(Circular) (Six-base) MAPSORT of: pFN21ASDB1439.seq Check: 4867 from: 1 to: 11548 pFN21ASDB1439 11548 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3244 6477 3244 Size: 3233 8315 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6321 9358 278 Size: 53 83 186 936 4785 3037 2468 Fragments arranged by size: 4785 3037 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2556 8208 8827 8891 1937 Size: 619 5652 619 64 4594 Fragments arranged by size: 5652 4594 619 619 64 Acc65I G'GTAC_C Cuts at: 4587 8869 4587 Size: 4282 7266 AclI AA'CG_TT Cuts at: 1563 9675 10048 1563 Size: 8112 373 3063 Fragments arranged by size: 8112 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2642 2653 3275 3468 4088 4412 5357 5483 Size: 1010 11 622 193 620 324 945 126 Cuts at: 5483 5864 6256 6377 6876 7035 7809 8067 9611 Size: 381 392 121 499 159 774 258 1544 Cuts at: 9611 10995 11263 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1544 1384 1010 945 774 622 620 499 392 381 324 268 258 193 159 126 121 11 AfeI AGC'GCT Cuts at: 2657 2657 Size: 11548 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 11351 AflIII A'CryG_T Cuts at: 1203 3289 3657 3687 5582 10447 11424 1203 Size: 2086 368 30 1895 4865 977 1327 Fragments arranged by size: 4865 2086 1895 1327 977 368 30 AgeI A'CCGG_T Cuts at: 10379 10379 Size: 11548 AhdI GACnn_n'nnGTC Cuts at: 8784 10277 8784 Size: 1493 10055 AleI CACnn'nnGTG Cuts at: 1166 7052 7991 1166 Size: 5886 939 4723 Fragments arranged by size: 5886 4723 939 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 11548 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3644 3676 4612 4644 5697 5729 3644 Size: 32 936 32 1053 32 9463 Fragments arranged by size: 9463 1053 936 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 3151 6094 9291 10863 3151 Size: 2943 3197 1572 3836 Fragments arranged by size: 3836 3197 2943 1572 ApaI G_GGCC'C Cuts at: 3330 3479 6800 3330 Size: 149 3321 8078 Fragments arranged by size: 8078 3321 149 ApaLI G'TGCA_C Cuts at: 9605 10761 9605 Size: 1156 10392 ApoI r'AATT_y Cuts at: 1493 2871 4520 5811 6582 6668 6699 8501 8761 Size: 1378 1649 1291 771 86 31 1802 260 Cuts at: 8761 8857 9083 9235 11344 1493 Size: 96 226 152 2109 1697 Fragments arranged by size: 2109 1802 1697 1649 1378 1291 771 260 226 152 96 86 31 AseI AT'TA_AT Cuts at: 160 1032 10102 160 Size: 872 9070 1606 Fragments arranged by size: 9070 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11548 AvaI C'yCGr_G Cuts at: 1870 1943 3002 3018 3480 5308 6530 6968 8808 Size: 73 1059 16 462 1828 1222 438 1840 Cuts at: 8808 8873 1870 Size: 65 4545 Fragments arranged by size: 4545 1840 1828 1222 1059 462 438 73 65 16 BamHI G'GATC_C Cuts at: 3091 4738 8878 9245 3091 Size: 1647 4140 367 5394 Fragments arranged by size: 5394 4140 1647 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2726 4587 5447 5992 6536 Size: 325 217 627 939 1861 860 545 544 Cuts at: 6536 7868 7946 8459 8477 8869 10324 618 Size: 1332 78 513 18 392 1455 1842 Fragments arranged by size: 1861 1842 1455 1332 939 860 627 545 544 513 392 325 217 78 18 BanII G_rGCy'C Cuts at: 1391 3330 3479 4702 4719 4842 6800 6968 7181 Size: 1939 149 1223 17 123 1958 168 213 Cuts at: 7181 7749 7761 8313 8867 1391 Size: 568 12 552 554 4072 Fragments arranged by size: 4072 1958 1939 1223 568 554 552 213 168 149 123 17 12 BbeI G_GCGC'C Cuts at: 5996 8463 5996 Size: 2467 9081 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2751 3051 6413 6864 7519 928 Size: 971 852 300 3362 451 655 4957 Fragments arranged by size: 4957 3362 971 852 655 451 300 BbvCI CC'TCA_GC Cuts at: 2209 7508 2209 Size: 5299 6249 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2072 2106 5910 5944 7243 7277 8153 Size: 34 135 34 3804 34 1299 34 876 Cuts at: 8153 8187 9739 9773 1903 Size: 34 1552 34 3678 Fragments arranged by size: 3804 3678 1552 1299 876 135 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3243 3702 6645 9440 10650 3243 Size: 459 2943 2795 1210 4141 Fragments arranged by size: 4141 2943 2795 1210 459 BclI T'GATC_A Cuts at: 1548 2565 3372 3966 4594 1548 Size: 1017 807 594 628 8502 Fragments arranged by size: 8502 1017 807 628 594 BfrBI ATG'CAT Cuts at: 3137 4183 3137 Size: 1046 10502 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4301 7313 7400 Size: 107 122 71 631 605 2629 3012 87 Cuts at: 7400 8226 10159 136 Size: 826 1933 1525 Fragments arranged by size: 3012 2629 1933 1525 826 631 605 122 107 87 71 BglII A'GATC_T Cuts at: 2834 3333 11543 2834 Size: 499 8210 2839 Fragments arranged by size: 8210 2839 499 BlpI GC'TnA_GC Cuts at: 4274 4386 5085 5892 8965 4274 Size: 112 699 807 3073 6857 Fragments arranged by size: 6857 3073 807 699 112 Bme1580I G_kGCm'C Cuts at: 1790 2729 3330 3479 3637 6041 6530 6800 7871 Size: 939 601 149 158 2404 489 270 1071 Cuts at: 7871 8480 9609 10765 1790 Size: 609 1129 1156 2573 Fragments arranged by size: 2573 2404 1156 1129 1071 939 609 601 489 270 158 149 BmgBI CAC'GTC Cuts at: 1318 3751 5596 1318 Size: 2433 1845 7270 Fragments arranged by size: 7270 2433 1845 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6716 7201 7645 Size: 416 53 83 399 24 5299 485 444 Cuts at: 7645 7989 9279 10237 442 Size: 344 1290 958 1753 Fragments arranged by size: 5299 1753 1290 958 485 444 416 399 344 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 11548 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6734 7042 7633 8740 6734 Size: 308 591 1107 9542 Fragments arranged by size: 9542 1107 591 308 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 2672 3173 3538 3593 3668 3891 3908 Size: 977 456 501 365 55 75 223 17 Cuts at: 3908 5162 5595 5792 6062 7142 7275 7858 7935 Size: 1254 433 197 270 1080 133 583 77 Cuts at: 7935 8200 8624 8838 10208 1239 Size: 265 424 214 1370 2579 Fragments arranged by size: 2579 1370 1254 1080 977 583 501 456 433 424 365 270 265 223 214 197 133 77 75 55 17 Bpu10I CC'TnA_GC Cuts at: 2209 5199 5347 5443 7490 7508 2209 Size: 2990 148 96 2047 18 6249 Fragments arranged by size: 6249 2990 2047 148 96 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2559 2802 7855 8477 9673 10538 10836 2559 Size: 243 5053 622 1196 865 298 3271 Fragments arranged by size: 5053 3271 1196 865 622 298 243 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2945 4067 5801 8656 10211 882 Size: 617 1446 1122 1734 2855 1555 2219 Fragments arranged by size: 2855 2219 1734 1555 1446 1122 617 BsaAI yAC'GTr Cuts at: 493 1204 3721 5583 8232 8845 9339 493 Size: 711 2517 1862 2649 613 494 2702 Fragments arranged by size: 2702 2649 2517 1862 711 613 494 BsaBI GATnn'nnATC Cuts at: 2972 4154 4175 11542 2972 Size: 1182 21 7367 2978 Fragments arranged by size: 7367 2978 1182 21 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5993 6318 8460 9355 Size: 53 83 186 936 4460 325 2142 895 Cuts at: 9355 9737 10372 275 Size: 382 635 1451 Fragments arranged by size: 4460 2142 1451 936 895 635 382 325 186 83 53 BsaWI w'CCGG_w Cuts at: 2439 3825 4417 6373 7479 9981 10379 10653 10800 Size: 1386 592 1956 1106 2502 398 274 147 Cuts at: 10800 11211 2439 Size: 411 2776 Fragments arranged by size: 2776 2502 1956 1386 1106 592 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4007 4037 4043 4073 4121 Size: 30 165 30 2604 30 6 30 48 Cuts at: 4121 4151 1178 Size: 30 8575 Fragments arranged by size: 8575 2604 165 48 30 30 30 30 30 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 5144 5789 6986 7625 8644 8647 8650 Size: 1162 2796 645 1197 639 1019 3 3 Cuts at: 8650 8675 8678 8732 1186 Size: 25 3 54 4002 Fragments arranged by size: 4002 2796 1197 1162 1019 645 639 54 25 3 3 3 BseYI C'CCAG_C Cuts at: 4922 5106 5136 6435 7102 7238 7360 7385 7879 Size: 184 30 1299 667 136 122 25 494 Cuts at: 7879 10751 11247 4922 Size: 2872 496 5223 Fragments arranged by size: 5223 2872 1299 667 496 494 184 136 122 30 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3914 6107 6511 8633 8786 1163 Size: 573 2178 2193 404 2122 153 3925 Fragments arranged by size: 3925 2193 2178 2122 573 404 153 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2894 8998 9759 10787 1540 Size: 275 186 893 6104 761 1028 2301 Fragments arranged by size: 6104 2301 1028 893 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2714 3027 4749 4986 5091 6159 7617 9609 9694 Size: 313 1722 237 105 1068 1458 1992 85 Cuts at: 9694 10765 2714 Size: 1071 3497 Fragments arranged by size: 3497 1992 1722 1458 1071 1068 313 237 105 85 BsmI GAATG_Cn' Cuts at: 2158 2186 2223 2371 3265 4901 9059 9152 2158 Size: 28 37 148 894 1636 4158 93 4554 Fragments arranged by size: 4554 4158 1636 894 148 93 37 28 BsmBI CGTCTCn'nnnn_ Cuts at: 2671 5305 5771 2671 Size: 2634 466 8448 Fragments arranged by size: 8448 2634 466 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2714 2729 3027 3330 3479 3637 4702 Size: 399 924 15 298 303 149 158 1065 Cuts at: 4702 4719 4749 4842 4986 5091 6041 6159 6530 Size: 17 30 93 144 105 950 118 371 Cuts at: 6530 6800 6968 7181 7617 7749 7761 7871 8313 Size: 270 168 213 436 132 12 110 442 Cuts at: 8313 8480 8867 9609 9694 10765 1391 Size: 167 387 742 85 1071 2174 Fragments arranged by size: 2174 1071 1065 950 924 742 442 436 399 387 371 303 298 270 213 168 167 158 149 144 132 118 110 105 93 85 30 17 15 12 BspEI T'CCGG_A Cuts at: 2439 6373 11211 2439 Size: 3934 4838 2776 Fragments arranged by size: 4838 3934 2776 BspHI T'CATG_A Cuts at: 2176 2361 3963 6780 7218 8788 9437 11167 2176 Size: 185 1602 2817 438 1570 649 1730 2557 Fragments arranged by size: 2817 2557 1730 1602 1570 649 438 185 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3244 4350 5558 6477 8562 8903 844 Size: 2400 1106 1208 919 2085 341 3489 Fragments arranged by size: 3489 2400 2085 1208 1106 919 341 BsrBI CCG'CTC Cuts at: 1394 1823 2164 6792 7135 9435 1394 Size: 429 341 4628 343 2300 3507 Fragments arranged by size: 4628 3507 2300 429 343 341 BsrDI GCAATG_nn' Cuts at: 65 1248 2254 5241 5409 5418 10043 10217 65 Size: 1183 1006 2987 168 9 4625 174 1396 Fragments arranged by size: 4625 2987 1396 1183 1006 174 168 9 BsrFI r'CCGG_y Cuts at: 1700 1810 5995 7949 10192 10379 1700 Size: 110 4185 1954 2243 187 2869 Fragments arranged by size: 4185 2869 2243 1954 187 110 BsrGI T'GTAC_A Cuts at: 96 3219 96 Size: 3123 8425 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11548 BssSI C'ACGA_G Cuts at: 2108 9608 10620 2108 Size: 7500 1012 3036 Fragments arranged by size: 7500 3036 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2908 4549 6094 6154 6351 794 Size: 2114 1641 1545 60 197 5991 Fragments arranged by size: 5991 2114 1641 1545 197 60 BstBI TT'CG_AA Cuts at: 2007 10352 2007 Size: 8345 3203 BstEII G'GTnAC_C Cuts at: 1184 2917 7195 1184 Size: 1733 4278 5537 Fragments arranged by size: 5537 4278 1733 BstXI CCAn_nnnn'nTGG Cuts at: 2387 4632 7054 7672 8323 2387 Size: 2245 2422 618 651 5612 Fragments arranged by size: 5612 2422 2245 651 618 BstYI r'GATC_y Cuts at: 1972 2238 2834 3091 3333 3732 4011 4738 5326 Size: 266 596 257 242 399 279 727 588 Cuts at: 5326 8673 8878 9245 9630 9647 11099 11185 11197 Size: 3347 205 367 385 17 1452 86 12 Cuts at: 11197 11543 1972 Size: 346 1977 Fragments arranged by size: 3347 1977 1452 727 596 588 399 385 367 346 279 266 257 242 205 86 17 12 Bsu36I CC'TnA_GG Cuts at: 6954 7999 6954 Size: 1045 10503 BtgI C'CryG_G Cuts at: 513 1065 2022 2225 3813 4506 7665 513 Size: 552 957 203 1588 693 3159 4396 Fragments arranged by size: 4396 3159 1588 957 693 552 203 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2216 2614 8254 487 Size: 683 56 990 398 5640 3781 Fragments arranged by size: 5640 3781 990 683 398 56 BtsI GCAGTG_nn' Cuts at: 4110 4245 4487 5545 6298 7316 7447 7890 8614 Size: 135 242 1058 753 1018 131 443 724 Cuts at: 8614 9064 9858 9878 4110 Size: 450 794 20 5780 Fragments arranged by size: 5780 1058 1018 794 753 724 450 443 242 135 131 20 ClaI AT'CG_AT Cuts at: 1554 8091 1554 Size: 6537 5011 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5667 5702 529 Size: 35 5103 35 6375 Fragments arranged by size: 6375 5103 35 35 DraI TTT'AAA Cuts at: 8852 8852 Size: 11548 DraIII CAC_nnn'GTG Cuts at: 5583 5583 Size: 11548 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2552 5975 10555 809 Size: 507 1236 3423 4580 1802 Fragments arranged by size: 4580 3423 1802 1236 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2389 2891 3504 Size: 54 1425 50 275 18 559 502 613 Cuts at: 3504 4846 6772 8442 8995 9884 11513 8 Size: 1342 1926 1670 553 889 1629 43 Fragments arranged by size: 1926 1670 1629 1425 1342 889 613 559 553 502 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2891 8995 1537 Size: 275 1079 6104 4090 Fragments arranged by size: 6104 4090 1079 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2461 2786 4031 4226 4455 5187 5462 5826 Size: 1108 325 1245 195 229 732 275 364 Cuts at: 5826 9478 1353 Size: 3652 3423 Fragments arranged by size: 3652 3423 1245 1108 732 364 325 275 229 195 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3542 3914 10135 10509 10655 387 Size: 1064 138 1953 372 6221 374 146 1280 Fragments arranged by size: 6221 1953 1280 1064 374 372 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 2642 2653 2672 3173 3275 3468 Size: 393 584 426 11 19 501 102 193 Cuts at: 3468 3538 3593 3668 3891 3908 4088 4412 5162 Size: 70 55 75 223 17 180 324 750 Cuts at: 5162 5357 5483 5595 5792 5864 6062 6256 6377 Size: 195 126 112 197 72 198 194 121 Cuts at: 6377 6876 7035 7142 7275 7809 7858 7935 8067 Size: 499 159 107 133 534 49 77 132 Cuts at: 8067 8200 8624 8838 9611 10208 10995 11263 1239 Size: 133 424 214 773 597 787 268 1524 Fragments arranged by size: 1524 787 773 750 597 584 534 501 499 426 424 393 324 268 223 214 198 197 195 194 193 180 159 133 133 132 126 121 112 107 102 77 75 72 70 55 49 19 17 11 EcoNI CCTnn'n_nnAGG Cuts at: 2720 3470 4595 4724 6953 7013 2720 Size: 750 1125 129 2229 60 7255 Fragments arranged by size: 7255 2229 1125 750 129 60 EcoO109I rG'GnC_Cy Cuts at: 2644 2925 3326 3327 3475 3476 5203 5547 5870 Size: 281 401 1 148 1 1727 344 323 Cuts at: 5870 5936 6544 6796 6797 7552 7639 2644 Size: 66 608 252 1 755 87 6553 Fragments arranged by size: 6553 1727 755 608 401 344 323 281 252 148 87 66 1 1 1 EcoRI G'AATT_C Cuts at: 2871 4520 8857 9235 2871 Size: 1649 4337 378 5184 Fragments arranged by size: 5184 4337 1649 378 EcoRV GAT'ATC Cuts at: 2488 2488 Size: 11548 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2110 4093 5488 6355 6386 8479 2110 Size: 1983 1395 867 31 2093 5179 Fragments arranged by size: 5179 2093 1983 1395 867 31 FspI TGC'GCA Cuts at: 5504 10054 5504 Size: 4550 6998 HaeII r_GCGC'y Cuts at: 1678 2621 2659 5892 5996 6095 7717 8463 10695 Size: 943 38 3233 104 99 1622 746 2232 Cuts at: 10695 1678 Size: 2531 Fragments arranged by size: 3233 2531 2232 1622 943 746 104 99 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2557 2589 3691 3723 4121 Size: 32 135 32 985 32 1102 32 398 Cuts at: 4121 4153 5291 5304 5323 5336 5895 5927 7326 Size: 32 1138 13 19 13 559 32 1399 Cuts at: 7326 7358 8770 8802 10189 10221 10263 10295 1373 Size: 32 1412 32 1387 32 42 32 2626 Fragments arranged by size: 2626 1412 1399 1387 1138 1102 985 559 398 135 42 32 32 32 32 32 32 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 5914 6469 8828 8892 9138 669 Size: 988 281 3976 555 2359 64 246 3079 Fragments arranged by size: 3976 3079 2359 988 555 281 246 64 HindIII A'AGCT_T Cuts at: 748 748 Size: 11548 HpaI GTT'AAC Cuts at: 9138 9138 Size: 11548 KasI G'GCGC_C Cuts at: 5992 8459 5992 Size: 2467 9081 KpnI G_GTAC'C Cuts at: 4591 8873 4591 Size: 4282 7266 MfeI C'AATT_G Cuts at: 9147 9147 Size: 11548 MluI A'CGCG_T Cuts at: 11424 11424 Size: 11548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3892 5139 6136 6639 7882 10662 10846 1305 Size: 2587 1247 997 503 1243 2780 184 2007 Fragments arranged by size: 2780 2587 2007 1247 1243 997 503 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3506 4848 8444 10 Size: 54 1425 343 1674 1342 3596 3114 Fragments arranged by size: 3596 3114 1674 1425 1342 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3362 3749 4031 4148 4256 4544 5363 Size: 648 2196 387 282 117 108 288 819 Cuts at: 5363 5598 6785 6869 7052 7076 7349 7670 7991 Size: 235 1187 84 183 24 273 321 321 Cuts at: 7991 8321 9506 9865 10024 518 Size: 330 1185 359 159 2042 Fragments arranged by size: 2196 2042 1187 1185 819 648 387 359 330 321 321 288 282 273 235 183 159 117 108 84 24 MspA1I CmG'CkG Cuts at: 1606 3981 4385 4856 5110 5140 5556 6070 6351 Size: 2375 404 471 254 30 416 514 281 Cuts at: 6351 6439 8293 8964 9641 10789 11034 1606 Size: 88 1854 671 677 1148 245 2120 Fragments arranged by size: 2375 2120 1854 1148 677 671 514 471 416 404 281 254 245 88 30 NaeI GCC'GGC Cuts at: 5997 7951 5997 Size: 1954 9594 NarI GG'CG_CC Cuts at: 5993 8460 5993 Size: 2467 9081 NcoI C'CATG_G Cuts at: 513 1065 2022 7665 513 Size: 552 957 5643 4396 Fragments arranged by size: 5643 4396 957 552 NdeI CA'TA_TG Cuts at: 387 5749 387 Size: 5362 6186 NgoMIV G'CCGG_C Cuts at: 5995 7949 5995 Size: 1954 9594 NheI G'CTAG_C Cuts at: 1052 1052 Size: 11548 NotI GC'GGCC_GC Cuts at: 8995 8995 Size: 11548 NsiI A_TGCA'T Cuts at: 3139 4185 3139 Size: 1046 10502 NspI r_CATG'y Cuts at: 1132 1229 3661 3691 5044 5095 5221 6086 6899 Size: 97 2432 30 1353 51 126 865 813 Cuts at: 6899 8347 8638 8906 10451 1132 Size: 1448 291 268 1545 2229 Fragments arranged by size: 2432 2229 1545 1448 1353 865 813 291 268 126 97 51 30 PasI CC'CwG_GG Cuts at: 1348 2722 3385 5425 5987 1348 Size: 1374 663 2040 562 6909 Fragments arranged by size: 6909 2040 1374 663 562 PciI A'CATG_T Cuts at: 3657 3687 10447 3657 Size: 30 6760 4758 Fragments arranged by size: 6760 4758 30 PflMI CCAn_nnn'nTGG Cuts at: 1108 2428 3151 4973 6341 7191 8410 1108 Size: 1320 723 1822 1368 850 1219 4246 Fragments arranged by size: 4246 1822 1368 1320 1219 850 723 PfoI T'CCnGG_A Cuts at: 2293 2607 2649 3585 4125 4606 5622 7128 2293 Size: 314 42 936 540 481 1016 1506 6713 Fragments arranged by size: 6713 1506 1016 936 540 481 314 42 PmeI GTTT'AAAC Cuts at: 8852 8852 Size: 11548 PmlI CAC'GTG Cuts at: 3721 5583 3721 Size: 1862 9686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 9617 9649 11130 11162 9617 Size: 32 1481 32 10003 Fragments arranged by size: 10003 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5203 5547 5870 5936 7639 5203 Size: 344 323 66 1703 9112 Fragments arranged by size: 9112 1703 344 323 66 PshAI GACnn'nnGTC Cuts at: 1613 5793 6464 7589 1613 Size: 4180 671 1125 5572 Fragments arranged by size: 5572 4180 1125 671 PsiI TTA'TAA Cuts at: 6647 9118 6647 Size: 2471 9077 PspOMI G'GGCC_C Cuts at: 3326 3475 6796 3326 Size: 149 3321 8078 Fragments arranged by size: 8078 3321 149 PspXI vC'TCGA_Gb Cuts at: 8808 8808 Size: 11548 PstI C_TGCA'G Cuts at: 830 2639 3241 3858 4482 5555 6488 8900 830 Size: 1809 602 617 624 1073 933 2412 3478 Fragments arranged by size: 3478 2412 1809 1073 933 624 617 602 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11548 PvuII CAG'CTG Cuts at: 4856 5110 5140 5556 6070 6439 4856 Size: 254 30 416 514 369 9965 Fragments arranged by size: 9965 514 416 369 254 30 SalI G'TCGA_C Cuts at: 1936 8826 8890 1936 Size: 6890 64 4594 Fragments arranged by size: 6890 4594 64 SanDI GG'GwC_CC Cuts at: 5936 5936 Size: 11548 SapI GCTCTTCn'nnn_ Cuts at: 2786 4031 4455 2786 Size: 1245 424 9879 Fragments arranged by size: 9879 1245 424 SbfI CC_TGCA'GG Cuts at: 6488 8900 6488 Size: 2412 9136 ScaI AGT'ACT Cuts at: 8723 9796 11508 8723 Size: 1073 1712 8763 Fragments arranged by size: 8763 1712 1073 SexAI A'CCwGG_T Cuts at: 4118 4446 8748 4118 Size: 328 4302 6918 Fragments arranged by size: 6918 4302 328 SfcI C'TryA_G Cuts at: 826 1045 2167 2635 2732 3237 3854 4312 4478 Size: 219 1122 468 97 505 617 458 166 Cuts at: 4478 5551 6484 8896 10031 10712 10903 826 Size: 1073 933 2412 1135 681 191 1471 Fragments arranged by size: 2412 1471 1135 1122 1073 933 681 617 505 468 458 219 191 166 97 SfoI GGC'GCC Cuts at: 5994 8461 5994 Size: 2467 9081 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11548 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11548 SmaI CCC'GGG Cuts at: 1872 8875 1872 Size: 7003 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2574 2817 5308 7834 8492 8808 Size: 197 926 631 243 2491 2526 658 316 Cuts at: 8808 9652 10553 10815 820 Size: 844 901 262 1553 Fragments arranged by size: 2526 2491 1553 926 901 844 658 631 316 262 243 197 SnaBI TAC'GTA Cuts at: 493 8845 9339 493 Size: 8352 494 2702 Fragments arranged by size: 8352 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 11548 SphI G_CATG'C Cuts at: 1132 5221 6899 8347 8906 1132 Size: 4089 1678 1448 559 3774 Fragments arranged by size: 4089 3774 1678 1448 559 SspI AAT'ATT Cuts at: 5 52 2850 8041 9472 5 Size: 47 2798 5191 1431 2081 Fragments arranged by size: 5191 2798 2081 1431 47 StuI AGG'CCT Cuts at: 1517 3062 4470 5172 6815 1517 Size: 1545 1408 702 1643 6250 Fragments arranged by size: 6250 1643 1545 1408 702 StyI C'CwwG_G Cuts at: 513 1065 2022 2386 4290 4631 5035 5262 5398 Size: 552 957 364 1904 341 404 227 136 Cuts at: 5398 5496 5791 6769 7014 7665 8987 513 Size: 98 295 978 245 651 1322 3074 Fragments arranged by size: 3074 1904 1322 978 957 651 552 404 364 341 295 245 227 136 98 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2905 3641 4727 4764 6021 6449 7183 Size: 1255 1325 736 1086 37 1257 428 734 Cuts at: 7183 9575 9592 9745 9930 10375 325 Size: 2392 17 153 185 445 1498 Fragments arranged by size: 2392 1498 1325 1257 1255 1086 736 734 445 428 185 153 37 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3219 5487 6261 Size: 274 80 33 51 1443 1242 2268 774 Cuts at: 6261 7686 8721 9794 11506 96 Size: 1425 1035 1073 1712 138 Fragments arranged by size: 2268 1712 1443 1425 1242 1073 1035 774 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2096 2128 2402 2434 2897 2929 5524 5556 5569 Size: 32 274 32 463 32 2595 32 13 Cuts at: 5569 5601 6057 6089 7880 7912 11508 11540 2096 Size: 32 456 32 1791 32 3596 32 2104 Fragments arranged by size: 3596 2595 2104 1791 463 456 274 32 32 32 32 32 32 32 32 13 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11548 XbaI T'CTAG_A Cuts at: 6847 8884 6847 Size: 2037 9511 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4297 4919 5248 5293 5377 7166 7882 8320 4297 Size: 622 329 45 84 1789 716 438 7525 Fragments arranged by size: 7525 1789 716 622 438 329 84 45 XhoI C'TCGA_G Cuts at: 1943 5308 8808 1943 Size: 3365 3500 4683 Fragments arranged by size: 4683 3500 3365 XmaI C'CCGG_G Cuts at: 1870 8873 1870 Size: 7003 4545 XmnI GAAnn'nnTTC Cuts at: 7299 9677 7299 Size: 2378 9170 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6319 9356 276 Size: 53 83 186 936 4785 3037 2468 Fragments arranged by size: 4785 3037 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BsiWI BstZ17I EcoICRI FseI FspAI NruI PacI PsrI RsrII SacI SacII SfiI SrfI SwaI