Restriction map for
pFN21ASDB0018
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII AcsI AflII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
BalI BcgI BclI BfrI BglII
BlpI Bpu1102I BsiEI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FbaI FseI
HincII HindII HindIII HpaI KspI
MfeI MluI MluNI MscI MunI
NaeI NdeI NgoMIV NheI NotI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI PvuI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 316 GGTACC G^GTACC
AccI 1903 GTCTAC GT^[AC][GT]AC
AccII 127 CGCG CG^CG
AclI 208 AACGTT AA^CGTT
AflIII 759 ACGTGT A^C[GA][CT]GT
ApaI 2104 GGGCCC GGGCC^C
Asp718I 316 GGTACC G^GTACC
AspI 334 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 419 TGCGCA TGC^GCA
AvrII 39 CCTAGG C^CTAGG
BamHI 734 GGATCC G^GATCC
BbrPI 651 CACGTG CAC^GTG
BciVI 59 GTATCC GTATCC(6/5)
BlnI 39 CCTAGG C^CTAGG
BsmBI 400 GAGACG CGTCTC(1/5)
BspHI 1263 TCATGA T^CATGA
BsrDI 296 GCAATG GCAATG(2/0)
BssSI 502 CTCGTG CACGAG(-5/-1)
BstUI 127 CGCG CG^CG
DrdI 1693 GACATCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 671 CCATGG C^C[GA][CT]GG
EcoRV 1347 GATATC GAT^ATC
FspI 419 TGCGCA TGC^GCA
KpnI 316 GGTACC GGTAC^C
MvnI 127 CGCG CG^CG
NcoI 671 CCATGG C^CATGG
NruI 126 TCGCGA TCG^CGA
NspI 2257 GCATGT [GA]CATG^[CT]
PflFI 334 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 651 CACGTG CAC^GTG
PmlI 651 CACGTG CAC^GTG
Psp1406I 208 AACGTT AA^CGTT
PstI 166 CTGCAG CTGCA^G
RcaI 1263 TCATGA T^CATGA
SbfI 165 CCTGCAGG CCTGCA^GG
Sse8387I 165 CCTGCAGG CCTGCA^GG
Tth111I 334 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1961 CCATCCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AvaI 278 CCCGGG C^[CT]CG[GA]G
2483 CCCGGG
BanII 945 GAGCCC G[GA]GC[CT]^C
2104 GGGCCC
BbeI 492 GGCGCC GGCGC^C
590 GGCGCC
BbvCI 241 GCTGAGG CCTCAGC(-5/-2)
996 GCTGAGG
BfaI 40 CTAG C^TAG
1843 CTAG
BsaAI 651 CACGTG [CT]AC^GT[GA]
758 TACGTG
BsaBI 372 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1506 GATCCCCATC
BsoBI 278 CCCGGG C^[CT]CG[GA]G
2483 CCCGGG
BspMI 249 ACCTGC ACCTGC(4/8)
168 GCAGGT
BsrBI 936 CCGCTC CCGCTC(-3/-3)
2546 CCGCTC
BstXI 1061 CCAGAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2387 CCAGGCCACTGG
BstYI 734 GGATCC [GA]^GATC[CT]
1505 AGATCC
EarI 981 CTCTTC CTCTTC(1/4)
1813 GAAGAG
HphI 706 GGTGA GGTGA(8/7)
2506 GGTGA
KasI 492 GGCGCC G^GCGCC
590 GGCGCC
Ksp632I 981 CTCTTC CTCTTC(1/4)
1813 GAAGAG
MaeI 40 CTAG C^TAG
1843 CTAG
MamI 372 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1506 GATCCCCATC
MflI 734 GGATCC [GA]^GATC[CT]
1505 AGATCC
MseI 1997 TTAA T^TAA
2600 TTAA
MslI 498 CACACTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2139 CACCCACATG
NarI 492 GGCGCC GG^CGCC
590 GGCGCC
SfaNI 377 GCATC GCATC(5/9)
2479 GCATC
SfoI 492 GGCGCC GGC^GCC
590 GGCGCC
SmaI 278 CCCGGG CCC^GGG
2483 CCCGGG
StuI 1494 AGGCCT AGG^CCT
2359 AGGCCT
Tru9I 1997 TTAA T^TAA
2600 TTAA
XhoII 734 GGATCC [GA]^GATC[CT]
1505 AGATCC
XmaCI 278 CCCGGG C^CCGGG
2483 CCCGGG
XmaI 278 CCCGGG C^CCGGG
2483 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccIII AciI AcyI AfaI AluI
AlwI AlwNI AocI AvaII BanI
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspEI
BsrI BssKI BstF5I BstNI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI MaeII
MaeIII MboI MboII MnlI MroI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I