(Circular) (Six-base) MAPSORT of: pFN21ASDA0990.seq Check: 3385 from: 1 to: 7135 pFN21ASDA0990 7135 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3918 4945 278 Size: 53 83 186 936 2382 1027 2468 Fragments arranged by size: 2468 2382 1027 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3918 4478 1937 Size: 1981 560 4594 Fragments arranged by size: 4594 1981 560 Acc65I G'GTAC_C Cuts at: 4456 4456 Size: 7135 AclI AA'CG_TT Cuts at: 1563 5262 5635 1563 Size: 3699 373 3063 Fragments arranged by size: 3699 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2144 3575 3929 3985 5198 6582 6850 1632 Size: 512 1431 354 56 1213 1384 268 1917 Fragments arranged by size: 1917 1431 1384 1213 512 354 268 56 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6938 AflIII A'CryG_T Cuts at: 1203 2541 3432 3657 3692 4109 6034 7011 1203 Size: 1338 891 225 35 417 1925 977 1327 Fragments arranged by size: 1925 1338 1327 977 891 417 225 35 AgeI A'CCGG_T Cuts at: 5966 5966 Size: 7135 AhdI GACnn_n'nnGTC Cuts at: 5864 5864 Size: 7135 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7135 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7135 AlwNI CAG_nnn'CTG Cuts at: 4351 4878 6450 4351 Size: 527 1572 5036 Fragments arranged by size: 5036 1572 527 ApaI G_GGCC'C Cuts at: 2138 2138 Size: 7135 ApaLI G'TGCA_C Cuts at: 5192 6348 5192 Size: 1156 5979 ApoI r'AATT_y Cuts at: 1493 2910 3066 3938 4444 4670 4822 6931 1493 Size: 1417 156 872 506 226 152 2109 1697 Fragments arranged by size: 2109 1697 1417 872 506 226 156 152 AscI GG'CGCG_CC Cuts at: 2041 2041 Size: 7135 AseI AT'TA_AT Cuts at: 160 1032 5689 160 Size: 872 4657 1606 Fragments arranged by size: 4657 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7135 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2109 2156 2322 4460 1870 Size: 73 120 46 47 166 2138 4545 Fragments arranged by size: 4545 2138 166 120 73 47 46 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2391 2424 2391 Size: 33 7102 BamHI G'GATC_C Cuts at: 3527 3872 4465 4832 3527 Size: 345 593 367 5830 Fragments arranged by size: 5830 593 367 345 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2142 2627 2728 4456 5911 Size: 325 217 627 355 485 101 1728 1455 Cuts at: 5911 618 Size: 1842 Fragments arranged by size: 1842 1728 1455 627 485 355 325 217 101 BanII G_rGCy'C Cuts at: 1391 2070 2138 2601 3003 3856 4454 1391 Size: 679 68 463 402 853 598 4072 Fragments arranged by size: 4072 853 679 598 463 402 68 BbeI G_GCGC'C Cuts at: 2146 2146 Size: 7135 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3695 4226 928 Size: 971 1796 531 3837 Fragments arranged by size: 3837 1796 971 531 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5326 5360 1903 Size: 34 3389 34 3678 Fragments arranged by size: 3678 3389 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5027 6237 5027 Size: 1210 5925 BclI T'GATC_A Cuts at: 1548 2697 3267 1548 Size: 1149 570 5416 Fragments arranged by size: 5416 1149 570 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2160 2201 5746 Size: 107 122 71 631 605 488 41 3545 Cuts at: 5746 136 Size: 1525 Fragments arranged by size: 3545 1525 631 605 488 122 107 71 41 BglII A'GATC_T Cuts at: 2893 4126 7130 2893 Size: 1233 3004 2898 Fragments arranged by size: 3004 2898 1233 BlpI GC'TnA_GC Cuts at: 2051 2981 4552 2051 Size: 930 1571 4634 Fragments arranged by size: 4634 1571 930 Bme1580I G_kGCm'C Cuts at: 1790 2138 2630 5196 6352 1790 Size: 348 492 2566 1156 2573 Fragments arranged by size: 2573 2566 1156 492 348 BmgBI CAC'GTC Cuts at: 1318 2542 2779 3693 4255 1318 Size: 1224 237 914 562 4198 Fragments arranged by size: 4198 1224 914 562 237 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3874 4038 4866 Size: 416 53 83 399 24 2457 164 828 Cuts at: 4866 5824 442 Size: 958 1753 Fragments arranged by size: 2457 1753 958 828 416 399 164 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7135 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2585 2678 3068 3858 4105 4196 5795 1239 Size: 1346 93 390 790 247 91 1599 2579 Fragments arranged by size: 2579 1599 1346 790 390 247 93 91 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2368 2465 5260 6125 6423 2368 Size: 97 2795 865 298 3080 Fragments arranged by size: 3080 2795 865 298 97 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2550 5798 882 Size: 617 1051 3248 2219 Fragments arranged by size: 3248 2219 1051 617 BsaAI yAC'GTr Cuts at: 493 1204 4110 4432 4926 493 Size: 711 2906 322 494 2702 Fragments arranged by size: 2906 2702 711 494 322 BsaBI GATnn'nnATC Cuts at: 4281 7129 4281 Size: 2848 4287 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2143 3915 4942 5324 Size: 53 83 186 936 610 1772 1027 382 Cuts at: 5324 5959 275 Size: 635 1451 Fragments arranged by size: 1772 1451 1027 936 635 610 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2799 5568 5966 6240 6387 6798 2799 Size: 2769 398 274 147 411 3136 Fragments arranged by size: 3136 2769 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4116 4146 1178 Size: 30 165 30 2713 30 4167 Fragments arranged by size: 4167 2713 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2593 3149 3439 3494 1186 Size: 1407 556 290 55 4827 Fragments arranged by size: 4827 1407 556 290 55 BseYI C'CCAG_C Cuts at: 3088 4167 6338 6834 3088 Size: 1079 2171 496 3389 Fragments arranged by size: 3389 2171 1079 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2837 3008 1163 Size: 573 1101 171 5290 Fragments arranged by size: 5290 1101 573 171 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2038 4585 5346 6374 1540 Size: 275 186 37 2547 761 1028 2301 Fragments arranged by size: 2547 2301 1028 761 275 186 37 BsiHKAI G_wGCw'C Cuts at: 3003 3600 5196 5281 6352 3003 Size: 597 1596 85 1071 3786 Fragments arranged by size: 3786 1596 1071 597 85 BsmI GAATG_Cn' Cuts at: 4646 4739 4646 Size: 93 7042 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2138 2601 2630 3003 3600 3856 Size: 399 280 68 463 29 373 597 256 Cuts at: 3856 4454 5196 5281 6352 1391 Size: 598 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 598 597 463 399 373 280 256 85 68 29 BspEI T'CCGG_A Cuts at: 2799 6798 2799 Size: 3999 3136 BspHI T'CATG_A Cuts at: 2295 2472 2700 3108 5024 6754 2295 Size: 177 228 408 1916 1730 2676 Fragments arranged by size: 2676 1916 1730 408 228 177 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2322 3244 3381 4202 4490 844 Size: 1478 922 137 821 288 3489 Fragments arranged by size: 3489 1478 922 821 288 137 BsrBI CCG'CTC Cuts at: 1394 1823 2132 5022 1394 Size: 429 309 2890 3507 Fragments arranged by size: 3507 2890 429 309 BsrDI GCAATG_nn' Cuts at: 65 1248 5630 5804 65 Size: 1183 4382 174 1396 Fragments arranged by size: 4382 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2037 2166 2195 5779 5966 1700 Size: 110 227 129 29 3584 187 2869 Fragments arranged by size: 3584 2869 227 187 129 110 29 BsrGI T'GTAC_A Cuts at: 96 2544 2760 3384 96 Size: 2448 216 624 3847 Fragments arranged by size: 3847 2448 624 216 BssHII G'CGCG_C Cuts at: 1925 2030 2041 2204 2216 1925 Size: 105 11 163 12 6844 Fragments arranged by size: 6844 163 105 12 11 BssSI C'ACGA_G Cuts at: 3722 5195 6207 3722 Size: 1473 1012 4650 Fragments arranged by size: 4650 1473 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3826 794 Size: 3032 4103 BstBI TT'CG_AA Cuts at: 2007 5939 2007 Size: 3932 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7135 BstYI r'GATC_y Cuts at: 1972 2893 3315 3527 3872 4126 4465 4832 5217 Size: 921 422 212 345 254 339 367 385 Cuts at: 5217 5234 6686 6772 6784 7130 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 921 422 385 367 346 345 339 254 212 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2576 2576 Size: 7135 BtgI C'CryG_G Cuts at: 513 1065 2022 2138 2328 2631 513 Size: 552 957 116 190 303 5017 Fragments arranged by size: 5017 957 552 303 190 116 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2226 2993 3964 487 Size: 683 56 1000 767 971 3658 Fragments arranged by size: 3658 1000 971 767 683 56 BtsI GCAGTG_nn' Cuts at: 2825 4651 5445 5465 2825 Size: 1826 794 20 4495 Fragments arranged by size: 4495 1826 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7135 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7100 DraI TTT'AAA Cuts at: 4439 4439 Size: 7135 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3245 4336 6142 809 Size: 507 1929 1091 1806 1802 Fragments arranged by size: 1929 1806 1802 1091 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2035 2234 Size: 54 1425 50 275 18 195 10 199 Cuts at: 2234 2331 3190 3501 4582 5471 7100 8 Size: 97 859 311 1081 889 1629 43 Fragments arranged by size: 1629 1425 1081 889 859 311 275 199 195 97 54 50 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 2035 4582 1537 Size: 275 223 2547 4090 Fragments arranged by size: 4090 2547 275 223 EarI CTCTTCn'nnn_ Cuts at: 1353 2288 3199 3584 3908 5065 1353 Size: 935 911 385 324 1157 3423 Fragments arranged by size: 3423 1157 935 911 385 324 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2993 3208 5722 6096 6242 387 Size: 1064 138 1404 215 2514 374 146 1280 Fragments arranged by size: 2514 1404 1280 1064 374 215 146 138 EcoICRI GAG'CTC Cuts at: 3001 3001 Size: 7135 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2585 2678 3068 3575 3858 3929 Size: 393 512 441 93 390 507 283 71 Cuts at: 3929 3985 4105 4196 5198 5795 6582 6850 1239 Size: 56 120 91 1002 597 787 268 1524 Fragments arranged by size: 1524 1002 787 597 512 507 441 393 390 283 268 120 93 91 71 56 EcoNI CCTnn'n_nnAGG Cuts at: 3109 3204 3109 Size: 95 7040 EcoO109I rG'GnC_Cy Cuts at: 2687 3079 2687 Size: 392 6743 EcoRI G'AATT_C Cuts at: 3938 4444 4822 3938 Size: 506 378 6251 Fragments arranged by size: 6251 506 378 EcoRV GAT'ATC Cuts at: 2994 2994 Size: 7135 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2485 2485 Size: 7135 FspI TGC'GCA Cuts at: 5641 5641 Size: 7135 HaeII r_GCGC'y Cuts at: 1678 2146 6282 1678 Size: 468 4136 2531 Fragments arranged by size: 4136 2531 468 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3282 3314 3499 3531 4116 Size: 32 135 32 1710 32 185 32 585 Cuts at: 4116 4148 5776 5808 5850 5882 1373 Size: 32 1628 32 42 32 2626 Fragments arranged by size: 2626 1710 1628 585 185 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3187 3919 4479 4725 669 Size: 988 281 1249 732 560 246 3079 Fragments arranged by size: 3079 1249 988 732 560 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7135 HpaI GTT'AAC Cuts at: 4725 4725 Size: 7135 KasI G'GCGC_C Cuts at: 2142 2142 Size: 7135 KpnI G_GTAC'C Cuts at: 4460 4460 Size: 7135 MfeI C'AATT_G Cuts at: 4734 4734 Size: 7135 MluI A'CGCG_T Cuts at: 3432 7011 3432 Size: 3579 3556 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3774 6249 6433 1305 Size: 2469 2475 184 2007 Fragments arranged by size: 2475 2469 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3503 10 Size: 54 1425 343 1671 3642 Fragments arranged by size: 3642 1671 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3248 3263 3662 5093 5452 5611 518 Size: 648 2082 15 399 1431 359 159 2042 Fragments arranged by size: 2082 2042 1431 648 399 359 159 15 MspA1I CmG'CkG Cuts at: 1606 3022 3088 4351 4551 5228 6376 6621 1606 Size: 1416 66 1263 200 677 1148 245 2120 Fragments arranged by size: 2120 1416 1263 1148 677 245 200 66 NaeI GCC'GGC Cuts at: 2039 2197 2039 Size: 158 6977 NarI GG'CG_CC Cuts at: 2143 2143 Size: 7135 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5626 Fragments arranged by size: 5626 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7135 NgoMIV G'CCGG_C Cuts at: 2037 2195 2037 Size: 158 6977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7135 NotI GC'GGCC_GC Cuts at: 4582 4582 Size: 7135 NspI r_CATG'y Cuts at: 1132 1229 2491 2980 3661 3820 4493 6038 1132 Size: 97 1262 489 681 159 673 1545 2229 Fragments arranged by size: 2229 1545 1262 681 673 489 159 97 PasI CC'CwG_GG Cuts at: 1348 3581 1348 Size: 2233 4902 PciI A'CATG_T Cuts at: 3657 6034 3657 Size: 2377 4758 PflMI CCAn_nnn'nTGG Cuts at: 1108 2503 3469 1108 Size: 1395 966 4774 Fragments arranged by size: 4774 1395 966 PfoI T'CCnGG_A Cuts at: 2751 3366 2751 Size: 615 6520 PmeI GTTT'AAAC Cuts at: 4439 4439 Size: 7135 PmlI CAC'GTG Cuts at: 4110 4110 Size: 7135 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5204 5236 6717 6749 5204 Size: 32 1481 32 5590 Fragments arranged by size: 5590 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3079 3079 Size: 7135 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7135 PsiI TTA'TAA Cuts at: 4119 4705 4119 Size: 586 6549 PspOMI G'GGCC_C Cuts at: 2134 2134 Size: 7135 PstI C_TGCA'G Cuts at: 830 2319 4487 830 Size: 1489 2168 3478 Fragments arranged by size: 3478 2168 1489 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7135 PvuII CAG'CTG Cuts at: 3022 3088 4351 3022 Size: 66 1263 5806 Fragments arranged by size: 5806 1263 66 RsrII CG'GwC_CG Cuts at: 2169 2169 Size: 7135 SacI G_AGCT'C Cuts at: 3003 3003 Size: 7135 SalI G'TCGA_C Cuts at: 1936 3917 4477 1936 Size: 1981 560 4594 Fragments arranged by size: 4594 1981 560 SapI GCTCTTCn'nnn_ Cuts at: 3908 3908 Size: 7135 SbfI CC_TGCA'GG Cuts at: 4487 4487 Size: 7135 ScaI AGT'ACT Cuts at: 5383 7095 5383 Size: 1712 5423 SfcI C'TryA_G Cuts at: 826 1045 2315 2338 3933 4483 5618 6299 6490 Size: 219 1270 23 1595 550 1135 681 191 Cuts at: 6490 826 Size: 1471 Fragments arranged by size: 1595 1471 1270 1135 681 550 219 191 23 SfoI GGC'GCC Cuts at: 2144 2144 Size: 7135 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7135 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7135 SmaI CCC'GGG Cuts at: 1872 2111 4462 1872 Size: 239 2351 4545 Fragments arranged by size: 4545 2351 239 SmlI C'TyrA_G Cuts at: 820 1017 1943 2383 2480 5239 6140 6402 820 Size: 197 926 440 97 2759 901 262 1553 Fragments arranged by size: 2759 1553 926 901 440 262 197 97 SnaBI TAC'GTA Cuts at: 493 4432 4926 493 Size: 3939 494 2702 Fragments arranged by size: 3939 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7135 SphI G_CATG'C Cuts at: 1132 4493 1132 Size: 3361 3774 SspI AAT'ATT Cuts at: 5 52 5059 5 Size: 47 5007 2081 Fragments arranged by size: 5007 2081 47 StuI AGG'CCT Cuts at: 1517 2622 1517 Size: 1105 6030 StyI C'CwwG_G Cuts at: 513 1065 2022 3234 4162 4574 513 Size: 552 957 1212 928 412 3074 Fragments arranged by size: 3074 1212 957 928 552 412 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2783 4130 5162 5179 5332 5517 5962 Size: 1255 1203 1347 1032 17 153 185 445 Cuts at: 5962 325 Size: 1498 Fragments arranged by size: 1498 1347 1255 1203 1032 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2484 2544 2760 Size: 274 80 33 51 1443 507 60 216 Cuts at: 2760 2958 3360 3384 4299 5381 7093 96 Size: 198 402 24 915 1082 1712 138 Fragments arranged by size: 1712 1443 1082 915 507 402 274 216 198 138 80 60 51 33 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7095 7127 7095 Size: 32 7103 Tth111I GACn'n_nGTC Cuts at: 1316 3245 3923 1316 Size: 1929 678 4528 Fragments arranged by size: 4528 1929 678 XbaI T'CTAG_A Cuts at: 4471 4471 Size: 7135 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2638 4348 2638 Size: 1710 5425 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7135 XmaI C'CCGG_G Cuts at: 1870 2109 4460 1870 Size: 239 2351 4545 Fragments arranged by size: 4545 2351 239 XmnI GAAnn'nnTTC Cuts at: 2468 3942 5264 2468 Size: 1474 1322 4339 Fragments arranged by size: 4339 1474 1322 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3916 4943 276 Size: 53 83 186 936 2382 1027 2468 Fragments arranged by size: 2468 2382 1027 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII FseI FspAI NruI NsiI PacI PspXI PsrI SacII SanDI SexAI SfiI SrfI SwaI