Restriction map for
pFN21ASDB3041
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AspEI AspI AviII BbeI
BbrPI BbvCI BclI BfrI BglII
BsaAI BsaBI BsaHI BsiEI BsiWI
Bsp1407I BspDI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstUI BstZ17I Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoRV
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI KspI MamI
MluI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 1111 GGTACC G^GTACC
AccI 715 GTAGAC GT^[AC][GT]AC
AccIII 2384 TCCGGA T^CCGGA
AflIII 3582 ACATGT A^C[GA][CT]GT
Aor51HI 1593 AGCGCT AGC^GCT
AseI 781 ATTAAT AT^TAAT
AsnI 781 ATTAAT AT^TAAT
Asp700I 1729 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1111 GGTACC G^GTACC
AvaI 2222 CTCGAG C^[CT]CG[GA]G
AvrII 346 CCTAGG C^CTAGG
BalI 3641 TGGCCA TGG^CCA
BanI 1111 GGTACC G^G[CT][GA]CC
BcgI 3113 GCATGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 106 CCCGG CC^[GC]GG
BlnI 346 CCTAGG C^CTAGG
BsaWI 2384 TCCGGA [AT]^CCGG[AT]
BseAI 2384 TCCGGA T^CCGGA
BsgI 3294 GTGCAG GTGCAG(16/14)
BsmI 3147 GCATTC GAATGC(1/-1)
BsoBI 2222 CTCGAG C^[CT]CG[GA]G
BspEI 2384 TCCGGA T^CCGGA
BspLU11I 3582 ACATGT A^CATGT
BstEII 3441 GGTGACC G^GT[ACGT]ACC
BstPI 3441 GGTGACC G^GT[ACGT]ACC
BstXI 2573 CCACCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 229 TTTAAA TTT^AAA
DraII 1610 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 3342 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1509 GACGAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 3641 TGGCCA [CT]^GGCC[GA]
Eco47III 1593 AGCGCT AGC^GCT
EcoO109I 1610 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3441 GGTGACC G^GT[ACGT]ACC
FauI 1938 GCGGG CCCGC(4/6)
HincII 876 GTTGAC GT[CT]^[GA]AC
HindII 876 GTTGAC GT[CT]^[GA]AC
KpnI 1111 GGTACC GGTAC^C
MfeI 1411 CAATTG C^AATTG
MluNI 3641 TGGCCA TGG^CCA
MroI 2384 TCCGGA T^CCGGA
MscI 3641 TGGCCA TGG^CCA
MunI 1411 CAATTG C^AATTG
NciI 106 CCCGG CC^[GC]GG
NcoI 1104 CCATGG C^CATGG
PaeR7I 2222 CTCGAG C^TCGAG
PshBI 781 ATTAAT AT^TAAT
SacI 1306 GAGCTC GAGCT^C
ScaI 2266 AGTACT AGT^ACT
StuI 795 AGGCCT AGG^CCT
XbaI 957 TCTAGA T^CTAGA
XcmI 2548 CCAAAACAACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2222 CTCGAG C^TCGAG
XmnI 1729 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BamHI 495 GGATCC G^GATCC
3429 GGATCC
BanII 1306 GAGCTC G[GA]GC[CT]^C
3635 GGGCTC
BciVI 555 GGATAC GTATCC(6/5)
3024 GGATAC
BglI 601 GCCTCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3359 GCCAGCAAGGC
BlpI 2752 GCTGAGC GC^T[ACGT]AGC
2877 GCTGAGC
Bpu1102I 2752 GCTGAGC GC^T[ACGT]AGC
2877 GCTGAGC
BsaI 2033 GAGACC GGTCTC(1/5)
2622 GGTCTC
BssSI 464 CTCGTG CACGAG(-5/-1)
3083 CTCGTG
CelII 2752 GCTGAGC GC^T[ACGT]AGC
2877 GCTGAGC
EcoRI 20 GAATTC G^AATTC
2482 GAATTC
HapII 107 CCGG C^CGG
2385 CCGG
HpaII 107 CCGG C^CGG
2385 CCGG
MaeII 1800 ACGT A^CGT
2103 ACGT
MspI 107 CCGG C^CGG
2385 CCGG
SapI 1303 GAAGAGC GCTCTTC(1/4)
363 GCTCTTC
SphI 1317 GCATGC GCATG^C
1542 GCATGC
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DsaI EarI
Eco81I EcoNI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HgaI HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII