(Circular) (Six-base) MAPSORT of: pFN21ASDA0865.seq Check: 7833 from: 1 to: 10303 pFN21ASDA0865 10303 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3575 5529 6533 3575 Size: 1954 1004 7345 Fragments arranged by size: 7345 1954 1004 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8113 278 Size: 53 83 186 936 6577 2468 Fragments arranged by size: 6577 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5356 7646 1937 Size: 3419 2290 4594 Fragments arranged by size: 4594 3419 2290 Acc65I G'GTAC_C Cuts at: 7624 7624 Size: 10303 AclI AA'CG_TT Cuts at: 1563 8430 8803 1563 Size: 6867 373 3063 Fragments arranged by size: 6867 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2186 2282 2591 3555 3683 3858 3967 5099 Size: 554 96 309 964 128 175 109 1132 Cuts at: 5099 5510 5619 7308 7556 8366 9750 10018 1632 Size: 411 109 1689 248 810 1384 268 1917 Fragments arranged by size: 1917 1689 1384 1132 964 810 554 411 309 268 248 175 128 109 109 96 AfeI AGC'GCT Cuts at: 3293 3293 Size: 10303 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10106 AflIII A'CryG_T Cuts at: 1203 3613 5193 6444 9202 10179 1203 Size: 2410 1580 1251 2758 977 1327 Fragments arranged by size: 2758 2410 1580 1327 1251 977 AgeI A'CCGG_T Cuts at: 3879 9134 3879 Size: 5255 5048 AhdI GACnn_n'nnGTC Cuts at: 9032 9032 Size: 10303 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10303 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 7583 1184 Size: 6399 3904 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3403 3435 3992 4024 5244 5276 3403 Size: 32 557 32 1220 32 8430 Fragments arranged by size: 8430 1220 557 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 4102 5521 6789 7477 8046 9618 4102 Size: 1419 1268 688 569 1572 4787 Fragments arranged by size: 4787 1572 1419 1268 688 569 ApaI G_GGCC'C Cuts at: 6435 6906 6435 Size: 471 9832 ApaLI G'TGCA_C Cuts at: 3873 5122 8360 9516 3873 Size: 1249 3238 1156 4660 Fragments arranged by size: 4660 3238 1249 1156 ApoI r'AATT_y Cuts at: 1493 2352 3738 4291 4388 4615 4913 7215 7264 Size: 859 1386 553 97 227 298 2302 49 Cuts at: 7264 7612 7838 7990 10099 1493 Size: 348 226 152 2109 1697 Fragments arranged by size: 2302 2109 1697 1386 859 553 348 298 227 226 152 97 49 AseI AT'TA_AT Cuts at: 160 1032 8857 160 Size: 872 7825 1606 Fragments arranged by size: 7825 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10303 AvaI C'yCGr_G Cuts at: 1870 1943 2296 3197 5646 6134 6160 6188 6302 Size: 73 353 901 2449 488 26 28 114 Cuts at: 6302 6355 6761 7063 7628 1870 Size: 53 406 302 565 4545 Fragments arranged by size: 4545 2449 901 565 488 406 353 302 114 73 53 28 26 BamHI G'GATC_C Cuts at: 4883 7633 8000 4883 Size: 2750 367 7186 Fragments arranged by size: 7186 2750 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3578 6155 6220 6859 7498 Size: 325 217 627 1791 2577 65 639 639 Cuts at: 7498 7624 9079 618 Size: 126 1455 1842 Fragments arranged by size: 2577 1842 1791 1455 639 639 627 325 217 126 65 BanII G_rGCy'C Cuts at: 1391 3009 3258 3967 6368 6435 6632 6819 6906 Size: 1618 249 709 2401 67 197 187 87 Cuts at: 6906 7047 7494 7581 7622 1391 Size: 141 447 87 41 4072 Fragments arranged by size: 4072 2401 1618 709 447 249 197 187 141 87 87 67 41 BbeI G_GCGC'C Cuts at: 3582 6159 6224 6863 3582 Size: 2577 65 639 7022 Fragments arranged by size: 7022 2577 639 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2400 928 Size: 971 501 8831 Fragments arranged by size: 8831 971 501 BbvCI CC'TCA_GC Cuts at: 7385 7385 Size: 10303 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8494 8528 1903 Size: 34 6557 34 3678 Fragments arranged by size: 6557 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5246 7292 8195 9405 5246 Size: 2046 903 1210 6144 Fragments arranged by size: 6144 2046 1210 903 BclI T'GATC_A Cuts at: 1548 2779 4845 1548 Size: 1231 2066 7006 Fragments arranged by size: 7006 2066 1231 BfrBI ATG'CAT Cuts at: 2851 3911 2851 Size: 1060 9243 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3587 5826 6164 Size: 107 122 71 631 605 1915 2239 338 Cuts at: 6164 6487 6663 6784 6896 6953 6968 8914 136 Size: 323 176 121 112 57 15 1946 1525 Fragments arranged by size: 2239 1946 1915 1525 631 605 338 323 176 122 121 112 107 71 57 15 BglII A'GATC_T Cuts at: 3309 5238 10298 3309 Size: 1929 5060 3314 Fragments arranged by size: 5060 3314 1929 BlpI GC'TnA_GC Cuts at: 3248 5202 6098 7488 7720 3248 Size: 1954 896 1390 232 5831 Fragments arranged by size: 5831 1954 1390 896 232 Bme1580I G_kGCm'C Cuts at: 1790 3877 5126 6276 6435 6906 8364 9520 1790 Size: 2087 1249 1150 159 471 1458 1156 2573 Fragments arranged by size: 2573 2087 1458 1249 1156 1150 471 159 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10303 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 8034 8992 442 Size: 416 53 83 399 24 6617 958 1753 Fragments arranged by size: 6617 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10303 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4090 4090 Size: 10303 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2468 2487 2676 4079 5246 5351 6584 6608 Size: 1229 19 189 1403 1167 105 1233 24 Cuts at: 6608 6961 7000 7453 8963 1239 Size: 353 39 453 1510 2579 Fragments arranged by size: 2579 1510 1403 1233 1229 1167 453 353 189 105 39 24 19 Bpu10I CC'TnA_GC Cuts at: 2992 7385 2992 Size: 4393 5910 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2982 6584 7173 8428 9293 9591 2982 Size: 3602 589 1255 865 298 3694 Fragments arranged by size: 3694 3602 1255 865 589 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2605 2754 4227 5847 6584 8966 882 Size: 617 1106 149 1473 1620 737 2382 2219 Fragments arranged by size: 2382 2219 1620 1473 1106 737 617 149 BsaAI yAC'GTr Cuts at: 493 1204 5194 7600 8094 493 Size: 711 3990 2406 494 2702 Fragments arranged by size: 3990 2702 2406 711 494 BsaBI GATnn'nnATC Cuts at: 10297 10297 Size: 10303 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2405 2894 3579 6156 Size: 53 83 186 936 872 489 685 2577 Cuts at: 6156 6221 6860 6960 8110 8492 9127 275 Size: 65 639 100 1150 382 635 1451 Fragments arranged by size: 2577 1451 1150 936 872 685 639 635 489 382 186 100 83 65 53 BsaWI w'CCGG_w Cuts at: 3728 3879 8736 9134 9408 9555 9966 3728 Size: 151 4857 398 274 147 411 4065 Fragments arranged by size: 4857 4065 411 398 274 151 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2361 2391 2460 2490 2649 Size: 30 165 30 958 30 69 30 159 Cuts at: 2649 2679 2720 2750 2879 2909 3428 3458 3992 Size: 30 41 30 129 30 519 30 534 Cuts at: 3992 4022 4623 4653 5046 5076 5349 5379 5762 Size: 30 601 30 393 30 273 30 383 Cuts at: 5762 5792 6299 6314 6329 6344 1178 Size: 30 507 15 15 15 5137 Fragments arranged by size: 5137 958 601 534 519 507 393 383 273 165 159 129 69 41 30 30 30 30 30 30 30 30 30 30 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2284 2382 2900 3394 3423 5067 6054 6128 Size: 1098 98 518 494 29 1644 987 74 Cuts at: 6128 6335 7084 7450 1186 Size: 207 749 366 4039 Fragments arranged by size: 4039 1644 1098 987 749 518 494 366 207 98 74 29 BseYI C'CCAG_C Cuts at: 3387 5774 6268 7010 7526 9506 10002 3387 Size: 2387 494 742 516 1980 496 3688 Fragments arranged by size: 3688 2387 1980 742 516 496 494 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5837 6632 7463 1163 Size: 573 4101 795 831 4003 Fragments arranged by size: 4101 4003 831 795 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5799 6491 6718 7753 8514 9542 Size: 275 186 3798 692 227 1035 761 1028 Cuts at: 9542 1540 Size: 2301 Fragments arranged by size: 3798 2301 1035 1028 761 692 275 227 186 BsiHKAI G_wGCw'C Cuts at: 2326 3258 3877 5126 7047 7581 8364 8449 9520 Size: 932 619 1249 1921 534 783 85 1071 Cuts at: 9520 2326 Size: 3109 Fragments arranged by size: 3109 1921 1249 1071 932 783 619 534 85 BsmI GAATG_Cn' Cuts at: 5635 7814 7907 5635 Size: 2179 93 8031 Fragments arranged by size: 8031 2179 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2296 5898 2296 Size: 3602 6701 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2326 3009 3258 3877 3967 5126 6276 Size: 399 536 683 249 619 90 1159 1150 Cuts at: 6276 6368 6435 6632 6819 6906 7047 7494 7581 Size: 92 67 197 187 87 141 447 87 Cuts at: 7581 7622 8364 8449 9520 1391 Size: 41 742 85 1071 2174 Fragments arranged by size: 2174 1159 1150 1071 742 683 619 536 447 399 249 197 187 141 92 90 87 87 85 67 41 BspEI T'CCGG_A Cuts at: 9966 9966 Size: 10303 BspHI T'CATG_A Cuts at: 8192 9922 8192 Size: 1730 8573 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3193 3575 5489 5529 6483 6533 7658 844 Size: 2349 382 1914 40 954 50 1125 3489 Fragments arranged by size: 3489 2349 1914 1125 954 382 50 40 BsrBI CCG'CTC Cuts at: 1394 1823 3183 8190 1394 Size: 429 1360 5007 3507 Fragments arranged by size: 5007 3507 1360 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2343 2415 5140 5640 8798 8972 65 Size: 1183 1095 72 2725 500 3158 174 1396 Fragments arranged by size: 3158 2725 1396 1183 1095 500 174 72 BsrFI r'CCGG_y Cuts at: 1700 1810 3879 5658 6486 6971 8947 9134 1700 Size: 110 2069 1779 828 485 1976 187 2869 Fragments arranged by size: 2869 2069 1976 1779 828 485 187 110 BsrGI T'GTAC_A Cuts at: 96 4254 4477 6255 96 Size: 4158 223 1778 4144 Fragments arranged by size: 4158 4144 1778 223 BssHII G'CGCG_C Cuts at: 1925 6173 6204 6408 6792 1925 Size: 4248 31 204 384 5436 Fragments arranged by size: 5436 4248 384 204 31 BssSI C'ACGA_G Cuts at: 2870 4460 8363 9375 2870 Size: 1590 3903 1012 3798 Fragments arranged by size: 3903 3798 1590 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4511 7477 794 Size: 3717 2966 3620 Fragments arranged by size: 3717 3620 2966 BstBI TT'CG_AA Cuts at: 2007 9107 2007 Size: 7100 3203 BstEII G'GTnAC_C Cuts at: 1184 4517 1184 Size: 3333 6970 BstYI r'GATC_y Cuts at: 1972 3309 4883 5056 5238 6924 7473 7633 8000 Size: 1337 1574 173 182 1686 549 160 367 Cuts at: 8000 8385 8402 9854 9940 9952 10298 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1686 1574 1452 1337 549 385 367 346 182 173 160 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3406 3406 Size: 10303 BtgI C'CryG_G Cuts at: 513 1065 2022 3378 4494 4714 5799 5897 5982 Size: 552 957 1356 1116 220 1085 98 85 Cuts at: 5982 6150 6379 6894 7089 7210 513 Size: 168 229 515 195 121 3606 Fragments arranged by size: 3606 1356 1116 1085 957 552 515 229 220 195 168 121 98 85 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5365 6372 487 Size: 683 56 4139 1007 4418 Fragments arranged by size: 4418 4139 1007 683 56 BtsI GCAGTG_nn' Cuts at: 2588 5378 6206 7819 8613 8633 2588 Size: 2790 828 1613 794 20 4258 Fragments arranged by size: 4258 2790 1613 828 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10303 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10268 DraI TTT'AAA Cuts at: 4180 4912 7607 4180 Size: 732 2695 6876 Fragments arranged by size: 6876 2695 732 DraIII CAC_nnn'GTG Cuts at: 2872 5860 2872 Size: 2988 7315 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2369 9310 809 Size: 507 1053 6941 1802 Fragments arranged by size: 6941 1802 1053 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2062 2818 3787 Size: 54 1425 50 275 18 232 756 969 Cuts at: 3787 4242 5796 5985 5997 6279 6488 6715 6897 Size: 455 1554 189 12 282 209 227 182 Cuts at: 6897 7750 8639 10268 8 Size: 853 889 1629 43 Fragments arranged by size: 1629 1554 1425 969 889 853 756 455 282 275 232 227 209 189 182 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 5796 6488 6715 7750 1537 Size: 275 3984 692 227 1035 4090 Fragments arranged by size: 4090 3984 1035 692 275 227 EarI CTCTTCn'nnn_ Cuts at: 1353 2997 3009 3446 3479 4365 7061 8233 1353 Size: 1644 12 437 33 886 2696 1172 3423 Fragments arranged by size: 3423 2696 1644 1172 886 437 33 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4066 6971 8890 9264 9410 387 Size: 1064 138 2477 2905 1919 374 146 1280 Fragments arranged by size: 2905 2477 1919 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3256 7045 7579 3256 Size: 3789 534 5980 Fragments arranged by size: 5980 3789 534 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 2282 2468 2487 2591 2676 3555 Size: 393 554 96 186 19 104 85 879 Cuts at: 3555 3683 3858 3967 4079 5099 5246 5351 5510 Size: 128 175 109 112 1020 147 105 159 Cuts at: 5510 5619 6584 6608 6961 7000 7308 7453 7556 Size: 109 965 24 353 39 308 145 103 Cuts at: 7556 8366 8963 9750 10018 1239 Size: 810 597 787 268 1524 Fragments arranged by size: 1524 1020 965 879 810 787 597 554 393 353 308 268 186 175 159 147 145 128 112 109 109 105 104 103 96 85 39 24 19 EcoNI CCTnn'n_nnAGG Cuts at: 2264 2483 4064 7561 2264 Size: 219 1581 3497 5006 Fragments arranged by size: 5006 3497 1581 219 EcoO109I rG'GnC_Cy Cuts at: 2304 5221 5929 6126 6431 6432 6786 6903 7551 Size: 2917 708 197 305 1 354 117 648 Cuts at: 7551 2304 Size: 5056 Fragments arranged by size: 5056 2917 708 648 354 305 197 117 1 EcoRI G'AATT_C Cuts at: 4291 7215 7612 7990 4291 Size: 2924 397 378 6604 Fragments arranged by size: 6604 2924 397 378 EcoRV GAT'ATC Cuts at: 6120 6120 Size: 10303 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2267 4814 2267 Size: 2547 7756 FspI TGC'GCA Cuts at: 8809 8809 Size: 10303 HaeII r_GCGC'y Cuts at: 1678 3295 3582 5973 6159 6224 6701 6863 9450 Size: 1617 287 2391 186 65 477 162 2587 Cuts at: 9450 1678 Size: 2531 Fragments arranged by size: 2587 2531 2391 1617 477 287 186 162 65 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2361 2393 2460 2492 2570 Size: 32 135 32 789 32 67 32 78 Cuts at: 2570 2602 2649 2681 2720 2752 2879 2911 2963 Size: 32 47 32 39 32 127 32 52 Cuts at: 2963 2995 3815 3847 3896 3928 5046 5078 5270 Size: 32 820 32 49 32 1118 32 192 Cuts at: 5270 5302 5349 5381 6049 6081 6104 6136 7286 Size: 32 47 32 668 32 23 32 1150 Cuts at: 7286 7318 8944 8976 9018 9050 1373 Size: 32 1626 32 42 32 2626 Fragments arranged by size: 2626 1626 1150 1118 820 789 668 192 135 127 78 67 52 49 47 47 42 39 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 23 HincII GTy'rAC Cuts at: 669 1657 1938 2374 2380 2553 2628 3346 4408 Size: 988 281 436 6 173 75 718 1062 Cuts at: 4408 5357 7647 7893 669 Size: 949 2290 246 3079 Fragments arranged by size: 3079 2290 1062 988 949 718 436 281 246 173 75 6 HindIII A'AGCT_T Cuts at: 748 2603 748 Size: 1855 8448 HpaI GTT'AAC Cuts at: 2628 3346 7893 2628 Size: 718 4547 5038 Fragments arranged by size: 5038 4547 718 KasI G'GCGC_C Cuts at: 3578 6155 6220 6859 3578 Size: 2577 65 639 7022 Fragments arranged by size: 7022 2577 639 65 KpnI G_GTAC'C Cuts at: 7628 7628 Size: 10303 MfeI C'AATT_G Cuts at: 3072 7902 3072 Size: 4830 5473 MluI A'CGCG_T Cuts at: 6444 10179 6444 Size: 3735 6568 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3075 6564 9417 9601 1305 Size: 1770 3489 2853 184 2007 Fragments arranged by size: 3489 2853 2007 1770 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2064 2820 3789 4244 10 Size: 54 1425 343 232 756 969 455 6069 Fragments arranged by size: 6069 1425 969 756 455 343 232 54 MslI CAynn'nnrTG Cuts at: 518 1166 2207 2235 2319 2388 4733 6263 8261 Size: 648 1041 28 84 69 2345 1530 1998 Cuts at: 8261 8620 8779 518 Size: 359 159 2042 Fragments arranged by size: 2345 2042 1998 1530 1041 648 359 159 84 69 28 MspA1I CmG'CkG Cuts at: 1606 3391 3487 5342 5984 6102 6152 6634 7091 Size: 1785 96 1855 642 118 50 482 457 Cuts at: 7091 7384 7719 8396 9544 9789 1606 Size: 293 335 677 1148 245 2120 Fragments arranged by size: 2120 1855 1785 1148 677 642 482 457 335 293 245 118 96 50 NaeI GCC'GGC Cuts at: 6973 6973 Size: 10303 NarI GG'CG_CC Cuts at: 3579 6156 6221 6860 3579 Size: 2577 65 639 7022 Fragments arranged by size: 7022 2577 639 65 NcoI C'CATG_G Cuts at: 513 1065 2022 3378 4494 7210 513 Size: 552 957 1356 1116 2716 3606 Fragments arranged by size: 3606 2716 1356 1116 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10303 NgoMIV G'CCGG_C Cuts at: 6971 6971 Size: 10303 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10303 NotI GC'GGCC_GC Cuts at: 7750 7750 Size: 10303 NsiI A_TGCA'T Cuts at: 2853 3913 2853 Size: 1060 9243 NspI r_CATG'y Cuts at: 1132 1229 2092 2174 2212 3617 3647 4330 5432 Size: 97 863 82 38 1405 30 683 1102 Cuts at: 5432 5719 7661 9206 1132 Size: 287 1942 1545 2229 Fragments arranged by size: 2229 1942 1545 1405 1102 863 683 287 97 82 38 30 PasI CC'CwG_GG Cuts at: 1348 6437 7494 1348 Size: 5089 1057 4157 Fragments arranged by size: 5089 4157 1057 PciI A'CATG_T Cuts at: 3613 9202 3613 Size: 5589 4714 PflMI CCAn_nnn'nTGG Cuts at: 1108 4102 1108 Size: 2994 7309 PfoI T'CCnGG_A Cuts at: 2484 2745 3477 4101 5223 6585 2484 Size: 261 732 624 1122 1362 6202 Fragments arranged by size: 6202 1362 1122 732 624 261 PmeI GTTT'AAAC Cuts at: 7607 7607 Size: 10303 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 8372 8404 9885 9917 3992 Size: 32 4348 32 1481 32 4378 Fragments arranged by size: 4378 4348 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2304 5221 5929 6126 2304 Size: 2917 708 197 6481 Fragments arranged by size: 6481 2917 708 197 PshAI GACnn'nnGTC Cuts at: 1613 4927 6518 1613 Size: 3314 1591 5398 Fragments arranged by size: 5398 3314 1591 PsiI TTA'TAA Cuts at: 5477 7873 5477 Size: 2396 7907 PspOMI G'GGCC_C Cuts at: 6431 6902 6431 Size: 471 9832 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5669 5701 5669 Size: 32 10271 PstI C_TGCA'G Cuts at: 830 3572 5580 6911 7419 7655 830 Size: 2742 2008 1331 508 236 3478 Fragments arranged by size: 3478 2742 2008 1331 508 236 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10303 PvuII CAG'CTG Cuts at: 3391 5342 7384 3391 Size: 1951 2042 6310 Fragments arranged by size: 6310 2042 1951 SacI G_AGCT'C Cuts at: 3258 7047 7581 3258 Size: 3789 534 5980 Fragments arranged by size: 5980 3789 534 SacII CC_GC'GG Cuts at: 5985 6153 7092 5985 Size: 168 939 9196 Fragments arranged by size: 9196 939 168 SalI G'TCGA_C Cuts at: 1936 5355 7645 1936 Size: 3419 2290 4594 Fragments arranged by size: 4594 3419 2290 SanDI GG'GwC_CC Cuts at: 2304 5929 6126 2304 Size: 3625 197 6481 Fragments arranged by size: 6481 3625 197 SapI GCTCTTCn'nnn_ Cuts at: 2997 3479 2997 Size: 482 9821 SbfI CC_TGCA'GG Cuts at: 3572 7419 7655 3572 Size: 3847 236 6220 Fragments arranged by size: 6220 3847 236 ScaI AGT'ACT Cuts at: 2786 6647 8551 10263 2786 Size: 3861 1904 1712 2826 Fragments arranged by size: 3861 2826 1904 1712 SfcI C'TryA_G Cuts at: 826 1045 2213 2520 3568 5456 5576 6040 6781 Size: 219 1168 307 1048 1888 120 464 741 Cuts at: 6781 6907 7000 7415 7651 8786 9467 9658 826 Size: 126 93 415 236 1135 681 191 1471 Fragments arranged by size: 1888 1471 1168 1135 1048 741 681 464 415 307 236 219 191 126 120 93 SfoI GGC'GCC Cuts at: 3580 6157 6222 6861 3580 Size: 2577 65 639 7022 Fragments arranged by size: 7022 2577 639 65 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10303 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10303 SmaI CCC'GGG Cuts at: 1872 5648 6136 6162 6190 6304 6357 6763 7630 Size: 3776 488 26 28 114 53 406 867 Cuts at: 7630 1872 Size: 4545 Fragments arranged by size: 4545 3776 867 488 406 114 53 28 26 SmlI C'TyrA_G Cuts at: 820 1017 1943 2997 6599 7188 8407 9308 9570 Size: 197 926 1054 3602 589 1219 901 262 Cuts at: 9570 820 Size: 1553 Fragments arranged by size: 3602 1553 1219 1054 926 901 589 262 197 SnaBI TAC'GTA Cuts at: 493 7600 8094 493 Size: 7107 494 2702 Fragments arranged by size: 7107 2702 494 SpeI A'CTAG_T Cuts at: 152 4829 152 Size: 4677 5626 SphI G_CATG'C Cuts at: 1132 2092 2174 4330 5432 7661 1132 Size: 960 82 2156 1102 2229 3774 Fragments arranged by size: 3774 2229 2156 1102 960 82 SrfI GCCC'GGGC Cuts at: 6357 6763 6357 Size: 406 9897 SspI AAT'ATT Cuts at: 5 52 4029 4175 4350 8227 5 Size: 47 3977 146 175 3877 2081 Fragments arranged by size: 3977 3877 2081 175 146 47 StuI AGG'CCT Cuts at: 1517 7021 1517 Size: 5504 4799 StyI C'CwwG_G Cuts at: 513 1065 2022 2059 3378 4036 4494 6864 7210 Size: 552 957 37 1319 658 458 2370 346 Cuts at: 7210 7742 513 Size: 532 3074 Fragments arranged by size: 3074 2370 1319 957 658 552 532 458 346 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6888 8330 8347 8500 8685 9130 325 Size: 1255 5308 1442 17 153 185 445 1498 Fragments arranged by size: 5308 1498 1442 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2784 4254 4477 Size: 274 80 33 51 1443 807 1470 223 Cuts at: 4477 4668 6255 6645 8549 10261 96 Size: 191 1587 390 1904 1712 138 Fragments arranged by size: 1904 1712 1587 1470 1443 807 390 274 223 191 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3428 3460 5762 5794 6314 6346 10263 10295 3428 Size: 32 2302 32 520 32 3917 32 3436 Fragments arranged by size: 3917 3436 2302 520 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10303 XbaI T'CTAG_A Cuts at: 4990 7639 4990 Size: 2649 7654 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2492 4556 5822 6007 2492 Size: 2064 1266 185 6788 Fragments arranged by size: 6788 2064 1266 185 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10303 XmaI C'CCGG_G Cuts at: 1870 5646 6134 6160 6188 6302 6355 6761 7628 Size: 3776 488 26 28 114 53 406 867 Cuts at: 7628 1870 Size: 4545 Fragments arranged by size: 4545 3776 867 488 406 114 53 28 26 XmnI GAAnn'nnTTC Cuts at: 2250 4007 4374 5240 8432 2250 Size: 1757 367 866 3192 4121 Fragments arranged by size: 4121 3192 1757 866 367 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8111 276 Size: 53 83 186 936 6577 2468 Fragments arranged by size: 6577 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BsiWI BstXI BstZ17I FseI FspAI NruI PacI PmlI PspXI RsrII SexAI SfiI SwaI