(Circular) (Six-base) MAPSORT of: pFN21ASDA0963.seq Check: 3673 from: 1 to: 8827 pFN21ASDA0963 8827 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2919 5083 5119 2919 Size: 2164 36 6627 Fragments arranged by size: 6627 2164 36 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5577 6637 278 Size: 53 83 186 936 4041 1060 2468 Fragments arranged by size: 4041 2468 1060 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3273 3528 6170 1937 Size: 1336 255 2642 4594 Fragments arranged by size: 4594 2642 1336 255 Acc65I G'GTAC_C Cuts at: 6148 6148 Size: 8827 AclI AA'CG_TT Cuts at: 1563 6954 7327 1563 Size: 5391 373 3063 Fragments arranged by size: 5391 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3843 4184 5186 5630 6890 8274 8542 1632 Size: 2211 341 1002 444 1260 1384 268 1917 Fragments arranged by size: 2211 1917 1384 1260 1002 444 341 268 AfeI AGC'GCT Cuts at: 3647 4382 3647 Size: 735 8092 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8630 AflIII A'CryG_T Cuts at: 1203 3567 7726 8703 1203 Size: 2364 4159 977 1327 Fragments arranged by size: 4159 2364 1327 977 AgeI A'CCGG_T Cuts at: 4548 7658 4548 Size: 3110 5717 AhdI GACnn_n'nnGTC Cuts at: 2362 7556 2362 Size: 5194 3633 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8827 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3026 3248 5609 1184 Size: 1842 222 2361 4402 Fragments arranged by size: 4402 2361 1842 222 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4598 4630 4598 Size: 32 8795 AlwNI CAG_nnn'CTG Cuts at: 3139 4174 4345 4846 5445 5497 5683 6570 8142 Size: 1035 171 501 599 52 186 887 1572 Cuts at: 8142 3139 Size: 3824 Fragments arranged by size: 3824 1572 1035 887 599 501 186 171 52 ApaI G_GGCC'C Cuts at: 2040 2761 4657 4738 5210 2040 Size: 721 1896 81 472 5657 Fragments arranged by size: 5657 1896 721 472 81 ApaLI G'TGCA_C Cuts at: 3011 4484 4952 6884 8040 3011 Size: 1473 468 1932 1156 3798 Fragments arranged by size: 3798 1932 1473 1156 468 ApoI r'AATT_y Cuts at: 1493 6136 6362 6514 8623 1493 Size: 4643 226 152 2109 1697 Fragments arranged by size: 4643 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7381 160 Size: 872 6349 1606 Fragments arranged by size: 6349 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8827 AvaI C'yCGr_G Cuts at: 1870 1943 3702 3915 4034 4136 4432 4731 5108 Size: 73 1759 213 119 102 296 299 377 Cuts at: 5108 5651 6062 6152 1870 Size: 543 411 90 4545 Fragments arranged by size: 4545 1759 543 411 377 299 296 213 119 102 90 73 BamHI G'GATC_C Cuts at: 4100 6157 6524 4100 Size: 2057 367 6403 Fragments arranged by size: 6403 2057 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3290 3404 3795 4476 4727 Size: 325 217 627 1503 114 391 681 251 Cuts at: 4727 5112 5508 5719 5854 5897 6104 6148 7603 Size: 385 396 211 135 43 207 44 1455 Cuts at: 7603 618 Size: 1842 Fragments arranged by size: 1842 1503 1455 681 627 396 391 385 325 251 217 211 207 135 114 44 43 BanII G_rGCy'C Cuts at: 1391 2040 2204 2761 4083 4233 4657 4738 5210 Size: 649 164 557 1322 150 424 81 472 Cuts at: 5210 5397 6146 1391 Size: 187 749 4072 Fragments arranged by size: 4072 1322 749 649 557 472 424 187 164 150 81 BbeI G_GCGC'C Cuts at: 3408 4480 5723 5858 6108 3408 Size: 1072 1243 135 250 6127 Fragments arranged by size: 6127 1243 1072 250 135 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4573 5128 5618 928 Size: 971 2674 555 490 4137 Fragments arranged by size: 4137 2674 971 555 490 BbvCI CC'TCA_GC Cuts at: 2586 4697 2586 Size: 2111 6716 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2423 2457 3104 3138 3433 3467 3708 Size: 34 486 34 647 34 295 34 241 Cuts at: 3708 3742 7018 7052 1903 Size: 34 3276 34 3678 Fragments arranged by size: 3678 3276 647 486 295 241 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4793 6719 7929 4793 Size: 1926 1210 5691 Fragments arranged by size: 5691 1926 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8827 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2255 4197 5567 Size: 107 122 71 631 605 583 1942 1370 Cuts at: 5567 7438 136 Size: 1871 1525 Fragments arranged by size: 1942 1871 1525 1370 631 605 583 122 107 71 BglII A'GATC_T Cuts at: 5073 8822 5073 Size: 3749 5078 BlpI GC'TnA_GC Cuts at: 2474 3020 3464 6244 2474 Size: 546 444 2780 5057 Fragments arranged by size: 5057 2780 546 444 Bme1580I G_kGCm'C Cuts at: 1790 2040 2616 2761 3015 3350 3798 4488 4657 Size: 250 576 145 254 335 448 690 169 Cuts at: 4657 4738 4794 4956 5115 5210 5900 6888 8044 Size: 81 56 162 159 95 690 988 1156 Cuts at: 8044 1790 Size: 2573 Fragments arranged by size: 2573 1156 988 690 690 576 448 335 254 250 169 162 159 145 95 81 56 BmgBI CAC'GTC Cuts at: 1318 4897 5704 1318 Size: 3579 807 4441 Fragments arranged by size: 4441 3579 807 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4080 6558 7516 Size: 416 53 83 399 24 2663 2478 958 Cuts at: 7516 442 Size: 1753 Fragments arranged by size: 2663 2478 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8827 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2341 2341 Size: 8827 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2161 2251 2921 3620 4166 4411 4661 4827 Size: 922 90 670 699 546 245 250 166 Cuts at: 4827 4967 5354 5990 7487 1239 Size: 140 387 636 1497 2579 Fragments arranged by size: 2579 1497 922 699 670 636 546 387 250 245 166 140 90 Bpu10I CC'TnA_GC Cuts at: 2298 2586 4697 4763 2298 Size: 288 2111 66 6362 Fragments arranged by size: 6362 2111 288 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2942 6952 7817 8115 2942 Size: 4010 865 298 3654 Fragments arranged by size: 4010 3654 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2615 2681 4116 4567 4662 7490 882 Size: 617 1116 66 1435 451 95 2828 2219 Fragments arranged by size: 2828 2219 1435 1116 617 451 95 66 BsaAI yAC'GTr Cuts at: 493 1204 2638 4360 6124 6618 493 Size: 711 1434 1722 1764 494 2702 Fragments arranged by size: 2702 1764 1722 1434 711 494 BsaBI GATnn'nnATC Cuts at: 8821 8821 Size: 8827 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3060 3168 3405 3477 Size: 53 83 186 936 1527 108 237 72 Cuts at: 3477 3963 4477 5178 5574 5720 5855 5871 5883 Size: 486 514 701 396 146 135 16 12 Cuts at: 5883 5907 6105 6634 7016 7651 275 Size: 24 198 529 382 635 1451 Fragments arranged by size: 1527 1451 936 701 635 529 514 486 396 382 237 198 186 146 135 108 83 72 53 24 16 12 BsaWI w'CCGG_w Cuts at: 3357 4548 7260 7658 7932 8079 8490 3357 Size: 1191 2712 398 274 147 411 3694 Fragments arranged by size: 3694 2712 1191 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4010 4040 4490 4520 4547 Size: 30 165 30 2607 30 450 30 27 Cuts at: 4547 4577 4598 4628 1178 Size: 30 21 30 5377 Fragments arranged by size: 5377 2607 450 165 30 30 30 30 30 30 27 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2296 2335 2585 2588 2603 2606 2609 2618 Size: 1110 39 250 3 15 3 3 9 Cuts at: 2618 3383 3509 3722 3900 4018 5084 5096 5396 Size: 765 126 213 178 118 1066 12 300 Cuts at: 5396 6105 1186 Size: 709 3908 Fragments arranged by size: 3908 1110 1066 765 709 300 250 213 178 126 118 39 15 12 9 3 3 3 BseYI C'CCAG_C Cuts at: 2608 2822 3511 3778 4195 4243 4254 5255 5893 Size: 214 689 267 417 48 11 1001 638 Cuts at: 5893 6019 8030 8526 2608 Size: 126 2011 496 2909 Fragments arranged by size: 2909 2011 1001 689 638 496 417 267 214 126 48 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2111 2576 3362 4724 5975 1163 Size: 573 375 465 786 1362 1251 4015 Fragments arranged by size: 4015 1362 1251 786 573 465 375 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2920 3585 3819 4096 4456 4519 Size: 275 186 919 665 234 277 360 63 Cuts at: 4519 4540 5320 5817 6277 7038 8066 1540 Size: 21 780 497 460 761 1028 2301 Fragments arranged by size: 2301 1028 919 780 761 665 497 460 360 277 275 234 186 63 21 BsiHKAI G_wGCw'C Cuts at: 2204 3015 4233 4488 4956 6888 6973 8044 2204 Size: 811 1218 255 468 1932 85 1071 2987 Fragments arranged by size: 2987 1932 1218 1071 811 468 255 85 BsmI GAATG_Cn' Cuts at: 3208 4969 6338 6431 3208 Size: 1761 1369 93 5604 Fragments arranged by size: 5604 1761 1369 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2950 3817 2950 Size: 867 7960 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2204 2616 2761 3015 3350 3798 Size: 399 250 164 412 145 254 335 448 Cuts at: 3798 4083 4233 4488 4657 4738 4794 4956 5115 Size: 285 150 255 169 81 56 162 159 Cuts at: 5115 5210 5397 5900 6146 6888 6973 8044 1391 Size: 95 187 503 246 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 503 448 412 399 335 285 255 254 250 246 187 169 164 162 159 150 145 95 85 81 56 BspEI T'CCGG_A Cuts at: 3357 8490 3357 Size: 5133 3694 BspHI T'CATG_A Cuts at: 2196 4386 6716 8446 2196 Size: 2190 2330 1730 2577 Fragments arranged by size: 2577 2330 2190 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2294 2919 2988 3249 5083 5119 5601 6182 Size: 1450 625 69 261 1834 36 482 581 Cuts at: 6182 844 Size: 3489 Fragments arranged by size: 3489 1834 1450 625 581 482 261 69 36 BsrBI CCG'CTC Cuts at: 1394 1823 3890 4150 4159 4392 6067 6714 1394 Size: 429 2067 260 9 233 1675 647 3507 Fragments arranged by size: 3507 2067 1675 647 429 260 233 9 BsrDI GCAATG_nn' Cuts at: 65 1248 7322 7496 65 Size: 1183 6074 174 1396 Fragments arranged by size: 6074 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2077 2395 2861 3104 3206 3684 4191 Size: 110 267 318 466 243 102 478 507 Cuts at: 4191 4274 4548 4611 5319 5451 5460 5873 7471 Size: 83 274 63 708 132 9 413 1598 Cuts at: 7471 7658 1700 Size: 187 2869 Fragments arranged by size: 2869 1598 708 507 478 466 413 318 274 267 243 187 132 110 102 83 63 9 BsrGI T'GTAC_A Cuts at: 96 2094 3447 5301 96 Size: 1998 1353 1854 3622 Fragments arranged by size: 3622 1998 1854 1353 BssHII G'CGCG_C Cuts at: 1925 2833 3761 5529 6015 1925 Size: 908 928 1768 486 4737 Fragments arranged by size: 4737 1768 928 908 486 BssSI C'ACGA_G Cuts at: 2807 4403 6887 7899 2807 Size: 1596 2484 1012 3735 Fragments arranged by size: 3735 2484 1596 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2554 4174 4846 5497 5602 5683 794 Size: 1760 1620 672 651 105 81 3938 Fragments arranged by size: 3938 1760 1620 672 651 105 81 BstBI TT'CG_AA Cuts at: 2007 7631 2007 Size: 5624 3203 BstEII G'GTnAC_C Cuts at: 1184 5371 1184 Size: 4187 4640 BstXI CCAn_nnnn'nTGG Cuts at: 3107 3220 4639 5194 3107 Size: 113 1419 555 6740 Fragments arranged by size: 6740 1419 555 113 BstYI r'GATC_y Cuts at: 1972 3135 3498 4100 4171 5073 5646 6157 6524 Size: 1163 363 602 71 902 573 511 367 Cuts at: 6524 6909 6926 8378 8464 8476 8822 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1163 902 602 573 511 385 367 363 346 86 71 17 12 Bsu36I CC'TnA_GG Cuts at: 4367 4367 Size: 8827 BtgI C'CryG_G Cuts at: 513 1065 2043 2253 2352 2997 3408 3423 3750 Size: 552 978 210 99 645 411 15 327 Cuts at: 3750 4505 4663 4680 5158 5242 5281 5376 513 Size: 755 158 17 478 84 39 95 3964 Fragments arranged by size: 3964 978 755 645 552 478 411 327 210 158 99 95 84 39 17 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2868 2989 3436 3652 5539 487 Size: 683 56 1642 121 447 216 1887 3775 Fragments arranged by size: 3775 1887 1642 683 447 216 121 56 BtsI GCAGTG_nn' Cuts at: 2037 2128 5428 5455 6343 7137 7157 2037 Size: 91 3300 27 888 794 20 3707 Fragments arranged by size: 3707 3300 888 794 91 27 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8827 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8792 DraI TTT'AAA Cuts at: 6131 6131 Size: 8827 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3434 4058 4460 7834 809 Size: 507 2118 624 402 3374 1802 Fragments arranged by size: 3374 2118 1802 624 507 402 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2256 2886 2917 Size: 54 1425 50 275 18 426 630 31 Cuts at: 2917 3109 3420 3582 3816 4407 4537 4599 4629 Size: 192 311 162 234 591 130 62 30 Cuts at: 4629 4867 5284 5317 5568 5814 6274 7163 8792 Size: 238 417 33 251 246 460 889 1629 Cuts at: 8792 8 Size: 43 Fragments arranged by size: 1629 1425 889 630 591 460 426 417 311 275 251 246 238 234 192 162 130 62 54 50 43 33 31 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2917 3582 3816 4537 5317 5814 6274 Size: 275 1105 665 234 721 780 497 460 Cuts at: 6274 1537 Size: 4090 Fragments arranged by size: 4090 1105 780 721 665 497 460 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 3071 3386 6757 1353 Size: 1718 315 3371 3423 Fragments arranged by size: 3423 3371 1718 315 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2194 2615 3070 4280 5285 5342 Size: 1064 138 605 421 455 1210 1005 57 Cuts at: 5342 7414 7788 7934 387 Size: 2072 374 146 1280 Fragments arranged by size: 2072 1280 1210 1064 1005 605 455 421 374 146 138 57 EcoICRI GAG'CTC Cuts at: 2202 4231 2202 Size: 2029 6798 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2161 2251 2921 3620 3843 4166 4184 Size: 393 529 90 670 699 223 323 18 Cuts at: 4184 4411 4661 4827 4967 5186 5354 5630 5990 Size: 227 250 166 140 219 168 276 360 Cuts at: 5990 6890 7487 8274 8542 1239 Size: 900 597 787 268 1524 Fragments arranged by size: 1524 900 787 699 670 597 529 393 360 323 276 268 250 227 223 219 168 166 140 90 18 EcoNI CCTnn'n_nnAGG Cuts at: 2411 2411 Size: 8827 EcoO109I rG'GnC_Cy Cuts at: 2036 2757 2758 2812 3559 4141 4653 4654 4735 Size: 721 1 54 747 582 512 1 81 Cuts at: 4735 4813 4843 5207 5338 2036 Size: 78 30 364 131 5525 Fragments arranged by size: 5525 747 721 582 512 364 131 81 78 54 30 1 1 EcoRI G'AATT_C Cuts at: 6136 6514 6136 Size: 378 8449 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4399 4726 4399 Size: 327 8500 FspI TGC'GCA Cuts at: 7333 7333 Size: 8827 HaeII r_GCGC'y Cuts at: 1678 2121 3285 3408 3649 4384 4480 4515 4612 Size: 443 1164 123 241 735 96 35 97 Cuts at: 4612 4639 5201 5445 5723 5768 5858 6108 7974 Size: 27 562 244 278 45 90 250 1866 Cuts at: 7974 1678 Size: 2531 Fragments arranged by size: 2531 1866 1164 735 562 443 278 250 244 241 123 97 96 90 45 35 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2348 2380 4010 4042 4085 Size: 32 135 32 776 32 1630 32 43 Cuts at: 4085 4117 7468 7500 7542 7574 1373 Size: 32 3351 32 42 32 2626 Fragments arranged by size: 3351 2626 1630 776 135 43 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4216 6171 6417 669 Size: 988 281 2278 1955 246 3079 Fragments arranged by size: 3079 2278 1955 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8827 HpaI GTT'AAC Cuts at: 6417 6417 Size: 8827 KasI G'GCGC_C Cuts at: 3404 4476 5719 5854 6104 3404 Size: 1072 1243 135 250 6127 Fragments arranged by size: 6127 1243 1072 250 135 KpnI G_GTAC'C Cuts at: 6152 6152 Size: 8827 MfeI C'AATT_G Cuts at: 6426 6426 Size: 8827 MluI A'CGCG_T Cuts at: 8703 8703 Size: 8827 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2346 2352 2973 3978 4578 7941 8125 1305 Size: 1041 6 621 1005 600 3363 184 2007 Fragments arranged by size: 3363 2007 1041 1005 621 600 184 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 2258 3111 3422 4409 4631 Size: 54 1425 343 426 853 311 987 222 Cuts at: 4631 10 Size: 4206 Fragments arranged by size: 4206 1425 987 853 426 343 311 222 54 MslI CAynn'nnrTG Cuts at: 518 1166 3008 3218 3428 5009 6785 7144 7303 Size: 648 1842 210 210 1581 1776 359 159 Cuts at: 7303 518 Size: 2042 Fragments arranged by size: 2042 1842 1776 1581 648 359 210 210 159 MspA1I CmG'CkG Cuts at: 1606 2754 2826 3441 3505 3821 3959 4243 4258 Size: 1148 72 615 64 316 138 284 15 Cuts at: 4258 4474 5160 5244 5259 5283 5332 5378 5761 Size: 216 686 84 15 24 49 46 383 Cuts at: 5761 5860 5893 6243 6920 8068 8313 1606 Size: 99 33 350 677 1148 245 2120 Fragments arranged by size: 2120 1148 1148 686 677 615 383 350 316 284 245 216 138 99 84 72 64 49 46 33 24 15 15 NaeI GCC'GGC Cuts at: 2079 2397 2863 3208 3686 4193 5321 5462 5875 Size: 318 466 345 478 507 1128 141 413 Cuts at: 5875 2079 Size: 5031 Fragments arranged by size: 5031 1128 507 478 466 413 345 318 141 NarI GG'CG_CC Cuts at: 3405 4477 5720 5855 6105 3405 Size: 1072 1243 135 250 6127 Fragments arranged by size: 6127 1243 1072 250 135 NcoI C'CATG_G Cuts at: 513 1065 2043 3408 3423 513 Size: 552 978 1365 15 5917 Fragments arranged by size: 5917 1365 978 552 15 NdeI CA'TA_TG Cuts at: 387 387 Size: 8827 NgoMIV G'CCGG_C Cuts at: 2077 2395 2861 3206 3684 4191 5319 5460 5873 Size: 318 466 345 478 507 1128 141 413 Cuts at: 5873 2077 Size: 5031 Fragments arranged by size: 5031 1128 507 478 466 413 345 318 141 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8827 NotI GC'GGCC_GC Cuts at: 6274 6274 Size: 8827 NspI r_CATG'y Cuts at: 1132 1229 3448 5521 5980 6185 7730 1132 Size: 97 2219 2073 459 205 1545 2229 Fragments arranged by size: 2229 2219 2073 1545 459 205 97 PasI CC'CwG_GG Cuts at: 1348 4795 5397 5728 1348 Size: 3447 602 331 4447 Fragments arranged by size: 4447 3447 602 331 PciI A'CATG_T Cuts at: 7726 7726 Size: 8827 PflMI CCAn_nnn'nTGG Cuts at: 1108 2427 4261 1108 Size: 1319 1834 5674 Fragments arranged by size: 5674 1834 1319 PfoI T'CCnGG_A Cuts at: 2898 3138 4143 4827 4877 5046 2898 Size: 240 1005 684 50 169 6679 Fragments arranged by size: 6679 1005 684 240 169 50 PmeI GTTT'AAAC Cuts at: 6131 6131 Size: 8827 PmlI CAC'GTG Cuts at: 4360 4360 Size: 8827 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2135 2167 4598 4630 6896 6928 8409 8441 2135 Size: 32 2431 32 2266 32 1481 32 2521 Fragments arranged by size: 2521 2431 2266 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2812 4141 4813 2812 Size: 1329 672 6826 Fragments arranged by size: 6826 1329 672 PshAI GACnn'nnGTC Cuts at: 1613 3186 1613 Size: 1573 7254 PsiI TTA'TAA Cuts at: 6397 6397 Size: 8827 PspOMI G'GGCC_C Cuts at: 2036 2757 4653 4734 5206 2036 Size: 721 1896 81 472 5657 Fragments arranged by size: 5657 1896 721 472 81 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3299 3331 3299 Size: 32 8795 PstI C_TGCA'G Cuts at: 830 2115 2775 3246 3747 4119 5435 5493 5598 Size: 1285 660 471 501 372 1316 58 105 Cuts at: 5598 5679 6179 830 Size: 81 500 3478 Fragments arranged by size: 3478 1316 1285 660 501 500 471 372 105 81 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8827 PvuII CAG'CTG Cuts at: 4243 5259 5332 4243 Size: 1016 73 7738 Fragments arranged by size: 7738 1016 73 SacI G_AGCT'C Cuts at: 2204 4233 2204 Size: 2029 6798 SacII CC_GC'GG Cuts at: 5161 5245 5284 5379 5161 Size: 84 39 95 8609 Fragments arranged by size: 8609 95 84 39 SalI G'TCGA_C Cuts at: 1936 6169 1936 Size: 4233 4594 SanDI GG'GwC_CC Cuts at: 4813 4813 Size: 8827 SbfI CC_TGCA'GG Cuts at: 5493 6179 5493 Size: 686 8141 ScaI AGT'ACT Cuts at: 4979 7075 8787 4979 Size: 2096 1712 5019 Fragments arranged by size: 5019 2096 1712 SexAI A'CCwGG_T Cuts at: 2412 4557 2412 Size: 2145 6682 SfcI C'TryA_G Cuts at: 826 1045 2111 2771 3242 3743 4115 5431 5489 Size: 219 1066 660 471 501 372 1316 58 Cuts at: 5489 5594 5675 5803 6175 7310 7991 8182 826 Size: 105 81 128 372 1135 681 191 1471 Fragments arranged by size: 1471 1316 1135 1066 681 660 501 471 372 372 219 191 128 105 81 58 SfoI GGC'GCC Cuts at: 3406 4478 5721 5856 6106 3406 Size: 1072 1243 135 250 6127 Fragments arranged by size: 6127 1243 1072 250 135 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8827 SgrAI Cr'CCGG_yG Cuts at: 1700 2077 1700 Size: 377 8450 SmaI CCC'GGG Cuts at: 1872 4138 4733 5110 6154 1872 Size: 2266 595 377 1044 4545 Fragments arranged by size: 4545 2266 1044 595 377 SmlI C'TyrA_G Cuts at: 820 1017 1943 2957 6931 7832 8094 820 Size: 197 926 1014 3974 901 262 1553 Fragments arranged by size: 3974 1553 1014 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6124 6618 493 Size: 5631 494 2702 Fragments arranged by size: 5631 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8827 SphI G_CATG'C Cuts at: 1132 6185 1132 Size: 5053 3774 SspI AAT'ATT Cuts at: 5 52 6751 5 Size: 47 6699 2081 Fragments arranged by size: 6699 2081 47 StuI AGG'CCT Cuts at: 1517 3158 4424 5822 1517 Size: 1641 1266 1398 4522 Fragments arranged by size: 4522 1641 1398 1266 StyI C'CwwG_G Cuts at: 513 1065 2043 3408 3423 3675 4494 5388 5616 Size: 552 978 1365 15 252 819 894 228 Cuts at: 5616 6266 513 Size: 650 3074 Fragments arranged by size: 3074 1365 978 894 819 650 552 252 228 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2468 2685 4559 6854 6871 7024 7209 Size: 1255 888 217 1874 2295 17 153 185 Cuts at: 7209 7654 325 Size: 445 1498 Fragments arranged by size: 2295 1874 1498 1255 888 445 217 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2094 3447 4704 Size: 274 80 33 51 1443 117 1353 1257 Cuts at: 4704 4977 5301 7073 8785 96 Size: 273 324 1772 1712 138 Fragments arranged by size: 1772 1712 1443 1353 1257 324 274 273 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3845 3877 4490 4522 8787 8819 3845 Size: 32 613 32 4265 32 3853 Fragments arranged by size: 4265 3853 613 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4058 1316 Size: 2742 6085 XbaI T'CTAG_A Cuts at: 6163 6163 Size: 8827 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 3415 4802 5263 2029 Size: 1386 1387 461 5593 Fragments arranged by size: 5593 1387 1386 461 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8827 XmaI C'CCGG_G Cuts at: 1870 4136 4731 5108 6152 1870 Size: 2266 595 377 1044 4545 Fragments arranged by size: 4545 2266 1044 595 377 XmnI GAAnn'nnTTC Cuts at: 2147 3569 6956 2147 Size: 1422 3387 4018 Fragments arranged by size: 4018 3387 1422 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5575 6635 276 Size: 53 83 186 936 4041 1060 2468 Fragments arranged by size: 4041 2468 1060 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PspXI RsrII SapI SfiI SrfI SwaI