1823 2266 6235 7173 1394 Size: 429 443 3969 938 3507 Fragments arranged by size: 3969 3507 938 443 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5038 7781 7955 65 Size: 1183 3790 2743 174 1396 Fragments arranged by size: 3790 2743 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2490 4769 6311 7930 8117 1700 Size: 110 680 2279 1542 1619 187 2869 Fragments arranged by size: 2869 2279 1619 1542 680 187 110 BsrGI T'GTAC_A Cuts at: 96 5740 96 Size: 5644 3642 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9286 BssSI C'ACGA_G Cuts at: 6060 7346 8358 6060 Size: 1286 1012 6988 Fragments arranged by size: 6988 1286 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2320 2350 4186 4378 4399 4442 4937 794 Size: 1526 30 1836 192 21 43 495 5143 Fragments arranged by size: 5143 1836 1526 495 192 43 30 21 BstBI TT'CG_AA Cuts at: 2007 8090 2007 Size: 6083 3203 BstEII G'GTnAC_C Cuts at: 1184 5146 1184 Size: 3962 5324 BstXI CCAn_nnnn'nTGG Cuts at: 2107 2234 2107 Size: 127 9159 BstYI r'GATC_y Cuts at: 1972 3059 3868 4195 4875 5815 6382 6519 6616 Size: 1087 809 327 680 940 567 137 97 Cuts at: 6616 6983 7368 7385 8837 8923 8935 9281 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1087 940 809 680 567 385 367 346 327 137 97 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2676 2736 3071 3696 5687 2676 Size: 60 335 625 1991 6275 Fragments arranged by size: 6275 1991 625 335 60 BtgI C'CryG_G Cuts at: 513 1065 2022 4907 5021 5183 5506 5601 5767 Size: 552 957 2885 114 162 323 95 166 Cuts at: 5767 5787 6186 6285 513 Size: 20 399 99 3514 Fragments arranged by size: 3514 2885 957 552 399 323 166 162 114 99 95 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8547 Fragments arranged by size: 8547 683 56 BtsI GCAGTG_nn' Cuts at: 2621 3752 3818 4004 4164 4175 4211 4379 4409 Size: 1131 66 186 160 11 36 168 30 Cuts at: 4409 4951 6802 7596 7616 2621 Size: 542 1851 794 20 4291 Fragments arranged by size: 4291 1851 1131 794 542 186 168 160 66 36 30 20 11 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9286 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9251 DraI TTT'AAA Cuts at: 6590 6590 Size: 9286 DraIII CAC_nnn'GTG Cuts at: 3284 3284 Size: 9286 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8293 809 Size: 507 6977 1802 Fragments arranged by size: 6977 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2261 2492 3978 Size: 54 1425 50 275 18 431 231 1486 Cuts at: 3978 5024 5325 5784 5869 6288 6507 6733 7622 Size: 1046 301 459 85 419 219 226 889 Cuts at: 7622 9251 8 Size: 1629 43 Fragments arranged by size: 1629 1486 1425 1046 889 459 431 419 301 275 231 226 219 85 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6733 1537 Size: 275 4921 4090 Fragments arranged by size: 4921 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2249 3414 3555 3741 4143 5070 5906 6256 Size: 896 1165 141 186 402 927 836 350 Cuts at: 6256 6433 7216 1353 Size: 177 783 3423 Fragments arranged by size: 3423 1165 927 896 836 783 402 350 186 177 141 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7873 8247 8393 387 Size: 1064 138 6284 374 146 1280 Fragments arranged by size: 6284 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2563 3364 3665 5683 5707 6163 2563 Size: 801 301 2018 24 456 5686 Fragments arranged by size: 5686 2018 801 456 301 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2634 2803 2820 2874 3138 3420 3464 Size: 393 1002 169 17 54 264 282 44 Cuts at: 3464 3542 3810 3839 4145 4292 4373 4574 4625 Size: 78 268 29 306 147 81 201 51 Cuts at: 4625 5079 5162 5441 5579 5696 5840 5961 6453 Size: 454 83 279 138 117 144 121 492 Cuts at: 6453 7349 7946 8733 9001 1239 Size: 896 597 787 268 1524 Fragments arranged by size: 1524 1002 896 787 597 492 454 393 306 282 279 268 268 264 201 169 147 144 138 121 117 83 81 78 54 51 44 29 17 EcoNI CCTnn'n_nnAGG Cuts at: 2727 3314 3806 4529 4892 2727 Size: 587 492 723 363 7121 Fragments arranged by size: 7121 723 587 492 363 EcoO109I rG'GnC_Cy Cuts at: 2593 2732 2902 2909 3491 3565 4021 4543 4633 Size: 139 170 7 582 74 456 522 90 Cuts at: 4633 4753 4792 4953 4964 4965 5080 5081 5112 Size: 120 39 161 11 1 115 1 31 Cuts at: 5112 5490 5725 5935 5936 2593 Size: 378 235 210 1 5943 Fragments arranged by size: 5943 582 522 456 378 235 210 170 161 139 120 115 90 74 39 31 11 7 1 1 1 EcoRI G'AATT_C Cuts at: 6595 6973 6595 Size: 378 8908 EcoRV GAT'ATC Cuts at: 4588 4588 Size: 9286 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3278 3584 5992 3278 Size: 306 2408 6572 Fragments arranged by size: 6572 2408 306 FspI TGC'GCA Cuts at: 7792 7792 Size: 9286 HaeII r_GCGC'y Cuts at: 1678 2687 2853 4509 4525 4651 8433 1678 Size: 1009 166 1656 16 126 3782 2531 Fragments arranged by size: 3782 2531 1656 1009 166 126 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2125 2157 2367 2399 3615 Size: 32 135 32 553 32 210 32 1216 Cuts at: 3615 3647 3938 3970 4448 4480 7927 7959 8001 Size: 32 291 32 478 32 3447 32 42 Cuts at: 8001 8033 1373 Size: 32 2626 Fragments arranged by size: 3447 2626 1216 553 478 291 210 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6630 6876 669 Size: 988 281 4692 246 3079 Fragments arranged by size: 4692 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3293 4438 748 Size: 2545 1145 5596 Fragments arranged by size: 5596 2545 1145 HpaI GTT'AAC Cuts at: 6876 6876 Size: 9286 KpnI G_GTAC'C Cuts at: 2183 2694 2837 3135 6611 2183 Size: 511 143 298 3476 4858 Fragments arranged by size: 4858 3476 511 298 143 MfeI C'AATT_G Cuts at: 6885 6885 Size: 9286 MluI A'CGCG_T Cuts at: 9162 9162 Size: 9286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2584 3533 3941 4365 8400 8584 1305 Size: 1279 949 408 424 4035 184 2007 Fragments arranged by size: 4035 2007 1279 949 424 408 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3980 5327 6509 10 Size: 54 1425 343 2148 1347 1182 2787 Fragments arranged by size: 2787 2148 1425 1347 1182 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2386 2464 3594 4238 4669 5159 7244 Size: 648 1220 78 1130 644 431 490 2085 Cuts at: 7244 7603 7762 518 Size: 359 159 2042 Fragments arranged by size: 2085 2042 1220 1130 648 644 490 431 359 159 78 MspA1I CmG'CkG Cuts at: 1606 2360 2545 3520 3886 4369 4432 4600 4842 Size: 754 185 975 366 483 63 168 242 Cuts at: 4842 4854 5284 5653 5746 6021 6130 6702 7379 Size: 12 430 369 93 275 109 572 677 Cuts at: 7379 8527 8772 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 975 754 677 572 483 430 369 366 275 245 242 185 168 109 93 63 12 NaeI GCC'GGC Cuts at: 4771 6313 4771 Size: 1542 7744 NcoI C'CATG_G Cuts at: 513 1065 2022 5601 6186 513 Size: 552 957 3579 585 3613 Fragments arranged by size: 3613 3579 957 585 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9286 NgoMIV G'CCGG_C Cuts at: 4769 6311 4769 Size: 1542 7744 NheI G'CTAG_C Cuts at: 1052 2296 1052 Size: 1244 8042 NotI GC'GGCC_GC Cuts at: 6733 6733 Size: 9286 NspI r_CATG'y Cuts at: 1132 1229 6311 6644 8189 1132 Size: 97 5082 333 1545 2229 Fragments arranged by size: 5082 2229 1545 333 97 PasI CC'CwG_GG Cuts at: 1348 2399 2553 3227 4418 5015 5229 5354 6485 Size: 1051 154 674 1191 597 214 125 1131 Cuts at: 6485 1348 Size: 4149 Fragments arranged by size: 4149 1191 1131 1051 674 597 214 154 125 PciI A'CATG_T Cuts at: 8185 8185 Size: 9286 PflMI CCAn_nnn'nTGG Cuts at: 1108 2128 4038 4060 5354 1108 Size: 1020 1910 22 1294 5040 Fragments arranged by size: 5040 1910 1294 1020 22 PfoI T'CCnGG_A Cuts at: 4515 4779 5673 5751 6210 4515 Size: 264 894 78 459 7591 Fragments arranged by size: 7591 894 459 264 78 PmeI GTTT'AAAC Cuts at: 6590 6590 Size: 9286 PmlI CAC'GTG Cuts at: 4351 5194 4351 Size: 843 8443 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7355 7387 8868 8900 7355 Size: 32 1481 32 7741 Fragments arranged by size: 7741 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2732 4021 2732 Size: 1289 7997 PshAI GACnn'nnGTC Cuts at: 1613 3804 1613 Size: 2191 7095 PsiI TTA'TAA Cuts at: 6856 6856 Size: 9286 PspOMI G'GGCC_C Cuts at: 4414 4632 4752 4791 4953 4964 5080 5724 5935 Size: 218 120 39 162 11 116 644 211 Cuts at: 5935 4414 Size: 7765 Fragments arranged by size: 7765 644 218 211 162 120 116 39 11 PspXI vC'TCGA_Gb Cuts at: 6549 6549 Size: 9286 PstI C_TGCA'G Cuts at: 830 2346 3181 3999 4182 4218 4476 5637 6081 Size: 1516 835 818 183 36 258 1161 444 Cuts at: 6081 6638 830 Size: 557 3478 Fragments arranged by size: 3478 1516 1161 835 818 557 444 258 183 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9286 PvuII CAG'CTG Cuts at: 2360 4432 5653 5746 6130 2360 Size: 2072 1221 93 384 5516 Fragments arranged by size: 5516 2072 1221 384 93 SacI G_AGCT'C Cuts at: 2565 3366 3667 5685 5709 6165 2565 Size: 801 301 2018 24 456 5686 Fragments arranged by size: 5686 2018 801 456 301 24 SalI G'TCGA_C Cuts at: 1936 6628 1936 Size: 4692 4594 SanDI GG'GwC_CC Cuts at: 4021 4021 Size: 9286 SapI GCTCTTCn'nnn_ Cuts at: 6256 6256 Size: 9286 SbfI CC_TGCA'GG Cuts at: 4476 6638 4476 Size: 2162 7124 ScaI AGT'ACT Cuts at: 7534 9246 7534 Size: 1712 7574 SexAI A'CCwGG_T Cuts at: 3127 3309 3127 Size: 182 9104 SfcI C'TryA_G Cuts at: 826 1045 2342 3177 3414 3770 3995 4178 4214 Size: 219 1297 835 237 356 225 183 36 Cuts at: 4214 4472 4643 5633 6077 6634 7769 8450 8641 Size: 258 171 990 444 557 1135 681 191 Cuts at: 8641 826 Size: 1471 Fragments arranged by size: 1471 1297 1135 990 835 681 557 444 356 258 237 225 219 191 183 171 36 SfiI GGCCn_nnn'nGGCC Cuts at: 3489 6287 3489 Size: 2798 6488 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9286 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9286 SmaI CCC'GGG Cuts at: 1872 5411 6278 6613 1872 Size: 3539 867 335 4545 Fragments arranged by size: 4545 3539 867 335 SmlI C'TyrA_G Cuts at: 820 1017 1943 2384 3844 5332 5587 5930 6104 Size: 197 926 441 1460 1488 255 343 174 Cuts at: 6104 6549 7390 8291 8553 820 Size: 445 841 901 262 1553 Fragments arranged by size: 1553 1488 1460 926 901 841 445 441 343 262 255 197 174 SnaBI TAC'GTA Cuts at: 493 6583 7077 493 Size: 6090 494 2702 Fragments arranged by size: 6090 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9286 SphI G_CATG'C Cuts at: 1132 6311 6644 1132 Size: 5179 333 3774 Fragments arranged by size: 5179 3774 333 SspI AAT'ATT Cuts at: 5 52 7210 5 Size: 47 7158 2081 Fragments arranged by size: 7158 2081 47 StuI AGG'CCT Cuts at: 1517 2814 3456 3763 5139 5975 6377 1517 Size: 1297 642 307 1376 836 402 4426 Fragments arranged by size: 4426 1376 1297 836 642 402 307 StyI C'CwwG_G Cuts at: 513 1065 2022 2202 3262 4084 4279 5601 5728 Size: 552 957 180 1060 822 195 1322 127 Cuts at: 5728 6186 6226 6725 513 Size: 458 40 499 3074 Fragments arranged by size: 3074 1322 1060 957 822 552 499 458 195 180 127 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4663 5486 7313 7330 7483 7668 8113 Size: 1255 3083 823 1827 17 153 185 445 Cuts at: 8113 325 Size: 1498 Fragments arranged by size: 3083 1827 1498 1255 823 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2618 5740 7532 Size: 274 80 33 51 1443 641 3122 1792 Cuts at: 7532 9244 96 Size: 1712 138 Fragments arranged by size: 3122 1792 1712 1443 641 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3983 4015 6229 6261 9246 9278 3983 Size: 32 2214 32 2985 32 3991 Fragments arranged by size: 3991 2985 2214 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6540 1316 Size: 5224 4062 XbaI T'CTAG_A Cuts at: 6622 6622 Size: 9286 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3260 4120 4267 5158 5226 3260 Size: 860 147 891 68 7320 Fragments arranged by size: 7320 891 860 147 68 XhoI C'TCGA_G Cuts at: 1943 6549 1943 Size: 4606 4680 XmaI C'CCGG_G Cuts at: 1870 5409 6276 6611 1870 Size: 3539 867 335 4545 Fragments arranged by size: 4545 3539 867 335 XmnI GAAnn'nnTTC Cuts at: 5418 7415 5418 Size: 1997 7289 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6391 7094 276 Size: 53 83 186 936 4857 703 2468 Fragments arranged by size: 4857 2468 936 703 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I FseI FspAI KasI NarI NruI NsiI PacI PsrI RsrII SacII SfoI SrfI SwaI