Restriction map for
pFN21ASDA0945
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AvrII BciVI BclI BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI HgaI HincII HindII HindIII
HpaI Ksp632I KspI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NotI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 1770 GGTACC G^GTACC
AccII 2075 CGCG CG^CG
AcsI 1456 GAATTT [GA]^AATT[CT]
AflII 2323 CTTAAG C^TTAAG
AflIII 1716 ACGTGT A^C[GA][CT]GT
ApoI 1456 GAATTT [GA]^AATT[CT]
Asp718I 1770 GGTACC G^GTACC
AspI 1885 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1643 TGCGCA TGC^GCA
BamHI 447 GGATCC G^GATCC
BbrPI 1715 CACGTG CAC^GTG
BbvCI 1048 CCTCAGC CCTCAGC(-5/-2)
BcgI 1256 CGAGCTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2323 CTTAAG C^TTAAG
BglII 1940 AGATCT A^GATCT
BsaAI 1715 CACGTG [CT]AC^GT[GA]
BsaI 590 GAGACC GGTCTC(1/5)
BsrFI 241 GCCGGT [GA]^CCGG[CT]
BstAPI 1017 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 136 GGTCACC G^GT[ACGT]ACC
BstPI 136 GGTCACC G^GT[ACGT]ACC
BstUI 2075 CGCG CG^CG
Cfr10I 241 GCCGGT [GA]^CCGG[CT]
EcoNI 897 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 136 GGTCACC G^GT[ACGT]ACC
FspI 1643 TGCGCA TGC^GCA
KpnI 1770 GGTACC GGTAC^C
MaeII 1716 ACGT A^CGT
MseI 2324 TTAA T^TAA
MvnI 2075 CGCG CG^CG
NheI 613 GCTAGC G^CTAGC
NruI 2074 TCGCGA TCG^CGA
PflFI 1885 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 773 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1715 CACGTG CAC^GTG
PmlI 1715 CACGTG CAC^GTG
ScaI 2141 AGTACT AGT^ACT
SmlI 2323 CTTAAG C^T[CT][GA]AG
TaqI 438 TCGA T^CGA
Tru9I 2324 TTAA T^TAA
Tth111I 1885 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 438 TCGA T^CGA
Van91I 773 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1922 CCACACCTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
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AocI 1198 CCTGAGG CC^T[ACGT]AGG
1268 CCTCAGG
BbsI 76 GAAGAC GAAGAC(2/6)
319 GAAGAC
BpuAI 76 GAAGAC GAAGAC(2/6)
319 GAAGAC
BsrBI 652 GAGCGG CCGCTC(-3/-3)
1855 GAGCGG
Bsu36I 1198 CCTGAGG CC^T[ACGT]AGG
1268 CCTCAGG
Eco81I 1198 CCTGAGG CC^T[ACGT]AGG
1268 CCTCAGG
MslI 1329 CACTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2293 CATGTCTATG
NcoI 844 CCATGG C^CATGG
2159 CCATGG
NspI 119 GCATGC [GA]CATG^[CT]
2039 GCATGC
SacI 1114 GAGCTC GAGCT^C
1257 GAGCTC
SfiI 43 GGCCTCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
737 GGCCAAAGTGGCC
SphI 119 GCATGC GCATG^C
2039 GCATGC
Tsp509I 160 AATT ^AATT
1457 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BssSI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI StuI StyI TseI Tsp45I
TspRI XhoII