Restriction map for
pFN21ASDB3041
(Circular) (Six-base) MAPSORT of: pFN21ASDB3041.seq Check: 6160 from: 1 to: 8425
pFN21ASDB3041 8425 bp
With 182 enzymes: SgfI *
January 7, 2013 17:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5536 5536
Size: 8425
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6235 278
Size: 53 83 186 936 4699 2468
Fragments arranged by size:
4699 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2739 5768 1937
Size: 802 3029 4594
Fragments arranged by size:
4594 3029 802
Acc65I G'GTAC_C
Cuts at: 3134 5746 3134
Size: 2612 5813
AclI AA'CG_TT
Cuts at: 1563 6552 6925 1563
Size: 4989 373 3063
Fragments arranged by size:
4989 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2799 3752 3878 4452 4931 6488 7872 8140
Size: 1167 953 126 574 479 1557 1384 268
Cuts at: 8140 1632
Size: 1917
Fragments arranged by size:
1917 1557 1384 1167 953 574 479 268
126
AfeI AGC'GCT
Cuts at: 3618 3618
Size: 8425
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8228
AflIII A'CryG_T
Cuts at: 1203 5605 7324 8301 1203
Size: 4402 1719 977 1327
Fragments arranged by size:
4402 1719 1327 977
AgeI A'CCGG_T
Cuts at: 7256 7256
Size: 8425
AhdI GACnn_n'nnGTC
Cuts at: 7154 7154
Size: 8425
AleI CACnn'nnGTG
Cuts at: 1166 4912 1166
Size: 3746 4679
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8425
AlwNI CAG_nnn'CTG
Cuts at: 3205 3835 4148 4543 4897 4900 5002 5551 6168
Size: 630 313 395 354 3 102 549 617
Cuts at: 6168 7740 3205
Size: 1572 3890
Fragments arranged by size:
3890 1572 630 617 549 395 354 313
102 3
ApaLI G'TGCA_C
Cuts at: 6482 7638 6482
Size: 1156 7269
ApoI r'AATT_y
Cuts at: 1493 2043 2420 2463 4505 5734 5960 6112 8221
Size: 550 377 43 2042 1229 226 152 2109
Cuts at: 8221 1493
Size: 1697
Fragments arranged by size:
2109 2042 1697 1229 550 377 226 152
43
AseI AT'TA_AT
Cuts at: 160 1032 2805 6979 160
Size: 872 1773 4174 1606
Fragments arranged by size:
4174 1773 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8425
AvaI C'yCGr_G
Cuts at: 1870 1943 4245 5750 1870
Size: 73 2302 1505 4545
Fragments arranged by size:
4545 2302 1505 73
AvrII C'CTAG_G
Cuts at: 2369 2369
Size: 8425
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3480 3513 3480
Size: 33 8392
BamHI G'GATC_C
Cuts at: 2518 5452 5755 6122 2518
Size: 2934 303 367 4821
Fragments arranged by size:
4821 2934 367 303
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3134 5746 7201 618
Size: 325 217 627 1347 2612 1455 1842
Fragments arranged by size:
2612 1842 1455 1347 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3333 5662 5744 1391
Size: 1942 2329 82 4072
Fragments arranged by size:
4072 2329 1942 82
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2206 2564 3393 3534 4569 5567 928
Size: 971 307 358 829 141 1035 998 3786
Fragments arranged by size:
3786 1035 998 971 829 358 307 141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5125 5159 6616 6650 1903
Size: 34 3188 34 1457 34 3678
Fragments arranged by size:
3678 3188 1457 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2572 5041 6317 7527 2572
Size: 2469 1276 1210 3470
Fragments arranged by size:
3470 2469 1276 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8425
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2630 5388 7036
Size: 107 122 71 631 605 958 2758 1648
Cuts at: 7036 136
Size: 1525
Fragments arranged by size:
2758 1648 1525 958 631 605 122 107
71
BglII A'GATC_T
Cuts at: 8420 8420
Size: 8425
BlpI GC'TnA_GC
Cuts at: 4776 4901 5842 4776
Size: 125 941 7359
Fragments arranged by size:
7359 941 125
Bme1580I G_kGCm'C
Cuts at: 1790 2130 2883 4621 5408 6486 7642 1790
Size: 340 753 1738 787 1078 1156 2573
Fragments arranged by size:
2573 1738 1156 1078 787 753 340
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8425
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2638 3439 6156
Size: 416 53 83 399 24 1221 801 2717
Cuts at: 6156 7114 442
Size: 958 1753
Fragments arranged by size:
2717 1753 1221 958 801 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8425
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4663 4663
Size: 8425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3812 3857 4160 4997 5046 5147 5468 5622
Size: 2573 45 303 837 49 101 321 154
Cuts at: 5622 7085 1239
Size: 1463 2579
Fragments arranged by size:
2579 2573 1463 837 321 303 154 101
49 45
Bpu10I CC'TnA_GC
Cuts at: 3332 5091 3332
Size: 1759 6666
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4148 4332 4952 6550 7415 7713 4148
Size: 184 620 1598 865 298 4860
Fragments arranged by size:
4860 1598 865 620 298 184
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4050 4651 7088 882
Size: 617 2551 601 2437 2219
Fragments arranged by size:
2551 2437 2219 617 601
BsaAI yAC'GTr
Cuts at: 493 1204 5722 6216 493
Size: 711 4518 494 2702
Fragments arranged by size:
4518 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 8419 8419
Size: 8425
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6232 6614 7249 275
Size: 53 83 186 936 4699 382 635 1451
Fragments arranged by size:
4699 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4407 6858 7256 7530 7677 8088 4407
Size: 2451 398 274 147 411 4744
Fragments arranged by size:
4744 2451 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4629 4659 4992 5022 1178
Size: 30 165 30 3226 30 333 30 4581
Fragments arranged by size:
4581 3226 333 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3227 4151 4639 5000 1186
Size: 2041 924 488 361 4611
Fragments arranged by size:
4611 2041 924 488 361
BseYI C'CCAG_C
Cuts at: 4581 5569 7628 8124 4581
Size: 988 2059 496 4882
Fragments arranged by size:
4882 2059 988 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5338 1163
Size: 573 3602 4250
Fragments arranged by size:
4250 3602 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5875 6636 7664 1540
Size: 275 186 3874 761 1028 2301
Fragments arranged by size:
3874 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2851 3333 4122 4519 4907 6486 6571 7642 2851
Size: 482 789 397 388 1579 85 1071 3634
Fragments arranged by size:
3634 1579 1071 789 482 397 388 85
BsmI GAATG_Cn'
Cuts at: 5170 5936 6029 5170
Size: 766 93 7566
Fragments arranged by size:
7566 766 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3524 3830 4166 3524
Size: 306 336 7783
Fragments arranged by size:
7783 336 306
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2851 2883 3333 4122 4519 4621
Size: 399 340 721 32 450 789 397 102
Cuts at: 4621 4907 5408 5662 5744 6486 6571 7642 1391
Size: 286 501 254 82 742 85 1071 2174
Fragments arranged by size:
2174 1071 789 742 721 501 450 399
397 340 286 254 102 85 82 32
BspEI T'CCGG_A
Cuts at: 4407 8088 4407
Size: 3681 4744
BspHI T'CATG_A
Cuts at: 6314 8044 6314
Size: 1730 6695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3435 3528 5536 5780 844
Size: 2591 93 2008 244 3489
Fragments arranged by size:
3489 2591 2008 244 93
BsrBI CCG'CTC
Cuts at: 1394 1823 6312 1394
Size: 429 4489 3507
Fragments arranged by size:
4489 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6920 7094 65
Size: 1183 5672 174 1396
Fragments arranged by size:
5672 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7069 7256 1700
Size: 110 5259 187 2869
Fragments arranged by size:
5259 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8425
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8425
BssSI C'ACGA_G
Cuts at: 2487 5106 6485 7497 2487
Size: 2619 1379 1012 3415
Fragments arranged by size:
3415 2619 1379 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2330 4148 4543 4723 794
Size: 1536 1818 395 180 4496
Fragments arranged by size:
4496 1818 1536 395 180
BstBI TT'CG_AA
Cuts at: 2007 7229 2007
Size: 5222 3203
BstEII G'GTnAC_C
Cuts at: 1184 5464 1184
Size: 4280 4145
BstXI CCAn_nnnn'nTGG
Cuts at: 4603 4603
Size: 8425
BstYI r'GATC_y
Cuts at: 1972 2470 2518 3146 3358 4338 5149 5452 5517
Size: 498 48 628 212 980 811 303 65
Cuts at: 5517 5755 6122 6507 6524 7976 8062 8074 8420
Size: 238 367 385 17 1452 86 12 346
Cuts at: 8420 1972
Size: 1977
Fragments arranged by size:
1977 1452 980 811 628 498 385 367
346 303 238 212 86 65 48 17
12
Bsu36I CC'TnA_GG
Cuts at: 2139 2523 5043 2139
Size: 384 2520 5521
Fragments arranged by size:
5521 2520 384
BtgI C'CryG_G
Cuts at: 513 1065 2628 3127 4222 513
Size: 552 1563 499 1095 4716
Fragments arranged by size:
4716 1563 1095 552 499
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5550 487
Size: 683 56 4324 3362
Fragments arranged by size:
4324 3362 683 56
BtsI GCAGTG_nn'
Cuts at: 4236 5315 5941 6735 6755 4236
Size: 1079 626 794 20 5906
Fragments arranged by size:
5906 1079 794 626 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2851 2886 5293 5328 529
Size: 35 2287 35 2407 35 3626
Fragments arranged by size:
3626 2407 2287 35 35 35
DraI TTT'AAA
Cuts at: 2254 5729 2254
Size: 3475 4950
DraIII CAC_nnn'GTG
Cuts at: 5370 5370
Size: 8425
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3538 7432 809
Size: 507 2222 3894 1802
Fragments arranged by size:
3894 2222 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5664 5872 6761
Size: 54 1425 50 275 18 3834 208 889
Cuts at: 6761 8390 8
Size: 1629 43
Fragments arranged by size:
3834 1629 1425 889 275 208 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5872 1537
Size: 275 4060 4090
Fragments arranged by size:
4090 4060 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2025 2393 3321 5074 5703 6355 1353
Size: 672 368 928 1753 629 652 3423
Fragments arranged by size:
3423 1753 928 672 652 629 368
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7012 7386 7532 387
Size: 1064 138 5423 374 146 1280
Fragments arranged by size:
5423 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3331 3331
Size: 8425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2799 3752 3812 3857 3878 4160 4452
Size: 393 1167 953 60 45 21 282 292
Cuts at: 4452 4931 4997 5046 5147 5468 5622 6488 7085
Size: 479 66 49 101 321 154 866 597
Cuts at: 7085 7872 8140 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1167 953 866 787 597 479 393
321 292 282 268 154 101 66 60
49 45 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2526 3209 5012 2526
Size: 683 1803 5939
Fragments arranged by size:
5939 1803 683
EcoO109I rG'GnC_Cy
Cuts at: 3634 3634
Size: 8425
EcoRI G'AATT_C
Cuts at: 2043 4505 5734 6112 2043
Size: 2462 1229 378 4356
Fragments arranged by size:
4356 2462 1229 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2431 2890 5416 2431
Size: 459 2526 5440
Fragments arranged by size:
5440 2526 459
FspI TGC'GCA
Cuts at: 6931 6931
Size: 8425
HaeII r_GCGC'y
Cuts at: 1678 2063 2234 3620 3814 7572 1678
Size: 385 171 1386 194 3758 2531
Fragments arranged by size:
3758 2531 1386 385 194 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4330 4362 4629 4661 7066
Size: 32 135 32 2758 32 267 32 2405
Cuts at: 7066 7098 7140 7172 1373
Size: 32 42 32 2626
Fragments arranged by size:
2758 2626 2405 267 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2901 5769 6015 669
Size: 988 281 963 2868 246 3079
Fragments arranged by size:
3079 2868 988 963 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8425
HpaI GTT'AAC
Cuts at: 6015 6015
Size: 8425
KpnI G_GTAC'C
Cuts at: 3138 5750 3138
Size: 2612 5813
MfeI C'AATT_G
Cuts at: 3434 6024 3434
Size: 2590 5835
MluI A'CGCG_T
Cuts at: 8301 8301
Size: 8425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3252 5354 7539 7723 1305
Size: 1947 2102 2185 184 2007
Fragments arranged by size:
2185 2102 2007 1947 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5666 10
Size: 54 1425 343 3834 2769
Fragments arranged by size:
3834 2769 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2633 4314 4912 5190 6383 6742 6901
Size: 648 1467 1681 598 278 1193 359 159
Cuts at: 6901 518
Size: 2042
Fragments arranged by size:
2042 1681 1467 1193 648 598 359 278
159
MspA1I CmG'CkG
Cuts at: 1606 3068 3251 3961 4148 4704 4834 4897 5841
Size: 1462 183 710 187 556 130 63 944
Cuts at: 5841 6518 7666 7911 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1462 1148 944 710 677 556 245
187 183 130 63
NcoI C'CATG_G
Cuts at: 513 1065 3127 513
Size: 552 2062 5811
Fragments arranged by size:
5811 2062 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8425
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8425
NotI GC'GGCC_GC
Cuts at: 5872 5872
Size: 8425
NspI r_CATG'y
Cuts at: 1132 1229 3344 3569 4717 5609 5783 7328 1132
Size: 97 2115 225 1148 892 174 1545 2229
Fragments arranged by size:
2229 2115 1545 1148 892 225 174 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8425
PciI A'CATG_T
Cuts at: 5605 7324 5605
Size: 1719 6706
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2357 3271 3835 1108
Size: 1249 914 564 5698
Fragments arranged by size:
5698 1249 914 564
PfoI T'CCnGG_A
Cuts at: 3828 5124 5258 3828
Size: 1296 134 6995
Fragments arranged by size:
6995 1296 134
PmeI GTTT'AAAC
Cuts at: 5729 5729
Size: 8425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6494 6526 8007 8039 6494
Size: 32 1481 32 6880
Fragments arranged by size:
6880 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8425
PsiI TTA'TAA
Cuts at: 5995 5995
Size: 8425
PspXI vC'TCGA_Gb
Cuts at: 4245 4245
Size: 8425
PstI C_TGCA'G
Cuts at: 830 2335 3432 3481 3675 4243 4839 5777 830
Size: 1505 1097 49 194 568 596 938 3478
Fragments arranged by size:
3478 1505 1097 938 596 568 194 49
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8425
PvuII CAG'CTG
Cuts at: 3068 3251 4148 4704 4834 4897 3068
Size: 183 897 556 130 63 6596
Fragments arranged by size:
6596 897 556 183 130 63
SacI G_AGCT'C
Cuts at: 3333 3333
Size: 8425
SalI G'TCGA_C
Cuts at: 1936 5767 1936
Size: 3831 4594
SapI GCTCTTCn'nnn_
Cuts at: 2393 3321 2393
Size: 928 7497
SbfI CC_TGCA'GG
Cuts at: 5777 5777
Size: 8425
ScaI AGT'ACT
Cuts at: 4291 6673 8385 4291
Size: 2382 1712 4331
Fragments arranged by size:
4331 2382 1712
SfcI C'TryA_G
Cuts at: 826 1045 2331 2884 3119 3428 3477 3671 4239
Size: 219 1286 553 235 309 49 194 568
Cuts at: 4239 4532 4835 5773 6908 7589 7780 826
Size: 293 303 938 1135 681 191 1471
Fragments arranged by size:
1471 1286 1135 938 681 568 553 309
303 293 235 219 194 191 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8425
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8425
SmaI CCC'GGG
Cuts at: 1872 5752 1872
Size: 3880 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4163 4245 4347 4931 6529 7430
Size: 197 926 2220 82 102 584 1598 901
Cuts at: 7430 7692 820
Size: 262 1553
Fragments arranged by size:
2220 1598 1553 926 901 584 262 197
102 82
SnaBI TAC'GTA
Cuts at: 493 5722 6216 493
Size: 5229 494 2702
Fragments arranged by size:
5229 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8425
SphI G_CATG'C
Cuts at: 1132 3344 3569 5783 1132
Size: 2212 225 2214 3774
Fragments arranged by size:
3774 2214 2212 225
SspI AAT'ATT
Cuts at: 5 52 2809 3052 4591 4628 5159 6349 5
Size: 47 2757 243 1539 37 531 1190 2081
Fragments arranged by size:
2757 2081 1539 1190 531 243 47 37
StuI AGG'CCT
Cuts at: 1517 2820 1517
Size: 1303 7122
StyI C'CwwG_G
Cuts at: 513 1065 2369 2574 2772 3127 5272 5864 513
Size: 552 1304 205 198 355 2145 592 3074
Fragments arranged by size:
3074 2145 1304 592 552 355 205 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5304 6452 6469 6622 6807 7252 325
Size: 1255 3724 1148 17 153 185 445 1498
Fragments arranged by size:
3724 1498 1255 1148 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4289 6671 8383
Size: 274 80 33 51 1443 2312 2382 1712
Cuts at: 8383 96
Size: 138
Fragments arranged by size:
2382 2312 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4598 4630 8385 8417 4598
Size: 32 3755 32 4606
Fragments arranged by size:
4606 3755 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8425
XbaI T'CTAG_A
Cuts at: 2980 5761 2980
Size: 2781 5644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4578 4578
Size: 8425
XhoI C'TCGA_G
Cuts at: 1943 4245 1943
Size: 2302 6123
XmaI C'CCGG_G
Cuts at: 1870 5750 1870
Size: 3880 4545
XmnI GAAnn'nnTTC
Cuts at: 3756 6554 3756
Size: 2798 5627
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6233 276
Size: 53 83 186 936 4699 2468
Fragments arranged by size:
4699 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BbeI BbvCI BfrBI BsiWI BstZ17I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI