(Circular) (Six-base) MAPSORT of: pFN21ASDA1623.seq Check: 1280 from: 1 to: 8425 pFN21ASDA1623 8425 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2632 3642 2632 Size: 1010 7415 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6235 278 Size: 53 83 186 936 4699 2468 Fragments arranged by size: 4699 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2802 2906 5768 1937 Size: 865 104 2862 4594 Fragments arranged by size: 4594 2862 865 104 Acc65I G'GTAC_C Cuts at: 5746 5746 Size: 8425 AclI AA'CG_TT Cuts at: 1563 6552 6925 1563 Size: 4989 373 3063 Fragments arranged by size: 4989 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3884 6488 7872 8140 1632 Size: 2252 2604 1384 268 1917 Fragments arranged by size: 2604 2252 1917 1384 268 AfeI AGC'GCT Cuts at: 3416 5361 3416 Size: 1945 6480 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8228 AflIII A'CryG_T Cuts at: 1203 2333 2787 4251 7324 8301 1203 Size: 1130 454 1464 3073 977 1327 Fragments arranged by size: 3073 1464 1327 1130 977 454 AgeI A'CCGG_T Cuts at: 4161 7256 4161 Size: 3095 5330 AhdI GACnn_n'nnGTC Cuts at: 7154 7154 Size: 8425 AleI CACnn'nnGTG Cuts at: 1166 2334 3953 1166 Size: 1168 1619 5638 Fragments arranged by size: 5638 1619 1168 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4409 1184 Size: 3225 5200 AlwNI CAG_nnn'CTG Cuts at: 2846 3172 5524 5584 5712 6168 7740 2846 Size: 326 2352 60 128 456 1572 3531 Fragments arranged by size: 3531 2352 1572 456 326 128 60 ApaI G_GGCC'C Cuts at: 4095 4106 5484 5565 4095 Size: 11 1378 81 6955 Fragments arranged by size: 6955 1378 81 11 ApaLI G'TGCA_C Cuts at: 4384 6482 7638 4384 Size: 2098 1156 5171 Fragments arranged by size: 5171 2098 1156 ApoI r'AATT_y Cuts at: 1493 2545 4982 5734 5960 6112 8221 1493 Size: 1052 2437 752 226 152 2109 1697 Fragments arranged by size: 2437 2109 1697 1052 752 226 152 AseI AT'TA_AT Cuts at: 160 1032 6979 160 Size: 872 5947 1606 Fragments arranged by size: 5947 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8425 AvaI C'yCGr_G Cuts at: 1870 1943 2297 3549 4568 4624 5120 5202 5648 Size: 73 354 1252 1019 56 496 82 446 Cuts at: 5648 5750 1870 Size: 102 4545 Fragments arranged by size: 4545 1252 1019 496 446 354 102 82 73 56 BamHI G'GATC_C Cuts at: 5641 5755 6122 5641 Size: 114 367 7944 Fragments arranged by size: 7944 367 114 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2200 2435 3501 3579 Size: 325 217 627 255 158 235 1066 78 Cuts at: 3579 3654 3676 4933 5198 5375 5504 5746 7201 Size: 75 22 1257 265 177 129 242 1455 Cuts at: 7201 618 Size: 1842 Fragments arranged by size: 1842 1455 1257 1066 627 325 265 255 242 235 217 177 158 129 78 75 22 BanII G_rGCy'C Cuts at: 1391 2304 3556 3858 3947 4021 4044 4095 4106 Size: 913 1252 302 89 74 23 51 11 Cuts at: 4106 4538 4826 5411 5484 5565 5744 1391 Size: 432 288 585 73 81 179 4072 Fragments arranged by size: 4072 1252 913 585 432 302 288 179 89 81 74 73 51 23 11 BbeI G_GCGC'C Cuts at: 2046 3505 3583 3680 5379 2046 Size: 1459 78 97 1699 5092 Fragments arranged by size: 5092 1699 1459 97 78 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2201 2806 3127 928 Size: 971 302 605 321 6226 Fragments arranged by size: 6226 971 605 321 302 BbvCI CC'TCA_GC Cuts at: 3659 5096 5313 3659 Size: 1437 217 6771 Fragments arranged by size: 6771 1437 217 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2144 2178 6616 6650 1903 Size: 34 207 34 4438 34 3678 Fragments arranged by size: 4438 3678 207 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6317 7527 6317 Size: 1210 7215 BclI T'GATC_A Cuts at: 1548 2848 3444 1548 Size: 1300 596 6529 Fragments arranged by size: 6529 1300 596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4906 7036 136 Size: 107 122 71 631 605 3234 2130 1525 Fragments arranged by size: 3234 2130 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8420 8420 Size: 8425 BlpI GC'TnA_GC Cuts at: 2943 3179 3882 4532 5208 5842 2943 Size: 236 703 650 676 634 5526 Fragments arranged by size: 5526 703 676 650 634 236 Bme1580I G_kGCm'C Cuts at: 1790 2054 2438 4095 4106 4388 5484 5543 5565 Size: 264 384 1657 11 282 1096 59 22 Cuts at: 5565 6486 7642 1790 Size: 921 1156 2573 Fragments arranged by size: 2573 1657 1156 1096 921 384 282 264 59 22 11 BmgBI CAC'GTC Cuts at: 1318 2055 2221 2790 1318 Size: 737 166 569 6953 Fragments arranged by size: 6953 737 569 166 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3001 5050 6156 Size: 416 53 83 399 24 1584 2049 1106 Cuts at: 6156 7114 442 Size: 958 1753 Fragments arranged by size: 2049 1753 1584 1106 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3275 1056 Size: 2219 6206 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2053 2588 4213 4876 5365 2053 Size: 535 1625 663 489 5113 Fragments arranged by size: 5113 1625 663 535 489 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2612 3329 3446 3738 3953 3989 4244 Size: 842 531 717 117 292 215 36 255 Cuts at: 4244 4676 7085 1239 Size: 432 2409 2579 Fragments arranged by size: 2579 2409 842 717 531 432 292 255 215 117 36 Bpu10I CC'TnA_GC Cuts at: 3374 3569 3659 5096 5313 5350 3374 Size: 195 90 1437 217 37 6449 Fragments arranged by size: 6449 1437 217 195 90 37 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3386 4574 5096 6550 7415 7713 3386 Size: 1188 522 1454 865 298 4098 Fragments arranged by size: 4098 1454 1188 865 522 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4077 5117 5645 7088 882 Size: 617 2578 1040 528 1443 2219 Fragments arranged by size: 2578 2219 1443 1040 617 528 BsaAI yAC'GTr Cuts at: 493 1204 2364 2627 4606 5722 6216 493 Size: 711 1160 263 1979 1116 494 2702 Fragments arranged by size: 2702 1979 1160 1116 711 494 263 BsaBI GATnn'nnATC Cuts at: 8419 8419 Size: 8425 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2043 2114 3502 3580 Size: 53 83 186 936 510 71 1388 78 Cuts at: 3580 3677 3901 4371 5376 5595 6232 6614 7249 Size: 97 224 470 1005 219 637 382 635 Cuts at: 7249 275 Size: 1451 Fragments arranged by size: 1451 1388 1005 936 637 635 510 470 382 224 219 186 97 83 78 71 53 BsaWI w'CCGG_w Cuts at: 2117 4161 6858 7256 7530 7677 8088 2117 Size: 2044 2697 398 274 147 411 2454 Fragments arranged by size: 2697 2454 2044 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5029 5059 1178 Size: 30 165 30 3626 30 4544 Fragments arranged by size: 4544 3626 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2471 3008 3065 3698 3701 3704 3707 3752 Size: 1285 537 57 633 3 3 3 45 Cuts at: 3752 3782 3800 3803 3806 3815 3824 3833 3836 Size: 30 18 3 3 9 9 9 3 Cuts at: 3836 4010 4202 4577 4754 4757 4760 4820 4837 Size: 174 192 375 177 3 3 60 17 Cuts at: 4837 5299 5330 1186 Size: 462 31 4281 Fragments arranged by size: 4281 1285 633 537 462 375 192 177 174 60 57 45 31 30 18 17 9 9 9 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2395 2776 2806 3520 4203 4629 4701 5258 5577 Size: 381 30 714 683 426 72 557 319 Cuts at: 5577 5656 5675 5681 5705 7628 8124 2395 Size: 79 19 6 24 1923 496 2696 Fragments arranged by size: 2696 1923 714 683 557 496 426 381 319 79 72 30 24 19 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2717 4301 1163 Size: 573 981 1584 5287 Fragments arranged by size: 5287 1584 981 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2247 4166 5875 6636 7664 1540 Size: 275 186 246 1919 1709 761 1028 2301 Fragments arranged by size: 2301 1919 1709 1028 761 275 246 186 BsiHKAI G_wGCw'C Cuts at: 2304 4302 4388 4569 4575 4770 6486 6571 7642 Size: 1998 86 181 6 195 1716 85 1071 Cuts at: 7642 2304 Size: 3087 Fragments arranged by size: 3087 1998 1716 1071 195 181 86 85 6 BsmI GAATG_Cn' Cuts at: 2292 5936 6029 2292 Size: 3644 93 4688 Fragments arranged by size: 4688 3644 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3206 4144 5586 3206 Size: 938 1442 6045 Fragments arranged by size: 6045 1442 938 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2304 2438 3556 3858 3947 4021 Size: 399 264 250 134 1118 302 89 74 Cuts at: 4021 4044 4095 4106 4302 4388 4538 4569 4575 Size: 23 51 11 196 86 150 31 6 Cuts at: 4575 4770 4826 5411 5484 5543 5565 5744 6486 Size: 195 56 585 73 59 22 179 742 Cuts at: 6486 6571 7642 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1118 1071 742 585 399 302 264 250 196 195 179 150 134 89 86 85 74 73 59 56 51 31 23 22 11 6 BspEI T'CCGG_A Cuts at: 2117 8088 2117 Size: 5971 2454 BspHI T'CATG_A Cuts at: 2682 4947 6314 8044 2682 Size: 2265 1367 1730 3063 Fragments arranged by size: 3063 2265 1730 1367 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2632 3642 5780 844 Size: 1788 1010 2138 3489 Fragments arranged by size: 3489 2138 1788 1010 BsrBI CCG'CTC Cuts at: 1394 1823 3712 6312 1394 Size: 429 1889 2600 3507 Fragments arranged by size: 3507 2600 1889 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6920 7094 65 Size: 1183 5672 174 1396 Fragments arranged by size: 5672 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3303 3498 4161 5293 5459 7069 7256 Size: 110 1493 195 663 1132 166 1610 187 Cuts at: 7256 1700 Size: 2869 Fragments arranged by size: 2869 1610 1493 1132 663 195 187 166 110 BsrGI T'GTAC_A Cuts at: 96 2829 96 Size: 2733 5692 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8425 BssSI C'ACGA_G Cuts at: 2657 4688 6485 7497 2657 Size: 2031 1797 1012 3585 Fragments arranged by size: 3585 2031 1797 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2548 794 Size: 1754 6671 BstBI TT'CG_AA Cuts at: 2007 7229 2007 Size: 5222 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8425 BstXI CCAn_nnnn'nTGG Cuts at: 3955 3955 Size: 8425 BstYI r'GATC_y Cuts at: 1972 4359 5641 5755 6122 6507 6524 7976 8062 Size: 2387 1282 114 367 385 17 1452 86 Cuts at: 8062 8074 8420 1972 Size: 12 346 1977 Fragments arranged by size: 2387 1977 1452 1282 385 367 346 114 86 17 12 BstZ17I GTA'TAC Cuts at: 2803 2803 Size: 8425 Bsu36I CC'TnA_GG Cuts at: 3219 3219 Size: 8425 BtgI C'CryG_G Cuts at: 513 1065 2022 2440 2988 3511 4455 4894 5370 Size: 552 957 418 548 523 944 439 476 Cuts at: 5370 5553 513 Size: 183 3385 Fragments arranged by size: 3385 957 944 552 548 523 476 439 418 183 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4691 487 Size: 683 56 3465 4221 Fragments arranged by size: 4221 3465 683 56 BtsI GCAGTG_nn' Cuts at: 2418 4881 4970 5269 5477 5941 6735 6755 2418 Size: 2463 89 299 208 464 794 20 4088 Fragments arranged by size: 4088 2463 794 464 299 208 89 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8425 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8390 DraI TTT'AAA Cuts at: 5729 5729 Size: 8425 DraIII CAC_nnn'GTG Cuts at: 2581 5549 2581 Size: 2968 5457 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7432 809 Size: 507 6116 1802 Fragments arranged by size: 6116 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 3432 3990 Size: 54 1425 50 275 18 195 1407 558 Cuts at: 3990 4047 4408 4429 4495 4608 4635 4647 4798 Size: 57 361 21 66 113 27 12 151 Cuts at: 4798 4891 5243 5526 5556 5872 6761 8390 8 Size: 93 352 283 30 316 889 1629 43 Fragments arranged by size: 1629 1425 1407 889 558 361 352 316 283 275 195 151 113 93 66 57 54 50 43 30 27 21 18 12 EagI C'GGCC_G Cuts at: 1537 1812 5872 1537 Size: 275 4060 4090 Fragments arranged by size: 4090 4060 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2894 3089 3386 3801 4307 4526 5189 5334 Size: 1541 195 297 415 506 219 663 145 Cuts at: 5334 6355 1353 Size: 1021 3423 Fragments arranged by size: 3423 1541 1021 663 506 415 297 219 195 145 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2306 3626 4025 4049 4300 7012 Size: 1064 138 717 1320 399 24 251 2712 Cuts at: 7012 7386 7532 387 Size: 374 146 1280 Fragments arranged by size: 2712 1320 1280 1064 717 399 374 251 146 138 24 EcoICRI GAG'CTC Cuts at: 2302 2302 Size: 8425 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2612 3329 3446 3738 3884 3953 Size: 393 449 531 717 117 292 146 69 Cuts at: 3953 3989 4244 4676 6488 7085 7872 8140 1239 Size: 36 255 432 1812 597 787 268 1524 Fragments arranged by size: 1812 1524 787 717 597 531 449 432 393 292 268 255 146 117 69 36 EcoNI CCTnn'n_nnAGG Cuts at: 3218 3932 3992 5312 3218 Size: 714 60 1320 6331 Fragments arranged by size: 6331 1320 714 60 EcoO109I rG'GnC_Cy Cuts at: 2082 3478 3838 3925 4091 4102 4923 5253 5305 Size: 1396 360 87 166 11 821 330 52 Cuts at: 5305 5481 5521 5562 2082 Size: 176 40 41 4945 Fragments arranged by size: 4945 1396 821 360 330 176 166 87 52 41 40 11 EcoRI G'AATT_C Cuts at: 5734 6112 5734 Size: 378 8047 EcoRV GAT'ATC Cuts at: 4621 4621 Size: 8425 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3892 3892 Size: 8425 FspI TGC'GCA Cuts at: 2405 6931 2405 Size: 4526 3899 HaeII r_GCGC'y Cuts at: 1678 2046 3318 3418 3505 3583 3680 3704 4331 Size: 368 1272 100 87 78 97 24 627 Cuts at: 4331 4519 4669 4696 4759 4918 5363 5379 5663 Size: 188 150 27 63 159 445 16 284 Cuts at: 5663 7572 1678 Size: 1909 2531 Fragments arranged by size: 2531 1909 1272 627 445 368 284 188 159 150 100 97 87 78 63 27 24 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5201 5233 7066 7098 7140 Size: 32 135 32 3629 32 1833 32 42 Cuts at: 7140 7172 1373 Size: 32 2626 Fragments arranged by size: 3629 2626 1833 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2907 3364 4345 4480 5769 6015 Size: 988 281 969 457 981 135 1289 246 Cuts at: 6015 669 Size: 3079 Fragments arranged by size: 3079 1289 988 981 969 457 281 246 135 HindIII A'AGCT_T Cuts at: 748 2132 748 Size: 1384 7041 HpaI GTT'AAC Cuts at: 6015 6015 Size: 8425 KasI G'GCGC_C Cuts at: 2042 3501 3579 3676 5375 2042 Size: 1459 78 97 1699 5092 Fragments arranged by size: 5092 1699 1459 97 78 KpnI G_GTAC'C Cuts at: 5750 5750 Size: 8425 MfeI C'AATT_G Cuts at: 6024 6024 Size: 8425 MluI A'CGCG_T Cuts at: 2333 8301 2333 Size: 5968 2457 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4149 5244 7539 7723 1305 Size: 2844 1095 2295 184 2007 Fragments arranged by size: 2844 2295 2007 1095 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3434 4610 4649 4893 10 Size: 54 1425 343 1602 1176 39 244 3542 Fragments arranged by size: 3542 1602 1425 1176 343 244 54 39 MslI CAynn'nnrTG Cuts at: 518 1166 2285 2334 2956 3953 4604 6383 6742 Size: 648 1119 49 622 997 651 1779 359 Cuts at: 6742 6901 518 Size: 159 2042 Fragments arranged by size: 2042 1779 1119 997 651 648 622 359 159 49 MspA1I CmG'CkG Cuts at: 1606 2442 2810 3091 3163 3513 3636 3663 4399 Size: 836 368 281 72 350 123 27 736 Cuts at: 4399 4510 5434 5531 5709 5841 6518 7666 7911 Size: 111 924 97 178 132 677 1148 245 Cuts at: 7911 1606 Size: 2120 Fragments arranged by size: 2120 1148 924 836 736 677 368 350 281 245 178 132 123 111 97 72 27 NaeI GCC'GGC Cuts at: 5461 5461 Size: 8425 NarI GG'CG_CC Cuts at: 2043 3502 3580 3677 5376 2043 Size: 1459 78 97 1699 5092 Fragments arranged by size: 5092 1699 1459 97 78 NcoI C'CATG_G Cuts at: 513 1065 2022 4455 5553 513 Size: 552 957 2433 1098 3385 Fragments arranged by size: 3385 2433 1098 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8425 NgoMIV G'CCGG_C Cuts at: 5459 5459 Size: 8425 NheI G'CTAG_C Cuts at: 1052 3271 1052 Size: 2219 6206 NotI GC'GGCC_GC Cuts at: 5872 5872 Size: 8425 NspI r_CATG'y Cuts at: 1132 1229 5783 7328 1132 Size: 97 4554 1545 2229 Fragments arranged by size: 4554 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4919 5484 1348 Size: 3571 565 4289 Fragments arranged by size: 4289 3571 565 PciI A'CATG_T Cuts at: 7324 7324 Size: 8425 PflMI CCAn_nnn'nTGG Cuts at: 1108 4417 5524 5712 1108 Size: 3309 1107 188 3821 Fragments arranged by size: 3821 3309 1107 188 PfoI T'CCnGG_A Cuts at: 2589 2610 3843 3930 4257 2589 Size: 21 1233 87 327 6757 Fragments arranged by size: 6757 1233 327 87 21 PmeI GTTT'AAAC Cuts at: 5729 5729 Size: 8425 PmlI CAC'GTG Cuts at: 2627 4606 2627 Size: 1979 6446 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4238 4270 6494 6526 8007 8039 4238 Size: 32 2224 32 1481 32 4624 Fragments arranged by size: 4624 2224 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3478 4923 5253 3478 Size: 1445 330 6650 Fragments arranged by size: 6650 1445 330 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8425 PsiI TTA'TAA Cuts at: 5995 5995 Size: 8425 PspOMI G'GGCC_C Cuts at: 4091 4102 5480 5561 4091 Size: 11 1378 81 6955 Fragments arranged by size: 6955 1378 81 11 PspXI vC'TCGA_Gb Cuts at: 4568 4568 Size: 8425 PstI C_TGCA'G Cuts at: 830 2107 2157 2544 3168 3486 4398 4515 4977 Size: 1277 50 387 624 318 912 117 462 Cuts at: 4977 5023 5777 830 Size: 46 754 3478 Fragments arranged by size: 3478 1277 912 754 624 462 387 318 117 50 46 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8425 PvuII CAG'CTG Cuts at: 2810 3091 3163 4510 2810 Size: 281 72 1347 6725 Fragments arranged by size: 6725 1347 281 72 SacI G_AGCT'C Cuts at: 2304 2304 Size: 8425 SacII CC_GC'GG Cuts at: 2443 3514 2443 Size: 1071 7354 SalI G'TCGA_C Cuts at: 1936 2905 5767 1936 Size: 969 2862 4594 Fragments arranged by size: 4594 2862 969 SanDI GG'GwC_CC Cuts at: 4923 5253 4923 Size: 330 8095 SapI GCTCTTCn'nnn_ Cuts at: 3089 4307 5189 3089 Size: 1218 882 6325 Fragments arranged by size: 6325 1218 882 SbfI CC_TGCA'GG Cuts at: 2157 5777 2157 Size: 3620 4805 ScaI AGT'ACT Cuts at: 6673 8385 6673 Size: 1712 6713 SexAI A'CCwGG_T Cuts at: 2169 2169 Size: 8425 SfcI C'TryA_G Cuts at: 826 1045 2103 2153 2205 2540 3164 3482 4394 Size: 219 1058 50 52 335 624 318 912 Cuts at: 4394 4511 4973 5019 5773 6908 7589 7780 826 Size: 117 462 46 754 1135 681 191 1471 Fragments arranged by size: 1471 1135 1058 912 754 681 624 462 335 318 219 191 117 52 50 46 SfiI GGCCn_nnn'nGGCC Cuts at: 4906 4906 Size: 8425 SfoI GGC'GCC Cuts at: 2044 3503 3581 3678 5377 2044 Size: 1459 78 97 1699 5092 Fragments arranged by size: 5092 1699 1459 97 78 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8425 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8425 SmaI CCC'GGG Cuts at: 1872 4626 5204 5752 1872 Size: 2754 578 548 4545 Fragments arranged by size: 4545 2754 578 548 SmlI C'TyrA_G Cuts at: 820 1017 1943 3401 4568 4589 5111 5648 6529 Size: 197 926 1458 1167 21 522 537 881 Cuts at: 6529 7430 7692 820 Size: 901 262 1553 Fragments arranged by size: 1553 1458 1167 926 901 881 537 522 262 197 21 SnaBI TAC'GTA Cuts at: 493 5722 6216 493 Size: 5229 494 2702 Fragments arranged by size: 5229 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8425 SphI G_CATG'C Cuts at: 1132 5783 1132 Size: 4651 3774 SspI AAT'ATT Cuts at: 5 52 6349 5 Size: 47 6297 2081 Fragments arranged by size: 6297 2081 47 StuI AGG'CCT Cuts at: 1517 4676 4859 1517 Size: 3159 183 5083 Fragments arranged by size: 5083 3159 183 StyI C'CwwG_G Cuts at: 513 1065 2022 4455 4866 4872 5553 5864 513 Size: 552 957 2433 411 6 681 311 3074 Fragments arranged by size: 3074 2433 957 681 552 411 311 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2261 2683 4226 4448 4621 4951 5077 Size: 1255 681 422 1543 222 173 330 126 Cuts at: 5077 6452 6469 6622 6807 7252 325 Size: 1375 17 153 185 445 1498 Fragments arranged by size: 1543 1498 1375 1255 681 445 422 330 222 185 173 153 126 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2763 2829 6671 Size: 274 80 33 51 1443 786 66 3842 Cuts at: 6671 8383 96 Size: 1712 138 Fragments arranged by size: 3842 1712 1443 786 274 138 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5029 5061 5626 5658 8385 8417 5029 Size: 32 565 32 2727 32 5037 Fragments arranged by size: 5037 2727 565 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8425 XbaI T'CTAG_A Cuts at: 2722 5761 2722 Size: 3039 5386 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4555 5008 4555 Size: 453 7972 XhoI C'TCGA_G Cuts at: 1943 4568 5648 1943 Size: 2625 1080 4720 Fragments arranged by size: 4720 2625 1080 XmaI C'CCGG_G Cuts at: 1870 4624 5202 5750 1870 Size: 2754 578 548 4545 Fragments arranged by size: 4545 2754 578 548 XmnI GAAnn'nnTTC Cuts at: 6554 6554 Size: 8425 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6233 276 Size: 53 83 186 936 4699 2468 Fragments arranged by size: 4699 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI FseI FspAI NruI NsiI PacI PsrI RsrII SrfI SwaI