Restriction map for
pFN21ASDA1820
(Circular) (Six-base) MAPSORT of: pFN21ASDA1820.seq Check: 3347 from: 1 to: 9649
pFN21ASDA1820 9649 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4478 6703 4478
Size: 2225 7424
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7459 278
Size: 53 83 186 936 5923 2468
Fragments arranged by size:
5923 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6992 1937
Size: 5055 4594
Acc65I G'GTAC_C
Cuts at: 2639 4148 6970 2639
Size: 1509 2822 5318
Fragments arranged by size:
5318 2822 1509
AclI AA'CG_TT
Cuts at: 1563 7776 8149 1563
Size: 6213 373 3063
Fragments arranged by size:
6213 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2360 2885 3019 3257 3924 4051 6023 7712
Size: 728 525 134 238 667 127 1972 1689
Cuts at: 7712 9096 9364 1632
Size: 1384 268 1917
Fragments arranged by size:
1972 1917 1689 1384 728 667 525 268
238 134 127
AfeI AGC'GCT
Cuts at: 5447 6941 5447
Size: 1494 8155
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9452
AflIII A'CryG_T
Cuts at: 1203 3711 8548 9525 1203
Size: 2508 4837 977 1327
Fragments arranged by size:
4837 2508 1327 977
AgeI A'CCGG_T
Cuts at: 4527 8480 4527
Size: 3953 5696
AhdI GACnn_n'nnGTC
Cuts at: 3712 4131 4693 8378 3712
Size: 419 562 3685 4983
Fragments arranged by size:
4983 3685 562 419
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9649
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2291 1184
Size: 1107 8542
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 9617
AlwNI CAG_nnn'CTG
Cuts at: 2155 2569 2599 3376 3379 3966 6567 6718 6740
Size: 414 30 777 3 587 2601 151 22
Cuts at: 6740 7392 8964 2155
Size: 652 1572 2840
Fragments arranged by size:
2840 2601 1572 777 652 587 414 151
30 22 3
ApaI G_GGCC'C
Cuts at: 2392 2722 4811 5121 6388 2392
Size: 330 2089 310 1267 5653
Fragments arranged by size:
5653 2089 1267 330 310
ApaLI G'TGCA_C
Cuts at: 5030 6892 7706 8862 5030
Size: 1862 814 1156 5817
Fragments arranged by size:
5817 1862 1156 814
ApoI r'AATT_y
Cuts at: 1493 6958 7184 7336 9445 1493
Size: 5465 226 152 2109 1697
Fragments arranged by size:
5465 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8203 160
Size: 872 7171 1606
Fragments arranged by size:
7171 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9649
AvaI C'yCGr_G
Cuts at: 1870 1943 2191 2797 3671 4442 4508 4664 4967
Size: 73 248 606 874 771 66 156 303
Cuts at: 4967 5045 5940 6453 6645 6974 1870
Size: 78 895 513 192 329 4545
Fragments arranged by size:
4545 895 874 771 606 513 329 303
248 192 156 78 73 66
BamHI G'GATC_C
Cuts at: 6727 6979 7346 6727
Size: 252 367 9030
Fragments arranged by size:
9030 367 252
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2504 2512 2639 2910 4148
Size: 325 217 627 717 8 127 271 1238
Cuts at: 4148 4820 4972 5071 5710 6179 6349 6970 8425
Size: 672 152 99 639 469 170 621 1455
Cuts at: 8425 618
Size: 1842
Fragments arranged by size:
1842 1455 1238 717 672 639 627 621
469 325 271 217 170 152 127 99
8
BanII G_rGCy'C
Cuts at: 1391 2392 2722 3498 4653 4769 4811 4906 5121
Size: 1001 330 776 1155 116 42 95 215
Cuts at: 5121 5152 5178 5220 5399 6098 6126 6388 6822
Size: 31 26 42 179 699 28 262 434
Cuts at: 6822 6968 1391
Size: 146 4072
Fragments arranged by size:
4072 1155 1001 776 699 434 330 262
215 179 146 116 95 42 42 31
28 26
BbeI G_GCGC'C
Cuts at: 2508 4976 5714 6353 2508
Size: 2468 738 639 5804
Fragments arranged by size:
5804 2468 738 639
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4092 5835 5972 6270 928
Size: 971 2193 1743 137 298 4307
Fragments arranged by size:
4307 2193 1743 971 298 137
BbvCI CC'TCA_GC
Cuts at: 2652 2843 4623 6779 2652
Size: 191 1780 2156 5522
Fragments arranged by size:
5522 2156 1780 191
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2015 2049 6873 6907 7840 7874 1903
Size: 34 78 34 4824 34 933 34 3678
Fragments arranged by size:
4824 3678 933 78 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7541 8751 7541
Size: 1210 8439
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9649
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2240 3750 4937
Size: 107 122 71 631 605 568 1510 1187
Cuts at: 4937 5394 5708 5942 6372 8260 136
Size: 457 314 234 430 1888 1525
Fragments arranged by size:
1888 1525 1510 1187 631 605 568 457
430 314 234 122 107 71
BglII A'GATC_T
Cuts at: 5484 9644 5484
Size: 4160 5489
BlpI GC'TnA_GC
Cuts at: 2135 3152 3818 4231 7066 2135
Size: 1017 666 413 2835 4718
Fragments arranged by size:
4718 2835 1017 666 413
Bme1580I G_kGCm'C
Cuts at: 1790 2392 2478 2517 2722 4508 4811 4823 4968
Size: 602 86 39 205 1786 303 12 145
Cuts at: 4968 5034 5074 5121 6182 6388 6589 6896 7710
Size: 66 40 47 1061 206 201 307 814
Cuts at: 7710 8866 1790
Size: 1156 2573
Fragments arranged by size:
2573 1786 1156 1061 814 602 307 303
206 205 201 145 86 66 47 40
39 12
BmgBI CAC'GTC
Cuts at: 1318 2539 1318
Size: 1221 8428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2656 5282 6140
Size: 416 53 83 399 24 1239 2626 858
Cuts at: 6140 7380 8338 442
Size: 1240 958 1753
Fragments arranged by size:
2626 1753 1240 1239 958 858 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9649
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3373 4651 4654 5434 6511 3373
Size: 1278 3 780 1077 6511
Fragments arranged by size:
6511 1278 1077 780 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2770 3031 3143 3617 3971 4161 4349 4556
Size: 1531 261 112 474 354 190 188 207
Cuts at: 4556 4890 5166 5326 5575 6431 6836 8309 1239
Size: 334 276 160 249 856 405 1473 2579
Fragments arranged by size:
2579 1531 1473 856 474 405 354 334
276 261 249 207 190 188 160 112
Bpu10I CC'TnA_GC
Cuts at: 2652 2843 3575 4623 6779 2652
Size: 191 732 1048 2156 5522
Fragments arranged by size:
5522 2156 1048 732 191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5912 6029 6110 6737 7774 8639 8937 5912
Size: 117 81 627 1037 865 298 6624
Fragments arranged by size:
6624 1037 865 627 298 117 81
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5593 5903 8312 882
Size: 617 4094 310 2409 2219
Fragments arranged by size:
4094 2409 2219 617 310
BsaAI yAC'GTr
Cuts at: 493 1204 6946 7440 493
Size: 711 5742 494 2702
Fragments arranged by size:
5742 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 9643 9643
Size: 9649
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2325 2505 4929 4973
Size: 53 83 186 936 792 180 2424 44
Cuts at: 4973 5711 6350 7456 7838 8473 275
Size: 738 639 1106 382 635 1451
Fragments arranged by size:
2424 1451 1106 936 792 738 639 635
382 186 180 83 53 44
BsaWI w'CCGG_w
Cuts at: 3006 4527 8082 8480 8754 8901 9312 3006
Size: 1521 3555 398 274 147 411 3343
Fragments arranged by size:
3555 3343 1521 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2600 2630 3758 3788 3830
Size: 30 165 30 1197 30 1128 30 42
Cuts at: 3830 3860 5432 5462 1178
Size: 30 1572 30 5365
Fragments arranged by size:
5365 1572 1197 1128 165 42 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2381 2668 2680 2683 4421 4475 4496 4627
Size: 1195 287 12 3 1738 54 21 131
Cuts at: 4627 4640 4643 4742 4751 5543 5816 6376 6519
Size: 13 3 99 9 792 273 560 143
Cuts at: 6519 6542 1186
Size: 23 4293
Fragments arranged by size:
4293 1738 1195 792 560 287 273 143
131 99 54 23 21 13 12 9
3 3
BseYI C'CCAG_C
Cuts at: 2480 2559 2754 3041 3911 4761 4804 5348 5423
Size: 79 195 287 870 850 43 544 75
Cuts at: 5423 6038 6134 6532 6546 6821 8852 9348 2480
Size: 615 96 398 14 275 2031 496 2781
Fragments arranged by size:
2781 2031 870 850 615 544 496 398
287 275 195 96 79 75 43 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2519 4702 5095 5241 5670 6041 6906
Size: 573 783 2183 393 146 429 371 865
Cuts at: 6906 1163
Size: 3906
Fragments arranged by size:
3906 2183 865 783 573 429 393 371
146
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2146 5361 6887 7099 7860 8888
Size: 275 186 145 3215 1526 212 761 1028
Cuts at: 8888 1540
Size: 2301
Fragments arranged by size:
3215 2301 1526 1028 761 275 212 186
145
BsiHKAI G_wGCw'C
Cuts at: 4653 4906 5034 6896 7710 7795 8866 4653
Size: 253 128 1862 814 85 1071 5436
Fragments arranged by size:
5436 1862 1071 814 253 128 85
BsmI GAATG_Cn'
Cuts at: 2656 7160 7253 2656
Size: 4504 93 5052
Fragments arranged by size:
5052 4504 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2392 2478 2517 2722 3498 4508 4653
Size: 399 602 86 39 205 776 1010 145
Cuts at: 4653 4769 4811 4823 4906 4968 5034 5074 5121
Size: 116 42 12 83 62 66 40 47
Cuts at: 5121 5152 5178 5220 5399 6098 6126 6182 6388
Size: 31 26 42 179 699 28 56 206
Cuts at: 6388 6589 6822 6896 6968 7710 7795 8866 1391
Size: 201 233 74 72 742 85 1071 2174
Fragments arranged by size:
2174 1071 1010 776 742 699 602 399
233 206 205 201 179 145 116 86
85 83 74 72 66 62 56 47
42 42 40 39 31 28 26 12
BspEI T'CCGG_A
Cuts at: 3006 9312 3006
Size: 6306 3343
BspHI T'CATG_A
Cuts at: 6258 7538 9268 6258
Size: 1280 1730 6639
Fragments arranged by size:
6639 1730 1280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2434 3306 4478 6703 7004 844
Size: 1590 872 1172 2225 301 3489
Fragments arranged by size:
3489 2225 1590 1172 872 301
BsrBI CCG'CTC
Cuts at: 1394 1823 3066 3078 3151 3480 3579 3763 3796
Size: 429 1243 12 73 329 99 184 33
Cuts at: 3796 7536 1394
Size: 3740 3507
Fragments arranged by size:
3740 3507 1243 429 329 184 99 73
33 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 2610 4060 5788 5881 8144 8318 65
Size: 1183 1362 1450 1728 93 2263 174 1396
Fragments arranged by size:
2263 1728 1450 1396 1362 1183 174 93
BsrFI r'CCGG_y
Cuts at: 1700 1810 2145 2305 2907 4527 4545 4757 5520
Size: 110 335 160 602 1620 18 212 763
Cuts at: 5520 5670 5713 8293 8480 1700
Size: 150 43 2580 187 2869
Fragments arranged by size:
2869 2580 1620 763 602 335 212 187
160 150 110 43 18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9649
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9649
BssSI C'ACGA_G
Cuts at: 3425 7709 8721 3425
Size: 4284 1012 4353
Fragments arranged by size:
4353 4284 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2155 2293 2599 2750 3379 6567 6715 6718
Size: 1361 138 306 151 629 3188 148 3
Cuts at: 6718 794
Size: 3725
Fragments arranged by size:
3725 3188 1361 629 306 151 148 138
3
BstBI TT'CG_AA
Cuts at: 2007 8453 2007
Size: 6446 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9649
BstXI CCAn_nnnn'nTGG
Cuts at: 2708 3241 3629 3784 4327 2708
Size: 533 388 155 543 8030
Fragments arranged by size:
8030 543 533 388 155
BstYI r'GATC_y
Cuts at: 1972 3307 3852 5484 6239 6727 6979 7346 7731
Size: 1335 545 1632 755 488 252 367 385
Cuts at: 7731 7748 9200 9286 9298 9644 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1632 1452 1335 755 545 488 385
367 346 252 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5811 5811
Size: 9649
BtgI C'CryG_G
Cuts at: 513 1065 2053 2235 3069 3628 3806 4095 5000
Size: 552 988 182 834 559 178 289 905
Cuts at: 5000 6118 6388 513
Size: 1118 270 3774
Fragments arranged by size:
3774 1118 988 905 834 559 552 289
270 182 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4963 487
Size: 683 56 3737 5173
Fragments arranged by size:
5173 3737 683 56
BtsI GCAGTG_nn'
Cuts at: 2226 2478 3460 4508 4549 4583 7165 7959 7979
Size: 252 982 1048 41 34 2582 794 20
Cuts at: 7979 2226
Size: 3896
Fragments arranged by size:
3896 2582 1048 982 794 252 41 34
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9649
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5758 5793 6100 6135 6481 6516 529
Size: 35 5194 35 307 35 346 35 3662
Fragments arranged by size:
5194 3662 346 307 35 35 35 35
DraI TTT'AAA
Cuts at: 6953 6953
Size: 9649
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3002 8656 809
Size: 507 1686 5654 1802
Fragments arranged by size:
5654 1802 1686 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 2436 3981
Size: 54 1425 50 275 18 411 195 1545
Cuts at: 3981 4259 4515 5358 6850 7096 7985 9614 8
Size: 278 256 843 1492 246 889 1629 43
Fragments arranged by size:
1629 1545 1492 1425 889 843 411 278
275 256 246 195 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5358 7096 1537
Size: 275 3546 1738 4090
Fragments arranged by size:
4090 3546 1738 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4875 5834 6353 6676 7579 1353
Size: 3522 959 519 323 903 3423
Fragments arranged by size:
3522 3423 959 903 519 323
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5518 6737 6890 8236 8610 8756
Size: 1064 138 3929 1219 153 1346 374 146
Cuts at: 8756 387
Size: 1280
Fragments arranged by size:
3929 1346 1280 1219 1064 374 153 146
138
EcoICRI GAG'CTC
Cuts at: 4651 4904 4651
Size: 253 9396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2360 2770 2885 3019 3031 3143 3257
Size: 393 728 410 115 134 12 112 114
Cuts at: 3257 3617 3924 3971 4051 4161 4349 4556 4890
Size: 360 307 47 80 110 188 207 334
Cuts at: 4890 5166 5326 5575 6023 6431 6836 7712 8309
Size: 276 160 249 448 408 405 876 597
Cuts at: 8309 9096 9364 1239
Size: 787 268 1524
Fragments arranged by size:
1524 876 787 728 597 448 410 408
405 393 360 334 307 276 268 249
207 188 160 134 115 114 112 110
80 47 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3275 3830 3920 4331 5649 3275
Size: 555 90 411 1318 7275
Fragments arranged by size:
7275 1318 555 411 90
EcoO109I rG'GnC_Cy
Cuts at: 2272 2388 2389 2718 3162 3435 3589 3838 4018
Size: 116 1 329 444 273 154 249 180
Cuts at: 4018 4324 4411 4576 4612 4687 4705 4808 4886
Size: 306 87 165 36 75 18 103 78
Cuts at: 4886 5117 5118 5163 5508 5635 5998 6072 6322
Size: 231 1 45 345 127 363 74 250
Cuts at: 6322 6384 6564 2272
Size: 62 180 5357
Fragments arranged by size:
5357 444 363 345 329 306 273 250
249 231 180 180 165 154 127 116
103 87 78 75 74 62 45 36
18 1 1
EcoRI G'AATT_C
Cuts at: 6958 7336 6958
Size: 378 9271
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3958 5719 3958
Size: 1761 7888
FspI TGC'GCA
Cuts at: 8155 8155
Size: 9649
HaeII r_GCGC'y
Cuts at: 1678 2211 2508 3335 3825 3966 4245 4976 5449
Size: 533 297 827 490 141 279 731 473
Cuts at: 5449 5714 6068 6353 6640 6943 8796 1678
Size: 265 354 285 287 303 1853 2531
Fragments arranged by size:
2531 1853 827 731 533 490 473 354
303 297 287 285 279 265 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2600 2632 3665 3697 3698
Size: 32 135 32 1028 32 1033 32 1
Cuts at: 3698 3730 3758 3790 3830 3862 4117 4149 4679
Size: 32 28 32 40 32 255 32 530
Cuts at: 4679 4711 5176 5208 5432 5464 5830 5862 8290
Size: 32 465 32 224 32 366 32 2428
Cuts at: 8290 8322 8364 8396 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2428 1033 1028 530 465 366 255
224 135 42 40 32 32 32 32
32 32 32 32 32 32 32 32
32 32 28 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3649 6993 7239 669
Size: 988 281 1711 3344 246 3079
Fragments arranged by size:
3344 3079 1711 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3894 5011 748
Size: 3146 1117 5386
Fragments arranged by size:
5386 3146 1117
HpaI GTT'AAC
Cuts at: 7239 7239
Size: 9649
KasI G'GCGC_C
Cuts at: 2504 4972 5710 6349 2504
Size: 2468 738 639 5804
Fragments arranged by size:
5804 2468 738 639
KpnI G_GTAC'C
Cuts at: 2643 4152 6974 2643
Size: 1509 2822 5318
Fragments arranged by size:
5318 2822 1509
MfeI C'AATT_G
Cuts at: 7248 7248
Size: 9649
MluI A'CGCG_T
Cuts at: 9525 9525
Size: 9649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3285 3384 5403 6132 6254 8763 8947 1305
Size: 1980 99 2019 729 122 2509 184 2007
Fragments arranged by size:
2509 2019 2007 1980 729 184 122 99
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2438 3983 4261 4517 10
Size: 54 1425 343 606 1545 278 256 5142
Fragments arranged by size:
5142 1545 1425 606 343 278 256 54
MslI CAynn'nnrTG
Cuts at: 518 1166 7607 7966 8125 518
Size: 648 6441 359 159 2042
Fragments arranged by size:
6441 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2152 2237 2773 2806 3109 3376 3511 3808
Size: 546 85 536 33 303 267 135 297
Cuts at: 3808 4039 4223 4235 4693 5626 6409 7065 7742
Size: 231 184 12 458 933 783 656 677
Cuts at: 7742 8890 9135 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 933 783 677 656 546 536
458 303 297 267 245 231 184 135
85 33 12
NaeI GCC'GGC
Cuts at: 2307 2909 4547 4759 5522 2307
Size: 602 1638 212 763 6434
Fragments arranged by size:
6434 1638 763 602 212
NarI GG'CG_CC
Cuts at: 2505 4973 5711 6350 2505
Size: 2468 738 639 5804
Fragments arranged by size:
5804 2468 738 639
NcoI C'CATG_G
Cuts at: 513 1065 2053 6388 513
Size: 552 988 4335 3774
Fragments arranged by size:
4335 3774 988 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9649
NgoMIV G'CCGG_C
Cuts at: 2305 2907 4545 4757 5520 2305
Size: 602 1638 212 763 6434
Fragments arranged by size:
6434 1638 763 602 212
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9649
NotI GC'GGCC_GC
Cuts at: 7096 7096
Size: 9649
NspI r_CATG'y
Cuts at: 1132 1229 3551 3715 4684 6060 7007 8552 1132
Size: 97 2322 164 969 1376 947 1545 2229
Fragments arranged by size:
2322 2229 1545 1376 969 947 164 97
PasI CC'CwG_GG
Cuts at: 1348 2635 4073 4418 5125 1348
Size: 1287 1438 345 707 5872
Fragments arranged by size:
5872 1438 1287 707 345
PciI A'CATG_T
Cuts at: 3711 8548 3711
Size: 4837 4812
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6203 1108
Size: 5095 4554
PfoI T'CCnGG_A
Cuts at: 2328 3431 4209 4401 2328
Size: 1103 778 192 7576
Fragments arranged by size:
7576 1103 778 192
PmeI GTTT'AAAC
Cuts at: 6953 6953
Size: 9649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6653 6685 7718 7750 9231 9263 6653
Size: 32 1033 32 1481 32 7039
Fragments arranged by size:
7039 1481 1033 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3435 3589 3838 4411 4576 4612 4687 5635 3435
Size: 154 249 573 165 36 75 948 7449
Fragments arranged by size:
7449 948 573 249 165 154 75 36
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9649
PsiI TTA'TAA
Cuts at: 2175 7219 2175
Size: 5044 4605
PspOMI G'GGCC_C
Cuts at: 2388 2718 4807 5117 6384 2388
Size: 330 2089 310 1267 5653
Fragments arranged by size:
5653 2089 1267 330 310
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3119 3151 3119
Size: 32 9617
PstI C_TGCA'G
Cuts at: 830 2233 2298 2431 2604 3321 3467 3867 4503
Size: 1403 65 133 173 717 146 400 636
Cuts at: 4503 6330 7001 830
Size: 1827 671 3478
Fragments arranged by size:
3478 1827 1403 717 671 636 400 173
146 133 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9649
PvuII CAG'CTG
Cuts at: 3376 4039 3376
Size: 663 8986
SacI G_AGCT'C
Cuts at: 4653 4906 4653
Size: 253 9396
SacII CC_GC'GG
Cuts at: 2238 3809 2238
Size: 1571 8078
SalI G'TCGA_C
Cuts at: 1936 6991 1936
Size: 5055 4594
SanDI GG'GwC_CC
Cuts at: 5635 5635
Size: 9649
SbfI CC_TGCA'GG
Cuts at: 2298 2431 7001 2298
Size: 133 4570 4946
Fragments arranged by size:
4946 4570 133
ScaI AGT'ACT
Cuts at: 3023 4634 7897 9609 3023
Size: 1611 3263 1712 3063
Fragments arranged by size:
3263 3063 1712 1611
SexAI A'CCwGG_T
Cuts at: 5644 5736 5644
Size: 92 9557
SfcI C'TryA_G
Cuts at: 826 1045 2229 2294 2427 2600 3088 3136 3317
Size: 219 1184 65 133 173 488 48 181
Cuts at: 3317 3463 3863 4188 4499 4812 6326 6613 6997
Size: 146 400 325 311 313 1514 287 384
Cuts at: 6997 8132 8813 9004 826
Size: 1135 681 191 1471
Fragments arranged by size:
1514 1471 1184 1135 681 488 400 384
325 313 311 287 219 191 181 173
146 133 65 48
SfoI GGC'GCC
Cuts at: 2506 4974 5712 6351 2506
Size: 2468 738 639 5804
Fragments arranged by size:
5804 2468 738 639
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9649
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9649
SmaI CCC'GGG
Cuts at: 1872 2799 4444 6976 1872
Size: 927 1645 2532 4545
Fragments arranged by size:
4545 2532 1645 927
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5927 6044 6125 6752 7753 8654
Size: 197 926 3984 117 81 627 1001 901
Cuts at: 8654 8916 820
Size: 262 1553
Fragments arranged by size:
3984 1553 1001 926 901 627 262 197
117 81
SnaBI TAC'GTA
Cuts at: 493 6946 7440 493
Size: 6453 494 2702
Fragments arranged by size:
6453 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9649
SphI G_CATG'C
Cuts at: 1132 3551 4684 7007 1132
Size: 2419 1133 2323 3774
Fragments arranged by size:
3774 2419 2323 1133
SspI AAT'ATT
Cuts at: 5 52 7573 5
Size: 47 7521 2081
Fragments arranged by size:
7521 2081 47
StuI AGG'CCT
Cuts at: 1517 2292 4346 4675 5241 5809 6165 6305 1517
Size: 775 2054 329 566 568 356 140 4861
Fragments arranged by size:
4861 2054 775 568 566 356 329 140
StyI C'CwwG_G
Cuts at: 513 1065 2053 2163 3387 4059 4162 4320 4518
Size: 552 988 110 1224 672 103 158 198
Cuts at: 4518 4701 4722 4752 5331 5406 5868 5970 6388
Size: 183 21 30 579 75 462 102 418
Cuts at: 6388 7088 513
Size: 700 3074
Fragments arranged by size:
3074 1224 988 700 672 579 552 462
418 198 183 158 110 103 102 75
30 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3263 3925 4388 4457 4609 4714 5344
Size: 1255 1683 662 463 69 152 105 630
Cuts at: 5344 5437 5451 7676 7693 7846 8031 8476 325
Size: 93 14 2225 17 153 185 445 1498
Fragments arranged by size:
2225 1683 1498 1255 662 630 463 445
185 153 152 105 93 69 17 14
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3021 4632 5189
Size: 274 80 33 51 1443 1044 1611 557
Cuts at: 5189 6756 7895 9607 96
Size: 1567 1139 1712 138
Fragments arranged by size:
1712 1611 1567 1443 1139 1044 557 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4541 4573 9609 9641 4541
Size: 32 5036 32 4549
Fragments arranged by size:
5036 4549 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9649
XbaI T'CTAG_A
Cuts at: 6985 6985
Size: 9649
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2788 3238 4729 6406 2788
Size: 450 1491 1677 6031
Fragments arranged by size:
6031 1677 1491 450
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9649
XmaI C'CCGG_G
Cuts at: 1870 2797 4442 6974 1870
Size: 927 1645 2532 4545
Fragments arranged by size:
4545 2532 1645 927
XmnI GAAnn'nnTTC
Cuts at: 7778 7778
Size: 9649
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7457 276
Size: 53 83 186 936 5923 2468
Fragments arranged by size:
5923 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII
EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI
RsrII SapI SfiI SrfI SwaI