(Circular) (Six-base) MAPSORT of: pFN21ASDA1219.seq Check: 2551 from: 1 to: 9202 pFN21ASDA1219 9202 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2140 2140 Size: 9202 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3263 7012 278 Size: 53 83 186 936 1727 3749 2468 Fragments arranged by size: 3749 2468 1727 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5401 6545 1937 Size: 3464 1144 4594 Fragments arranged by size: 4594 3464 1144 Acc65I G'GTAC_C Cuts at: 2159 6523 2159 Size: 4364 4838 AclI AA'CG_TT Cuts at: 1563 7329 7702 1563 Size: 5766 373 3063 Fragments arranged by size: 5766 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2151 4016 4178 4728 5118 5187 5399 5745 Size: 519 1865 162 550 390 69 212 346 Cuts at: 5745 7265 8649 8917 1632 Size: 1520 1384 268 1917 Fragments arranged by size: 1917 1865 1520 1384 550 519 390 346 268 212 162 69 AflII C'TTAA_G Cuts at: 820 1017 4255 4580 820 Size: 197 3238 325 5442 Fragments arranged by size: 5442 3238 325 197 AflIII A'CryG_T Cuts at: 1203 2886 8101 9078 1203 Size: 1683 5215 977 1327 Fragments arranged by size: 5215 1683 1327 977 AgeI A'CCGG_T Cuts at: 8033 8033 Size: 9202 AhdI GACnn_n'nnGTC Cuts at: 7931 7931 Size: 9202 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9202 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9202 AlwNI CAG_nnn'CTG Cuts at: 2174 2455 2473 4277 6945 8517 2174 Size: 281 18 1804 2668 1572 2859 Fragments arranged by size: 2859 2668 1804 1572 281 18 ApaLI G'TGCA_C Cuts at: 2748 4441 7259 8415 2748 Size: 1693 2818 1156 3535 Fragments arranged by size: 3535 2818 1693 1156 ApoI r'AATT_y Cuts at: 1493 2185 2942 3533 3821 5321 5591 5895 6228 Size: 692 757 591 288 1500 270 304 333 Cuts at: 6228 6248 6511 6737 6889 8998 1493 Size: 20 263 226 152 2109 1697 Fragments arranged by size: 2109 1697 1500 757 692 591 333 304 288 270 263 226 152 20 AseI AT'TA_AT Cuts at: 160 1032 3867 4019 5673 7756 160 Size: 872 2835 152 1654 2083 1606 Fragments arranged by size: 2835 2083 1654 1606 872 152 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9202 AvaI C'yCGr_G Cuts at: 1870 1943 2499 6527 1870 Size: 73 556 4028 4545 Fragments arranged by size: 4545 4028 556 73 BamHI G'GATC_C Cuts at: 6532 6899 6532 Size: 367 8835 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2159 3177 4025 4332 6523 Size: 325 217 627 372 1018 848 307 2191 Cuts at: 6523 7978 618 Size: 1455 1842 Fragments arranged by size: 2191 1842 1455 1018 848 627 372 325 307 217 BanII G_rGCy'C Cuts at: 1391 4218 4284 4371 6301 6521 1391 Size: 2827 66 87 1930 220 4072 Fragments arranged by size: 4072 2827 1930 220 87 66 BbeI G_GCGC'C Cuts at: 4336 4336 Size: 9202 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3218 3611 4966 5553 928 Size: 971 1319 393 1355 587 4577 Fragments arranged by size: 4577 1355 1319 971 587 393 BbvCI CC'TCA_GC Cuts at: 3564 3564 Size: 9202 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2801 2835 4285 4319 7393 7427 1903 Size: 34 864 34 1450 34 3074 34 3678 Fragments arranged by size: 3678 3074 1450 864 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3006 7094 8304 3006 Size: 4088 1210 3904 Fragments arranged by size: 4088 3904 1210 BclI T'GATC_A Cuts at: 1548 2065 1548 Size: 517 8685 BfrBI ATG'CAT Cuts at: 3065 4725 3065 Size: 1660 7542 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7813 136 Size: 107 122 71 631 605 6141 1525 Fragments arranged by size: 6141 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5131 6155 9197 5131 Size: 1024 3042 5136 Fragments arranged by size: 5136 3042 1024 BlpI GC'TnA_GC Cuts at: 6619 6619 Size: 9202 Bme1580I G_kGCm'C Cuts at: 1790 2752 4445 4975 7263 8419 1790 Size: 962 1693 530 2288 1156 2573 Fragments arranged by size: 2573 2288 1693 1156 962 530 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9202 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4321 4616 5036 Size: 416 53 83 399 24 2904 295 420 Cuts at: 5036 5279 5655 5970 6933 7891 442 Size: 243 376 315 963 958 1753 Fragments arranged by size: 2904 1753 963 958 420 416 399 376 315 295 243 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3459 5742 1056 Size: 2403 2283 4516 Fragments arranged by size: 4516 2403 2283 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4260 4390 4260 Size: 130 9072 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2722 2820 3630 4471 4613 4798 6458 7862 Size: 1483 98 810 841 142 185 1660 1404 Cuts at: 7862 1239 Size: 2579 Fragments arranged by size: 2579 1660 1483 1404 841 810 185 142 98 Bpu10I CC'TnA_GC Cuts at: 3564 3564 Size: 9202 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4230 5258 5942 6464 7327 8192 8490 4230 Size: 1028 684 522 863 865 298 4942 Fragments arranged by size: 4942 1028 865 863 684 522 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3325 4231 4407 4787 7865 882 Size: 617 1826 906 176 380 3078 2219 Fragments arranged by size: 3078 2219 1826 906 617 380 176 BsaAI yAC'GTr Cuts at: 493 1204 6499 6993 493 Size: 711 5295 494 2702 Fragments arranged by size: 5295 2702 711 494 BsaBI GATnn'nnATC Cuts at: 9196 9196 Size: 9202 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3260 4333 7009 7391 Size: 53 83 186 936 1727 1073 2676 382 Cuts at: 7391 8026 275 Size: 635 1451 Fragments arranged by size: 2676 1727 1451 1073 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3171 4416 4845 6219 6423 7635 8033 8307 8454 Size: 1245 429 1374 204 1212 398 274 147 Cuts at: 8454 8865 3171 Size: 411 3508 Fragments arranged by size: 3508 1374 1245 1212 429 411 398 274 204 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2253 2283 6431 6461 1178 Size: 30 165 30 850 30 4148 30 3919 Fragments arranged by size: 4148 3919 850 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3264 3714 4361 5013 5267 5397 1186 Size: 2078 450 647 652 254 130 4991 Fragments arranged by size: 4991 2078 652 647 450 254 130 BseYI C'CCAG_C Cuts at: 2713 2917 4277 5211 8405 8901 2713 Size: 204 1360 934 3194 496 3014 Fragments arranged by size: 3194 3014 1360 934 496 204 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2072 4481 1163 Size: 573 336 2409 5884 Fragments arranged by size: 5884 2409 573 336 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6652 7413 8441 1540 Size: 275 186 4651 761 1028 2301 Fragments arranged by size: 4651 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2752 3103 4371 4445 4551 6301 7263 7348 8419 Size: 351 1268 74 106 1750 962 85 1071 Cuts at: 8419 2752 Size: 3535 Fragments arranged by size: 3535 1750 1268 1071 962 351 106 85 74 BsmI GAATG_Cn' Cuts at: 2979 3065 4296 5139 6713 6806 2979 Size: 86 1231 843 1574 93 5375 Fragments arranged by size: 5375 1574 1231 843 93 86 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2752 3103 4218 4284 4371 4445 4551 Size: 399 962 351 1115 66 87 74 106 Cuts at: 4551 4975 6301 6521 7263 7348 8419 1391 Size: 424 1326 220 742 85 1071 2174 Fragments arranged by size: 2174 1326 1115 1071 962 742 424 399 351 220 106 87 85 74 66 BspEI T'CCGG_A Cuts at: 6219 8865 6219 Size: 2646 6556 BspHI T'CATG_A Cuts at: 7091 8821 7091 Size: 1730 7472 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2140 4249 5395 6557 844 Size: 1296 2109 1146 1162 3489 Fragments arranged by size: 3489 2109 1296 1162 1146 BsrBI CCG'CTC Cuts at: 1394 1823 2970 7089 1394 Size: 429 1147 4119 3507 Fragments arranged by size: 4119 3507 1147 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2336 2580 4083 4887 7697 7871 65 Size: 1183 1088 244 1503 804 2810 174 1396 Fragments arranged by size: 2810 1503 1396 1183 1088 804 244 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7846 8033 1700 Size: 110 6036 187 2869 Fragments arranged by size: 6036 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9202 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9202 BssSI C'ACGA_G Cuts at: 7262 8274 7262 Size: 1012 8190 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9202 BstBI TT'CG_AA Cuts at: 2007 8006 2007 Size: 5999 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9202 BstXI CCAn_nnnn'nTGG Cuts at: 2689 4510 6473 2689 Size: 1821 1963 5418 Fragments arranged by size: 5418 1963 1821 BstYI r'GATC_y Cuts at: 1972 3857 4492 5131 5467 5696 5881 6155 6532 Size: 1885 635 639 336 229 185 274 377 Cuts at: 6532 6899 7284 7301 8753 8839 8851 9197 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1885 1452 639 635 385 377 367 346 336 274 229 185 86 17 12 Bsu36I CC'TnA_GG Cuts at: 6000 6000 Size: 9202 BtgI C'CryG_G Cuts at: 513 1065 2220 3501 513 Size: 552 1155 1281 6214 Fragments arranged by size: 6214 1281 1155 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8463 Fragments arranged by size: 8463 683 56 BtsI GCAGTG_nn' Cuts at: 2042 2724 2735 3314 6718 7512 7532 2042 Size: 682 11 579 3404 794 20 3712 Fragments arranged by size: 3712 3404 794 682 579 20 11 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9202 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9167 DraI TTT'AAA Cuts at: 2803 5068 5547 6204 6506 2803 Size: 2265 479 657 302 5499 Fragments arranged by size: 5499 2265 657 479 302 DraIII CAC_nnn'GTG Cuts at: 4760 4760 Size: 9202 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8209 809 Size: 507 6893 1802 Fragments arranged by size: 6893 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3311 3702 4469 Size: 54 1425 50 275 18 1481 391 767 Cuts at: 4469 5087 6649 7538 9167 8 Size: 618 1562 889 1629 43 Fragments arranged by size: 1629 1562 1481 1425 889 767 618 391 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6649 1537 Size: 275 4837 4090 Fragments arranged by size: 4837 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3553 3609 3967 4154 5094 5172 5261 7132 Size: 2200 56 358 187 940 78 89 1871 Cuts at: 7132 1353 Size: 3423 Fragments arranged by size: 3423 2200 1871 940 358 187 89 78 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6374 7789 8163 8309 387 Size: 1064 138 4785 1415 374 146 1280 Fragments arranged by size: 4785 1415 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4369 6299 4369 Size: 1930 7272 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2151 2722 2820 3630 4016 4178 4471 Size: 393 519 571 98 810 386 162 293 Cuts at: 4471 4613 4728 4798 5118 5187 5399 5745 6458 Size: 142 115 70 320 69 212 346 713 Cuts at: 6458 7265 7862 8649 8917 1239 Size: 807 597 787 268 1524 Fragments arranged by size: 1524 810 807 787 713 597 571 519 393 386 346 320 293 268 212 162 142 115 98 70 69 EcoNI CCTnn'n_nnAGG Cuts at: 4385 5962 4385 Size: 1577 7625 EcoO109I rG'GnC_Cy Cuts at: 2452 2452 Size: 9202 EcoRI G'AATT_C Cuts at: 5321 5895 6511 6889 5321 Size: 574 616 378 7634 Fragments arranged by size: 7634 616 574 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4983 4983 Size: 9202 FspI TGC'GCA Cuts at: 7708 7708 Size: 9202 HaeII r_GCGC'y Cuts at: 1678 4336 8349 1678 Size: 2658 4013 2531 Fragments arranged by size: 4013 2658 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2024 2056 3944 3976 4153 Size: 32 135 32 452 32 1888 32 177 Cuts at: 4153 4185 4484 4516 5956 5988 7843 7875 7917 Size: 32 299 32 1440 32 1855 32 42 Cuts at: 7917 7949 1373 Size: 32 2626 Fragments arranged by size: 2626 1888 1855 1440 452 299 177 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3343 4405 6415 6546 6792 669 Size: 988 281 1405 1062 2010 131 246 3079 Fragments arranged by size: 3079 2010 1405 1062 988 281 246 131 HindIII A'AGCT_T Cuts at: 748 5018 748 Size: 4270 4932 HpaI GTT'AAC Cuts at: 3343 6792 3343 Size: 3449 5753 KasI G'GCGC_C Cuts at: 4332 4332 Size: 9202 KpnI G_GTAC'C Cuts at: 2163 6527 2163 Size: 4364 4838 MfeI C'AATT_G Cuts at: 6801 6801 Size: 9202 MluI A'CGCG_T Cuts at: 9078 9078 Size: 9202 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2089 3866 5041 5621 6454 8316 8500 1305 Size: 784 1777 1175 580 833 1862 184 2007 Fragments arranged by size: 2007 1862 1777 1175 833 784 580 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3704 10 Size: 54 1425 343 1872 5508 Fragments arranged by size: 5508 1872 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2079 2866 4061 4508 7160 7519 7678 Size: 648 913 787 1195 447 2652 359 159 Cuts at: 7678 518 Size: 2042 Fragments arranged by size: 2652 2042 1195 913 787 648 447 359 159 MspA1I CmG'CkG Cuts at: 1606 3051 4274 6436 6618 7295 8443 8688 1606 Size: 1445 1223 2162 182 677 1148 245 2120 Fragments arranged by size: 2162 2120 1445 1223 1148 677 245 182 NarI GG'CG_CC Cuts at: 4333 4333 Size: 9202 NcoI C'CATG_G Cuts at: 513 1065 2220 3501 513 Size: 552 1155 1281 6214 Fragments arranged by size: 6214 1281 1155 552 NdeI CA'TA_TG Cuts at: 387 2779 387 Size: 2392 6810 NheI G'CTAG_C Cuts at: 1052 3455 5738 1052 Size: 2403 2283 4516 Fragments arranged by size: 4516 2403 2283 NotI GC'GGCC_GC Cuts at: 6649 6649 Size: 9202 NsiI A_TGCA'T Cuts at: 3067 4727 3067 Size: 1660 7542 NspI r_CATG'y Cuts at: 1132 1229 2890 3652 4725 5654 6560 8105 1132 Size: 97 1661 762 1073 929 906 1545 2229 Fragments arranged by size: 2229 1661 1545 1073 929 906 762 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9202 PciI A'CATG_T Cuts at: 2886 8101 2886 Size: 5215 3987 PflMI CCAn_nnn'nTGG Cuts at: 1108 2258 1108 Size: 1150 8052 PfoI T'CCnGG_A Cuts at: 3930 6465 3930 Size: 2535 6667 PmeI GTTT'AAAC Cuts at: 6506 6506 Size: 9202 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7271 7303 8784 8816 7271 Size: 32 1481 32 7657 Fragments arranged by size: 7657 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2452 2452 Size: 9202 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9202 PsiI TTA'TAA Cuts at: 2927 6772 2927 Size: 3845 5357 PstI C_TGCA'G Cuts at: 830 2538 6491 6554 830 Size: 1708 3953 63 3478 Fragments arranged by size: 3953 3478 1708 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9202 PvuII CAG'CTG Cuts at: 3051 4274 6436 3051 Size: 1223 2162 5817 Fragments arranged by size: 5817 2162 1223 SacI G_AGCT'C Cuts at: 4371 6301 4371 Size: 1930 7272 SalI G'TCGA_C Cuts at: 1936 6544 1936 Size: 4608 4594 SapI GCTCTTCn'nnn_ Cuts at: 3553 3967 3553 Size: 414 8788 SbfI CC_TGCA'GG Cuts at: 6554 6554 Size: 9202 ScaI AGT'ACT Cuts at: 3229 4590 6115 7450 9162 3229 Size: 1361 1525 1335 1712 3269 Fragments arranged by size: 3269 1712 1525 1361 1335 SfcI C'TryA_G Cuts at: 826 1045 2130 2390 2534 6045 6367 6487 6550 Size: 219 1085 260 144 3511 322 120 63 Cuts at: 6550 7685 8366 8557 826 Size: 1135 681 191 1471 Fragments arranged by size: 3511 1471 1135 1085 681 322 260 219 191 144 120 63 SfoI GGC'GCC Cuts at: 4334 4334 Size: 9202 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9202 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9202 SmaI CCC'GGG Cuts at: 1872 6529 1872 Size: 4657 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4245 4255 4580 5237 5921 6479 Size: 197 926 2302 10 325 657 684 558 Cuts at: 6479 7306 8207 8469 820 Size: 827 901 262 1553 Fragments arranged by size: 2302 1553 926 901 827 684 657 558 325 262 197 10 SnaBI TAC'GTA Cuts at: 493 6499 6993 493 Size: 6006 494 2702 Fragments arranged by size: 6006 2702 494 SpeI A'CTAG_T Cuts at: 152 3296 152 Size: 3144 6058 SphI G_CATG'C Cuts at: 1132 6560 1132 Size: 5428 3774 SspI AAT'ATT Cuts at: 5 52 2276 3024 4087 5072 7126 5 Size: 47 2224 748 1063 985 2054 2081 Fragments arranged by size: 2224 2081 2054 1063 985 748 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9202 StyI C'CwwG_G Cuts at: 513 1065 2220 2682 3501 5605 6022 6641 513 Size: 552 1155 462 819 2104 417 619 3074 Fragments arranged by size: 3074 2104 1155 819 619 552 462 417 SwaI ATTT'AAAT Cuts at: 5068 5068 Size: 9202 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2727 4073 4127 4325 6043 7229 7246 Size: 1255 1147 1346 54 198 1718 1186 17 Cuts at: 7246 7399 7584 8029 325 Size: 153 185 445 1498 Fragments arranged by size: 1718 1498 1346 1255 1186 1147 445 198 185 153 54 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2025 2642 3227 Size: 274 80 33 51 1443 48 617 585 Cuts at: 3227 4588 4653 6113 7448 9160 96 Size: 1361 65 1460 1335 1712 138 Fragments arranged by size: 1712 1460 1443 1361 1335 617 585 274 138 80 65 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2103 2135 2837 2869 3245 3277 9162 9194 2103 Size: 32 702 32 376 32 5885 32 2111 Fragments arranged by size: 5885 2111 702 376 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6410 1316 Size: 5094 4108 XbaI T'CTAG_A Cuts at: 3080 4363 6538 3080 Size: 1283 2175 5744 Fragments arranged by size: 5744 2175 1283 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2470 2689 3499 2470 Size: 219 810 8173 Fragments arranged by size: 8173 810 219 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9202 XmaI C'CCGG_G Cuts at: 1870 6527 1870 Size: 4657 4545 XmnI GAAnn'nnTTC Cuts at: 3618 6081 7331 3618 Size: 2463 1250 5489 Fragments arranged by size: 5489 2463 1250 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3261 7010 276 Size: 53 83 186 936 1727 3749 2468 Fragments arranged by size: 3749 2468 1727 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI