Restriction map for
pFN21ASDA1100
(Linear) (Six-base) MAPSORT of: KIAA1100.seq Check: 8506 from: 1 to: 1296
KIAA1100.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 285 1296
Size: 285 1011
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1134 1296
Size: 1134 162
AfeI AGC'GCT
Cuts at: 0 154 1296
Size: 154 1142
AflIII A'CryG_T
Cuts at: 0 1246 1296
Size: 1246 50
AleI CACnn'nnGTG
Cuts at: 0 337 1296
Size: 337 959
AlwNI CAG_nnn'CTG
Cuts at: 0 492 786 861 1134 1296
Size: 492 294 75 273 162
Fragments arranged by size:
492 294 273 162 75
ApaI G_GGCC'C
Cuts at: 0 124 962 1296
Size: 124 838 334
Fragments arranged by size:
838 334 124
ApaLI G'TGCA_C
Cuts at: 0 298 477 1296
Size: 298 179 819
Fragments arranged by size:
819 298 179
AvaI C'yCGr_G
Cuts at: 0 87 115 143 1055 1296
Size: 87 28 28 912 241
Fragments arranged by size:
912 241 87 28 28
BanI G'GyrC_C
Cuts at: 0 591 744 1278 1296
Size: 591 153 534 18
Fragments arranged by size:
591 534 153 18
BanII G_rGCy'C
Cuts at: 0 15 124 238 271 962 1296
Size: 15 109 114 33 691 334
Fragments arranged by size:
691 334 114 109 33 15
BbeI G_GCGC'C
Cuts at: 0 595 1296
Size: 595 701
BglI GCCn_nnn'nGGC
Cuts at: 0 119 600 1296
Size: 119 481 696
Fragments arranged by size:
696 481 119
Bme1580I G_kGCm'C
Cuts at: 0 124 302 396 481 503 749 962 1283
Size: 124 178 94 85 22 246 213 321
Cuts at: 1283 1296
Size: 13
Fragments arranged by size:
321 246 213 178 124 94 85 22
13
BmgBI CAC'GTC
Cuts at: 0 663 984 1247 1296
Size: 663 321 263 49
Fragments arranged by size:
663 321 263 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 628 1296
Size: 513 115 668
Fragments arranged by size:
668 513 115
BsaI GGTCTCn'nnnn_
Cuts at: 0 1066 1296
Size: 1066 230
BsaHI Gr'CG_yC
Cuts at: 0 592 1268 1296
Size: 592 676 28
Fragments arranged by size:
676 592 28
BsaWI w'CCGG_w
Cuts at: 0 1241 1296
Size: 1241 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 511 541 670 700 1296
Size: 511 30 129 30 596
Fragments arranged by size:
596 511 129 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 1296
Size: 217 1079
BseYI C'CCAG_C
Cuts at: 0 164 252 260 395 405 575 623 726
Size: 164 88 8 135 10 170 48 103
Cuts at: 726 904 1038 1296
Size: 178 134 258
Fragments arranged by size:
258 178 170 164 135 134 103 88
48 10 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 532 736 760 1296
Size: 232 300 204 24 536
Fragments arranged by size:
536 300 232 204 24
BsiHKAI G_wGCw'C
Cuts at: 0 302 481 835 1296
Size: 302 179 354 461
Fragments arranged by size:
461 354 302 179
BsmI GAATG_Cn'
Cuts at: 0 248 1296
Size: 248 1048
Bsp1286I G_dGCh'C
Cuts at: 0 15 124 238 271 302 396 481 503
Size: 15 109 114 33 31 94 85 22
Cuts at: 503 749 835 962 1283 1296
Size: 246 86 127 321 13
Fragments arranged by size:
321 246 127 114 109 94 86 85
33 31 22 15 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 285 677 767 1296
Size: 285 392 90 529
Fragments arranged by size:
529 392 285 90
BsrBI CCG'CTC
Cuts at: 0 1035 1296
Size: 1035 261
BsrDI GCAATG_nn'
Cuts at: 0 959 1296
Size: 959 337
BsrFI r'CCGG_y
Cuts at: 0 226 561 1296
Size: 226 335 735
Fragments arranged by size:
735 335 226
BssSI C'ACGA_G
Cuts at: 0 289 1296
Size: 289 1007
BstAPI GCAn_nnn'nTGC
Cuts at: 0 492 759 861 1296
Size: 492 267 102 435
Fragments arranged by size:
492 435 267 102
BstEII G'GTnAC_C
Cuts at: 0 330 1296
Size: 330 966
BstXI CCAn_nnnn'nTGG
Cuts at: 0 588 972 1296
Size: 588 384 324
Fragments arranged by size:
588 384 324
BtgI C'CryG_G
Cuts at: 0 7 47 820 1296
Size: 7 40 773 476
Fragments arranged by size:
773 476 40 7
BtsI GCAGTG_nn'
Cuts at: 0 26 396 859 1296
Size: 26 370 463 437
Fragments arranged by size:
463 437 370 26
DraIII CAC_nnn'GTG
Cuts at: 0 1214 1296
Size: 1214 82
EaeI y'GGCC_r
Cuts at: 0 31 128 1028 1296
Size: 31 97 900 268
Fragments arranged by size:
900 268 97 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 30 1296
Size: 30 1266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 628 1134 1296
Size: 513 115 506 162
Fragments arranged by size:
513 506 162 115
EcoO109I rG'GnC_Cy
Cuts at: 0 73 120 121 307 958 1014 1296
Size: 73 47 1 186 651 56 282
Fragments arranged by size:
651 282 186 73 56 47 1
HaeII r_GCGC'y
Cuts at: 0 156 210 595 1296
Size: 156 54 385 701
Fragments arranged by size:
701 385 156 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 670 702 1296
Size: 670 32 594
Fragments arranged by size:
670 594 32
HincII GTy'rAC
Cuts at: 0 1221 1296
Size: 1221 75
KasI G'GCGC_C
Cuts at: 0 591 1296
Size: 591 705
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1103 1296
Size: 1103 193
MscI TGG'CCA
Cuts at: 0 33 130 1296
Size: 33 97 1166
Fragments arranged by size:
1166 97 33
MslI CAynn'nnrTG
Cuts at: 0 337 586 1296
Size: 337 249 710
Fragments arranged by size:
710 337 249
MspA1I CmG'CkG
Cuts at: 0 57 492 648 774 1173 1296
Size: 57 435 156 126 399 123
Fragments arranged by size:
435 399 156 126 123 57
NaeI GCC'GGC
Cuts at: 0 228 1296
Size: 228 1068
NarI GG'CG_CC
Cuts at: 0 592 1296
Size: 592 704
NcoI C'CATG_G
Cuts at: 0 7 1296
Size: 7 1289
NgoMIV G'CCGG_C
Cuts at: 0 226 1296
Size: 226 1070
NspI r_CATG'y
Cuts at: 0 413 1296
Size: 413 883
PasI CC'CwG_GG
Cuts at: 0 597 690 1283 1296
Size: 597 93 593 13
Fragments arranged by size:
597 593 93 13
PpuMI rG'GwC_Cy
Cuts at: 0 73 307 1296
Size: 73 234 989
Fragments arranged by size:
989 234 73
PspOMI G'GGCC_C
Cuts at: 0 120 958 1296
Size: 120 838 338
Fragments arranged by size:
838 338 120
PspXI vC'TCGA_Gb
Cuts at: 0 115 1296
Size: 115 1181
PstI C_TGCA'G
Cuts at: 0 391 620 854 1296
Size: 391 229 234 442
Fragments arranged by size:
442 391 234 229
PvuII CAG'CTG
Cuts at: 0 492 1173 1296
Size: 492 681 123
Fragments arranged by size:
681 492 123
SfcI C'TryA_G
Cuts at: 0 365 387 616 850 1296
Size: 365 22 229 234 446
Fragments arranged by size:
446 365 234 229 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 119 1296
Size: 119 1177
SfoI GGC'GCC
Cuts at: 0 593 1296
Size: 593 703
SmaI CCC'GGG
Cuts at: 0 89 145 1057 1296
Size: 89 56 912 239
Fragments arranged by size:
912 239 89 56
SmlI C'TyrA_G
Cuts at: 0 115 1296
Size: 115 1181
SphI G_CATG'C
Cuts at: 0 413 1296
Size: 413 883
StuI AGG'CCT
Cuts at: 0 511 1296
Size: 511 785
StyI C'CwwG_G
Cuts at: 0 7 348 1296
Size: 7 341 948
Fragments arranged by size:
948 341 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 266 274 598 850 1296
Size: 266 8 324 252 446
Fragments arranged by size:
446 324 266 252 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 511 543 1296
Size: 511 32 753
Fragments arranged by size:
753 511 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 379 969 1296
Size: 379 590 327
Fragments arranged by size:
590 379 327
XhoI C'TCGA_G
Cuts at: 0 115 1296
Size: 115 1181
XmaI C'CCGG_G
Cuts at: 0 87 143 1055 1296
Size: 87 56 912 241
Fragments arranged by size:
912 241 87 56
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BglI Bme1580I BmgBI BpmI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstEII BstXI BtgI BtsI DraIII EaeI EciI Eco57MI
EcoO109I HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PpuMI
PspOMI PspXI PstI PvuII SfcI SfiI SfoI SmaI
SmlI SphI StuI StyI TaqII TstI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsiEI
BsiWI BsmBI BspEI BspHI BsrGI BssHII BstBI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI TatI Tth111I
XbaI XmnI ZraI