(Circular) (Six-base) MAPSORT of: pFN21ASDA1100.seq Check: 535 from: 1 to: 6025 pFN21ASDA1100 6025 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2308 2308 Size: 6025 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3835 278 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3368 1937 Size: 1431 4594 Acc65I G'GTAC_C Cuts at: 3346 3346 Size: 6025 AclI AA'CG_TT Cuts at: 1563 4152 4525 1563 Size: 2589 373 3063 Fragments arranged by size: 3063 2589 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3157 4088 5472 5740 1632 Size: 1525 931 1384 268 1917 Fragments arranged by size: 1917 1525 1384 931 268 AfeI AGC'GCT Cuts at: 2177 2177 Size: 6025 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5828 AflIII A'CryG_T Cuts at: 1203 3269 4924 5901 1203 Size: 2066 1655 977 1327 Fragments arranged by size: 2066 1655 1327 977 AgeI A'CCGG_T Cuts at: 4856 4856 Size: 6025 AhdI GACnn_n'nnGTC Cuts at: 4754 4754 Size: 6025 AleI CACnn'nnGTG Cuts at: 1166 2360 1166 Size: 1194 4831 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6025 AlwNI CAG_nnn'CTG Cuts at: 2515 2809 2884 3157 3768 5340 2515 Size: 294 75 273 611 1572 3200 Fragments arranged by size: 3200 1572 611 294 273 75 ApaI G_GGCC'C Cuts at: 2147 2985 2147 Size: 838 5187 ApaLI G'TGCA_C Cuts at: 2321 2500 4082 5238 2321 Size: 179 1582 1156 3108 Fragments arranged by size: 3108 1582 1156 179 ApoI r'AATT_y Cuts at: 1493 3334 3560 3712 5821 1493 Size: 1841 226 152 2109 1697 Fragments arranged by size: 2109 1841 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4579 160 Size: 872 3547 1606 Fragments arranged by size: 3547 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 AvaI C'yCGr_G Cuts at: 1870 1943 2110 2138 2166 3078 3350 1870 Size: 73 167 28 28 912 272 4545 Fragments arranged by size: 4545 912 272 167 73 28 28 BamHI G'GATC_C Cuts at: 3355 3722 3355 Size: 367 5658 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2614 2767 3301 3346 4801 Size: 325 217 627 827 153 534 45 1455 Cuts at: 4801 618 Size: 1842 Fragments arranged by size: 1842 1455 827 627 534 325 217 153 45 BanII G_rGCy'C Cuts at: 1391 2038 2147 2261 2294 2985 3344 1391 Size: 647 109 114 33 691 359 4072 Fragments arranged by size: 4072 691 647 359 114 109 33 BbeI G_GCGC'C Cuts at: 2618 2618 Size: 6025 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5054 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4216 4250 1903 Size: 34 2279 34 3678 Fragments arranged by size: 3678 2279 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3917 5127 3917 Size: 1210 4815 BclI T'GATC_A Cuts at: 1548 1548 Size: 6025 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2142 2623 4636 Size: 107 122 71 631 605 470 481 2013 Cuts at: 4636 136 Size: 1525 Fragments arranged by size: 2013 1525 631 605 481 470 122 107 71 BglII A'GATC_T Cuts at: 6020 6020 Size: 6025 BlpI GC'TnA_GC Cuts at: 3442 3442 Size: 6025 Bme1580I G_kGCm'C Cuts at: 1790 2147 2325 2419 2504 2526 2772 2985 3306 Size: 357 178 94 85 22 246 213 321 Cuts at: 3306 4086 5242 1790 Size: 780 1156 2573 Fragments arranged by size: 2573 1156 780 357 321 246 213 178 94 85 22 BmgBI CAC'GTC Cuts at: 1318 2686 3007 3270 1318 Size: 1368 321 263 4073 Fragments arranged by size: 4073 1368 321 263 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3756 4714 442 Size: 416 53 83 399 24 2339 958 1753 Fragments arranged by size: 2339 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6025 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2536 2651 4685 1239 Size: 1297 115 2034 2579 Fragments arranged by size: 2579 2034 1297 115 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4150 5015 5313 4150 Size: 865 298 4862 Fragments arranged by size: 4862 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3089 4688 882 Size: 617 1590 1599 2219 Fragments arranged by size: 2219 1599 1590 617 BsaAI yAC'GTr Cuts at: 493 1204 3322 3816 493 Size: 711 2118 494 2702 Fragments arranged by size: 2702 2118 711 494 BsaBI GATnn'nnATC Cuts at: 6019 6019 Size: 6025 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2615 3291 3832 4214 Size: 53 83 186 936 1082 676 541 382 Cuts at: 4214 4849 275 Size: 635 1451 Fragments arranged by size: 1451 1082 936 676 635 541 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3264 4458 4856 5130 5277 5688 3264 Size: 1194 398 274 147 411 3601 Fragments arranged by size: 3601 1194 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2534 2564 2693 2723 3302 Size: 30 165 30 1131 30 129 30 579 Cuts at: 3302 3332 1178 Size: 30 3871 Fragments arranged by size: 3871 1131 579 165 129 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2240 1186 Size: 1054 4971 BseYI C'CCAG_C Cuts at: 2187 2275 2283 2418 2428 2598 2646 2749 2927 Size: 88 8 135 10 170 48 103 178 Cuts at: 2927 3061 5228 5724 2187 Size: 134 2167 496 2488 Fragments arranged by size: 2488 2167 496 178 170 135 134 103 88 48 10 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2255 2555 2759 2783 1163 Size: 573 519 300 204 24 4405 Fragments arranged by size: 4405 573 519 300 204 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3475 4236 5264 1540 Size: 275 186 1474 761 1028 2301 Fragments arranged by size: 2301 1474 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2325 2504 2858 4086 4171 5242 2325 Size: 179 354 1228 85 1071 3108 Fragments arranged by size: 3108 1228 1071 354 179 85 BsmI GAATG_Cn' Cuts at: 2271 3536 3629 2271 Size: 1265 93 4667 Fragments arranged by size: 4667 1265 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 2147 2261 2294 2325 2419 2504 Size: 399 248 109 114 33 31 94 85 Cuts at: 2504 2526 2772 2858 2985 3306 3344 4086 4171 Size: 22 246 86 127 321 38 742 85 Cuts at: 4171 5242 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 321 248 246 127 114 109 94 86 85 85 38 33 31 22 BspEI T'CCGG_A Cuts at: 5688 5688 Size: 6025 BspHI T'CATG_A Cuts at: 3914 5644 3914 Size: 1730 4295 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2308 2700 2790 3380 844 Size: 1464 392 90 590 3489 Fragments arranged by size: 3489 1464 590 392 90 BsrBI CCG'CTC Cuts at: 1394 1823 3058 3912 1394 Size: 429 1235 854 3507 Fragments arranged by size: 3507 1235 854 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2982 4520 4694 65 Size: 1183 1734 1538 174 1396 Fragments arranged by size: 1734 1538 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2249 2584 4669 4856 1700 Size: 110 439 335 2085 187 2869 Fragments arranged by size: 2869 2085 439 335 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6025 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6025 BssSI C'ACGA_G Cuts at: 2312 4085 5097 2312 Size: 1773 1012 3240 Fragments arranged by size: 3240 1773 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2515 2782 2884 794 Size: 1721 267 102 3935 Fragments arranged by size: 3935 1721 267 102 BstBI TT'CG_AA Cuts at: 2007 4829 2007 Size: 2822 3203 BstEII G'GTnAC_C Cuts at: 1184 2353 1184 Size: 1169 4856 BstXI CCAn_nnnn'nTGG Cuts at: 2611 2995 2611 Size: 384 5641 BstYI r'GATC_y Cuts at: 1972 3355 3722 4107 4124 5576 5662 5674 6020 Size: 1383 367 385 17 1452 86 12 346 Cuts at: 6020 1972 Size: 1977 Fragments arranged by size: 1977 1452 1383 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2030 2070 2843 513 Size: 552 965 40 773 3695 Fragments arranged by size: 3695 965 773 552 40 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5286 Fragments arranged by size: 5286 683 56 BtsI GCAGTG_nn' Cuts at: 2049 2419 2882 3541 4335 4355 2049 Size: 370 463 659 794 20 3719 Fragments arranged by size: 3719 794 659 463 370 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6025 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5990 DraI TTT'AAA Cuts at: 3329 3329 Size: 6025 DraIII CAC_nnn'GTG Cuts at: 3237 3237 Size: 6025 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5032 809 Size: 507 3716 1802 Fragments arranged by size: 3716 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2054 2151 3051 Size: 54 1425 50 275 18 224 97 900 Cuts at: 3051 3472 4361 5990 8 Size: 421 889 1629 43 Fragments arranged by size: 1629 1425 900 889 421 275 224 97 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3472 1537 Size: 275 1660 4090 Fragments arranged by size: 4090 1660 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3955 1353 Size: 2602 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2053 4612 4986 5132 387 Size: 1064 138 464 2559 374 146 1280 Fragments arranged by size: 2559 1280 1064 464 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2536 2651 3157 4088 4685 5472 5740 Size: 393 904 115 506 931 597 787 268 Cuts at: 5740 1239 Size: 1524 Fragments arranged by size: 1524 931 904 787 597 506 393 268 115 EcoO109I rG'GnC_Cy Cuts at: 2096 2143 2144 2330 2981 3037 2096 Size: 47 1 186 651 56 5084 Fragments arranged by size: 5084 651 186 56 47 1 EcoRI G'AATT_C Cuts at: 3334 3712 3334 Size: 378 5647 FspI TGC'GCA Cuts at: 4531 4531 Size: 6025 HaeII r_GCGC'y Cuts at: 1678 2179 2233 2618 5172 1678 Size: 501 54 385 2554 2531 Fragments arranged by size: 2554 2531 501 385 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2693 2725 4666 4698 4740 Size: 32 135 32 1121 32 1941 32 42 Cuts at: 4740 4772 1373 Size: 32 2626 Fragments arranged by size: 2626 1941 1121 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3244 3369 3615 669 Size: 988 281 1306 125 246 3079 Fragments arranged by size: 3079 1306 988 281 246 125 HindIII A'AGCT_T Cuts at: 748 748 Size: 6025 HpaI GTT'AAC Cuts at: 3615 3615 Size: 6025 KasI G'GCGC_C Cuts at: 2614 2614 Size: 6025 KpnI G_GTAC'C Cuts at: 3350 3350 Size: 6025 MfeI C'AATT_G Cuts at: 3624 3624 Size: 6025 MluI A'CGCG_T Cuts at: 5901 5901 Size: 6025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3126 5139 5323 1305 Size: 1821 2013 184 2007 Fragments arranged by size: 2013 2007 1821 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2056 2153 10 Size: 54 1425 343 224 97 3882 Fragments arranged by size: 3882 1425 343 224 97 54 MslI CAynn'nnrTG Cuts at: 518 1166 2360 2609 3983 4342 4501 518 Size: 648 1194 249 1374 359 159 2042 Fragments arranged by size: 2042 1374 1194 648 359 249 159 MspA1I CmG'CkG Cuts at: 1606 2080 2515 2671 2797 3196 3441 4118 5266 Size: 474 435 156 126 399 245 677 1148 Cuts at: 5266 5511 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 474 435 399 245 245 156 126 NaeI GCC'GGC Cuts at: 2251 2251 Size: 6025 NarI GG'CG_CC Cuts at: 2615 2615 Size: 6025 NcoI C'CATG_G Cuts at: 513 1065 2030 513 Size: 552 965 4508 Fragments arranged by size: 4508 965 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6025 NgoMIV G'CCGG_C Cuts at: 2249 2249 Size: 6025 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6025 NotI GC'GGCC_GC Cuts at: 3472 3472 Size: 6025 NspI r_CATG'y Cuts at: 1132 1229 2436 3383 4928 1132 Size: 97 1207 947 1545 2229 Fragments arranged by size: 2229 1545 1207 947 97 PasI CC'CwG_GG Cuts at: 1348 2620 2713 3306 1348 Size: 1272 93 593 4067 Fragments arranged by size: 4067 1272 593 93 PciI A'CATG_T Cuts at: 4924 4924 Size: 6025 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6025 PmeI GTTT'AAAC Cuts at: 3329 3329 Size: 6025 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4094 4126 5607 5639 4094 Size: 32 1481 32 4480 Fragments arranged by size: 4480 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2096 2330 2096 Size: 234 5791 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6025 PsiI TTA'TAA Cuts at: 3595 3595 Size: 6025 PspOMI G'GGCC_C Cuts at: 2143 2981 2143 Size: 838 5187 PspXI vC'TCGA_Gb Cuts at: 2138 2138 Size: 6025 PstI C_TGCA'G Cuts at: 830 2414 2643 2877 3377 830 Size: 1584 229 234 500 3478 Fragments arranged by size: 3478 1584 500 234 229 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6025 PvuII CAG'CTG Cuts at: 2515 3196 2515 Size: 681 5344 SalI G'TCGA_C Cuts at: 1936 3367 1936 Size: 1431 4594 SbfI CC_TGCA'GG Cuts at: 3377 3377 Size: 6025 ScaI AGT'ACT Cuts at: 4273 5985 4273 Size: 1712 4313 SfcI C'TryA_G Cuts at: 826 1045 2388 2410 2639 2873 3373 4508 5189 Size: 219 1343 22 229 234 500 1135 681 Cuts at: 5189 5380 826 Size: 191 1471 Fragments arranged by size: 1471 1343 1135 681 500 234 229 219 191 22 SfiI GGCCn_nnn'nGGCC Cuts at: 2142 2142 Size: 6025 SfoI GGC'GCC Cuts at: 2616 2616 Size: 6025 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6025 SmaI CCC'GGG Cuts at: 1872 2112 2168 3080 3352 1872 Size: 240 56 912 272 4545 Fragments arranged by size: 4545 912 272 240 56 SmlI C'TyrA_G Cuts at: 820 1017 1943 2138 4129 5030 5292 820 Size: 197 926 195 1991 901 262 1553 Fragments arranged by size: 1991 1553 926 901 262 197 195 SnaBI TAC'GTA Cuts at: 493 3322 3816 493 Size: 2829 494 2702 Fragments arranged by size: 2829 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6025 SphI G_CATG'C Cuts at: 1132 2436 3383 1132 Size: 1304 947 3774 Fragments arranged by size: 3774 1304 947 SspI AAT'ATT Cuts at: 5 52 3949 5 Size: 47 3897 2081 Fragments arranged by size: 3897 2081 47 StuI AGG'CCT Cuts at: 1517 2534 1517 Size: 1017 5008 StyI C'CwwG_G Cuts at: 513 1065 2030 2371 3464 513 Size: 552 965 341 1093 3074 Fragments arranged by size: 3074 1093 965 552 341 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2289 2297 2621 2873 4052 4069 4222 Size: 1255 709 8 324 252 1179 17 153 Cuts at: 4222 4407 4852 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1179 709 445 324 252 185 153 17 8 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4271 5983 96 Size: 274 80 33 51 1443 2294 1712 138 Fragments arranged by size: 2294 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2534 2566 5985 6017 2534 Size: 32 3419 32 2542 Fragments arranged by size: 3419 2542 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6025 XbaI T'CTAG_A Cuts at: 3361 3361 Size: 6025 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2402 2992 2402 Size: 590 5435 XhoI C'TCGA_G Cuts at: 1943 2138 1943 Size: 195 5830 XmaI C'CCGG_G Cuts at: 1870 2110 2166 3078 3350 1870 Size: 240 56 912 272 4545 Fragments arranged by size: 4545 912 272 240 56 XmnI GAAnn'nnTTC Cuts at: 4154 4154 Size: 6025 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3833 276 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PsrI RsrII SacI SacII SanDI SapI SexAI SrfI SwaI