(Circular) (Six-base) MAPSORT of: pFN21ASDA1098.seq Check: 8881 from: 1 to: 6208 pFN21ASDA1098 6208 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2167 2198 3007 2167 Size: 31 809 5368 Fragments arranged by size: 5368 809 31 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4018 278 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3551 1937 Size: 1614 4594 Acc65I G'GTAC_C Cuts at: 3529 3529 Size: 6208 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2917 4271 5655 5923 1632 Size: 1285 1354 1384 268 1917 Fragments arranged by size: 1917 1384 1354 1285 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 2040 3142 5107 6084 1203 Size: 837 1102 1965 977 1327 Fragments arranged by size: 1965 1327 1102 977 837 AgeI A'CCGG_T Cuts at: 5039 5039 Size: 6208 AhdI GACnn_n'nnGTC Cuts at: 2212 4937 2212 Size: 2725 3483 AleI CACnn'nnGTG Cuts at: 1166 3477 1166 Size: 2311 3897 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6208 AlwNI CAG_nnn'CTG Cuts at: 3951 5523 3951 Size: 1572 4636 ApaLI G'TGCA_C Cuts at: 3426 4265 5421 3426 Size: 839 1156 4213 Fragments arranged by size: 4213 1156 839 ApoI r'AATT_y Cuts at: 1493 3517 3743 3895 6004 1493 Size: 2024 226 152 2109 1697 Fragments arranged by size: 2109 2024 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 2048 2360 3157 3202 3533 1870 Size: 73 105 312 797 45 331 4545 Fragments arranged by size: 4545 797 331 312 105 73 45 BamHI G'GATC_C Cuts at: 2580 3538 3905 2580 Size: 958 367 4883 Fragments arranged by size: 4883 958 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2415 3046 3434 3529 4984 Size: 325 217 627 628 631 388 95 1455 Cuts at: 4984 618 Size: 1842 Fragments arranged by size: 1842 1455 631 628 627 388 325 217 95 BanII G_rGCy'C Cuts at: 1391 2879 3065 3086 3527 1391 Size: 1488 186 21 441 4072 Fragments arranged by size: 4072 1488 441 186 21 BbeI G_GCGC'C Cuts at: 3050 3438 3050 Size: 388 5820 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3064 928 Size: 971 1165 4072 Fragments arranged by size: 4072 1165 971 BbvCI CC'TCA_GC Cuts at: 3165 3165 Size: 6208 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2796 2830 2836 2870 4399 4433 1903 Size: 34 859 34 6 34 1529 34 3678 Fragments arranged by size: 3678 1529 859 34 34 34 34 6 BciVI GTATCCnnnnn_n' Cuts at: 4100 5310 4100 Size: 1210 4998 BclI T'GATC_A Cuts at: 1548 1548 Size: 6208 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2510 3060 4819 Size: 107 122 71 631 605 838 550 1759 Cuts at: 4819 136 Size: 1525 Fragments arranged by size: 1759 1525 838 631 605 550 122 107 71 BglII A'GATC_T Cuts at: 6203 6203 Size: 6208 BlpI GC'TnA_GC Cuts at: 2345 3625 2345 Size: 1280 4928 Bme1580I G_kGCm'C Cuts at: 1790 2418 3430 4269 5425 1790 Size: 628 1012 839 1156 2573 Fragments arranged by size: 2573 1156 1012 839 628 BmgBI CAC'GTC Cuts at: 1318 2041 3273 1318 Size: 723 1232 4253 Fragments arranged by size: 4253 1232 723 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2189 3939 4897 Size: 416 53 83 399 24 772 1750 958 Cuts at: 4897 442 Size: 1753 Fragments arranged by size: 1753 1750 958 772 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3190 3190 Size: 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3145 3338 3429 4868 1239 Size: 1906 193 91 1439 2579 Fragments arranged by size: 2579 1906 1439 193 91 Bpu10I CC'TnA_GC Cuts at: 3165 3165 Size: 6208 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2635 4333 5198 5496 2635 Size: 1698 865 298 3347 Fragments arranged by size: 3347 1698 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4871 882 Size: 617 3372 2219 Fragments arranged by size: 3372 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3143 3505 3999 493 Size: 711 1939 362 494 2702 Fragments arranged by size: 2702 1939 711 494 362 BsaBI GATnn'nnATC Cuts at: 6202 6202 Size: 6208 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3047 3435 4015 4397 Size: 53 83 186 936 1514 388 580 382 Cuts at: 4397 5032 275 Size: 635 1451 Fragments arranged by size: 1514 1451 936 635 580 388 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4641 5039 5313 5460 5871 4641 Size: 398 274 147 411 4978 Fragments arranged by size: 4978 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2363 2393 2861 2891 3305 Size: 30 165 30 960 30 468 30 414 Cuts at: 3305 3335 1178 Size: 30 4051 Fragments arranged by size: 4051 960 468 414 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2291 2639 2669 2723 3031 3341 1186 Size: 901 204 348 30 54 308 310 4053 Fragments arranged by size: 4053 901 348 310 308 204 54 30 BseYI C'CCAG_C Cuts at: 2166 3058 3265 5411 5907 2166 Size: 892 207 2146 496 2467 Fragments arranged by size: 2467 2146 892 496 207 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2195 2315 2444 2485 2800 3035 1163 Size: 573 459 120 129 41 315 235 4336 Fragments arranged by size: 4336 573 459 315 235 129 120 41 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3658 4419 5447 1540 Size: 275 186 1657 761 1028 2301 Fragments arranged by size: 2301 1657 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3430 4269 4354 5425 3430 Size: 839 85 1071 4213 Fragments arranged by size: 4213 1071 839 85 BsmI GAATG_Cn' Cuts at: 3719 3812 3719 Size: 93 6115 BsmBI CGTCTCn'nnnn_ Cuts at: 2705 2705 Size: 6208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2418 2879 3065 3086 3430 3527 4269 Size: 399 628 461 186 21 344 97 742 Cuts at: 4269 4354 5425 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 628 461 399 344 186 97 85 21 BspEI T'CCGG_A Cuts at: 5871 5871 Size: 6208 BspHI T'CATG_A Cuts at: 2781 4097 5827 2781 Size: 1316 1730 3162 Fragments arranged by size: 3162 1730 1316 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2167 2198 2235 3007 3563 844 Size: 1323 31 37 772 556 3489 Fragments arranged by size: 3489 1323 772 556 37 31 BsrBI CCG'CTC Cuts at: 1394 1823 2029 2061 2740 4095 1394 Size: 429 206 32 679 1355 3507 Fragments arranged by size: 3507 1355 679 429 206 32 BsrDI GCAATG_nn' Cuts at: 65 1248 4703 4877 65 Size: 1183 3455 174 1396 Fragments arranged by size: 3455 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2428 4852 5039 1700 Size: 110 618 2424 187 2869 Fragments arranged by size: 2869 2424 618 187 110 BsrGI T'GTAC_A Cuts at: 96 3372 96 Size: 3276 2932 BssHII G'CGCG_C Cuts at: 1925 2289 2291 1925 Size: 364 2 5842 Fragments arranged by size: 5842 364 2 BssSI C'ACGA_G Cuts at: 4268 5280 4268 Size: 1012 5196 BstAPI GCAn_nnn'nTGC Cuts at: 794 2422 2485 794 Size: 1628 63 4517 Fragments arranged by size: 4517 1628 63 BstBI TT'CG_AA Cuts at: 2007 5012 2007 Size: 3005 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6208 BstYI r'GATC_y Cuts at: 1972 2580 3538 3905 4290 4307 5759 5845 5857 Size: 608 958 367 385 17 1452 86 12 Cuts at: 5857 6203 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 958 608 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2146 2335 2646 2820 3001 513 Size: 552 957 124 189 311 174 181 3720 Fragments arranged by size: 3720 957 552 311 189 181 174 124 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2521 487 Size: 683 56 1295 4174 Fragments arranged by size: 4174 1295 683 56 BtsI GCAGTG_nn' Cuts at: 2439 2483 3247 3361 3724 4518 4538 2439 Size: 44 764 114 363 794 20 4109 Fragments arranged by size: 4109 794 764 363 114 44 20 ClaI AT'CG_AT Cuts at: 1554 2856 1554 Size: 1302 4906 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6173 DraI TTT'AAA Cuts at: 3512 3512 Size: 6208 DraIII CAC_nnn'GTG Cuts at: 2422 3253 2422 Size: 831 5377 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5215 809 Size: 507 3899 1802 Fragments arranged by size: 3899 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2131 2649 2679 Size: 54 1425 50 275 18 301 518 30 Cuts at: 2679 3288 3655 4544 6173 8 Size: 609 367 889 1629 43 Fragments arranged by size: 1629 1425 889 609 518 367 301 275 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 3655 1537 Size: 275 1843 4090 Fragments arranged by size: 4090 1843 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2357 2816 4138 1353 Size: 1004 459 1322 3423 Fragments arranged by size: 3423 1322 1004 459 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4795 5169 5315 387 Size: 1064 138 3206 374 146 1280 Fragments arranged by size: 3206 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2917 3145 3338 3429 4271 4868 5655 Size: 393 1285 228 193 91 842 597 787 Cuts at: 5655 5923 1239 Size: 268 1524 Fragments arranged by size: 1524 1285 842 787 597 393 268 228 193 91 EcoRI G'AATT_C Cuts at: 3517 3895 3517 Size: 378 5830 FspI TGC'GCA Cuts at: 2231 3458 4714 2231 Size: 1227 1256 3725 Fragments arranged by size: 3725 1256 1227 HaeII r_GCGC'y Cuts at: 1678 2524 3050 3165 3361 3438 5355 1678 Size: 846 526 115 196 77 1917 2531 Fragments arranged by size: 2531 1917 846 526 196 115 77 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2198 2230 3185 3217 4849 Size: 32 135 32 626 32 955 32 1632 Cuts at: 4849 4881 4923 4955 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1632 955 626 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3552 3798 669 Size: 988 281 1614 246 3079 Fragments arranged by size: 3079 1614 988 281 246 HindIII A'AGCT_T Cuts at: 748 2600 748 Size: 1852 4356 HpaI GTT'AAC Cuts at: 3798 3798 Size: 6208 KasI G'GCGC_C Cuts at: 3046 3434 3046 Size: 388 5820 KpnI G_GTAC'C Cuts at: 3533 3533 Size: 6208 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 6084 6084 Size: 6208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5322 5506 1305 Size: 4017 184 2007 Fragments arranged by size: 4017 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3290 10 Size: 54 1425 343 1458 2928 Fragments arranged by size: 2928 1458 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3477 4166 4525 4684 518 Size: 648 2311 689 359 159 2042 Fragments arranged by size: 2311 2042 689 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2148 2163 2337 2957 3183 3624 4301 5449 Size: 542 15 174 620 226 441 677 1148 Cuts at: 5449 5694 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 620 542 441 245 226 174 15 NarI GG'CG_CC Cuts at: 3047 3435 3047 Size: 388 5820 NcoI C'CATG_G Cuts at: 513 1065 2022 2646 2820 3001 513 Size: 552 957 624 174 181 3720 Fragments arranged by size: 3720 957 624 552 181 174 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NruI TCG'CGA Cuts at: 2607 2607 Size: 6208 NspI r_CATG'y Cuts at: 1132 1229 2483 2851 3566 5111 1132 Size: 97 1254 368 715 1545 2229 Fragments arranged by size: 2229 1545 1254 715 368 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6208 PciI A'CATG_T Cuts at: 5107 5107 Size: 6208 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6208 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4277 4309 5790 5822 4277 Size: 32 1481 32 4663 Fragments arranged by size: 4663 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6208 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PspXI vC'TCGA_Gb Cuts at: 2360 2360 Size: 6208 PstI C_TGCA'G Cuts at: 830 2748 2886 3120 3560 830 Size: 1918 138 234 440 3478 Fragments arranged by size: 3478 1918 440 234 138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 PvuII CAG'CTG Cuts at: 2957 3183 2957 Size: 226 5982 SacII CC_GC'GG Cuts at: 2149 2338 2149 Size: 189 6019 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SbfI CC_TGCA'GG Cuts at: 3560 3560 Size: 6208 ScaI AGT'ACT Cuts at: 4456 6168 4456 Size: 1712 4496 SexAI A'CCwGG_T Cuts at: 2253 2253 Size: 6208 SfcI C'TryA_G Cuts at: 826 1045 2744 2882 3116 3556 4691 5372 5563 Size: 219 1699 138 234 440 1135 681 191 Cuts at: 5563 826 Size: 1471 Fragments arranged by size: 1699 1471 1135 681 440 234 219 191 138 SfiI GGCCn_nnn'nGGCC Cuts at: 2510 2510 Size: 6208 SfoI GGC'GCC Cuts at: 3048 3436 3048 Size: 388 5820 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6208 SmaI CCC'GGG Cuts at: 1872 2050 3535 1872 Size: 178 1485 4545 Fragments arranged by size: 4545 1485 178 SmlI C'TyrA_G Cuts at: 820 1017 1943 2360 2614 4312 5213 5475 820 Size: 197 926 417 254 1698 901 262 1553 Fragments arranged by size: 1698 1553 926 901 417 262 254 197 SnaBI TAC'GTA Cuts at: 493 3505 3999 493 Size: 3012 494 2702 Fragments arranged by size: 3012 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 2483 2851 3566 1132 Size: 1351 368 715 3774 Fragments arranged by size: 3774 1351 715 368 SspI AAT'ATT Cuts at: 5 52 4132 5 Size: 47 4080 2081 Fragments arranged by size: 4080 2081 47 StuI AGG'CCT Cuts at: 1517 2504 1517 Size: 987 5221 StyI C'CwwG_G Cuts at: 513 1065 2022 2499 2646 2820 3001 3106 3647 Size: 552 957 477 147 174 181 105 541 Cuts at: 3647 513 Size: 3074 Fragments arranged by size: 3074 957 552 541 477 181 174 147 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4235 4252 4405 4590 5035 325 Size: 1255 2655 17 153 185 445 1498 Fragments arranged by size: 2655 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3372 4454 6166 Size: 274 80 33 51 1443 1395 1082 1712 Cuts at: 6166 96 Size: 138 Fragments arranged by size: 1712 1443 1395 1082 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2363 2395 3305 3337 6168 6200 2363 Size: 32 910 32 2831 32 2371 Fragments arranged by size: 2831 2371 910 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2114 1316 Size: 798 5410 XbaI T'CTAG_A Cuts at: 3544 3544 Size: 6208 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3277 3389 3277 Size: 112 6096 XhoI C'TCGA_G Cuts at: 1943 2360 1943 Size: 417 5791 XmaI C'CCGG_G Cuts at: 1870 2048 3533 1870 Size: 178 1485 4545 Fragments arranged by size: 4545 1485 178 XmnI GAAnn'nnTTC Cuts at: 4337 4337 Size: 6208 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4016 276 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacI SanDI SapI SrfI SwaI