(Circular) (Six-base) MAPSORT of: pFN21ASDA1027.seq Check: 4307 from: 1 to: 12352 pFN21ASDA1027 12352 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5387 8527 5387 Size: 3140 9212 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5450 10162 278 Size: 53 83 186 936 3914 4712 2468 Fragments arranged by size: 4712 3914 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2087 3272 7036 8910 9695 1937 Size: 150 1185 3764 1874 785 4594 Fragments arranged by size: 4594 3764 1874 1185 785 150 Acc65I G'GTAC_C Cuts at: 9673 9673 Size: 12352 AclI AA'CG_TT Cuts at: 1563 10479 10852 1563 Size: 8916 373 3063 Fragments arranged by size: 8916 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2060 2306 2828 3192 3242 3474 4100 5052 Size: 428 246 522 364 50 232 626 952 Cuts at: 5052 5839 7209 8102 8837 8904 9020 9374 9609 Size: 787 1370 893 735 67 116 354 235 Cuts at: 9609 10415 11799 12067 1632 Size: 806 1384 268 1917 Fragments arranged by size: 1917 1384 1370 952 893 806 787 735 626 522 428 364 354 268 246 235 232 116 67 50 AfeI AGC'GCT Cuts at: 4966 7972 4966 Size: 3006 9346 AflII C'TTAA_G Cuts at: 820 1017 2455 4591 8408 820 Size: 197 1438 2136 3817 4764 Fragments arranged by size: 4764 3817 2136 1438 197 AflIII A'CryG_T Cuts at: 1203 6180 11251 12228 1203 Size: 4977 5071 977 1327 Fragments arranged by size: 5071 4977 1327 977 AgeI A'CCGG_T Cuts at: 11183 11183 Size: 12352 AhdI GACnn_n'nnGTC Cuts at: 3292 11081 3292 Size: 7789 4563 AleI CACnn'nnGTG Cuts at: 1166 6512 1166 Size: 5346 7006 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5592 9333 1184 Size: 4408 3741 4203 Fragments arranged by size: 4408 4203 3741 AlwNI CAG_nnn'CTG Cuts at: 3931 4397 4690 4771 4873 5293 5338 5456 5608 Size: 466 293 81 102 420 45 118 152 Cuts at: 5608 5821 6050 7334 7789 8491 10095 11667 3931 Size: 213 229 1284 455 702 1604 1572 4616 Fragments arranged by size: 4616 1604 1572 1284 702 466 455 420 293 229 213 152 118 102 81 45 ApaI G_GGCC'C Cuts at: 2769 6880 7673 8458 2769 Size: 4111 793 785 6663 Fragments arranged by size: 6663 4111 793 785 ApaLI G'TGCA_C Cuts at: 6079 9093 10409 11565 6079 Size: 3014 1316 1156 6866 Fragments arranged by size: 6866 3014 1316 1156 ApoI r'AATT_y Cuts at: 1493 3406 9661 9887 10039 12148 1493 Size: 1913 6255 226 152 2109 1697 Fragments arranged by size: 6255 2109 1913 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 10906 160 Size: 872 9874 1606 Fragments arranged by size: 9874 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12352 AvaI C'yCGr_G Cuts at: 1870 1943 2640 4108 5235 5385 5802 5826 6873 Size: 73 697 1468 1127 150 417 24 1047 Cuts at: 6873 6894 6904 7182 8451 9006 9677 1870 Size: 21 10 278 1269 555 671 4545 Fragments arranged by size: 4545 1468 1269 1127 1047 697 671 555 417 278 150 73 24 21 10 BamHI G'GATC_C Cuts at: 2127 9682 10049 2127 Size: 7555 367 4430 Fragments arranged by size: 7555 4430 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4192 5105 6091 7826 7943 Size: 325 217 627 2405 913 986 1735 117 Cuts at: 7943 9152 9673 11128 618 Size: 1209 521 1455 1842 Fragments arranged by size: 2405 1842 1735 1455 1209 986 913 627 521 325 217 117 BanII G_rGCy'C Cuts at: 1391 2169 2769 3921 5321 5920 6414 6816 6880 Size: 778 600 1152 1400 599 494 402 64 Cuts at: 6880 7673 7872 8458 8731 8871 8949 9671 1391 Size: 793 199 586 273 140 78 722 4072 Fragments arranged by size: 4072 1400 1152 793 778 722 600 599 586 494 402 273 199 140 78 64 BbeI G_GCGC'C Cuts at: 7830 7830 Size: 12352 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2072 2321 3056 5946 8090 8547 928 Size: 971 173 249 735 2890 2144 457 4733 Fragments arranged by size: 4733 2890 2144 971 735 457 249 173 BbvCI CC'TCA_GC Cuts at: 4554 4704 4801 8235 4554 Size: 150 97 3434 8671 Fragments arranged by size: 8671 3434 150 97 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5783 5817 6323 6357 10543 10577 1903 Size: 34 3846 34 506 34 4186 34 3678 Fragments arranged by size: 4186 3846 3678 506 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 10244 11454 10244 Size: 1210 11142 BclI T'GATC_A Cuts at: 1548 1548 Size: 12352 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3500 3542 3608 Size: 107 122 71 631 605 1828 42 66 Cuts at: 3608 4286 4394 4544 4790 5141 5585 5634 5663 Size: 678 108 150 246 351 444 49 29 Cuts at: 5663 6314 7364 7718 8462 8972 10963 136 Size: 651 1050 354 744 510 1991 1525 Fragments arranged by size: 1991 1828 1525 1050 744 678 651 631 605 510 444 354 351 246 150 122 108 107 71 66 49 42 29 BglII A'GATC_T Cuts at: 2856 5209 7146 12347 2856 Size: 2353 1937 5201 2861 Fragments arranged by size: 5201 2861 2353 1937 BlpI GC'TnA_GC Cuts at: 3915 4827 4968 5320 9769 3915 Size: 912 141 352 4449 6498 Fragments arranged by size: 6498 4449 912 352 141 Bme1580I G_kGCm'C Cuts at: 1790 2374 2769 3003 3901 4153 5108 6083 6691 Size: 584 395 234 898 252 955 975 608 Cuts at: 6691 6880 7673 7885 8458 9097 10413 11569 1790 Size: 189 793 212 573 639 1316 1156 2573 Fragments arranged by size: 2573 1316 1156 975 955 898 793 639 608 584 573 395 252 234 212 189 BmgBI CAC'GTC Cuts at: 1318 7912 1318 Size: 6594 5758 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5348 5727 6155 Size: 416 53 83 399 24 3931 379 428 Cuts at: 6155 6685 7839 8161 10083 11041 442 Size: 530 1154 322 1922 958 1753 Fragments arranged by size: 3931 1922 1753 1154 958 530 428 416 399 379 322 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2927 1056 Size: 1871 10481 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3373 3670 7117 7921 3373 Size: 297 3447 804 7804 Fragments arranged by size: 7804 3447 804 297 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 3302 3408 3508 3836 4592 4793 6173 Size: 989 1074 106 100 328 756 201 1380 Cuts at: 6173 6740 6751 6972 7019 7430 7910 8480 11012 Size: 567 11 221 47 411 480 570 2532 Cuts at: 11012 1239 Size: 2579 Fragments arranged by size: 2579 2532 1380 1074 989 756 570 567 480 411 328 221 201 106 100 47 11 Bpu10I CC'TnA_GC Cuts at: 4554 4704 4801 5145 7280 8235 4554 Size: 150 97 344 2135 955 8671 Fragments arranged by size: 8671 2135 955 344 150 97 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2921 4058 5999 7089 10477 11342 11640 2921 Size: 1137 1941 1090 3388 865 298 3633 Fragments arranged by size: 3633 3388 1941 1137 1090 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2272 7909 8240 8292 11015 882 Size: 617 773 5637 331 52 2723 2219 Fragments arranged by size: 5637 2723 2219 773 617 331 52 BsaAI yAC'GTr Cuts at: 493 1204 2917 9649 10143 493 Size: 711 1713 6732 494 2702 Fragments arranged by size: 6732 2702 1713 711 494 BsaBI GATnn'nnATC Cuts at: 12346 12346 Size: 12352 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 4929 5447 7827 Size: 53 83 186 936 570 2826 518 2380 Cuts at: 7827 10159 10541 11176 275 Size: 2332 382 635 1451 Fragments arranged by size: 2826 2380 2332 1451 936 635 570 518 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5124 10785 11183 11457 11604 12015 5124 Size: 5661 398 274 147 411 5461 Fragments arranged by size: 5661 5461 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2696 2726 2936 2966 1178 Size: 30 165 30 1293 30 210 30 10564 Fragments arranged by size: 10564 1293 210 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2594 3281 3471 3754 4688 5537 6692 7427 Size: 1408 687 190 283 934 849 1155 735 Cuts at: 7427 7676 9062 1186 Size: 249 1386 4476 Fragments arranged by size: 4476 1408 1386 1155 934 849 735 687 283 249 190 BseYI C'CCAG_C Cuts at: 2140 2153 2213 3096 3435 3495 3944 4092 4397 Size: 13 60 883 339 60 449 148 305 Cuts at: 4397 4478 4866 4947 4961 5331 5762 6084 6229 Size: 81 388 81 14 370 431 322 145 Cuts at: 6229 7197 7201 7484 7666 7789 7931 8135 8212 Size: 968 4 283 182 123 142 204 77 Cuts at: 8212 9330 11555 12051 2140 Size: 1118 2225 496 2441 Fragments arranged by size: 2441 2225 1118 968 883 496 449 431 388 370 339 322 305 283 204 182 148 145 142 123 81 81 77 60 60 14 13 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2693 3560 3768 4784 4901 4943 5163 Size: 573 957 867 208 1016 117 42 220 Cuts at: 5163 5424 5483 5813 6327 6516 7442 7858 8238 Size: 261 59 330 514 189 926 416 380 Cuts at: 8238 8717 1163 Size: 479 4798 Fragments arranged by size: 4798 1016 957 926 867 573 514 479 416 380 330 261 220 208 189 117 59 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 9802 10563 11591 1540 Size: 275 186 7801 761 1028 2301 Fragments arranged by size: 7801 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2586 5321 6083 6498 7872 8871 8949 9097 9645 Size: 2735 762 415 1374 999 78 148 548 Cuts at: 9645 10413 10498 11569 2586 Size: 768 85 1071 3369 Fragments arranged by size: 3369 2735 1374 1071 999 768 762 548 415 148 85 78 BsmI GAATG_Cn' Cuts at: 4747 6771 9863 9956 4747 Size: 2024 3092 93 7143 Fragments arranged by size: 7143 3092 2024 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2582 3111 6128 2582 Size: 529 3017 8806 Fragments arranged by size: 8806 3017 529 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2374 2586 2769 3003 3901 3921 Size: 399 379 205 212 183 234 898 20 Cuts at: 3921 4153 5108 5321 5920 6083 6414 6498 6691 Size: 232 955 213 599 163 331 84 193 Cuts at: 6691 6816 6880 7673 7872 7885 8458 8731 8871 Size: 125 64 793 199 13 573 273 140 Cuts at: 8871 8949 9097 9645 9671 10413 10498 11569 1391 Size: 78 148 548 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 955 898 793 742 599 573 548 399 379 331 273 234 232 213 212 205 199 193 183 163 148 140 125 85 84 78 64 26 20 13 BspEI T'CCGG_A Cuts at: 5124 12015 5124 Size: 6891 5461 BspHI T'CATG_A Cuts at: 2358 2392 10241 11971 2358 Size: 34 7849 1730 2739 Fragments arranged by size: 7849 2739 1730 34 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3939 5363 5387 6285 8527 9301 9707 844 Size: 3095 1424 24 898 2242 774 406 3489 Fragments arranged by size: 3489 3095 2242 1424 898 774 406 24 BsrBI CCG'CTC Cuts at: 1394 1823 2125 7243 7808 10239 1394 Size: 429 302 5118 565 2431 3507 Fragments arranged by size: 5118 3507 2431 565 429 302 BsrDI GCAATG_nn' Cuts at: 65 1248 2471 3195 4980 5365 7014 7611 7986 Size: 1183 1223 724 1785 385 1649 597 375 Cuts at: 7986 8402 8625 8931 9075 9384 9513 10847 11021 Size: 416 223 306 144 309 129 1334 174 Cuts at: 11021 65 Size: 1396 Fragments arranged by size: 1785 1649 1396 1334 1223 1183 724 597 416 385 375 309 306 223 174 144 129 BsrFI r'CCGG_y Cuts at: 1700 1810 3200 4859 5742 6923 10996 11183 1700 Size: 110 1390 1659 883 1181 4073 187 2869 Fragments arranged by size: 4073 2869 1659 1390 1181 883 187 110 BsrGI T'GTAC_A Cuts at: 96 2650 6598 9265 96 Size: 2554 3948 2667 3183 Fragments arranged by size: 3948 3183 2667 2554 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12352 BssSI C'ACGA_G Cuts at: 2585 2679 10412 11424 2585 Size: 94 7733 1012 3513 Fragments arranged by size: 7733 3513 1012 94 BstAPI GCAn_nnn'nTGC Cuts at: 794 2515 3538 4055 4312 4690 5378 6350 7262 Size: 1721 1023 517 257 378 688 972 912 Cuts at: 7262 7334 7478 9257 794 Size: 72 144 1779 3889 Fragments arranged by size: 3889 1779 1721 1023 972 912 688 517 378 257 144 72 BstBI TT'CG_AA Cuts at: 2007 11156 2007 Size: 9149 3203 BstEII G'GTnAC_C Cuts at: 1184 7373 7636 7813 8386 1184 Size: 6189 263 177 573 5150 Fragments arranged by size: 6189 5150 573 263 177 BstXI CCAn_nnnn'nTGG Cuts at: 3010 3965 4045 4087 4261 5101 5788 6514 6616 Size: 955 80 42 174 840 687 726 102 Cuts at: 6616 8119 8293 8383 9097 9172 3010 Size: 1503 174 90 714 75 6190 Fragments arranged by size: 6190 1503 955 840 726 714 687 174 174 102 90 80 75 42 BstYI r'GATC_y Cuts at: 1972 2127 2289 2754 2856 3648 4605 5209 5452 Size: 155 162 465 102 792 957 604 243 Cuts at: 5452 7146 9594 9682 10049 10434 10451 11903 11989 Size: 1694 2448 88 367 385 17 1452 86 Cuts at: 11989 12001 12347 1972 Size: 12 346 1977 Fragments arranged by size: 2448 1977 1694 1452 957 792 604 465 385 367 346 243 162 155 102 88 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5660 5814 5833 6792 5660 Size: 154 19 959 11220 Fragments arranged by size: 11220 959 154 19 BtgI C'CryG_G Cuts at: 513 1065 2022 2091 2548 3787 4260 4467 5991 Size: 552 957 69 457 1239 473 207 1524 Cuts at: 5991 6807 7795 8175 8424 8571 513 Size: 816 988 380 249 147 4294 Fragments arranged by size: 4294 1524 1239 988 957 816 552 473 457 380 249 207 147 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4801 5311 5658 9511 487 Size: 683 56 3575 510 347 3853 3328 Fragments arranged by size: 3853 3575 3328 683 510 347 56 BtsI GCAGTG_nn' Cuts at: 3744 3838 3882 3901 4902 4913 5466 5601 7131 Size: 94 44 19 1001 11 553 135 1530 Cuts at: 7131 7263 7353 7623 7841 8019 8277 9191 9414 Size: 132 90 270 218 178 258 914 223 Cuts at: 9414 9868 10662 10682 3744 Size: 454 794 20 5414 Fragments arranged by size: 5414 1530 1001 914 794 553 454 270 258 223 218 178 135 132 94 90 44 20 19 11 ClaI AT'CG_AT Cuts at: 1554 3210 1554 Size: 1656 10696 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2860 2895 6403 6438 8829 8864 8914 Size: 35 2296 35 3508 35 2391 35 50 Cuts at: 8914 8949 529 Size: 35 3932 Fragments arranged by size: 3932 3508 2391 2296 50 35 35 35 35 35 DraI TTT'AAA Cuts at: 9656 9656 Size: 12352 DraIII CAC_nnn'GTG Cuts at: 4148 5186 6078 6323 7213 4148 Size: 1038 892 245 890 9287 Fragments arranged by size: 9287 1038 892 890 245 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3781 8114 8927 11359 809 Size: 507 2465 4333 813 2432 1802 Fragments arranged by size: 4333 2465 2432 1802 813 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3454 3589 4408 Size: 54 1425 50 275 18 1624 135 819 Cuts at: 4408 4719 5484 5542 5635 6097 6618 6921 6925 Size: 311 765 58 93 462 521 303 4 Cuts at: 6925 7011 7170 7702 8740 9228 9799 10688 12317 Size: 86 159 532 1038 488 571 889 1629 Cuts at: 12317 8 Size: 43 Fragments arranged by size: 1629 1624 1425 1038 889 819 765 571 532 521 488 462 311 303 275 159 135 93 86 58 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 9799 1537 Size: 275 7987 4090 Fragments arranged by size: 7987 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2421 7746 9459 9564 10282 1353 Size: 1068 5325 1713 105 718 3423 Fragments arranged by size: 5325 3423 1713 1068 718 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 10939 11313 11459 387 Size: 1064 138 9350 374 146 1280 Fragments arranged by size: 9350 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5319 7870 8869 8947 5319 Size: 2551 999 78 8724 Fragments arranged by size: 8724 2551 999 78 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2228 2306 2828 3192 3242 3302 Size: 393 428 168 78 522 364 50 60 Cuts at: 3302 3408 3474 3508 3836 4100 4592 4793 5052 Size: 106 66 34 328 264 492 201 259 Cuts at: 5052 5839 6173 6740 6751 6972 7019 7209 7430 Size: 787 334 567 11 221 47 190 221 Cuts at: 7430 7910 8102 8480 8837 8904 9020 9374 9609 Size: 480 192 378 357 67 116 354 235 Cuts at: 9609 10415 11012 11799 12067 1239 Size: 806 597 787 268 1524 Fragments arranged by size: 1524 806 787 787 597 567 522 492 480 428 393 378 364 357 354 334 328 268 264 259 235 221 221 201 192 190 168 116 106 78 67 66 60 50 47 34 11 EcoNI CCTnn'n_nnAGG Cuts at: 2822 2966 3848 5519 7049 7412 7466 2822 Size: 144 882 1671 1530 363 54 7708 Fragments arranged by size: 7708 1671 1530 882 363 144 54 EcoO109I rG'GnC_Cy Cuts at: 2137 2451 2644 2766 3712 3862 4351 5290 5554 Size: 314 193 122 946 150 489 939 264 Cuts at: 5554 5818 5983 6666 6877 6898 7042 7447 7670 Size: 264 165 683 211 21 144 405 223 Cuts at: 7670 8116 8239 8455 8920 9196 2137 Size: 446 123 216 465 276 5293 Fragments arranged by size: 5293 946 939 683 489 465 446 405 314 276 264 264 223 216 211 193 165 150 144 123 122 21 EcoRI G'AATT_C Cuts at: 9661 10039 9661 Size: 378 11974 EcoRV GAT'ATC Cuts at: 3214 3214 Size: 12352 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2812 3262 9563 2812 Size: 450 6301 5601 Fragments arranged by size: 6301 5601 450 FseI GG_CCGG'CC Cuts at: 6927 6927 Size: 12352 FspI TGC'GCA Cuts at: 3893 4715 4737 10858 3893 Size: 822 22 6121 5387 Fragments arranged by size: 6121 5387 822 22 HaeII r_GCGC'y Cuts at: 1678 4771 4968 6592 6659 7830 7974 11499 1678 Size: 3093 197 1624 67 1171 144 3525 2531 Fragments arranged by size: 3525 3093 2531 1624 1171 197 144 67 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2673 2705 3254 3278 3286 Size: 32 135 32 1101 32 549 24 8 Cuts at: 3286 3310 3587 3619 3640 3672 5437 5469 5590 Size: 24 277 32 21 32 1765 32 121 Cuts at: 5590 5622 7138 7170 8289 8321 9353 9385 10993 Size: 32 1516 32 1119 32 1032 32 1608 Cuts at: 10993 11025 11067 11099 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1765 1608 1516 1119 1101 1032 549 277 135 121 42 32 32 32 32 32 32 32 32 32 32 32 32 24 24 21 8 HincII GTy'rAC Cuts at: 669 1657 1938 3309 8829 9696 9942 669 Size: 988 281 1371 5520 867 246 3079 Fragments arranged by size: 5520 3079 1371 988 867 281 246 HindIII A'AGCT_T Cuts at: 748 2220 3115 5246 748 Size: 1472 895 2131 7854 Fragments arranged by size: 7854 2131 1472 895 HpaI GTT'AAC Cuts at: 9942 9942 Size: 12352 KasI G'GCGC_C Cuts at: 7826 7826 Size: 12352 KpnI G_GTAC'C Cuts at: 9677 9677 Size: 12352 MfeI C'AATT_G Cuts at: 9951 9951 Size: 12352 MluI A'CGCG_T Cuts at: 12228 12228 Size: 12352 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2534 3792 5650 5955 6777 8317 11466 11650 Size: 1229 1258 1858 305 822 1540 3149 184 Cuts at: 11650 1305 Size: 2007 Fragments arranged by size: 3149 2007 1858 1540 1258 1229 822 305 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3591 4721 5544 5637 6620 Size: 54 1425 343 1759 1130 823 93 983 Cuts at: 6620 7172 10 Size: 552 5190 Fragments arranged by size: 5190 1759 1425 1130 983 823 552 343 93 54 MslI CAynn'nnrTG Cuts at: 518 1166 2351 2357 2523 5948 6074 6512 7124 Size: 648 1185 6 166 3425 126 438 612 Cuts at: 7124 7616 7800 8009 8552 10310 10669 10828 518 Size: 492 184 209 543 1758 359 159 2042 Fragments arranged by size: 3425 2042 1758 1185 648 612 543 492 438 359 209 184 166 159 126 6 MspA1I CmG'CkG Cuts at: 1606 2144 2656 3453 3677 3944 4061 4096 4340 Size: 538 512 797 224 267 117 35 244 Cuts at: 4340 4655 4687 4703 4733 5030 5080 5540 5572 Size: 315 32 16 30 297 50 460 32 Cuts at: 5572 5616 5625 6025 6035 6341 6546 6997 7168 Size: 44 9 400 10 306 205 451 171 Cuts at: 7168 7201 7687 7733 8135 8327 8998 9263 9768 Size: 33 486 46 402 192 671 265 505 Cuts at: 9768 10445 11593 11838 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 797 677 671 538 512 505 486 460 451 402 400 315 306 297 267 265 245 244 224 205 192 171 117 50 46 44 35 33 32 32 30 16 10 9 NaeI GCC'GGC Cuts at: 3202 4861 6925 3202 Size: 1659 2064 8629 Fragments arranged by size: 8629 2064 1659 NarI GG'CG_CC Cuts at: 7827 7827 Size: 12352 NcoI C'CATG_G Cuts at: 513 1065 2022 2091 2548 4467 6807 7795 8571 Size: 552 957 69 457 1919 2340 988 776 Cuts at: 8571 513 Size: 4294 Fragments arranged by size: 4294 2340 1919 988 957 776 552 457 69 NdeI CA'TA_TG Cuts at: 387 387 Size: 12352 NgoMIV G'CCGG_C Cuts at: 3200 4859 6923 3200 Size: 1659 2064 8629 Fragments arranged by size: 8629 2064 1659 NheI G'CTAG_C Cuts at: 1052 2923 1052 Size: 1871 10481 NotI GC'GGCC_GC Cuts at: 9799 9799 Size: 12352 NspI r_CATG'y Cuts at: 1132 1229 2239 2356 3427 3478 3532 4376 4781 Size: 97 1010 117 1071 51 54 844 405 Cuts at: 4781 5157 6184 9710 11255 1132 Size: 376 1027 3526 1545 2229 Fragments arranged by size: 3526 2229 1545 1071 1027 1010 844 405 376 117 97 54 51 PasI CC'CwG_GG Cuts at: 1348 5353 8293 9193 1348 Size: 4005 2940 900 4507 Fragments arranged by size: 4507 4005 2940 900 PciI A'CATG_T Cuts at: 6180 11251 6180 Size: 5071 7281 PflMI CCAn_nnn'nTGG Cuts at: 1108 2632 4171 5293 5338 6287 6463 7915 1108 Size: 1524 1539 1122 45 949 176 1452 5545 Fragments arranged by size: 5545 1539 1524 1452 1122 949 176 45 PfoI T'CCnGG_A Cuts at: 3066 4014 5865 6141 6150 7305 3066 Size: 948 1851 276 9 1155 8113 Fragments arranged by size: 8113 1851 1155 948 276 9 PmeI GTTT'AAAC Cuts at: 9656 9656 Size: 12352 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 10421 10453 11934 11966 10421 Size: 32 1481 32 10807 Fragments arranged by size: 10807 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2451 2644 3712 5290 5554 5818 6898 7042 7447 Size: 193 1068 1578 264 264 1080 144 405 Cuts at: 7447 8116 8239 8920 2451 Size: 669 123 681 5883 Fragments arranged by size: 5883 1578 1080 1068 681 669 405 264 264 193 144 123 PshAI GACnn'nnGTC Cuts at: 1613 2550 1613 Size: 937 11415 PsiI TTA'TAA Cuts at: 9922 9922 Size: 12352 PspOMI G'GGCC_C Cuts at: 2765 6876 7669 8454 2765 Size: 4111 793 785 6663 Fragments arranged by size: 6663 4111 793 785 PspXI vC'TCGA_Gb Cuts at: 5826 5826 Size: 12352 PstI C_TGCA'G Cuts at: 830 3321 4060 4138 4371 4798 4825 5007 5461 Size: 2491 739 78 233 427 27 182 454 Cuts at: 5461 6034 6505 7069 7138 7258 7344 7732 7836 Size: 573 471 564 69 120 86 388 104 Cuts at: 7836 8326 8799 8962 8980 9457 9704 830 Size: 490 473 163 18 477 247 3478 Fragments arranged by size: 3478 2491 739 573 564 490 477 473 471 454 427 388 247 233 182 163 120 104 86 78 69 27 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12352 PvuII CAG'CTG Cuts at: 2144 2656 3677 3944 4061 4340 4655 4703 4733 Size: 512 1021 267 117 279 315 48 30 Cuts at: 4733 5030 5080 5540 5625 6025 6035 6341 6546 Size: 297 50 460 85 400 10 306 205 Cuts at: 6546 6997 7201 7687 8135 8327 8998 9263 2144 Size: 451 204 486 448 192 671 265 5233 Fragments arranged by size: 5233 1021 671 512 486 460 451 448 400 315 306 297 279 267 265 205 204 192 117 85 50 48 30 10 RsrII CG'GwC_CG Cuts at: 6139 6139 Size: 12352 SacI G_AGCT'C Cuts at: 5321 7872 8871 8949 5321 Size: 2551 999 78 8724 Fragments arranged by size: 8724 2551 999 78 SalI G'TCGA_C Cuts at: 1936 9694 1936 Size: 7758 4594 SanDI GG'GwC_CC Cuts at: 2644 3712 5554 6898 2644 Size: 1068 1842 1344 8098 Fragments arranged by size: 8098 1842 1344 1068 SapI GCTCTTCn'nnn_ Cuts at: 9459 9459 Size: 12352 SbfI CC_TGCA'GG Cuts at: 9704 9704 Size: 12352 ScaI AGT'ACT Cuts at: 7232 10600 12312 7232 Size: 3368 1712 7272 Fragments arranged by size: 7272 3368 1712 SexAI A'CCwGG_T Cuts at: 4527 4527 Size: 12352 SfcI C'TryA_G Cuts at: 826 1045 3317 4056 4134 4293 4367 4794 4821 Size: 219 2272 739 78 159 74 427 27 Cuts at: 4821 5003 5213 5457 6030 6501 7065 7134 7254 Size: 182 210 244 573 471 564 69 120 Cuts at: 7254 7340 7728 7832 8322 8795 8958 8976 9453 Size: 86 388 104 490 473 163 18 477 Cuts at: 9453 9700 10835 11516 11707 826 Size: 247 1135 681 191 1471 Fragments arranged by size: 2272 1471 1135 739 681 573 564 490 477 473 471 427 388 247 244 219 210 191 182 163 159 120 104 86 78 74 69 27 18 SfiI GGCCn_nnn'nGGCC Cuts at: 7718 7718 Size: 12352 SfoI GGC'GCC Cuts at: 7828 7828 Size: 12352 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12352 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12352 SmaI CCC'GGG Cuts at: 1872 2642 6875 6896 7184 9008 9679 1872 Size: 770 4233 21 288 1824 671 4545 Fragments arranged by size: 4545 4233 1824 770 671 288 21 SmlI C'TyrA_G Cuts at: 820 1017 1943 2455 2936 4073 4591 5235 5826 Size: 197 926 512 481 1137 518 644 591 Cuts at: 5826 6014 7104 8408 10456 11357 11619 820 Size: 188 1090 1304 2048 901 262 1553 Fragments arranged by size: 2048 1553 1304 1137 1090 926 901 644 591 518 512 481 262 197 188 SnaBI TAC'GTA Cuts at: 493 9649 10143 493 Size: 9156 494 2702 Fragments arranged by size: 9156 2702 494 SpeI A'CTAG_T Cuts at: 152 4168 9130 152 Size: 4016 4962 3374 Fragments arranged by size: 4962 4016 3374 SphI G_CATG'C Cuts at: 1132 3427 3532 4781 9710 1132 Size: 2295 105 1249 4929 3774 Fragments arranged by size: 4929 3774 2295 1249 105 SrfI GCCC'GGGC Cuts at: 6875 7184 6875 Size: 309 12043 SspI AAT'ATT Cuts at: 5 52 2402 10276 5 Size: 47 2350 7874 2081 Fragments arranged by size: 7874 2350 2081 47 StuI AGG'CCT Cuts at: 1517 2726 3611 4853 5039 5798 7112 7952 1517 Size: 1209 885 1242 186 759 1314 840 5917 Fragments arranged by size: 5917 1314 1242 1209 885 840 759 186 StyI C'CwwG_G Cuts at: 513 1065 2022 2091 2548 2823 2904 3021 3054 Size: 552 957 69 457 275 81 117 33 Cuts at: 3054 4467 4644 4848 5979 6609 6807 7359 7605 Size: 1413 177 204 1131 630 198 552 246 Cuts at: 7605 7795 7818 8112 8376 8559 8571 8583 9018 Size: 190 23 294 264 183 12 12 435 Cuts at: 9018 9139 9791 513 Size: 121 652 3074 Fragments arranged by size: 3074 1413 1131 957 652 630 552 552 457 435 294 275 264 246 204 198 190 183 177 121 117 81 69 33 23 12 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4361 4460 5928 6098 7250 7412 7448 Size: 1255 2781 99 1468 170 1152 162 36 Cuts at: 7448 7484 7532 7541 7961 8204 8712 9140 10379 Size: 36 48 9 420 243 508 428 1239 Cuts at: 10379 10396 10549 10734 11179 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2781 1498 1468 1255 1239 1152 508 445 428 420 243 185 170 162 153 99 48 36 36 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2262 2650 3160 Size: 274 80 33 51 1443 285 388 510 Cuts at: 3160 4178 5473 6598 7230 8782 9265 9609 10598 Size: 1018 1295 1125 632 1552 483 344 989 Cuts at: 10598 12310 96 Size: 1712 138 Fragments arranged by size: 1712 1552 1443 1295 1125 1018 989 632 510 483 388 344 285 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2696 2728 7197 7199 7229 7231 9136 9168 12312 Size: 32 4469 2 30 2 1905 32 3144 Cuts at: 12312 12344 2696 Size: 32 2704 Fragments arranged by size: 4469 3144 2704 1905 32 32 32 30 2 2 Tth111I GACn'n_nGTC Cuts at: 1316 4529 1316 Size: 3213 9139 XbaI T'CTAG_A Cuts at: 5200 9688 5200 Size: 4488 7864 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2759 4525 4627 5494 8761 9094 2759 Size: 1766 102 867 3267 333 6017 Fragments arranged by size: 6017 3267 1766 867 333 102 XhoI C'TCGA_G Cuts at: 1943 5235 5826 1943 Size: 3292 591 8469 Fragments arranged by size: 8469 3292 591 XmaI C'CCGG_G Cuts at: 1870 2640 6873 6894 7182 9006 9677 1870 Size: 770 4233 21 288 1824 671 4545 Fragments arranged by size: 4545 4233 1824 770 671 288 21 XmnI GAAnn'nnTTC Cuts at: 2813 9546 10481 2813 Size: 6733 935 4684 Fragments arranged by size: 6733 4684 935 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5448 10160 276 Size: 53 83 186 936 3914 4712 2468 Fragments arranged by size: 4712 3914 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I FspAI NruI NsiI PacI PmlI PsrI SacII SwaI