(Circular) (Six-base) MAPSORT of: pFN21ASDA1934.seq Check: 6767 from: 1 to: 6073 pFN21ASDA1934 6073 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3883 278 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2335 3082 3416 1937 Size: 398 747 334 4594 Fragments arranged by size: 4594 747 398 334 Acc65I G'GTAC_C Cuts at: 3394 3394 Size: 6073 AclI AA'CG_TT Cuts at: 1563 4200 4573 1563 Size: 2637 373 3063 Fragments arranged by size: 3063 2637 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4136 5520 5788 1632 Size: 2504 1384 268 1917 Fragments arranged by size: 2504 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5876 AflIII A'CryG_T Cuts at: 1203 4972 5949 1203 Size: 3769 977 1327 Fragments arranged by size: 3769 1327 977 AgeI A'CCGG_T Cuts at: 4904 4904 Size: 6073 AhdI GACnn_n'nnGTC Cuts at: 4802 4802 Size: 6073 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6073 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6073 AlwNI CAG_nnn'CTG Cuts at: 2158 3332 3816 5388 2158 Size: 1174 484 1572 2843 Fragments arranged by size: 2843 1572 1174 484 ApaLI G'TGCA_C Cuts at: 2843 4130 5286 2843 Size: 1287 1156 3630 Fragments arranged by size: 3630 1287 1156 ApoI r'AATT_y Cuts at: 1493 2124 3382 3608 3760 5869 1493 Size: 631 1258 226 152 2109 1697 Fragments arranged by size: 2109 1697 1258 631 226 152 AseI AT'TA_AT Cuts at: 160 1032 4627 160 Size: 872 3595 1606 Fragments arranged by size: 3595 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 AvaI C'yCGr_G Cuts at: 1870 1943 2904 3398 1870 Size: 73 961 494 4545 Fragments arranged by size: 4545 961 494 73 AvrII C'CTAG_G Cuts at: 2176 2176 Size: 6073 BamHI G'GATC_C Cuts at: 3403 3770 3403 Size: 367 5706 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3394 4849 618 Size: 325 217 627 1607 1455 1842 Fragments arranged by size: 1842 1607 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2392 2826 2855 2946 3392 1391 Size: 1001 434 29 91 446 4072 Fragments arranged by size: 4072 1001 446 434 91 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2205 2209 2556 3240 928 Size: 971 306 4 347 684 3761 Fragments arranged by size: 3761 971 684 347 306 4 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4264 4298 1903 Size: 34 2327 34 3678 Fragments arranged by size: 3678 2327 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3264 3965 5175 3264 Size: 701 1210 4162 Fragments arranged by size: 4162 1210 701 BclI T'GATC_A Cuts at: 1548 1548 Size: 6073 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2587 4684 136 Size: 107 122 71 631 605 915 2097 1525 Fragments arranged by size: 2097 1525 915 631 605 122 107 71 BglII A'GATC_T Cuts at: 2721 6068 2721 Size: 3347 2726 BlpI GC'TnA_GC Cuts at: 3490 3490 Size: 6073 Bme1580I G_kGCm'C Cuts at: 1790 2847 4134 5290 1790 Size: 1057 1287 1156 2573 Fragments arranged by size: 2573 1287 1156 1057 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6073 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2253 3804 4762 Size: 416 53 83 399 24 836 1551 958 Cuts at: 4762 442 Size: 1753 Fragments arranged by size: 1753 1551 958 836 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6073 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2815 2815 Size: 6073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2645 2651 2960 2999 3261 4733 1239 Size: 1406 6 309 39 262 1472 2579 Fragments arranged by size: 2579 1472 1406 309 262 39 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4198 5063 5361 4198 Size: 865 298 4910 Fragments arranged by size: 4910 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2534 4736 882 Size: 617 1035 2202 2219 Fragments arranged by size: 2219 2202 1035 617 BsaAI yAC'GTr Cuts at: 493 1204 2636 3252 3370 3864 493 Size: 711 1432 616 118 494 2702 Fragments arranged by size: 2702 1432 711 616 494 118 BsaBI GATnn'nnATC Cuts at: 6067 6067 Size: 6073 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2709 3880 4262 4897 Size: 53 83 186 936 1176 1171 382 635 Cuts at: 4897 275 Size: 1451 Fragments arranged by size: 1451 1176 1171 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3077 4506 4904 5178 5325 5736 3077 Size: 1429 398 274 147 411 3414 Fragments arranged by size: 3414 1429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2666 2696 2781 2811 1178 Size: 30 165 30 1263 30 85 30 4440 Fragments arranged by size: 4440 1263 165 85 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3323 1186 Size: 2137 3936 BseYI C'CCAG_C Cuts at: 3332 5276 5772 3332 Size: 1944 496 3633 Fragments arranged by size: 3633 1944 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3096 3149 1163 Size: 573 1360 53 4087 Fragments arranged by size: 4087 1360 573 53 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3523 4284 5312 1540 Size: 275 186 1522 761 1028 2301 Fragments arranged by size: 2301 1522 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2667 2847 2946 4134 4219 5290 2667 Size: 180 99 1188 85 1071 3450 Fragments arranged by size: 3450 1188 1071 180 99 85 BsmI GAATG_Cn' Cuts at: 3584 3677 3584 Size: 93 5980 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2392 2667 2826 2847 2855 2946 3392 Size: 399 602 275 159 21 8 91 446 Cuts at: 3392 4134 4219 5290 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 602 446 399 275 159 91 85 21 8 BspEI T'CCGG_A Cuts at: 5736 5736 Size: 6073 BspHI T'CATG_A Cuts at: 3962 5692 3962 Size: 1730 4343 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2554 3428 844 Size: 1710 874 3489 Fragments arranged by size: 3489 1710 874 BsrBI CCG'CTC Cuts at: 1394 1823 3960 1394 Size: 429 2137 3507 Fragments arranged by size: 3507 2137 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4568 4742 65 Size: 1183 3320 174 1396 Fragments arranged by size: 3320 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4717 4904 1700 Size: 110 2907 187 2869 Fragments arranged by size: 2907 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6073 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6073 BssSI C'ACGA_G Cuts at: 4133 5145 4133 Size: 1012 5061 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6073 BstBI TT'CG_AA Cuts at: 2007 4877 2007 Size: 2870 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6073 BstXI CCAn_nnnn'nTGG Cuts at: 2392 2692 2977 2392 Size: 300 285 5488 Fragments arranged by size: 5488 300 285 BstYI r'GATC_y Cuts at: 1972 2450 2721 3403 3770 4155 4172 5624 5710 Size: 478 271 682 367 385 17 1452 86 Cuts at: 5710 5722 6068 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 682 478 385 367 346 271 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2927 3117 2927 Size: 190 5883 BtgI C'CryG_G Cuts at: 513 1065 2022 2103 3238 513 Size: 552 957 81 1135 3348 Fragments arranged by size: 3348 1135 957 552 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5334 Fragments arranged by size: 5334 683 56 BtsI GCAGTG_nn' Cuts at: 2033 2705 3036 3220 3589 4383 4403 2033 Size: 672 331 184 369 794 20 3703 Fragments arranged by size: 3703 794 672 369 331 184 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6073 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6038 DraI TTT'AAA Cuts at: 3377 3377 Size: 6073 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5080 809 Size: 507 3764 1802 Fragments arranged by size: 3764 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2382 3241 3520 Size: 54 1425 50 275 18 552 859 279 Cuts at: 3520 4409 6038 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 859 552 279 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3520 1537 Size: 275 1708 4090 Fragments arranged by size: 4090 1708 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2532 2603 3003 4003 1353 Size: 1179 71 400 1000 3423 Fragments arranged by size: 3423 1179 1000 400 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4660 5034 5180 387 Size: 1064 138 3071 374 146 1280 Fragments arranged by size: 3071 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2944 2944 Size: 6073 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2645 2651 2960 2999 3261 4136 4733 Size: 393 1013 6 309 39 262 875 597 Cuts at: 4733 5520 5788 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1013 875 787 597 393 309 268 262 39 6 EcoNI CCTnn'n_nnAGG Cuts at: 2529 2529 Size: 6073 EcoO109I rG'GnC_Cy Cuts at: 2080 2173 3072 2080 Size: 93 899 5081 Fragments arranged by size: 5081 899 93 EcoRI G'AATT_C Cuts at: 3382 3760 3382 Size: 378 5695 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2164 2233 2740 2872 2164 Size: 69 507 132 5365 Fragments arranged by size: 5365 507 132 69 FspI TGC'GCA Cuts at: 2164 2867 4579 2164 Size: 703 1712 3658 Fragments arranged by size: 3658 1712 703 FspAI rTGC'GCAy Cuts at: 2164 2164 Size: 6073 HaeII r_GCGC'y Cuts at: 1678 5220 1678 Size: 3542 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2781 2813 3038 3070 4714 Size: 32 135 32 1209 32 225 32 1644 Cuts at: 4714 4746 4788 4820 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1644 1209 225 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3417 3663 669 Size: 988 281 1479 246 3079 Fragments arranged by size: 3079 1479 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6073 HpaI GTT'AAC Cuts at: 3663 3663 Size: 6073 KpnI G_GTAC'C Cuts at: 3398 3398 Size: 6073 MfeI C'AATT_G Cuts at: 3672 3672 Size: 6073 MluI A'CGCG_T Cuts at: 5949 5949 Size: 6073 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5187 5371 1305 Size: 3882 184 2007 Fragments arranged by size: 3882 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2384 3243 10 Size: 54 1425 343 552 859 2840 Fragments arranged by size: 2840 1425 859 552 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2102 2660 2690 2876 4031 4390 4549 Size: 648 936 558 30 186 1155 359 159 Cuts at: 4549 518 Size: 2042 Fragments arranged by size: 2042 1155 936 648 558 359 186 159 30 MspA1I CmG'CkG Cuts at: 1606 3489 4166 5314 5559 1606 Size: 1883 677 1148 245 2120 Fragments arranged by size: 2120 1883 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2103 3238 513 Size: 552 957 81 1135 3348 Fragments arranged by size: 3348 1135 957 552 81 NdeI CA'TA_TG Cuts at: 387 2585 387 Size: 2198 3875 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6073 NotI GC'GGCC_GC Cuts at: 3520 3520 Size: 6073 NspI r_CATG'y Cuts at: 1132 1229 3431 4976 1132 Size: 97 2202 1545 2229 Fragments arranged by size: 2229 2202 1545 97 PasI CC'CwG_GG Cuts at: 1348 2392 3357 1348 Size: 1044 965 4064 Fragments arranged by size: 4064 1044 965 PciI A'CATG_T Cuts at: 4972 4972 Size: 6073 PflMI CCAn_nnn'nTGG Cuts at: 1108 3357 1108 Size: 2249 3824 PmeI GTTT'AAAC Cuts at: 3377 3377 Size: 6073 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4142 4174 5655 5687 4142 Size: 32 1481 32 4528 Fragments arranged by size: 4528 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2173 2173 Size: 6073 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6073 PsiI TTA'TAA Cuts at: 3643 3643 Size: 6073 PstI C_TGCA'G Cuts at: 830 2772 2835 3304 3425 830 Size: 1942 63 469 121 3478 Fragments arranged by size: 3478 1942 469 121 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6073 SacI G_AGCT'C Cuts at: 2946 2946 Size: 6073 SalI G'TCGA_C Cuts at: 1936 3415 1936 Size: 1479 4594 SbfI CC_TGCA'GG Cuts at: 3425 3425 Size: 6073 ScaI AGT'ACT Cuts at: 4321 6033 4321 Size: 1712 4361 SfcI C'TryA_G Cuts at: 826 1045 2768 2831 3300 3421 4556 5237 5428 Size: 219 1723 63 469 121 1135 681 191 Cuts at: 5428 826 Size: 1471 Fragments arranged by size: 1723 1471 1135 681 469 219 191 121 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6073 SmaI CCC'GGG Cuts at: 1872 3400 1872 Size: 1528 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4177 5078 5340 820 Size: 197 926 2234 901 262 1553 Fragments arranged by size: 2234 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3370 3864 493 Size: 2877 494 2702 Fragments arranged by size: 2877 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6073 SphI G_CATG'C Cuts at: 1132 3431 1132 Size: 2299 3774 SspI AAT'ATT Cuts at: 5 52 3997 5 Size: 47 3945 2081 Fragments arranged by size: 3945 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6073 StyI C'CwwG_G Cuts at: 513 1065 2022 2103 2176 2208 3238 3258 3512 Size: 552 957 81 73 32 1030 20 254 Cuts at: 3512 513 Size: 3074 Fragments arranged by size: 3074 1030 957 552 254 81 73 32 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4100 4117 4270 4455 4900 325 Size: 1255 2520 17 153 185 445 1498 Fragments arranged by size: 2520 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4319 6031 96 Size: 274 80 33 51 1443 2342 1712 138 Fragments arranged by size: 2342 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6033 6065 6033 Size: 32 6041 Tth111I GACn'n_nGTC Cuts at: 1316 2237 1316 Size: 921 5152 XbaI T'CTAG_A Cuts at: 3409 3409 Size: 6073 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6073 XmaI C'CCGG_G Cuts at: 1870 3398 1870 Size: 1528 4545 XmnI GAAnn'nnTTC Cuts at: 2345 2518 4202 2345 Size: 173 1684 4216 Fragments arranged by size: 4216 1684 173 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3881 276 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI