Restriction map for
pFN21ASDB3041
(Linear) (Six-base) MAPSORT of: KIBB3041.seq Check: 1872 from: 1 to: 3696
KIBB3041.orf
With 182 enzymes: SgfI *
January 7, 2013 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3513 3696
Size: 3513 183
AccI GT'mk_AC
Cuts at: 0 716 3696
Size: 716 2980
Acc65I G'GTAC_C
Cuts at: 0 1111 3696
Size: 1111 2585
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1729 1855 2429 2908 3696
Size: 776 953 126 574 479 788
Fragments arranged by size:
953 788 776 574 479 126
AfeI AGC'GCT
Cuts at: 0 1595 3696
Size: 1595 2101
AflIII A'CryG_T
Cuts at: 0 3582 3696
Size: 3582 114
AleI CACnn'nnGTG
Cuts at: 0 2889 3696
Size: 2889 807
AlwNI CAG_nnn'CTG
Cuts at: 0 1182 1812 2125 2520 2874 2877 2979 3528
Size: 1182 630 313 395 354 3 102 549
Cuts at: 3528 3696
Size: 168
Fragments arranged by size:
1182 630 549 395 354 313 168 102
3
ApoI r'AATT_y
Cuts at: 0 20 397 440 2482 3696
Size: 20 377 43 2042 1214
Fragments arranged by size:
2042 1214 377 43 20
AseI AT'TA_AT
Cuts at: 0 782 3696
Size: 782 2914
AvaI C'yCGr_G
Cuts at: 0 2222 3696
Size: 2222 1474
AvrII C'CTAG_G
Cuts at: 0 346 3696
Size: 346 3350
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1457 1490 3696
Size: 1457 33 2206
Fragments arranged by size:
2206 1457 33
BamHI G'GATC_C
Cuts at: 0 495 3429 3696
Size: 495 2934 267
Fragments arranged by size:
2934 495 267
BanI G'GyrC_C
Cuts at: 0 1111 3696
Size: 1111 2585
BanII G_rGCy'C
Cuts at: 0 1310 3639 3696
Size: 1310 2329 57
Fragments arranged by size:
2329 1310 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 183 541 1370 1511 2546 3544 3696
Size: 183 358 829 141 1035 998 152
Fragments arranged by size:
1035 998 829 358 183 152 141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3102 3136 3696
Size: 3102 34 560
Fragments arranged by size:
3102 560 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 549 3018 3696
Size: 549 2469 678
Fragments arranged by size:
2469 678 549
BglI GCCn_nnn'nGGC
Cuts at: 0 607 3365 3696
Size: 607 2758 331
Fragments arranged by size:
2758 607 331
BlpI GC'TnA_GC
Cuts at: 0 2753 2878 3696
Size: 2753 125 818
Fragments arranged by size:
2753 818 125
Bme1580I G_kGCm'C
Cuts at: 0 107 860 2598 3385 3696
Size: 107 753 1738 787 311
Fragments arranged by size:
1738 787 753 311 107
BmrI ACTGGGnnnn_n'
Cuts at: 0 615 1416 3696
Size: 615 801 2280
Fragments arranged by size:
2280 801 615
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2640 3696
Size: 2640 1056
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1789 1834 2137 2974 3023 3124 3445 3599
Size: 1789 45 303 837 49 101 321 154
Cuts at: 3599 3696
Size: 97
Fragments arranged by size:
1789 837 321 303 154 101 97 49
45
Bpu10I CC'TnA_GC
Cuts at: 0 1309 3068 3696
Size: 1309 1759 628
Fragments arranged by size:
1759 1309 628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2125 2309 2929 3696
Size: 2125 184 620 767
Fragments arranged by size:
2125 767 620 184
BsaI GGTCTCn'nnnn_
Cuts at: 0 2027 2628 3696
Size: 2027 601 1068
Fragments arranged by size:
2027 1068 601
BsaWI w'CCGG_w
Cuts at: 0 2384 3696
Size: 2384 1312
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2606 2636 2969 2999 3696
Size: 2606 30 333 30 697
Fragments arranged by size:
2606 697 333 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1204 2128 2616 2977 3696
Size: 1204 924 488 361 719
Fragments arranged by size:
1204 924 719 488 361
BseYI C'CCAG_C
Cuts at: 0 2558 3546 3696
Size: 2558 988 150
Fragments arranged by size:
2558 988 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3315 3696
Size: 3315 381
BsiHKAI G_wGCw'C
Cuts at: 0 828 1310 2099 2496 2884 3696
Size: 828 482 789 397 388 812
Fragments arranged by size:
828 812 789 482 397 388
BsmI GAATG_Cn'
Cuts at: 0 3147 3696
Size: 3147 549
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1501 1807 2143 3696
Size: 1501 306 336 1553
Fragments arranged by size:
1553 1501 336 306
Bsp1286I G_dGCh'C
Cuts at: 0 107 828 860 1310 2099 2496 2598 2884
Size: 107 721 32 450 789 397 102 286
Cuts at: 2884 3385 3639 3696
Size: 501 254 57
Fragments arranged by size:
789 721 501 450 397 286 254 107
102 57 32
BspEI T'CCGG_A
Cuts at: 0 2384 3696
Size: 2384 1312
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1412 1505 3513 3696
Size: 1412 93 2008 183
Fragments arranged by size:
2008 1412 183 93
BssSI C'ACGA_G
Cuts at: 0 464 3083 3696
Size: 464 2619 613
Fragments arranged by size:
2619 613 464
BstAPI GCAn_nnn'nTGC
Cuts at: 0 307 2125 2520 2700 3696
Size: 307 1818 395 180 996
Fragments arranged by size:
1818 996 395 307 180
BstEII G'GTnAC_C
Cuts at: 0 3441 3696
Size: 3441 255
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2580 3696
Size: 2580 1116
BstYI r'GATC_y
Cuts at: 0 447 495 1123 1335 2315 3126 3429 3494
Size: 447 48 628 212 980 811 303 65
Cuts at: 3494 3696
Size: 202
Fragments arranged by size:
980 811 628 447 303 212 202 65
48
Bsu36I CC'TnA_GG
Cuts at: 0 116 500 3020 3696
Size: 116 384 2520 676
Fragments arranged by size:
2520 676 384 116
BtgI C'CryG_G
Cuts at: 0 605 1104 2199 3696
Size: 605 499 1095 1497
Fragments arranged by size:
1497 1095 605 499
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3527 3696
Size: 3527 169
BtsI GCAGTG_nn'
Cuts at: 0 2213 3292 3696
Size: 2213 1079 404
Fragments arranged by size:
2213 1079 404
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 828 863 3270 3305 3696
Size: 828 35 2407 35 391
Fragments arranged by size:
2407 828 391 35 35
DraI TTT'AAA
Cuts at: 0 231 3696
Size: 231 3465
DraIII CAC_nnn'GTG
Cuts at: 0 3347 3696
Size: 3347 349
DrdI GACnn_nn'nnGTC
Cuts at: 0 1515 3696
Size: 1515 2181
EaeI y'GGCC_r
Cuts at: 0 3641 3696
Size: 3641 55
EarI CTCTTCn'nnn_
Cuts at: 0 2 370 1298 3051 3680 3696
Size: 2 368 928 1753 629 16
Fragments arranged by size:
1753 928 629 368 16 2
EcoICRI GAG'CTC
Cuts at: 0 1308 3696
Size: 1308 2388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1729 1789 1834 1855 2137 2429 2908
Size: 776 953 60 45 21 282 292 479
Cuts at: 2908 2974 3023 3124 3445 3599 3696
Size: 66 49 101 321 154 97
Fragments arranged by size:
953 776 479 321 292 282 154 101
97 66 60 49 45 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 503 1186 2989 3696
Size: 503 683 1803 707
Fragments arranged by size:
1803 707 683 503
EcoO109I rG'GnC_Cy
Cuts at: 0 1611 3696
Size: 1611 2085
EcoRI G'AATT_C
Cuts at: 0 20 2482 3696
Size: 20 2462 1214
Fragments arranged by size:
2462 1214 20
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 408 867 3393 3696
Size: 408 459 2526 303
Fragments arranged by size:
2526 459 408 303
HaeII r_GCGC'y
Cuts at: 0 40 211 1597 1791 3696
Size: 40 171 1386 194 1905
Fragments arranged by size:
1905 1386 194 171 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2307 2339 2606 2638 3696
Size: 2307 32 267 32 1058
Fragments arranged by size:
2307 1058 267 32 32
HincII GTy'rAC
Cuts at: 0 878 3696
Size: 878 2818
KpnI G_GTAC'C
Cuts at: 0 1115 3696
Size: 1115 2581
MfeI C'AATT_G
Cuts at: 0 1411 3696
Size: 1411 2285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1229 3331 3696
Size: 1229 2102 365
Fragments arranged by size:
2102 1229 365
MscI TGG'CCA
Cuts at: 0 3643 3696
Size: 3643 53
MslI CAynn'nnrTG
Cuts at: 0 610 2291 2889 3167 3696
Size: 610 1681 598 278 529
Fragments arranged by size:
1681 610 598 529 278
MspA1I CmG'CkG
Cuts at: 0 1045 1228 1938 2125 2681 2811 2874 3696
Size: 1045 183 710 187 556 130 63 822
Fragments arranged by size:
1045 822 710 556 187 183 130 63
NcoI C'CATG_G
Cuts at: 0 1104 3696
Size: 1104 2592
NspI r_CATG'y
Cuts at: 0 1321 1546 2694 3586 3696
Size: 1321 225 1148 892 110
Fragments arranged by size:
1321 1148 892 225 110
PciI A'CATG_T
Cuts at: 0 3582 3696
Size: 3582 114
PflMI CCAn_nnn'nTGG
Cuts at: 0 334 1248 1812 3696
Size: 334 914 564 1884
Fragments arranged by size:
1884 914 564 334
PfoI T'CCnGG_A
Cuts at: 0 1805 3101 3235 3696
Size: 1805 1296 134 461
Fragments arranged by size:
1805 1296 461 134
PspXI vC'TCGA_Gb
Cuts at: 0 2222 3696
Size: 2222 1474
PstI C_TGCA'G
Cuts at: 0 312 1409 1458 1652 2220 2816 3696
Size: 312 1097 49 194 568 596 880
Fragments arranged by size:
1097 880 596 568 312 194 49
PvuII CAG'CTG
Cuts at: 0 1045 1228 2125 2681 2811 2874 3696
Size: 1045 183 897 556 130 63 822
Fragments arranged by size:
1045 897 822 556 183 130 63
SacI G_AGCT'C
Cuts at: 0 1310 3696
Size: 1310 2386
SapI GCTCTTCn'nnn_
Cuts at: 0 370 1298 3696
Size: 370 928 2398
Fragments arranged by size:
2398 928 370
ScaI AGT'ACT
Cuts at: 0 2268 3696
Size: 2268 1428
SfcI C'TryA_G
Cuts at: 0 308 861 1096 1405 1454 1648 2216 2509
Size: 308 553 235 309 49 194 568 293
Cuts at: 2509 2812 3696
Size: 303 884
Fragments arranged by size:
884 568 553 309 308 303 293 235
194 49
SmlI C'TyrA_G
Cuts at: 0 2140 2222 2324 2908 3696
Size: 2140 82 102 584 788
Fragments arranged by size:
2140 788 584 102 82
SphI G_CATG'C
Cuts at: 0 1321 1546 3696
Size: 1321 225 2150
Fragments arranged by size:
2150 1321 225
SspI AAT'ATT
Cuts at: 0 786 1029 2568 2605 3136 3696
Size: 786 243 1539 37 531 560
Fragments arranged by size:
1539 786 560 531 243 37
StuI AGG'CCT
Cuts at: 0 797 3696
Size: 797 2899
StyI C'CwwG_G
Cuts at: 0 346 551 749 1104 3249 3696
Size: 346 205 198 355 2145 447
Fragments arranged by size:
2145 447 355 346 205 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3281 3696
Size: 3281 415
TatI w'GTAC_w
Cuts at: 0 2266 3696
Size: 2266 1430
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2575 2607 3696
Size: 2575 32 1089
Fragments arranged by size:
2575 1089 32
XbaI T'CTAG_A
Cuts at: 0 957 3696
Size: 957 2739
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2555 3696
Size: 2555 1141
XhoI C'TCGA_G
Cuts at: 0 2222 3696
Size: 2222 1474
XmnI GAAnn'nnTTC
Cuts at: 0 1733 3696
Size: 1733 1963
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AlwNI
ApoI AseI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BglI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KpnI
MfeI MmeI MscI MslI MspA1I NcoI NspI PciI
PflMI PfoI PspXI PstI PvuII SacI SapI ScaI
SfcI SmlI SphI SspI StuI StyI TaqII TatI
TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AlfI AloI ApaI
ApaLI AscI AsiSI BbeI BbvCI BclI BfrBI BglII
BmgBI BmtI BsaAI BsaBI BsaHI BsiEI BsiWI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstZ17I ClaI
EagI EciI EcoRV FseI FspI FspAI HindIII HpaI
KasI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PvuI RsrII SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SwaI Tth111I XmaI ZraI