Restriction map for
pFN21ASDB0018
(Linear) (Six-base) MAPSORT of: KIBB0018.seq Check: 1367 from: 1 to: 2640
KIBB0018.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 159 258 2640
Size: 159 99 2382
Fragments arranged by size:
2382 159 99
AccI GT'mk_AC
Cuts at: 0 1904 2640
Size: 1904 736
Acc65I G'GTAC_C
Cuts at: 0 316 2640
Size: 316 2324
AclI AA'CG_TT
Cuts at: 0 209 2640
Size: 209 2431
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 968 2341 2483 2640
Size: 874 94 1373 142 157
Fragments arranged by size:
1373 874 157 142 94
AflIII A'CryG_T
Cuts at: 0 759 2640
Size: 759 1881
AleI CACnn'nnGTG
Cuts at: 0 502 2640
Size: 502 2138
AlwNI CAG_nnn'CTG
Cuts at: 0 90 174 1468 1689 2640
Size: 90 84 1294 221 951
Fragments arranged by size:
1294 951 221 90 84
ApaI G_GGCC'C
Cuts at: 0 2108 2640
Size: 2108 532
AvaI C'yCGr_G
Cuts at: 0 278 2483 2640
Size: 278 2205 157
Fragments arranged by size:
2205 278 157
AvrII C'CTAG_G
Cuts at: 0 39 2640
Size: 39 2601
BamHI G'GATC_C
Cuts at: 0 734 2640
Size: 734 1906
BanI G'GyrC_C
Cuts at: 0 246 316 492 590 1071 1085 2137 2640
Size: 246 70 176 98 481 14 1052 503
Fragments arranged by size:
1052 503 481 246 176 98 70 14
BanII G_rGCy'C
Cuts at: 0 949 2108 2640
Size: 949 1159 532
Fragments arranged by size:
1159 949 532
BbeI G_GCGC'C
Cuts at: 0 496 594 2640
Size: 496 98 2046
Fragments arranged by size:
2046 496 98
BbsI GAAGACnn'nnnn_
Cuts at: 0 113 976 2397 2640
Size: 113 863 1421 243
Fragments arranged by size:
1421 863 243 113
BbvCI CC'TCA_GC
Cuts at: 0 242 997 2640
Size: 242 755 1643
Fragments arranged by size:
1643 755 242
BciVI GTATCCnnnnn_n'
Cuts at: 0 70 2640
Size: 70 2570
BglI GCCn_nnn'nGGC
Cuts at: 0 238 588 1330 2020 2640
Size: 238 350 742 690 620
Fragments arranged by size:
742 690 620 350 238
Bme1580I G_kGCm'C
Cuts at: 0 658 1873 2108 2140 2640
Size: 658 1215 235 32 500
Fragments arranged by size:
1215 658 500 235 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 652 793 1146 2640
Size: 652 141 353 1494
Fragments arranged by size:
1494 652 353 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 475 532 1044 2558 2640
Size: 112 363 57 512 1514 82
Fragments arranged by size:
1514 512 363 112 82 57
Bpu10I CC'TnA_GC
Cuts at: 0 242 997 1934 1976 2042 2640
Size: 242 755 937 42 66 598
Fragments arranged by size:
937 755 598 242 66 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 294 1103 1172 1592 2322 2345 2640
Size: 294 809 69 420 730 23 295
Fragments arranged by size:
809 730 420 295 294 69 23
BsaAI yAC'GTr
Cuts at: 0 653 760 2640
Size: 653 107 1880
Fragments arranged by size:
1880 653 107
BsaBI GATnn'nnATC
Cuts at: 0 376 1510 2640
Size: 376 1134 1130
Fragments arranged by size:
1134 1130 376
BsaHI Gr'CG_yC
Cuts at: 0 493 591 1292 2640
Size: 493 98 701 1348
Fragments arranged by size:
1348 701 493 98
BsaWI w'CCGG_w
Cuts at: 0 731 740 2285 2640
Size: 731 9 1545 355
Fragments arranged by size:
1545 731 355 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1301 1331 1450 1480 1741 1771 1845 1875
Size: 1301 30 119 30 261 30 74 30
Cuts at: 1875 2486 2516 2640
Size: 611 30 124
Fragments arranged by size:
1301 611 261 124 119 74 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 529 532 541 544 1225 1831 2524 2527
Size: 529 3 9 3 681 606 693 3
Cuts at: 2527 2530 2545 2638 2640
Size: 3 15 93 2
Fragments arranged by size:
693 681 606 529 93 15 9 3
3 3 3 2
BseYI C'CCAG_C
Cuts at: 0 5 308 459 773 914 1129 1144 1280
Size: 5 303 151 314 141 215 15 136
Cuts at: 1280 1389 1636 1921 2457 2640
Size: 109 247 285 536 183
Fragments arranged by size:
536 314 303 285 247 215 183 151
141 136 109 15 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 526 633 649 682 804 895 2640
Size: 75 451 107 16 33 122 91 1745
Fragments arranged by size:
1745 451 122 107 91 75 33 16
BsmBI CGTCTCn'nnnn_
Cuts at: 0 394 2640
Size: 394 2246
Bsp1286I G_dGCh'C
Cuts at: 0 658 949 1873 2108 2140 2640
Size: 658 291 924 235 32 500
Fragments arranged by size:
924 658 500 291 235 32
BspEI T'CCGG_A
Cuts at: 0 731 740 2285 2640
Size: 731 9 1545 355
Fragments arranged by size:
1545 731 355 9
BspHI T'CATG_A
Cuts at: 0 1263 2640
Size: 1263 1377
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 159 258 2640
Size: 159 99 2382
Fragments arranged by size:
2382 159 99
BsrBI CCG'CTC
Cuts at: 0 938 2548 2640
Size: 938 1610 92
Fragments arranged by size:
1610 938 92
BsrDI GCAATG_nn'
Cuts at: 0 303 2640
Size: 303 2337
BssSI C'ACGA_G
Cuts at: 0 502 2640
Size: 502 2138
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1068 2394 2640
Size: 1068 1326 246
Fragments arranged by size:
1326 1068 246
BstYI r'GATC_y
Cuts at: 0 734 1505 2640
Size: 734 771 1135
Fragments arranged by size:
1135 771 734
Bsu36I CC'TnA_GG
Cuts at: 0 1081 1408 2162 2621 2640
Size: 1081 327 754 459 19
Fragments arranged by size:
1081 754 459 327 19
BtgI C'CryG_G
Cuts at: 0 671 2640
Size: 671 1969
BtsI GCAGTG_nn'
Cuts at: 0 645 1902 1933 2640
Size: 645 1257 31 707
Fragments arranged by size:
1257 707 645 31
DrdI GACnn_nn'nnGTC
Cuts at: 0 1699 2640
Size: 1699 941
EarI CTCTTCn'nnn_
Cuts at: 0 987 1808 2640
Size: 987 821 832
Fragments arranged by size:
987 832 821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 475 532 874 968 1044 2341 2483
Size: 112 363 57 342 94 76 1297 142
Cuts at: 2483 2558 2640
Size: 75 82
Fragments arranged by size:
1297 363 342 142 112 94 82 76
75 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 14 241 1490 1813 2165 2640
Size: 14 227 1249 323 352 475
Fragments arranged by size:
1249 475 352 323 227 14
EcoO109I rG'GnC_Cy
Cuts at: 0 35 1001 1055 1728 1945 1982 2154 2211
Size: 35 966 54 673 217 37 172 57
Cuts at: 2211 2301 2580 2640
Size: 90 279 60
Fragments arranged by size:
966 673 279 217 172 90 60 57
54 37 35
EcoRV GAT'ATC
Cuts at: 0 1349 2640
Size: 1349 1291
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2629 2640
Size: 2629 11
FspI TGC'GCA
Cuts at: 0 421 2640
Size: 421 2219
HaeII r_GCGC'y
Cuts at: 0 480 496 583 594 2640
Size: 480 16 87 11 2046
Fragments arranged by size:
2046 480 87 16 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 325 357 889 921 1450 1482 1589 1621
Size: 325 32 532 32 529 32 107 32
Cuts at: 1621 1845 1877 2224 2256 2614 2640
Size: 224 32 347 32 358 26
Fragments arranged by size:
532 529 358 347 325 224 107 32
32 32 32 32 32 26
KasI G'GCGC_C
Cuts at: 0 492 590 2640
Size: 492 98 2050
Fragments arranged by size:
2050 492 98
KpnI G_GTAC'C
Cuts at: 0 320 2640
Size: 320 2320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 1504 2640
Size: 737 767 1136
Fragments arranged by size:
1136 767 737
MslI CAynn'nnrTG
Cuts at: 0 502 2143 2640
Size: 502 1641 497
Fragments arranged by size:
1641 502 497
MspA1I CmG'CkG
Cuts at: 0 510 818 1129 1280 1689 2050 2640
Size: 510 308 311 151 409 361 590
Fragments arranged by size:
590 510 409 361 311 308 151
NarI GG'CG_CC
Cuts at: 0 493 591 2640
Size: 493 98 2049
Fragments arranged by size:
2049 493 98
NcoI C'CATG_G
Cuts at: 0 671 2640
Size: 671 1969
NruI TCG'CGA
Cuts at: 0 128 2640
Size: 128 2512
NspI r_CATG'y
Cuts at: 0 2261 2640
Size: 2261 379
PasI CC'CwG_GG
Cuts at: 0 1235 2640
Size: 1235 1405
PflMI CCAn_nnn'nTGG
Cuts at: 0 753 795 1256 1689 1704 1971 2640
Size: 753 42 461 433 15 267 669
Fragments arranged by size:
753 669 461 433 267 42 15
PfoI T'CCnGG_A
Cuts at: 0 1421 2640
Size: 1421 1219
PmlI CAC'GTG
Cuts at: 0 653 2640
Size: 653 1987
PpuMI rG'GwC_Cy
Cuts at: 0 35 1728 1945 1982 2154 2301 2640
Size: 35 1693 217 37 172 147 339
Fragments arranged by size:
1693 339 217 172 147 37 35
PspOMI G'GGCC_C
Cuts at: 0 2104 2640
Size: 2104 536
PstI C_TGCA'G
Cuts at: 0 170 2640
Size: 170 2470
PvuII CAG'CTG
Cuts at: 0 510 1129 1280 1689 2050 2640
Size: 510 619 151 409 361 590
Fragments arranged by size:
619 590 510 409 361 151
SanDI GG'GwC_CC
Cuts at: 0 1982 2301 2640
Size: 1982 319 339
Fragments arranged by size:
1982 339 319
SbfI CC_TGCA'GG
Cuts at: 0 170 2640
Size: 170 2470
SfcI C'TryA_G
Cuts at: 0 166 1738 1803 2640
Size: 166 1572 65 837
Fragments arranged by size:
1572 837 166 65
SfoI GGC'GCC
Cuts at: 0 494 592 2640
Size: 494 98 2048
Fragments arranged by size:
2048 494 98
SmaI CCC'GGG
Cuts at: 0 280 2485 2640
Size: 280 2205 155
Fragments arranged by size:
2205 280 155
StuI AGG'CCT
Cuts at: 0 1496 2361 2640
Size: 1496 865 279
Fragments arranged by size:
1496 865 279
StyI C'CwwG_G
Cuts at: 0 39 671 1433 1491 1772 2132 2228 2252
Size: 39 632 762 58 281 360 96 24
Cuts at: 2252 2432 2640
Size: 180 208
Fragments arranged by size:
762 632 360 281 208 180 96 58
39 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 694 1004 1158 2155 2640
Size: 694 310 154 997 485
Fragments arranged by size:
997 694 485 310 154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 684 716 1301 1333 2640
Size: 684 32 585 32 1307
Fragments arranged by size:
1307 684 585 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 337 2640
Size: 337 2303
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1968 2640
Size: 1968 672
XmaI C'CCGG_G
Cuts at: 0 278 2483 2640
Size: 278 2205 157
Fragments arranged by size:
2205 278 157
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BciVI BglI Bme1580I BmrI BpmI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BssSI
BstXI BstYI Bsu36I BtgI BtsI DrdI EarI Eco57MI
EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I KasI
KpnI MmeI MslI MspA1I NarI NcoI NruI NspI
PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SanDI SbfI SfcI SfoI SmaI StuI StyI TaqII
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaLI
ApoI AscI AseI AsiSI BaeI BcgI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BpuEI BsiEI BsiHKAI
BsiWI BsmI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraI DraIII EaeI EagI
EciI EcoICRI EcoRI FseI FspAI HincII HindIII HpaI
MfeI MluI MscI NaeI NdeI NgoMIV NheI NotI
NsiI PacI PciI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TatI XbaI XhoI XmnI ZraI