Restriction map for
pFN21ASDA1730
(Circular) (Six-base) MAPSORT of: pFN21ASDA1730.seq Check: 2565 from: 1 to: 6961
pFN21ASDA1730 6961 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4771 278
Size: 53 83 186 936 3235 2468
Fragments arranged by size:
3235 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3619 4304 1937
Size: 1682 685 4594
Fragments arranged by size:
4594 1682 685
Acc65I G'GTAC_C
Cuts at: 4282 4282
Size: 6961
AclI AA'CG_TT
Cuts at: 1563 5088 5461 1563
Size: 3525 373 3063
Fragments arranged by size:
3525 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2479 2583 3035 3750 5024 6408 6676 1632
Size: 847 104 452 715 1274 1384 268 1917
Fragments arranged by size:
1917 1384 1274 847 715 452 268 104
AfeI AGC'GCT
Cuts at: 2489 2489
Size: 6961
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6764
AflIII A'CryG_T
Cuts at: 1203 4048 5860 6837 1203
Size: 2845 1812 977 1327
Fragments arranged by size:
2845 1812 1327 977
AgeI A'CCGG_T
Cuts at: 5792 5792
Size: 6961
AhdI GACnn_n'nnGTC
Cuts at: 3879 5690 3879
Size: 1811 5150
AleI CACnn'nnGTG
Cuts at: 1166 2363 1166
Size: 1197 5764
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2279 3183 3222 1184
Size: 1095 904 39 4923
Fragments arranged by size:
4923 1095 904 39
AlwNI CAG_nnn'CTG
Cuts at: 2443 2710 4704 6276 2443
Size: 267 1994 1572 3128
Fragments arranged by size:
3128 1994 1572 267
ApaLI G'TGCA_C
Cuts at: 3579 5018 6174 3579
Size: 1439 1156 4366
Fragments arranged by size:
4366 1439 1156
ApoI r'AATT_y
Cuts at: 1493 2092 2452 2945 3105 3148 3544 3566 3895
Size: 599 360 493 160 43 396 22 329
Cuts at: 3895 4270 4496 4648 6757 1493
Size: 375 226 152 2109 1697
Fragments arranged by size:
2109 1697 599 493 396 375 360 329
226 160 152 43 22
AseI AT'TA_AT
Cuts at: 160 1032 3755 5515 160
Size: 872 2723 1760 1606
Fragments arranged by size:
2723 1760 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6961
AvaI C'yCGr_G
Cuts at: 1870 1943 2027 2471 4286 1870
Size: 73 84 444 1815 4545
Fragments arranged by size:
4545 1815 444 84 73
BamHI G'GATC_C
Cuts at: 2421 2476 3330 4291 4658 2421
Size: 55 854 961 367 4724
Fragments arranged by size:
4724 961 854 367 55
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4282 5737 618
Size: 325 217 627 2495 1455 1842
Fragments arranged by size:
2495 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3495 4280 1391
Size: 2104 785 4072
Fragments arranged by size:
4072 2104 785
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2559 928
Size: 971 660 5330
Fragments arranged by size:
5330 971 660
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5152 5186 1903
Size: 34 3215 34 3678
Fragments arranged by size:
3678 3215 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4853 6063 4853
Size: 1210 5751
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6961
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5572 136
Size: 107 122 71 631 605 3900 1525
Fragments arranged by size:
3900 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2298 6956 2298
Size: 4658 2303
BlpI GC'TnA_GC
Cuts at: 4378 4378
Size: 6961
Bme1580I G_kGCm'C
Cuts at: 1790 3583 5022 6178 1790
Size: 1793 1439 1156 2573
Fragments arranged by size:
2573 1793 1439 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6961
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3135 4692 5650
Size: 416 53 83 399 24 1718 1557 958
Cuts at: 5650 442
Size: 1753
Fragments arranged by size:
1753 1718 1557 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6961
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3915 4236 3915
Size: 321 6640
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2184 2420 3927 5621 1239
Size: 945 236 1507 1694 2579
Fragments arranged by size:
2579 1694 1507 945 236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2132 2639 5086 5951 6249 2132
Size: 507 2447 865 298 2844
Fragments arranged by size:
2844 2447 865 507 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5624 882
Size: 617 4125 2219
Fragments arranged by size:
4125 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2857 4258 4752 493
Size: 711 1653 1401 494 2702
Fragments arranged by size:
2702 1653 1401 711 494
BsaBI GATnn'nnATC
Cuts at: 6955 6955
Size: 6961
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2060 4768 5150 5785
Size: 53 83 186 936 527 2708 382 635
Cuts at: 5785 275
Size: 1451
Fragments arranged by size:
2708 1451 936 635 527 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2316 3327 5394 5792 6066 6213 6624 2316
Size: 1011 2067 398 274 147 411 2653
Fragments arranged by size:
2653 2067 1011 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2045 2075 1178
Size: 30 165 30 642 30 6064
Fragments arranged by size:
6064 642 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 1186
Size: 883 6078
BseYI C'CCAG_C
Cuts at: 6164 6660 6164
Size: 496 6465
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3459 1163
Size: 573 1723 4665
Fragments arranged by size:
4665 1723 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4411 5172 6200 1540
Size: 275 186 2410 761 1028 2301
Fragments arranged by size:
2410 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3495 3583 5022 5107 6178 3495
Size: 88 1439 85 1071 4278
Fragments arranged by size:
4278 1439 1071 88 85
BsmI GAATG_Cn'
Cuts at: 2283 2374 3035 3916 4472 4565 2283
Size: 91 661 881 556 93 4679
Fragments arranged by size:
4679 881 661 556 93 91
BsmBI CGTCTCn'nnnn_
Cuts at: 2067 2067
Size: 6961
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3495 3583 4280 5022 5107 6178 1391
Size: 399 1705 88 697 742 85 1071 2174
Fragments arranged by size:
2174 1705 1071 742 697 399 88 85
BspEI T'CCGG_A
Cuts at: 2316 6624 2316
Size: 4308 2653
BspHI T'CATG_A
Cuts at: 3654 4132 4850 6580 3654
Size: 478 718 1730 4035
Fragments arranged by size:
4035 1730 718 478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4316 844
Size: 3472 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4848 1394
Size: 429 3025 3507
Fragments arranged by size:
3507 3025 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2573 5456 5630 65
Size: 1183 1325 2883 174 1396
Fragments arranged by size:
2883 1396 1325 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2039 2499 5605 5792 1700
Size: 110 229 460 3106 187 2869
Fragments arranged by size:
3106 2869 460 229 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6961
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6961
BssSI C'ACGA_G
Cuts at: 5021 6033 5021
Size: 1012 5949
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6961
BstBI TT'CG_AA
Cuts at: 2007 5765 2007
Size: 3758 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6961
BstYI r'GATC_y
Cuts at: 1972 2298 2421 2476 3202 3330 4291 4658 5043
Size: 326 123 55 726 128 961 367 385
Cuts at: 5043 5060 6512 6598 6610 6956 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 961 726 385 367 346 326
128 123 86 55 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2049 2205 3264 4207 513
Size: 552 984 156 1059 943 3267
Fragments arranged by size:
3267 1059 984 943 552 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6222
Fragments arranged by size:
6222 683 56
BtsI GCAGTG_nn'
Cuts at: 2625 3328 3389 4477 5271 5291 2625
Size: 703 61 1088 794 20 4295
Fragments arranged by size:
4295 1088 794 703 61 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6961
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6926
DraI TTT'AAA
Cuts at: 2688 3111 4265 2688
Size: 423 1154 5384
Fragments arranged by size:
5384 1154 423
DraIII CAC_nnn'GTG
Cuts at: 3802 4201 3802
Size: 399 6562
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5968 809
Size: 507 4652 1802
Fragments arranged by size:
4652 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3935 4170 4408
Size: 54 1425 50 275 18 2105 235 238
Cuts at: 4408 5297 6926 8
Size: 889 1629 43
Fragments arranged by size:
2105 1629 1425 889 275 238 235 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4408 1537
Size: 275 2596 4090
Fragments arranged by size:
4090 2596 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2793 3500 3656 4891 1353
Size: 1440 707 156 1235 3423
Fragments arranged by size:
3423 1440 1235 707 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5548 5922 6068 387
Size: 1064 138 3959 374 146 1280
Fragments arranged by size:
3959 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3493 3493
Size: 6961
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2184 2420 2479 2583 3035 3750 3927
Size: 393 552 236 59 104 452 715 177
Cuts at: 3927 5024 5621 6408 6676 1239
Size: 1097 597 787 268 1524
Fragments arranged by size:
1524 1097 787 715 597 552 452 393
268 236 177 104 59
EcoO109I rG'GnC_Cy
Cuts at: 3969 3969
Size: 6961
EcoRI G'AATT_C
Cuts at: 2452 2945 3895 4270 4648 2452
Size: 493 950 375 378 4765
Fragments arranged by size:
4765 950 493 378 375
EcoRV GAT'ATC
Cuts at: 2083 2377 2752 2083
Size: 294 375 6292
Fragments arranged by size:
6292 375 294
FspI TGC'GCA
Cuts at: 5467 5467
Size: 6961
HaeII r_GCGC'y
Cuts at: 1678 2491 3177 6108 1678
Size: 813 686 2931 2531
Fragments arranged by size:
2931 2531 813 686
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2045 2077 3865 3897 5602
Size: 32 135 32 473 32 1788 32 1705
Cuts at: 5602 5634 5676 5708 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1788 1705 473 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4305 4551 669
Size: 988 281 2367 246 3079
Fragments arranged by size:
3079 2367 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2322 3113 3371 3455 3867 748
Size: 1574 791 258 84 412 3842
Fragments arranged by size:
3842 1574 791 412 258 84
HpaI GTT'AAC
Cuts at: 4551 4551
Size: 6961
KpnI G_GTAC'C
Cuts at: 4286 4286
Size: 6961
MfeI C'AATT_G
Cuts at: 4560 4560
Size: 6961
MluI A'CGCG_T
Cuts at: 6837 6837
Size: 6961
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3250 3632 4180 6075 6259 1305
Size: 1945 382 548 1895 184 2007
Fragments arranged by size:
2007 1945 1895 548 382 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3937 4172 10
Size: 54 1425 343 2105 235 2799
Fragments arranged by size:
2799 2105 1425 343 235 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 4919 5278 5437 518
Size: 648 1197 2556 359 159 2042
Fragments arranged by size:
2556 2042 1197 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3830 4377 5054 6202 6447 1606
Size: 2224 547 677 1148 245 2120
Fragments arranged by size:
2224 2120 1148 677 547 245
NaeI GCC'GGC
Cuts at: 2041 2501 2041
Size: 460 6501
NcoI C'CATG_G
Cuts at: 513 1065 2049 2205 3264 4207 513
Size: 552 984 156 1059 943 3267
Fragments arranged by size:
3267 1059 984 943 552 156
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6961
NgoMIV G'CCGG_C
Cuts at: 2039 2499 2039
Size: 460 6501
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6961
NotI GC'GGCC_GC
Cuts at: 4408 4408
Size: 6961
NspI r_CATG'y
Cuts at: 1132 1229 2905 3415 4052 4087 4319 5864 1132
Size: 97 1676 510 637 35 232 1545 2229
Fragments arranged by size:
2229 1676 1545 637 510 232 97 35
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6961
PciI A'CATG_T
Cuts at: 4048 5860 4048
Size: 1812 5149
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2443 2710 3995 1108
Size: 1335 267 1285 4074
Fragments arranged by size:
4074 1335 1285 267
PmeI GTTT'AAAC
Cuts at: 4265 4265
Size: 6961
PmlI CAC'GTG
Cuts at: 2857 2857
Size: 6961
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5030 5062 6543 6575 5030
Size: 32 1481 32 5416
Fragments arranged by size:
5416 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3969 3969
Size: 6961
PshAI GACnn'nnGTC
Cuts at: 1613 2656 1613
Size: 1043 5918
PsiI TTA'TAA
Cuts at: 4531 4531
Size: 6961
PspXI vC'TCGA_Gb
Cuts at: 2471 2471
Size: 6961
PstI C_TGCA'G
Cuts at: 830 2157 3214 4058 4313 830
Size: 1327 1057 844 255 3478
Fragments arranged by size:
3478 1327 1057 844 255
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6961
PvuII CAG'CTG
Cuts at: 3830 3830
Size: 6961
SacI G_AGCT'C
Cuts at: 3495 3495
Size: 6961
SalI G'TCGA_C
Cuts at: 1936 4303 1936
Size: 2367 4594
SapI GCTCTTCn'nnn_
Cuts at: 2793 3500 2793
Size: 707 6254
SbfI CC_TGCA'GG
Cuts at: 4313 4313
Size: 6961
ScaI AGT'ACT
Cuts at: 5209 6921 5209
Size: 1712 5249
SfcI C'TryA_G
Cuts at: 826 1045 2153 3210 3462 4054 4309 5444 6125
Size: 219 1108 1057 252 592 255 1135 681
Cuts at: 6125 6316 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1108 1057 681 592 255 252
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6961
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6961
SmaI CCC'GGG
Cuts at: 1872 4288 1872
Size: 2416 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2111 2471 2654 5065 5966 6228
Size: 197 926 168 360 183 2411 901 262
Cuts at: 6228 820
Size: 1553
Fragments arranged by size:
2411 1553 926 901 360 262 197 183
168
SnaBI TAC'GTA
Cuts at: 493 4258 4752 493
Size: 3765 494 2702
Fragments arranged by size:
3765 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6961
SphI G_CATG'C
Cuts at: 1132 2905 4087 4319 1132
Size: 1773 1182 232 3774
Fragments arranged by size:
3774 1773 1182 232
SspI AAT'ATT
Cuts at: 5 52 4885 5
Size: 47 4833 2081
Fragments arranged by size:
4833 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6961
StyI C'CwwG_G
Cuts at: 513 1065 2049 2205 3264 3637 4207 4400 513
Size: 552 984 156 1059 373 570 193 3074
Fragments arranged by size:
3074 1059 984 570 552 373 193 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2664 4988 5005 5158 5343 5788 325
Size: 1255 1084 2324 17 153 185 445 1498
Fragments arranged by size:
2324 1498 1255 1084 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3733 5207 6919
Size: 274 80 33 51 1443 1756 1474 1712
Cuts at: 6919 96
Size: 138
Fragments arranged by size:
1756 1712 1474 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6921 6953 6921
Size: 32 6929
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6961
XbaI T'CTAG_A
Cuts at: 3664 4297 3664
Size: 633 6328
XhoI C'TCGA_G
Cuts at: 1943 2471 1943
Size: 528 6433
XmaI C'CCGG_G
Cuts at: 1870 4286 1870
Size: 2416 4545
XmnI GAAnn'nnTTC
Cuts at: 2324 3273 5090 2324
Size: 949 1817 4195
Fragments arranged by size:
4195 1817 949
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4769 276
Size: 53 83 186 936 3235 2468
Fragments arranged by size:
3235 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI