(Circular) (Six-base) MAPSORT of: pFN21ASDB0025.seq Check: 6983 from: 1 to: 6421 pFN21ASDB0025 6421 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2122 2122 Size: 6421 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2106 4231 278 Size: 53 83 186 936 570 2125 2468 Fragments arranged by size: 2468 2125 936 570 186 83 53 AccI GT'mk_AC Cuts at: 1937 2106 3435 3764 1937 Size: 169 1329 329 4594 Fragments arranged by size: 4594 1329 329 169 Acc65I G'GTAC_C Cuts at: 3742 3742 Size: 6421 AclI AA'CG_TT Cuts at: 1563 4548 4921 1563 Size: 2985 373 3063 Fragments arranged by size: 3063 2985 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2721 3604 3651 4484 5868 6136 1632 Size: 1089 883 47 833 1384 268 1917 Fragments arranged by size: 1917 1384 1089 883 833 268 47 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6224 AflIII A'CryG_T Cuts at: 1203 2763 5320 6297 1203 Size: 1560 2557 977 1327 Fragments arranged by size: 2557 1560 1327 977 AgeI A'CCGG_T Cuts at: 5252 5252 Size: 6421 AhdI GACnn_n'nnGTC Cuts at: 2742 5150 2742 Size: 2408 4013 AleI CACnn'nnGTG Cuts at: 1166 2255 1166 Size: 1089 5332 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6421 AlwNI CAG_nnn'CTG Cuts at: 3431 4164 5736 3431 Size: 733 1572 4116 Fragments arranged by size: 4116 1572 733 ApaLI G'TGCA_C Cuts at: 3521 4478 5634 3521 Size: 957 1156 4308 Fragments arranged by size: 4308 1156 957 ApoI r'AATT_y Cuts at: 1493 3730 3956 4108 6217 1493 Size: 2237 226 152 2109 1697 Fragments arranged by size: 2237 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4975 160 Size: 872 3943 1606 Fragments arranged by size: 3943 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 AvaI C'yCGr_G Cuts at: 1870 1943 3746 1870 Size: 73 1803 4545 Fragments arranged by size: 4545 1803 73 BamHI G'GATC_C Cuts at: 2049 3675 3751 4118 2049 Size: 1626 76 367 4352 Fragments arranged by size: 4352 1626 367 76 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2300 2434 2911 3392 3742 Size: 325 217 627 513 134 477 481 350 Cuts at: 3742 5197 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 513 481 477 350 325 217 134 BanII G_rGCy'C Cuts at: 1391 2148 3740 1391 Size: 757 1592 4072 Fragments arranged by size: 4072 1592 757 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3045 3367 3467 928 Size: 971 1146 322 100 3882 Fragments arranged by size: 3882 1146 971 322 100 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4612 4646 1903 Size: 34 2675 34 3678 Fragments arranged by size: 3678 2675 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3195 4313 5523 3195 Size: 1118 1210 4093 Fragments arranged by size: 4093 1210 1118 BclI T'GATC_A Cuts at: 1548 2310 1548 Size: 762 5659 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3630 5032 136 Size: 107 122 71 631 605 1958 1402 1525 Fragments arranged by size: 1958 1525 1402 631 605 122 107 71 BglII A'GATC_T Cuts at: 6416 6416 Size: 6421 BlpI GC'TnA_GC Cuts at: 3168 3838 3168 Size: 670 5751 Bme1580I G_kGCm'C Cuts at: 1790 2437 3395 3525 4482 5638 1790 Size: 647 958 130 957 1156 2573 Fragments arranged by size: 2573 1156 958 957 647 130 BmgBI CAC'GTC Cuts at: 1318 2323 1318 Size: 1005 5416 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4152 5110 442 Size: 416 53 83 399 24 2735 958 1753 Fragments arranged by size: 2735 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6421 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5081 1239 Size: 3842 2579 Bpu10I CC'TnA_GC Cuts at: 2628 2628 Size: 6421 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4546 5411 5709 4546 Size: 865 298 5258 Fragments arranged by size: 5258 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2333 2825 5084 882 Size: 617 834 492 2259 2219 Fragments arranged by size: 2259 2219 834 617 492 BsaAI yAC'GTr Cuts at: 493 1204 2253 2416 3718 4212 493 Size: 711 1049 163 1302 494 2702 Fragments arranged by size: 2702 1302 1049 711 494 163 BsaBI GATnn'nnATC Cuts at: 2054 6415 2054 Size: 4361 2060 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 3372 4228 4610 Size: 53 83 186 936 570 1269 856 382 Cuts at: 4610 5245 275 Size: 635 1451 Fragments arranged by size: 1451 1269 936 856 635 570 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4854 5252 5526 5673 6084 4854 Size: 398 274 147 411 5191 Fragments arranged by size: 5191 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2055 2085 2899 2929 1178 Size: 30 165 30 652 30 814 30 4670 Fragments arranged by size: 4670 814 652 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2702 3120 3300 3492 3675 1186 Size: 1516 418 180 192 183 3932 Fragments arranged by size: 3932 1516 418 192 183 180 BseYI C'CCAG_C Cuts at: 5624 6120 5624 Size: 496 5925 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2071 2826 3095 1163 Size: 573 335 755 269 4489 Fragments arranged by size: 4489 755 573 335 269 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3871 4632 5660 1540 Size: 275 186 1870 761 1028 2301 Fragments arranged by size: 2301 1870 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2148 3525 4482 4567 5638 2148 Size: 1377 957 85 1071 2931 Fragments arranged by size: 2931 1377 1071 957 85 BsmI GAATG_Cn' Cuts at: 2251 3932 4025 2251 Size: 1681 93 4647 Fragments arranged by size: 4647 1681 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2437 3395 3525 3740 4482 4567 Size: 399 358 289 958 130 215 742 85 Cuts at: 4567 5638 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 958 742 399 358 289 215 130 85 BspEI T'CCGG_A Cuts at: 6084 6084 Size: 6421 BspHI T'CATG_A Cuts at: 2148 2241 2502 2952 4310 6040 2148 Size: 93 261 450 1358 1730 2529 Fragments arranged by size: 2529 1730 1358 450 261 93 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2122 3265 3776 844 Size: 1278 1143 511 3489 Fragments arranged by size: 3489 1278 1143 511 BsrBI CCG'CTC Cuts at: 1394 1823 4308 1394 Size: 429 2485 3507 Fragments arranged by size: 3507 2485 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2724 4916 5090 65 Size: 1183 1476 2192 174 1396 Fragments arranged by size: 2192 1476 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3000 3395 5065 5252 1700 Size: 110 1190 395 1670 187 2869 Fragments arranged by size: 2869 1670 1190 395 187 110 BsrGI T'GTAC_A Cuts at: 96 2520 96 Size: 2424 3997 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6421 BssSI C'ACGA_G Cuts at: 2093 4481 5493 2093 Size: 2388 1012 3021 Fragments arranged by size: 3021 2388 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6421 BstBI TT'CG_AA Cuts at: 2007 5225 2007 Size: 3218 3203 BstEII G'GTnAC_C Cuts at: 1184 3334 1184 Size: 2150 4271 BstXI CCAn_nnnn'nTGG Cuts at: 2257 2257 Size: 6421 BstYI r'GATC_y Cuts at: 1972 2049 3051 3675 3751 4118 4503 4520 5972 Size: 77 1002 624 76 367 385 17 1452 Cuts at: 5972 6058 6070 6416 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1002 624 385 367 346 86 77 76 17 12 Bsu36I CC'TnA_GG Cuts at: 3413 3413 Size: 6421 BtgI C'CryG_G Cuts at: 513 1065 2272 3319 513 Size: 552 1207 1047 3615 Fragments arranged by size: 3615 1207 1047 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5682 Fragments arranged by size: 5682 683 56 BtsI GCAGTG_nn' Cuts at: 2838 3937 4731 4751 2838 Size: 1099 794 20 4508 Fragments arranged by size: 4508 1099 794 20 ClaI AT'CG_AT Cuts at: 1554 3642 1554 Size: 2088 4333 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6386 DraI TTT'AAA Cuts at: 3468 3725 3468 Size: 257 6164 DraIII CAC_nnn'GTG Cuts at: 2430 3325 2430 Size: 895 5526 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2390 5428 809 Size: 507 1074 3038 1802 Fragments arranged by size: 3038 1802 1074 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2317 2379 2616 Size: 54 1425 50 275 18 487 62 237 Cuts at: 2616 2964 3144 3868 4757 6386 8 Size: 348 180 724 889 1629 43 Fragments arranged by size: 1629 1425 889 724 487 348 275 237 180 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3868 1537 Size: 275 2056 4090 Fragments arranged by size: 4090 2056 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4351 1353 Size: 2998 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2843 5008 5382 5528 387 Size: 1064 138 1254 2165 374 146 1280 Fragments arranged by size: 2165 1280 1254 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2146 2146 Size: 6421 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2721 3604 3651 4484 5081 5868 6136 Size: 393 1089 883 47 833 597 787 268 Cuts at: 6136 1239 Size: 1524 Fragments arranged by size: 1524 1089 883 833 787 597 393 268 47 EcoNI CCTnn'n_nnAGG Cuts at: 2057 3690 2057 Size: 1633 4788 EcoO109I rG'GnC_Cy Cuts at: 2515 3238 3417 3601 2515 Size: 723 179 184 5335 Fragments arranged by size: 5335 723 184 179 EcoRI G'AATT_C Cuts at: 3730 4108 3730 Size: 378 6043 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3058 3058 Size: 6421 FspI TGC'GCA Cuts at: 4927 4927 Size: 6421 HaeII r_GCGC'y Cuts at: 1678 5568 1678 Size: 3890 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2665 2697 2728 2760 3667 Size: 32 135 32 1093 32 31 32 907 Cuts at: 3667 3699 5062 5094 5136 5168 1373 Size: 32 1363 32 42 32 2626 Fragments arranged by size: 2626 1363 1093 907 135 42 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 2485 2761 3765 4011 669 Size: 988 281 547 276 1004 246 3079 Fragments arranged by size: 3079 1004 988 547 281 276 246 HindIII A'AGCT_T Cuts at: 748 3170 748 Size: 2422 3999 HpaI GTT'AAC Cuts at: 4011 4011 Size: 6421 KpnI G_GTAC'C Cuts at: 3746 3746 Size: 6421 MfeI C'AATT_G Cuts at: 4020 4020 Size: 6421 MluI A'CGCG_T Cuts at: 6297 6297 Size: 6421 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2275 3094 3183 5535 5719 1305 Size: 970 819 89 2352 184 2007 Fragments arranged by size: 2352 2007 970 819 184 89 MscI TGG'CCA Cuts at: 10 64 1489 1832 2966 10 Size: 54 1425 343 1134 3465 Fragments arranged by size: 3465 1425 1134 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 2306 4379 4738 4897 518 Size: 648 1089 51 2073 359 159 2042 Fragments arranged by size: 2073 2042 1089 648 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2798 3837 4514 5662 5907 1606 Size: 1192 1039 677 1148 245 2120 Fragments arranged by size: 2120 1192 1148 1039 677 245 NcoI C'CATG_G Cuts at: 513 1065 2272 513 Size: 552 1207 4662 Fragments arranged by size: 4662 1207 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6421 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6421 NotI GC'GGCC_GC Cuts at: 3868 3868 Size: 6421 NspI r_CATG'y Cuts at: 1132 1229 2527 2849 3313 3779 5324 1132 Size: 97 1298 322 464 466 1545 2229 Fragments arranged by size: 2229 1545 1298 466 464 322 97 PasI CC'CwG_GG Cuts at: 1348 3527 1348 Size: 2179 4242 PciI A'CATG_T Cuts at: 5320 5320 Size: 6421 PflMI CCAn_nnn'nTGG Cuts at: 1108 2134 1108 Size: 1026 5395 PfoI T'CCnGG_A Cuts at: 3208 3688 3208 Size: 480 5941 PmeI GTTT'AAAC Cuts at: 3725 3725 Size: 6421 PmlI CAC'GTG Cuts at: 2253 2416 2253 Size: 163 6258 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4490 4522 6003 6035 4490 Size: 32 1481 32 4876 Fragments arranged by size: 4876 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2515 3238 3417 3601 2515 Size: 723 179 184 5335 Fragments arranged by size: 5335 723 184 179 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6421 PsiI TTA'TAA Cuts at: 3991 3991 Size: 6421 PstI C_TGCA'G Cuts at: 830 3773 830 Size: 2943 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6421 PvuII CAG'CTG Cuts at: 2798 2798 Size: 6421 SacI G_AGCT'C Cuts at: 2148 2148 Size: 6421 SalI G'TCGA_C Cuts at: 1936 3763 1936 Size: 1827 4594 SanDI GG'GwC_CC Cuts at: 3238 3238 Size: 6421 SbfI CC_TGCA'GG Cuts at: 3773 3773 Size: 6421 ScaI AGT'ACT Cuts at: 4669 6381 4669 Size: 1712 4709 SexAI A'CCwGG_T Cuts at: 2426 2754 2426 Size: 328 6093 SfcI C'TryA_G Cuts at: 826 1045 3769 4904 5585 5776 826 Size: 219 2724 1135 681 191 1471 Fragments arranged by size: 2724 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6421 SmaI CCC'GGG Cuts at: 1872 3748 1872 Size: 1876 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4525 5426 5688 820 Size: 197 926 2582 901 262 1553 Fragments arranged by size: 2582 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3718 4212 493 Size: 3225 494 2702 Fragments arranged by size: 3225 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6421 SphI G_CATG'C Cuts at: 1132 3779 1132 Size: 2647 3774 SspI AAT'ATT Cuts at: 5 52 4345 5 Size: 47 4293 2081 Fragments arranged by size: 4293 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6421 StyI C'CwwG_G Cuts at: 513 1065 2166 2272 2280 2388 2439 3860 513 Size: 552 1101 106 8 108 51 1421 3074 Fragments arranged by size: 3074 1421 1101 552 108 106 51 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2105 2237 2452 4448 4465 4618 4803 Size: 1255 525 132 215 1996 17 153 185 Cuts at: 4803 5248 325 Size: 445 1498 Fragments arranged by size: 1996 1498 1255 525 445 215 185 153 132 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2520 4667 6379 Size: 274 80 33 51 1443 543 2147 1712 Cuts at: 6379 96 Size: 138 Fragments arranged by size: 2147 1712 1443 543 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2055 2087 2178 2210 2899 2931 6381 6413 2055 Size: 32 91 32 689 32 3450 32 2063 Fragments arranged by size: 3450 2063 689 91 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3431 1316 Size: 2115 4306 XbaI T'CTAG_A Cuts at: 3757 3757 Size: 6421 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2188 2600 2188 Size: 412 6009 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6421 XmaI C'CCGG_G Cuts at: 1870 3746 1870 Size: 1876 4545 XmnI GAAnn'nnTTC Cuts at: 4550 4550 Size: 6421 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2104 4229 276 Size: 53 83 186 936 570 2125 2468 Fragments arranged by size: 2468 2125 936 570 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI