(Circular) (Six-base) MAPSORT of: pFN21ASDA1064.seq Check: 4138 from: 1 to: 8638 pFN21ASDA1064 8638 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3476 3476 Size: 8638 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4508 6448 278 Size: 53 83 186 936 2972 1940 2468 Fragments arranged by size: 2972 2468 1940 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5981 1937 Size: 4044 4594 Acc65I G'GTAC_C Cuts at: 5959 5959 Size: 8638 AclI AA'CG_TT Cuts at: 1563 6765 7138 1563 Size: 5202 373 3063 Fragments arranged by size: 5202 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2351 3539 4505 4509 5903 6701 8085 8353 Size: 719 1188 966 4 1394 798 1384 268 Cuts at: 8353 1632 Size: 1917 Fragments arranged by size: 1917 1394 1384 1188 966 798 719 268 4 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8441 AflIII A'CryG_T Cuts at: 1203 3744 7537 8514 1203 Size: 2541 3793 977 1327 Fragments arranged by size: 3793 2541 1327 977 AgeI A'CCGG_T Cuts at: 7469 7469 Size: 8638 AhdI GACnn_n'nnGTC Cuts at: 4879 7367 4879 Size: 2488 6150 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8638 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3647 5082 1184 Size: 2463 1435 4740 Fragments arranged by size: 4740 2463 1435 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5573 5605 5573 Size: 32 8606 AlwNI CAG_nnn'CTG Cuts at: 3730 6381 7953 3730 Size: 2651 1572 4415 Fragments arranged by size: 4415 2651 1572 ApaI G_GGCC'C Cuts at: 3684 3863 3870 3989 4019 4266 4327 4757 4816 Size: 179 7 119 30 247 61 430 59 Cuts at: 4816 5082 3684 Size: 266 7240 Fragments arranged by size: 7240 430 266 247 179 119 61 59 30 7 ApaLI G'TGCA_C Cuts at: 5762 6695 7851 5762 Size: 933 1156 6549 Fragments arranged by size: 6549 1156 933 ApoI r'AATT_y Cuts at: 1493 5947 6173 6325 8434 1493 Size: 4454 226 152 2109 1697 Fragments arranged by size: 4454 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7192 160 Size: 872 6160 1606 Fragments arranged by size: 6160 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8638 AvaI C'yCGr_G Cuts at: 1870 1943 2036 3018 3677 4856 4964 5101 5235 Size: 73 93 982 659 1179 108 137 134 Cuts at: 5235 5298 5347 5473 5735 5756 5963 1870 Size: 63 49 126 262 21 207 4545 Fragments arranged by size: 4545 1179 982 659 262 207 137 134 126 108 93 73 63 49 21 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4429 4462 4429 Size: 33 8605 BamHI G'GATC_C Cuts at: 4338 5968 6335 4338 Size: 1630 367 6641 Fragments arranged by size: 6641 1630 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3488 4286 4860 5105 5132 Size: 325 217 627 1701 798 574 245 27 Cuts at: 5132 5326 5525 5648 5741 5959 7414 618 Size: 194 199 123 93 218 1455 1842 Fragments arranged by size: 1842 1701 1455 798 627 574 325 245 218 217 199 194 123 93 27 BanII G_rGCy'C Cuts at: 1391 3017 3453 3684 3863 3870 3989 4019 4149 Size: 1626 436 231 179 7 119 30 130 Cuts at: 4149 4242 4266 4327 4757 4816 5082 5564 5586 Size: 93 24 61 430 59 266 482 22 Cuts at: 5586 5822 5957 1391 Size: 236 135 4072 Fragments arranged by size: 4072 1626 482 436 430 266 236 231 179 135 130 119 93 61 59 30 24 22 7 BbeI G_GCGC'C Cuts at: 4290 5109 5136 5330 4290 Size: 819 27 194 7598 Fragments arranged by size: 7598 819 194 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3111 4493 928 Size: 971 1212 1382 5073 Fragments arranged by size: 5073 1382 1212 971 BbvCI CC'TCA_GC Cuts at: 4312 4613 5322 5544 4312 Size: 301 709 222 7406 Fragments arranged by size: 7406 709 301 222 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3199 3233 3276 3310 6829 6863 1903 Size: 34 1262 34 43 34 3519 34 3678 Fragments arranged by size: 3678 3519 1262 43 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2236 6530 7740 2236 Size: 4294 1210 3134 Fragments arranged by size: 4294 3134 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8638 BfrBI ATG'CAT Cuts at: 3907 3907 Size: 8638 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2135 3060 4023 Size: 107 122 71 631 605 463 925 963 Cuts at: 4023 4322 4752 5573 7249 136 Size: 299 430 821 1676 1525 Fragments arranged by size: 1676 1525 963 925 821 631 605 463 430 299 122 107 71 BglII A'GATC_T Cuts at: 8633 8633 Size: 8638 BlpI GC'TnA_GC Cuts at: 4538 5450 6055 4538 Size: 912 605 7121 Fragments arranged by size: 7121 912 605 Bme1580I G_kGCm'C Cuts at: 1790 3684 3863 3870 3989 4019 4182 4266 4327 Size: 1894 179 7 119 30 163 84 61 Cuts at: 4327 4757 4816 4863 5010 5019 5082 5530 5651 Size: 430 59 47 147 9 63 448 121 Cuts at: 5651 5746 5766 5781 6699 7855 1790 Size: 95 20 15 918 1156 2573 Fragments arranged by size: 2573 1894 1156 918 448 430 179 163 147 121 119 95 84 63 61 59 47 30 20 15 9 7 BmgBI CAC'GTC Cuts at: 1318 4918 1318 Size: 3600 5038 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2457 3393 5220 Size: 416 53 83 399 24 1040 936 1827 Cuts at: 5220 5423 6369 7327 442 Size: 203 946 958 1753 Fragments arranged by size: 1827 1753 1040 958 946 936 416 399 203 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8638 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5347 5347 Size: 8638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4071 4256 4553 7298 1239 Size: 2832 185 297 2745 2579 Fragments arranged by size: 2832 2745 2579 297 185 Bpu10I CC'TnA_GC Cuts at: 4224 4312 4613 4925 5322 5544 5577 5821 4224 Size: 88 301 312 397 222 33 244 7041 Fragments arranged by size: 7041 397 312 301 244 222 88 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4426 4513 5156 6763 7628 7926 4426 Size: 87 643 1607 865 298 5138 Fragments arranged by size: 5138 1607 865 643 298 87 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2228 2841 3237 7301 882 Size: 617 729 613 396 4064 2219 Fragments arranged by size: 4064 2219 729 617 613 396 BsaAI yAC'GTr Cuts at: 493 1204 5935 6429 493 Size: 711 4731 494 2702 Fragments arranged by size: 4731 2702 711 494 BsaBI GATnn'nnATC Cuts at: 8632 8632 Size: 8638 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2127 4287 4505 5106 Size: 53 83 186 936 594 2160 218 601 Cuts at: 5106 5133 5327 6445 6827 7462 275 Size: 27 194 1118 382 635 1451 Fragments arranged by size: 2160 1451 1118 936 635 601 594 382 218 194 186 83 53 27 BsaWI w'CCGG_w Cuts at: 2249 3150 4427 7071 7469 7743 7890 8301 2249 Size: 901 1277 2644 398 274 147 411 2586 Fragments arranged by size: 2644 2586 1277 901 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2924 2951 2954 2981 5573 Size: 30 165 30 1521 27 3 27 2592 Cuts at: 5573 5603 1178 Size: 30 4213 Fragments arranged by size: 4213 2592 1521 165 30 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2100 2141 2246 2324 2398 2660 2663 2859 Size: 914 41 105 78 74 262 3 196 Cuts at: 2859 2921 3084 3573 4373 4376 4379 4390 4808 Size: 62 163 489 800 3 3 11 418 Cuts at: 4808 4811 5185 5284 1186 Size: 3 374 99 4540 Fragments arranged by size: 4540 914 800 489 418 374 262 196 163 105 99 78 74 62 41 11 3 3 3 3 BseYI C'CCAG_C Cuts at: 3814 4552 4757 4934 5529 5751 5837 7841 8337 Size: 738 205 177 595 222 86 2004 496 Cuts at: 8337 3814 Size: 4115 Fragments arranged by size: 4115 2004 738 595 496 222 205 177 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3217 5042 1163 Size: 573 1481 1825 4759 Fragments arranged by size: 4759 1825 1481 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2734 4277 5202 5406 6088 6849 Size: 275 186 733 1543 925 204 682 761 Cuts at: 6849 7877 1540 Size: 1028 2301 Fragments arranged by size: 2301 1543 1028 925 761 733 682 275 204 186 BsiHKAI G_wGCw'C Cuts at: 3453 4215 4230 4956 5564 5766 6699 6784 7855 Size: 762 15 726 608 202 933 85 1071 Cuts at: 7855 3453 Size: 4236 Fragments arranged by size: 4236 1071 933 762 726 608 202 85 15 BsmI GAATG_Cn' Cuts at: 6149 6242 6149 Size: 93 8545 BsmBI CGTCTCn'nnnn_ Cuts at: 2913 3434 2913 Size: 521 8117 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3017 3453 3684 3863 3870 3989 4019 Size: 399 1227 436 231 179 7 119 30 Cuts at: 4019 4149 4182 4215 4230 4242 4266 4327 4757 Size: 130 33 33 15 12 24 61 430 Cuts at: 4757 4816 4863 4956 5010 5019 5082 5530 5564 Size: 59 47 93 54 9 63 448 34 Cuts at: 5564 5586 5651 5746 5766 5781 5822 5957 6699 Size: 22 65 95 20 15 41 135 742 Cuts at: 6699 6784 7855 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1227 1071 742 448 436 430 399 231 179 135 130 119 95 93 85 65 63 61 59 54 47 41 34 33 33 30 24 22 20 15 15 12 9 7 BspEI T'CCGG_A Cuts at: 2249 8301 2249 Size: 6052 2586 BspHI T'CATG_A Cuts at: 6527 8257 6527 Size: 1730 6908 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3476 5778 5993 844 Size: 2632 2302 215 3489 Fragments arranged by size: 3489 2632 2302 215 BsrBI CCG'CTC Cuts at: 1394 1823 2170 2353 2967 4810 5378 5454 6525 Size: 429 347 183 614 1843 568 76 1071 Cuts at: 6525 1394 Size: 3507 Fragments arranged by size: 3507 1843 1071 614 568 429 347 183 76 BsrDI GCAATG_nn' Cuts at: 65 1248 5557 7133 7307 65 Size: 1183 4309 1576 174 1396 Fragments arranged by size: 4309 1576 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2703 3700 4272 4289 4593 5189 5405 Size: 110 893 997 572 17 304 596 216 Cuts at: 5405 5509 5633 5679 7282 7469 1700 Size: 104 124 46 1603 187 2869 Fragments arranged by size: 2869 1603 997 893 596 572 304 216 187 124 110 104 46 17 BsrGI T'GTAC_A Cuts at: 96 3747 96 Size: 3651 4987 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8638 BssSI C'ACGA_G Cuts at: 6698 7710 6698 Size: 1012 7626 BstAPI GCAn_nnn'nTGC Cuts at: 794 3730 4867 5035 5770 794 Size: 2936 1137 168 735 3662 Fragments arranged by size: 3662 2936 1137 735 168 BstBI TT'CG_AA Cuts at: 2007 7442 2007 Size: 5435 3203 BstEII G'GTnAC_C Cuts at: 1184 2474 1184 Size: 1290 7348 BstXI CCAn_nnnn'nTGG Cuts at: 3383 4750 3383 Size: 1367 7271 BstYI r'GATC_y Cuts at: 1972 3759 4338 5968 6335 6720 6737 8189 8275 Size: 1787 579 1630 367 385 17 1452 86 Cuts at: 8275 8287 8633 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1787 1630 1452 579 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4334 4334 Size: 8638 BtgI C'CryG_G Cuts at: 513 1065 2022 2728 2779 2797 3004 3792 4011 Size: 552 957 706 51 18 207 788 219 Cuts at: 4011 4877 5019 5070 5112 5655 5910 513 Size: 866 142 51 42 543 255 3241 Fragments arranged by size: 3241 957 866 788 706 552 543 255 219 207 142 51 51 42 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2064 2436 2520 487 Size: 683 56 838 372 84 6605 Fragments arranged by size: 6605 838 683 372 84 56 BtsI GCAGTG_nn' Cuts at: 4474 4552 5010 5449 6154 6948 6968 4474 Size: 78 458 439 705 794 20 6144 Fragments arranged by size: 6144 794 705 458 439 78 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8638 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8603 DraI TTT'AAA Cuts at: 5896 5942 5896 Size: 46 8592 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7645 809 Size: 507 6329 1802 Fragments arranged by size: 6329 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 2731 3468 Size: 54 1425 50 275 18 306 595 737 Cuts at: 3468 4274 5199 5403 6085 6974 8603 8 Size: 806 925 204 682 889 1629 43 Fragments arranged by size: 1629 1425 925 889 806 737 682 595 306 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2731 4274 5199 5403 6085 1537 Size: 275 919 1543 925 204 682 4090 Fragments arranged by size: 4090 1543 925 919 682 275 204 EarI CTCTTCn'nnn_ Cuts at: 1353 2375 2859 2877 3432 4182 4223 5792 6568 Size: 1022 484 18 555 750 41 1569 776 Cuts at: 6568 1353 Size: 3423 Fragments arranged by size: 3423 1569 1022 776 750 555 484 41 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3102 5439 7225 7599 7745 387 Size: 1064 138 1513 2337 1786 374 146 1280 Fragments arranged by size: 2337 1786 1513 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3451 5562 3451 Size: 2111 6527 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2351 3539 4071 4256 4505 4509 4553 Size: 393 719 1188 532 185 249 4 44 Cuts at: 4553 5903 6701 7298 8085 8353 1239 Size: 1350 798 597 787 268 1524 Fragments arranged by size: 1524 1350 1188 798 787 719 597 532 393 268 249 185 44 4 EcoNI CCTnn'n_nnAGG Cuts at: 4571 4571 Size: 8638 EcoO109I rG'GnC_Cy Cuts at: 2040 2255 3681 3860 3878 3986 4016 4262 4263 Size: 215 1426 179 18 108 30 246 1 Cuts at: 4263 4323 4324 4558 4621 4679 4754 4813 4873 Size: 60 1 234 63 58 75 59 60 Cuts at: 4873 4969 5078 5079 5248 2040 Size: 96 109 1 169 5430 Fragments arranged by size: 5430 1426 246 234 215 179 169 109 108 96 75 63 60 60 59 58 30 18 1 1 1 EcoRI G'AATT_C Cuts at: 5947 6325 5947 Size: 378 8260 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4751 4954 4751 Size: 203 8435 FseI GG_CCGG'CC Cuts at: 4276 4276 Size: 8638 FspI TGC'GCA Cuts at: 7144 7144 Size: 8638 HaeII r_GCGC'y Cuts at: 1678 3232 4290 5101 5109 5136 5330 7785 1678 Size: 1554 1058 811 8 27 194 2455 2531 Fragments arranged by size: 2531 2455 1554 1058 811 194 27 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2300 2332 2414 2446 2924 Size: 32 135 32 728 32 82 32 478 Cuts at: 2924 2956 3701 3733 4235 4267 4865 4897 5861 Size: 32 745 32 502 32 598 32 964 Cuts at: 5861 5893 7279 7311 7353 7385 1373 Size: 32 1386 32 42 32 2626 Fragments arranged by size: 2626 1386 964 745 728 598 502 478 135 82 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5982 6228 669 Size: 988 281 4044 246 3079 Fragments arranged by size: 4044 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8638 HpaI GTT'AAC Cuts at: 6228 6228 Size: 8638 KasI G'GCGC_C Cuts at: 4286 5105 5132 5326 4286 Size: 819 27 194 7598 Fragments arranged by size: 7598 819 194 27 KpnI G_GTAC'C Cuts at: 5963 5963 Size: 8638 MfeI C'AATT_G Cuts at: 6237 6237 Size: 8638 MluI A'CGCG_T Cuts at: 8514 8514 Size: 8638 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2178 3165 3632 7752 7936 1305 Size: 873 987 467 4120 184 2007 Fragments arranged by size: 4120 2007 987 873 467 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6816 Fragments arranged by size: 6816 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2295 3701 3980 4139 6596 6955 7114 Size: 648 1129 1406 279 159 2457 359 159 Cuts at: 7114 518 Size: 2042 Fragments arranged by size: 2457 2042 1406 1129 648 359 279 159 159 MspA1I CmG'CkG Cuts at: 1606 2730 2799 3730 3802 4888 5021 5711 6054 Size: 1124 69 931 72 1086 133 690 343 Cuts at: 6054 6731 7879 8124 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1124 1086 931 690 677 343 245 133 72 69 NaeI GCC'GGC Cuts at: 4274 4595 5511 4274 Size: 321 916 7401 Fragments arranged by size: 7401 916 321 NarI GG'CG_CC Cuts at: 4287 5106 5133 5327 4287 Size: 819 27 194 7598 Fragments arranged by size: 7598 819 194 27 NcoI C'CATG_G Cuts at: 513 1065 2022 4011 513 Size: 552 957 1989 5140 Fragments arranged by size: 5140 1989 957 552 NdeI CA'TA_TG Cuts at: 387 2506 387 Size: 2119 6519 NgoMIV G'CCGG_C Cuts at: 4272 4593 5509 4272 Size: 321 916 7401 Fragments arranged by size: 7401 916 321 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8638 NotI GC'GGCC_GC Cuts at: 6085 6085 Size: 8638 NruI TCG'CGA Cuts at: 2791 2791 Size: 8638 NsiI A_TGCA'T Cuts at: 3909 3909 Size: 8638 NspI r_CATG'y Cuts at: 1132 1229 2543 2599 3415 3748 3919 3931 3943 Size: 97 1314 56 816 333 171 12 12 Cuts at: 3943 3955 3967 3979 5996 7541 1132 Size: 12 12 12 2017 1545 2229 Fragments arranged by size: 2229 2017 1545 1314 816 333 171 97 56 12 12 12 12 12 PasI CC'CwG_GG Cuts at: 1348 3848 3863 3884 1348 Size: 2500 15 21 6102 Fragments arranged by size: 6102 2500 21 15 PciI A'CATG_T Cuts at: 3744 7537 3744 Size: 3793 4845 PflMI CCAn_nnn'nTGG Cuts at: 1108 4067 5837 1108 Size: 2959 1770 3909 Fragments arranged by size: 3909 2959 1770 PmeI GTTT'AAAC Cuts at: 5942 5942 Size: 8638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5573 5605 6707 6739 8220 8252 5573 Size: 32 1102 32 1481 32 5959 Fragments arranged by size: 5959 1481 1102 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2040 3878 4558 4621 4679 4873 4969 2040 Size: 1838 680 63 58 194 96 5709 Fragments arranged by size: 5709 1838 680 194 96 63 58 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8638 PsiI TTA'TAA Cuts at: 6208 6208 Size: 8638 PspOMI G'GGCC_C Cuts at: 3680 3859 3866 3985 4015 4262 4323 4753 4812 Size: 179 7 119 30 247 61 430 59 Cuts at: 4812 5078 3680 Size: 266 7240 Fragments arranged by size: 7240 430 266 247 179 119 61 59 30 7 PstI C_TGCA'G Cuts at: 830 3735 4893 5271 5704 5990 830 Size: 2905 1158 378 433 286 3478 Fragments arranged by size: 3478 2905 1158 433 378 286 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8638 PvuII CAG'CTG Cuts at: 3730 3802 4888 3730 Size: 72 1086 7480 Fragments arranged by size: 7480 1086 72 SacI G_AGCT'C Cuts at: 3453 5564 3453 Size: 2111 6527 SacII CC_GC'GG Cuts at: 2731 2800 5022 2731 Size: 69 2222 6347 Fragments arranged by size: 6347 2222 69 SalI G'TCGA_C Cuts at: 1936 5980 1936 Size: 4044 4594 SanDI GG'GwC_CC Cuts at: 2040 3878 4558 4621 4679 4873 2040 Size: 1838 680 63 58 194 5805 Fragments arranged by size: 5805 1838 680 194 63 58 SapI GCTCTTCn'nnn_ Cuts at: 4182 4182 Size: 8638 SbfI CC_TGCA'GG Cuts at: 5990 5990 Size: 8638 ScaI AGT'ACT Cuts at: 2939 3215 6886 8598 2939 Size: 276 3671 1712 2979 Fragments arranged by size: 3671 2979 1712 276 SfcI C'TryA_G Cuts at: 826 1045 2761 3731 4889 5267 5700 5986 7121 Size: 219 1716 970 1158 378 433 286 1135 Cuts at: 7121 7802 7993 826 Size: 681 191 1471 Fragments arranged by size: 1716 1471 1158 1135 970 681 433 378 286 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 4023 4023 Size: 8638 SfoI GGC'GCC Cuts at: 4288 5107 5134 5328 4288 Size: 819 27 194 7598 Fragments arranged by size: 7598 819 194 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8638 SgrAI Cr'CCGG_yG Cuts at: 1700 4289 1700 Size: 2589 6049 SmaI CCC'GGG Cuts at: 1872 2038 3679 4858 5103 5237 5300 5758 5965 Size: 166 1641 1179 245 134 63 458 207 Cuts at: 5965 1872 Size: 4545 Fragments arranged by size: 4545 1641 1179 458 245 207 166 134 63 SmlI C'TyrA_G Cuts at: 820 1017 1943 4441 4492 5171 6742 7643 7905 Size: 197 926 2498 51 679 1571 901 262 Cuts at: 7905 820 Size: 1553 Fragments arranged by size: 2498 1571 1553 926 901 679 262 197 51 SnaBI TAC'GTA Cuts at: 493 5935 6429 493 Size: 5442 494 2702 Fragments arranged by size: 5442 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8638 SphI G_CATG'C Cuts at: 1132 2543 5996 1132 Size: 1411 3453 3774 Fragments arranged by size: 3774 3453 1411 SrfI GCCC'GGGC Cuts at: 3679 5103 3679 Size: 1424 7214 SspI AAT'ATT Cuts at: 5 52 6562 5 Size: 47 6510 2081 Fragments arranged by size: 6510 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8638 StyI C'CwwG_G Cuts at: 513 1065 2022 2712 2943 2970 4011 4767 5535 Size: 552 957 690 231 27 1041 756 768 Cuts at: 5535 5571 5691 6077 513 Size: 36 120 386 3074 Fragments arranged by size: 3074 1041 957 768 756 690 552 386 231 120 36 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3449 3806 4024 5824 6665 6682 6835 Size: 1255 1869 357 218 1800 841 17 153 Cuts at: 6835 7020 7465 325 Size: 185 445 1498 Fragments arranged by size: 1869 1800 1498 1255 841 445 357 218 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2484 2493 2937 Size: 274 80 33 51 1443 507 9 444 Cuts at: 2937 3213 3747 6884 8596 96 Size: 276 534 3137 1712 138 Fragments arranged by size: 3137 1712 1443 534 507 444 276 274 138 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5620 5652 8598 8630 5620 Size: 32 2946 32 5628 Fragments arranged by size: 5628 2946 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8638 XbaI T'CTAG_A Cuts at: 5974 5974 Size: 8638 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3799 5041 3799 Size: 1242 7396 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8638 XmaI C'CCGG_G Cuts at: 1870 2036 3677 4856 5101 5235 5298 5756 5963 Size: 166 1641 1179 245 134 63 458 207 Cuts at: 5963 1870 Size: 4545 Fragments arranged by size: 4545 1641 1179 458 245 207 166 134 63 XmnI GAAnn'nnTTC Cuts at: 6767 6767 Size: 8638 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4506 6446 276 Size: 53 83 186 936 2972 1940 2468 Fragments arranged by size: 2972 2468 1940 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BsiWI BstZ17I DraIII EcoRV FspAI PacI PfoI PmlI PspXI PsrI RsrII SexAI SwaI