Restriction map for
pFN21ASDA1820
##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccI AclI AcsI AflII
ApoI AscI AseI AsnI Asp700I
AspI AviII AvrII BbrPI BciVI
BclI BfrI BlnI BsaAI BsaBI
BsiWI BsmBI Bsp1407I BspDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI MamI MfeI
MluI MslI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SapI SfiI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 983 TCCGGA T^CCGGA
AflIII 1688 ACATGT A^C[GA][CT]GT
AgeI 2504 ACCGGT A^CCGGT
AocI 3787 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3422 AGCGCT AGC^GCT
BamHI 4704 GGATCC G^GATCC
BfaI 79 CTAG C^TAG
BglII 3461 AGATCT A^GATCT
BseAI 983 TCCGGA T^CCGGA
BsmI 633 GCATTC GAATGC(1/-1)
BspEI 983 TCCGGA T^CCGGA
BspHI 4235 TCATGA T^CATGA
BspLU11I 1688 ACATGT A^CATGT
BssSI 1402 CTCGTG CACGAG(-5/-1)
Bsu36I 3787 CCTGAGG CC^T[ACGT]AGG
DrdI 973 GACGCAGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3335 CGGCCG C^GGCCG
EclXI 3335 CGGCCG C^GGCCG
Eco47III 3422 AGCGCT AGC^GCT
Eco52I 3335 CGGCCG C^GGCCG
Eco81I 3787 CCTGAGG CC^T[ACGT]AGG
HincII 1624 GTCAAC GT[CT]^[GA]AC
HindII 1624 GTCAAC GT[CT]^[GA]AC
MaeI 79 CTAG C^TAG
MaeII 515 ACGT A^CGT
MroI 983 TCCGGA T^CCGGA
MseI 4222 TTAA T^TAA
PflMI 4174 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2504 ACCGGT A^CCGGT
RcaI 4235 TCATGA T^CATGA
Tru9I 4222 TTAA T^TAA
Tsp509I 85 AATT ^AATT
Van91I 4174 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 616 GGTACC G^GTACC
2125 GGTACC
Alw44I 3007 GTGCAC G^TGCAC
4869 GTGCAC
ApaLI 3007 GTGCAC G^TGCAC
4869 GTGCAC
Asp718I 616 GGTACC G^GTACC
2125 GGTACC
BcgI 4861 CGACCGTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3 GCAGTCTTTTCG
BsaI 3886 GAGACC GGTCTC(1/5)
3564 GGTCTC
BsaWI 983 TCCGGA [AT]^CCGG[AT]
2504 ACCGGT
HindIII 1871 AAGCTT A^AGCTT
2988 AAGCTT
KpnI 616 GGTACC GGTAC^C
2125 GGTACC
KspI 212 CCGCGG CCGC^GG
1783 CCGCGG
NcoI 30 CCATGG C^CATGG
4365 CCATGG
PvuII 1351 CAGCTG CAG^CTG
2014 CAGCTG
SacI 2626 GAGCTC GAGCT^C
2879 GAGCTC
SacII 212 CCGCGG CCGC^GG
1783 CCGCGG
SbfI 270 CCTGCAGG CCTGCA^GG
403 CCTGCAGG
ScaI 998 AGTACT AGT^ACT
2609 AGTACT
SexAI 3621 ACCTGGT A^CC[AT]GGT
3713 ACCTGGT
SmaI 774 CCCGGG CCC^GGG
2419 CCCGGG
SphI 1524 GCATGC GCATG^C
2657 GCATGC
Sse8387I 270 CCTGCAGG CCTGCA^GG
403 CCTGCAGG
XmaCI 774 CCCGGG C^CCGGG
2419 CCCGGG
XmaI 774 CCCGGG C^CCGGG
2419 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI ApaI AspEI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I XcmI XhoII