Restriction map for
pFN21ASDA1100
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI Ksp632I KspI MamI
MboII MfeI MflI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tsp509I Tth111I Van91I XbaI XhoII
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 1166 CGCG CG^CG
AfaI 1095 GTAC GT^AC
AflIII 1246 ACGTGT A^C[GA][CT]GT
AlwI 769 GATCC GGATC(4/5)
Aor51HI 152 AGCGCT AGC^GCT
BbeI 591 GGCGCC GGCGC^C
BfaI 26 CTAG C^TAG
BsaI 1060 GGTCTC GGTCTC(1/5)
BsaWI 1241 ACCGGA [AT]^CCGG[AT]
BseRI 202 GAGGAG GAGGAG(10/8)
BsmI 242 GAATGC GAATGC(1/-1)
BsrBI 1033 GAGCGG CCGCTC(-3/-3)
BsrDI 952 GCAATG GCAATG(2/0)
BsrI 530 CCAGT ACTGG(1/-1)
BssSI 289 CACGAG CACGAG(-5/-1)
BstEII 330 GGTCACC G^GT[ACGT]ACC
BstPI 330 GGTCACC G^GT[ACGT]ACC
BstUI 1166 CGCG CG^CG
DraIII 1209 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 152 AGCGCT AGC^GCT
EcoO65I 330 GGTCACC G^GT[ACGT]ACC
HincII 1219 GTTGAC GT[CT]^[GA]AC
HindII 1219 GTTGAC GT[CT]^[GA]AC
KasI 591 GGCGCC G^GCGCC
MaeI 26 CTAG C^TAG
MseI 1103 TTAA T^TAA
MvnI 1166 CGCG CG^CG
NaeI 226 GCCGGC GCC^GGC
NarI 591 GGCGCC GG^CGCC
NcoI 7 CCATGG C^CATGG
NgoMIV 226 GCCGGC G^CCGGC
NspI 409 GCATGC [GA]CATG^[CT]
PaeR7I 115 CTCGAG C^TCGAG
PleI 1181 GAGTC GAGTC(4/5)
RsaI 1095 GTAC GT^AC
SfiI 112 GGCCTCGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 591 GGCGCC GGC^GCC
SmlI 115 CTCGAG C^T[CT][GA]AG
SphI 409 GCATGC GCATG^C
StuI 509 AGGCCT AGG^CCT
TfiI 62 GATTC G^A[AT]TC
Tru9I 1103 TTAA T^TAA
XhoI 115 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 591 GGCGCC G[GA]^CG[CT]C
1267 GACGCC
Alw44I 298 GTGCAC G^TGCAC
477 GTGCAC
ApaI 120 GGGCCC GGGCC^C
958 GGGCCC
ApaLI 298 GTGCAC G^TGCAC
477 GTGCAC
BalI 31 TGGCCA TGG^CCA
128 TGGCCA
BglI 113 GCCTCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
594 GCCCCTGGGGC
BpmI 528 CTCCAG CTGGAG(16/14)
643 CTCCAG
BsaHI 591 GGCGCC G[GA]^CG[CT]C
1267 GACGCC
BsrFI 226 GCCGGC [GA]^CCGG[CT]
561 ACCGGC
BstXI 581 CCACCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
965 CCATCAGCCTGG
Cfr10I 226 GCCGGC [GA]^CCGG[CT]
561 ACCGGC
DdeI 829 CTGAG C^T[ACGT]AG
1292 CTGAG
DpnII 283 GATC ^GATC
769 GATC
EcoT14I 7 CCATGG C^C[AT][AT]GG
348 CCAAGG
HgaI 1131 GACGC GACGC(5/10)
1267 GACGC
Hin1I 591 GGCGCC G[GA]^CG[CT]C
1267 GACGCC
HinfI 62 GATTC G^A[ACGT]TC
1181 GAGTC
MboI 283 GATC ^GATC
769 GATC
MluNI 31 TGGCCA TGG^CCA
128 TGGCCA
MscI 31 TGGCCA TGG^CCA
128 TGGCCA
MslI 333 CACCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
582 CACCCACATG
NdeII 283 GATC ^GATC
769 GATC
PpuMI 72 GGGACCT [GA]G^G[AT]CC[CT]
306 GGGACCT
PvuII 490 CAGCTG CAG^CTG
1171 CAGCTG
Sau3AI 283 GATC ^GATC
769 GATC
StyI 7 CCATGG C^C[AT][AT]GG
348 CCAAGG
XcmI 372 CCAGCACATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
962 CCACCATCAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BssKI BstAPI BstF5I BstNI
Cac8I CfoI Cfr13I DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PstI
Sau96I ScrFI SfaNI SfcI SmaI
TaqI TseI Tsp45I TspRI TthHB8I
XmaCI XmaI