(Circular) (Six-base) MAPSORT of: pFN21ASDA0880.seq Check: 3143 from: 1 to: 6856 pFN21ASDA0880 6856 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2992 2992 Size: 6856 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2282 4666 278 Size: 53 83 186 936 746 2384 2468 Fragments arranged by size: 2468 2384 936 746 186 83 53 AccI GT'mk_AC Cuts at: 1937 4199 1937 Size: 2262 4594 Acc65I G'GTAC_C Cuts at: 2053 2062 4177 2053 Size: 9 2115 4732 Fragments arranged by size: 4732 2115 9 AclI AA'CG_TT Cuts at: 1563 4983 5356 1563 Size: 3420 373 3063 Fragments arranged by size: 3420 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2930 3373 4919 6303 6571 1632 Size: 1298 443 1546 1384 268 1917 Fragments arranged by size: 1917 1546 1384 1298 443 268 AfeI AGC'GCT Cuts at: 2258 2258 Size: 6856 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6659 AflIII A'CryG_T Cuts at: 1203 5755 6732 1203 Size: 4552 977 1327 Fragments arranged by size: 4552 1327 977 AgeI A'CCGG_T Cuts at: 3969 5687 3969 Size: 1718 5138 AhdI GACnn_n'nnGTC Cuts at: 5585 5585 Size: 6856 AleI CACnn'nnGTG Cuts at: 1166 3548 3899 1166 Size: 2382 351 4123 Fragments arranged by size: 4123 2382 351 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2372 2780 3680 3694 1184 Size: 1188 408 900 14 4346 Fragments arranged by size: 4346 1188 900 408 14 AlwNI CAG_nnn'CTG Cuts at: 2578 2614 4007 4599 6171 2578 Size: 36 1393 592 1572 3263 Fragments arranged by size: 3263 1572 1393 592 36 ApaI G_GGCC'C Cuts at: 3338 3913 3338 Size: 575 6281 ApaLI G'TGCA_C Cuts at: 2357 3902 4913 6069 2357 Size: 1545 1011 1156 3144 Fragments arranged by size: 3144 1545 1156 1011 ApoI r'AATT_y Cuts at: 1493 4165 4391 4543 6652 1493 Size: 2672 226 152 2109 1697 Fragments arranged by size: 2672 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5410 160 Size: 872 4378 1606 Fragments arranged by size: 4378 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6856 AvaI C'yCGr_G Cuts at: 1870 1943 2702 4143 4181 1870 Size: 73 759 1441 38 4545 Fragments arranged by size: 4545 1441 759 73 38 BamHI G'GATC_C Cuts at: 2706 3674 4186 4553 2706 Size: 968 512 367 5009 Fragments arranged by size: 5009 968 512 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 2062 2632 2843 3350 Size: 325 217 627 266 9 570 211 507 Cuts at: 3350 3706 4177 5632 618 Size: 356 471 1455 1842 Fragments arranged by size: 1842 1455 627 570 507 471 356 325 266 217 211 9 BanII G_rGCy'C Cuts at: 1391 2237 3023 3338 3737 3913 4175 1391 Size: 846 786 315 399 176 262 4072 Fragments arranged by size: 4072 846 786 399 315 262 176 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3091 3807 928 Size: 971 1192 716 3977 Fragments arranged by size: 3977 1192 971 716 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2654 2688 3868 3902 5047 5081 1903 Size: 34 717 34 1180 34 1145 34 3678 Fragments arranged by size: 3678 1180 1145 717 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4748 5958 4748 Size: 1210 5646 BclI T'GATC_A Cuts at: 1548 1548 Size: 6856 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3576 5467 136 Size: 107 122 71 631 605 1904 1891 1525 Fragments arranged by size: 1904 1891 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6851 6851 Size: 6856 BlpI GC'TnA_GC Cuts at: 4273 4273 Size: 6856 Bme1580I G_kGCm'C Cuts at: 1790 2096 2361 2637 3338 3355 3460 3711 3906 Size: 306 265 276 701 17 105 251 195 Cuts at: 3906 3913 4917 6073 1790 Size: 7 1004 1156 2573 Fragments arranged by size: 2573 1156 1004 701 306 276 265 251 195 105 17 7 BmgBI CAC'GTC Cuts at: 1318 2140 1318 Size: 822 6034 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3916 4587 5545 Size: 416 53 83 399 24 2499 671 958 Cuts at: 5545 442 Size: 1753 Fragments arranged by size: 2499 1753 958 671 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6856 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3517 3517 Size: 6856 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2130 2254 3236 3403 3491 3968 5516 1239 Size: 891 124 982 167 88 477 1548 2579 Fragments arranged by size: 2579 1548 982 891 477 167 124 88 Bpu10I CC'TnA_GC Cuts at: 3914 3914 Size: 6856 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2539 3568 4981 5846 6144 2539 Size: 1029 1413 865 298 3251 Fragments arranged by size: 3251 1413 1029 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5519 882 Size: 617 4020 2219 Fragments arranged by size: 4020 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4153 4647 493 Size: 711 2949 494 2702 Fragments arranged by size: 2949 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6850 6850 Size: 6856 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2279 3309 3925 4663 Size: 53 83 186 936 746 1030 616 738 Cuts at: 4663 5045 5680 275 Size: 382 635 1451 Fragments arranged by size: 1451 1030 936 746 738 635 616 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3969 5289 5687 5961 6108 6519 3969 Size: 1320 398 274 147 411 4306 Fragments arranged by size: 4306 1320 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3209 3239 3749 3779 1178 Size: 30 165 30 1806 30 510 30 4255 Fragments arranged by size: 4255 1806 510 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3801 1186 Size: 2615 4241 BseYI C'CCAG_C Cuts at: 2524 2571 2617 2752 2854 3364 3539 3588 3860 Size: 47 46 135 102 510 175 49 272 Cuts at: 3860 6059 6555 2524 Size: 2199 496 2825 Fragments arranged by size: 2825 2199 510 496 272 175 135 102 49 47 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2898 3712 1163 Size: 573 1162 814 4307 Fragments arranged by size: 4307 1162 814 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4306 5067 6095 1540 Size: 275 186 2305 761 1028 2301 Fragments arranged by size: 2305 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2237 2361 3906 4917 5002 6073 2237 Size: 124 1545 1011 85 1071 3020 Fragments arranged by size: 3020 1545 1071 1011 124 85 BsmI GAATG_Cn' Cuts at: 4367 4460 4367 Size: 93 6763 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2237 2361 2637 3023 3338 3355 Size: 399 306 141 124 276 386 315 17 Cuts at: 3355 3460 3711 3737 3906 3913 4175 4917 5002 Size: 105 251 26 169 7 262 742 85 Cuts at: 5002 6073 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 386 315 306 276 262 251 169 141 124 105 85 26 17 7 BspEI T'CCGG_A Cuts at: 6519 6519 Size: 6856 BspHI T'CATG_A Cuts at: 2211 2415 4745 6475 2211 Size: 204 2330 1730 2592 Fragments arranged by size: 2592 2330 1730 204 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2992 3261 3966 4211 844 Size: 2148 269 705 245 3489 Fragments arranged by size: 3489 2148 705 269 245 BsrBI CCG'CTC Cuts at: 1394 1823 3400 4743 1394 Size: 429 1577 1343 3507 Fragments arranged by size: 3507 1577 1343 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5351 5525 65 Size: 1183 4103 174 1396 Fragments arranged by size: 4103 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2873 3969 5500 5687 1700 Size: 110 1063 1096 1531 187 2869 Fragments arranged by size: 2869 1531 1096 1063 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6856 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6856 BssSI C'ACGA_G Cuts at: 3546 4916 5928 3546 Size: 1370 1012 4474 Fragments arranged by size: 4474 1370 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4105 794 Size: 3311 3545 BstBI TT'CG_AA Cuts at: 2007 5660 2007 Size: 3653 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6856 BstXI CCAn_nnnn'nTGG Cuts at: 3214 3214 Size: 6856 BstYI r'GATC_y Cuts at: 1972 2706 3674 4079 4186 4553 4938 4955 6407 Size: 734 968 405 107 367 385 17 1452 Cuts at: 6407 6493 6505 6851 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 968 734 405 385 367 346 107 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2133 2469 3839 4040 2133 Size: 336 1370 201 4949 Fragments arranged by size: 4949 1370 336 201 BtgI C'CryG_G Cuts at: 513 1065 2022 3177 3874 513 Size: 552 957 1155 697 3495 Fragments arranged by size: 3495 1155 957 697 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2851 487 Size: 683 56 1625 4492 Fragments arranged by size: 4492 1625 683 56 BtsI GCAGTG_nn' Cuts at: 2724 2886 4372 5166 5186 2724 Size: 162 1486 794 20 4394 Fragments arranged by size: 4394 1486 794 162 20 ClaI AT'CG_AT Cuts at: 1554 2664 1554 Size: 1110 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3573 3608 529 Size: 35 3009 35 3777 Fragments arranged by size: 3777 3009 35 35 DraI TTT'AAA Cuts at: 4160 4160 Size: 6856 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5863 809 Size: 507 4547 1802 Fragments arranged by size: 4547 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2144 3192 4303 Size: 54 1425 50 275 18 314 1048 1111 Cuts at: 4303 5192 6821 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1111 1048 889 314 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4303 1537 Size: 275 2491 4090 Fragments arranged by size: 4090 2491 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3389 3407 4786 1353 Size: 2036 18 1379 3423 Fragments arranged by size: 3423 2036 1379 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5443 5817 5963 387 Size: 1064 138 3854 374 146 1280 Fragments arranged by size: 3854 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2235 2235 Size: 6856 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2130 2254 2930 3236 3373 3403 3491 Size: 393 498 124 676 306 137 30 88 Cuts at: 3491 3968 4919 5516 6303 6571 1239 Size: 477 951 597 787 268 1524 Fragments arranged by size: 1524 951 787 676 597 498 477 393 306 268 137 124 88 30 EcoNI CCTnn'n_nnAGG Cuts at: 3158 3158 Size: 6856 EcoO109I rG'GnC_Cy Cuts at: 2027 2827 3335 3910 2027 Size: 800 508 575 4973 Fragments arranged by size: 4973 800 575 508 EcoRI G'AATT_C Cuts at: 4165 4543 4165 Size: 378 6478 FspI TGC'GCA Cuts at: 5362 5362 Size: 6856 HaeII r_GCGC'y Cuts at: 1678 2260 3223 3885 6003 1678 Size: 582 963 662 2118 2531 Fragments arranged by size: 2531 2118 963 662 582 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2993 3025 4064 4096 5497 Size: 32 135 32 1421 32 1039 32 1401 Cuts at: 5497 5529 5571 5603 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1421 1401 1039 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4200 4446 669 Size: 988 281 2262 246 3079 Fragments arranged by size: 3079 2262 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6856 HpaI GTT'AAC Cuts at: 4446 4446 Size: 6856 KpnI G_GTAC'C Cuts at: 2057 2066 4181 2057 Size: 9 2115 4732 Fragments arranged by size: 4732 2115 9 MfeI C'AATT_G Cuts at: 4103 4455 4103 Size: 352 6504 MluI A'CGCG_T Cuts at: 6732 6732 Size: 6856 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5970 6154 1305 Size: 4665 184 2007 Fragments arranged by size: 4665 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3194 10 Size: 54 1425 343 1362 3672 Fragments arranged by size: 3672 1425 1362 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2580 2600 3299 3548 3899 4814 5173 Size: 648 1414 20 699 249 351 915 359 Cuts at: 5173 5332 518 Size: 159 2042 Fragments arranged by size: 2042 1414 915 699 648 359 351 249 159 20 MspA1I CmG'CkG Cuts at: 1606 2045 2200 3642 4272 4949 6097 6342 1606 Size: 439 155 1442 630 677 1148 245 2120 Fragments arranged by size: 2120 1442 1148 677 630 439 245 155 NcoI C'CATG_G Cuts at: 513 1065 2022 3177 513 Size: 552 957 1155 4192 Fragments arranged by size: 4192 1155 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6856 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6856 NotI GC'GGCC_GC Cuts at: 4303 4303 Size: 6856 NspI r_CATG'y Cuts at: 1132 1229 4214 5759 1132 Size: 97 2985 1545 2229 Fragments arranged by size: 2985 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3023 3105 3464 1348 Size: 1675 82 359 4740 Fragments arranged by size: 4740 1675 359 82 PciI A'CATG_T Cuts at: 5755 5755 Size: 6856 PflMI CCAn_nnn'nTGG Cuts at: 1108 2804 3703 1108 Size: 1696 899 4261 Fragments arranged by size: 4261 1696 899 PfoI T'CCnGG_A Cuts at: 3213 3597 3213 Size: 384 6472 PmeI GTTT'AAAC Cuts at: 4160 4160 Size: 6856 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4925 4957 6438 6470 4925 Size: 32 1481 32 5311 Fragments arranged by size: 5311 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2027 2827 2027 Size: 800 6056 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6856 PsiI TTA'TAA Cuts at: 4426 4426 Size: 6856 PspOMI G'GGCC_C Cuts at: 3334 3909 3334 Size: 575 6281 PstI C_TGCA'G Cuts at: 830 2199 3120 3641 4208 830 Size: 1369 921 521 567 3478 Fragments arranged by size: 3478 1369 921 567 521 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6856 PvuII CAG'CTG Cuts at: 2200 3642 2200 Size: 1442 5414 SacI G_AGCT'C Cuts at: 2237 2237 Size: 6856 SalI G'TCGA_C Cuts at: 1936 4198 1936 Size: 2262 4594 SanDI GG'GwC_CC Cuts at: 2027 2027 Size: 6856 SapI GCTCTTCn'nnn_ Cuts at: 3407 3407 Size: 6856 SbfI CC_TGCA'GG Cuts at: 4208 4208 Size: 6856 ScaI AGT'ACT Cuts at: 5104 6816 5104 Size: 1712 5144 SfcI C'TryA_G Cuts at: 826 1045 2195 3116 3637 4204 5339 6020 6211 Size: 219 1150 921 521 567 1135 681 191 Cuts at: 6211 826 Size: 1471 Fragments arranged by size: 1471 1150 1135 921 681 567 521 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6856 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6856 SmaI CCC'GGG Cuts at: 1872 4183 1872 Size: 2311 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2554 3583 4960 5861 6123 820 Size: 197 926 611 1029 1377 901 262 1553 Fragments arranged by size: 1553 1377 1029 926 901 611 262 197 SnaBI TAC'GTA Cuts at: 493 4153 4647 493 Size: 3660 494 2702 Fragments arranged by size: 3660 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6856 SphI G_CATG'C Cuts at: 1132 4214 1132 Size: 3082 3774 SspI AAT'ATT Cuts at: 5 52 4780 5 Size: 47 4728 2081 Fragments arranged by size: 4728 2081 47 StuI AGG'CCT Cuts at: 1517 2739 3497 1517 Size: 1222 758 4876 Fragments arranged by size: 4876 1222 758 StyI C'CwwG_G Cuts at: 513 1065 2022 2916 2928 3177 4295 513 Size: 552 957 894 12 249 1118 3074 Fragments arranged by size: 3074 1118 957 894 552 249 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2827 2831 3271 3293 3580 3770 4883 Size: 1255 1247 4 440 22 287 190 1113 Cuts at: 4883 4900 5053 5238 5683 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1247 1113 445 440 287 190 185 153 22 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5102 6814 96 Size: 274 80 33 51 1443 3125 1712 138 Fragments arranged by size: 3125 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3749 3781 6816 6848 3749 Size: 32 3035 32 3757 Fragments arranged by size: 3757 3035 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6856 XbaI T'CTAG_A Cuts at: 4192 4192 Size: 6856 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2086 2245 3142 3547 2086 Size: 159 897 405 5395 Fragments arranged by size: 5395 897 405 159 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6856 XmaI C'CCGG_G Cuts at: 1870 4181 1870 Size: 2311 4545 XmnI GAAnn'nnTTC Cuts at: 2258 4985 2258 Size: 2727 4129 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2280 4664 276 Size: 53 83 186 936 746 2384 2468 Fragments arranged by size: 2468 2384 936 746 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI