(Circular) (Six-base) MAPSORT of: pFN21ASDA0823.seq Check: 1883 from: 1 to: 6430 pFN21ASDA0823 6430 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2459 2459 Size: 6430 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4240 278 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3773 1937 Size: 1836 4594 Acc65I G'GTAC_C Cuts at: 3751 3751 Size: 6430 AclI AA'CG_TT Cuts at: 1563 4557 4930 1563 Size: 2994 373 3063 Fragments arranged by size: 3063 2994 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2144 2306 3491 4493 5877 6145 1632 Size: 512 162 1185 1002 1384 268 1917 Fragments arranged by size: 1917 1384 1185 1002 512 268 162 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6233 AflIII A'CryG_T Cuts at: 1203 5329 6306 1203 Size: 4126 977 1327 Fragments arranged by size: 4126 1327 977 AgeI A'CCGG_T Cuts at: 5261 5261 Size: 6430 AhdI GACnn_n'nnGTC Cuts at: 5159 5159 Size: 6430 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6430 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2467 2664 2844 3153 3501 1184 Size: 1283 197 180 309 348 4113 Fragments arranged by size: 4113 1283 348 309 197 180 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2924 2956 2924 Size: 32 6398 AlwNI CAG_nnn'CTG Cuts at: 2062 2122 2686 4173 5745 2062 Size: 60 564 1487 1572 2747 Fragments arranged by size: 2747 1572 1487 564 60 ApaI G_GGCC'C Cuts at: 2116 2116 Size: 6430 ApaLI G'TGCA_C Cuts at: 4487 5643 4487 Size: 1156 5274 ApoI r'AATT_y Cuts at: 1493 3251 3739 3965 4117 6226 1493 Size: 1758 488 226 152 2109 1697 Fragments arranged by size: 2109 1758 1697 488 226 152 AseI AT'TA_AT Cuts at: 160 1032 4984 160 Size: 872 3952 1606 Fragments arranged by size: 3952 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 AvaI C'yCGr_G Cuts at: 1870 1943 3755 1870 Size: 73 1812 4545 Fragments arranged by size: 4545 1812 73 BamHI G'GATC_C Cuts at: 3760 4127 3760 Size: 367 6063 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2071 2393 2708 3370 3751 Size: 325 217 627 284 322 315 662 381 Cuts at: 3751 5206 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 662 627 381 325 322 315 284 217 BanII G_rGCy'C Cuts at: 1391 2116 2763 2817 3313 3452 3749 1391 Size: 725 647 54 496 139 297 4072 Fragments arranged by size: 4072 725 647 496 297 139 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5459 BbvCI CC'TCA_GC Cuts at: 2252 2999 3144 2252 Size: 747 145 5538 Fragments arranged by size: 5538 747 145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2486 2520 4621 4655 1903 Size: 34 549 34 2101 34 3678 Fragments arranged by size: 3678 2101 549 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2735 4322 5532 2735 Size: 1587 1210 3633 Fragments arranged by size: 3633 1587 1210 BclI T'GATC_A Cuts at: 1548 2982 1548 Size: 1434 4996 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2231 5041 136 Size: 107 122 71 631 605 559 2810 1525 Fragments arranged by size: 2810 1525 631 605 559 122 107 71 BglII A'GATC_T Cuts at: 6425 6425 Size: 6430 BlpI GC'TnA_GC Cuts at: 2757 3500 3847 2757 Size: 743 347 5340 Fragments arranged by size: 5340 743 347 Bme1580I G_kGCm'C Cuts at: 1790 2076 2116 2137 4491 5647 1790 Size: 286 40 21 2354 1156 2573 Fragments arranged by size: 2573 2354 1156 286 40 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6430 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2809 4161 5119 Size: 416 53 83 399 24 1392 1352 958 Cuts at: 5119 442 Size: 1753 Fragments arranged by size: 1753 1392 1352 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6430 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2900 2900 Size: 6430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2102 2261 2751 2978 3016 3248 3448 Size: 845 18 159 490 227 38 232 200 Cuts at: 3448 3485 5090 1239 Size: 37 1605 2579 Fragments arranged by size: 2579 1605 845 490 232 227 200 159 38 37 18 Bpu10I CC'TnA_GC Cuts at: 2252 2634 2999 3144 3515 2252 Size: 382 365 145 371 5167 Fragments arranged by size: 5167 382 371 365 145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3037 4555 5420 5718 3037 Size: 1518 865 298 3749 Fragments arranged by size: 3749 1518 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2567 3179 5093 882 Size: 617 1068 612 1914 2219 Fragments arranged by size: 2219 1914 1068 617 612 BsaAI yAC'GTr Cuts at: 493 1204 2035 3727 4221 493 Size: 711 831 1692 494 2702 Fragments arranged by size: 2702 1692 831 711 494 BsaBI GATnn'nnATC Cuts at: 6424 6424 Size: 6430 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2262 3414 4237 4619 Size: 53 83 186 936 729 1152 823 382 Cuts at: 4619 5254 275 Size: 635 1451 Fragments arranged by size: 1451 1152 936 823 729 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3313 4863 5261 5535 5682 6093 3313 Size: 1550 398 274 147 411 3650 Fragments arranged by size: 3650 1550 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2769 2799 2924 2954 1178 Size: 30 165 30 1366 30 125 30 4654 Fragments arranged by size: 4654 1366 165 125 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2372 2753 2945 3698 3707 1186 Size: 1186 381 192 753 9 3909 Fragments arranged by size: 3909 1186 753 381 192 9 BseYI C'CCAG_C Cuts at: 2115 2808 5633 6129 2115 Size: 693 2825 496 2416 Fragments arranged by size: 2825 2416 693 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2705 3159 1163 Size: 573 969 454 4434 Fragments arranged by size: 4434 969 573 454 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2200 3880 4641 5669 1540 Size: 275 186 199 1680 761 1028 2301 Fragments arranged by size: 2301 1680 1028 761 275 199 186 BsiHKAI G_wGCw'C Cuts at: 2763 4491 4576 5647 2763 Size: 1728 85 1071 3546 Fragments arranged by size: 3546 1728 1071 85 BsmI GAATG_Cn' Cuts at: 3941 4034 3941 Size: 93 6337 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2116 2137 2763 2817 3313 3452 Size: 399 286 40 21 626 54 496 139 Cuts at: 3452 3749 4491 4576 5647 1391 Size: 297 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 626 496 399 297 286 139 85 54 40 21 BspEI T'CCGG_A Cuts at: 6093 6093 Size: 6430 BspHI T'CATG_A Cuts at: 2985 4319 6049 2985 Size: 1334 1730 3366 Fragments arranged by size: 3366 1730 1334 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2049 2459 2585 2754 3785 844 Size: 1205 410 126 169 1031 3489 Fragments arranged by size: 3489 1205 1031 410 169 126 BsrBI CCG'CTC Cuts at: 1394 1823 2086 2179 4317 1394 Size: 429 263 93 2138 3507 Fragments arranged by size: 3507 2138 429 263 93 BsrDI GCAATG_nn' Cuts at: 65 1248 2320 3619 4925 5099 65 Size: 1183 1072 1299 1306 174 1396 Fragments arranged by size: 1396 1306 1299 1183 1072 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5074 5261 1700 Size: 110 3264 187 2869 Fragments arranged by size: 3264 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6430 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6430 BssSI C'ACGA_G Cuts at: 3270 4490 5502 3270 Size: 1220 1012 4198 Fragments arranged by size: 4198 1220 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2152 3370 794 Size: 1358 1218 3854 Fragments arranged by size: 3854 1358 1218 BstBI TT'CG_AA Cuts at: 2007 5234 2007 Size: 3227 3203 BstEII G'GTnAC_C Cuts at: 1184 3637 1184 Size: 2453 3977 BstXI CCAn_nnnn'nTGG Cuts at: 2035 2035 Size: 6430 BstYI r'GATC_y Cuts at: 1972 2475 3264 3649 3760 4127 4512 4529 5981 Size: 503 789 385 111 367 385 17 1452 Cuts at: 5981 6067 6079 6425 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 789 503 385 385 367 346 111 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2389 2773 2872 2901 3046 513 Size: 552 957 367 384 99 29 145 3897 Fragments arranged by size: 3897 957 552 384 367 145 99 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5691 Fragments arranged by size: 5691 683 56 BtsI GCAGTG_nn' Cuts at: 2659 2714 3568 3946 4740 4760 2659 Size: 55 854 378 794 20 4329 Fragments arranged by size: 4329 854 794 378 55 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6430 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6395 DraI TTT'AAA Cuts at: 3734 3734 Size: 6430 DraIII CAC_nnn'GTG Cuts at: 3275 3275 Size: 6430 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5437 809 Size: 507 4121 1802 Fragments arranged by size: 4121 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2197 2455 Size: 54 1425 50 275 18 195 172 258 Cuts at: 2455 3339 3408 3540 3877 4766 6395 8 Size: 884 69 132 337 889 1629 43 Fragments arranged by size: 1629 1425 889 884 337 275 258 195 172 132 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2197 3877 1537 Size: 275 385 1680 4090 Fragments arranged by size: 4090 1680 385 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2931 4360 1353 Size: 1578 1429 3423 Fragments arranged by size: 3423 1578 1429 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5017 5391 5537 387 Size: 1064 138 3428 374 146 1280 Fragments arranged by size: 3428 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2761 2761 Size: 6430 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2102 2144 2261 2306 2751 2978 Size: 393 452 18 42 117 45 445 227 Cuts at: 2978 3016 3248 3448 3485 3491 4493 5090 5877 Size: 38 232 200 37 6 1002 597 787 Cuts at: 5877 6145 1239 Size: 268 1524 Fragments arranged by size: 1524 1002 787 597 452 445 393 268 232 227 200 117 45 42 38 37 18 6 EcoNI CCTnn'n_nnAGG Cuts at: 2240 2251 2543 2927 2240 Size: 11 292 384 5743 Fragments arranged by size: 5743 384 292 11 EcoO109I rG'GnC_Cy Cuts at: 2683 2947 3027 3211 3574 3618 3673 2683 Size: 264 80 184 363 44 55 5440 Fragments arranged by size: 5440 363 264 184 80 55 44 EcoRI G'AATT_C Cuts at: 3739 4117 3739 Size: 378 6052 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3031 3595 3031 Size: 564 5866 FspI TGC'GCA Cuts at: 4936 4936 Size: 6430 HaeII r_GCGC'y Cuts at: 1678 2110 5577 1678 Size: 432 3467 2531 Fragments arranged by size: 3467 2531 432 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3105 3137 5071 5103 5145 Size: 32 135 32 1533 32 1934 32 42 Cuts at: 5145 5177 1373 Size: 32 2626 Fragments arranged by size: 2626 1934 1533 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2512 3774 4020 669 Size: 988 281 574 1262 246 3079 Fragments arranged by size: 3079 1262 988 574 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6430 HpaI GTT'AAC Cuts at: 4020 4020 Size: 6430 KpnI G_GTAC'C Cuts at: 3755 3755 Size: 6430 MfeI C'AATT_G Cuts at: 4029 4029 Size: 6430 MluI A'CGCG_T Cuts at: 6306 6306 Size: 6430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3057 5544 5728 1305 Size: 1752 2487 184 2007 Fragments arranged by size: 2487 2007 1752 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 3341 3542 10 Size: 54 1425 343 195 1314 201 2898 Fragments arranged by size: 2898 1425 1314 343 201 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2973 4388 4747 4906 518 Size: 648 1807 1415 359 159 2042 Fragments arranged by size: 2042 1807 1415 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2059 2122 2675 2756 2828 2998 3133 3143 Size: 453 63 553 81 72 170 135 10 Cuts at: 3143 3154 3481 3645 3846 4523 5671 5916 1606 Size: 11 327 164 201 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 553 453 327 245 201 170 164 135 81 72 63 11 10 NcoI C'CATG_G Cuts at: 513 1065 2022 2389 2773 2872 2901 513 Size: 552 957 367 384 99 29 4042 Fragments arranged by size: 4042 957 552 384 367 99 29 NdeI CA'TA_TG Cuts at: 387 2533 387 Size: 2146 4284 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6430 NotI GC'GGCC_GC Cuts at: 2197 3877 2197 Size: 1680 4750 NspI r_CATG'y Cuts at: 1132 1229 2530 2825 3289 3788 5333 1132 Size: 97 1301 295 464 499 1545 2229 Fragments arranged by size: 2229 1545 1301 499 464 295 97 PasI CC'CwG_GG Cuts at: 1348 2704 1348 Size: 1356 5074 PciI A'CATG_T Cuts at: 5329 5329 Size: 6430 PflMI CCAn_nnn'nTGG Cuts at: 1108 2686 3126 1108 Size: 1578 440 4412 Fragments arranged by size: 4412 1578 440 PfoI T'CCnGG_A Cuts at: 2766 2766 Size: 6430 PmeI GTTT'AAAC Cuts at: 3734 3734 Size: 6430 PmlI CAC'GTG Cuts at: 2035 2035 Size: 6430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2924 2956 4499 4531 6012 6044 2924 Size: 32 1543 32 1481 32 3310 Fragments arranged by size: 3310 1543 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2683 2947 3027 3211 3618 2683 Size: 264 80 184 407 5495 Fragments arranged by size: 5495 407 264 184 80 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6430 PsiI TTA'TAA Cuts at: 4000 4000 Size: 6430 PspOMI G'GGCC_C Cuts at: 2112 2112 Size: 6430 PstI C_TGCA'G Cuts at: 830 2058 2446 3490 3782 830 Size: 1228 388 1044 292 3478 Fragments arranged by size: 3478 1228 1044 388 292 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6430 PvuII CAG'CTG Cuts at: 2059 2122 2756 2828 2998 3143 2059 Size: 63 634 72 170 145 5346 Fragments arranged by size: 5346 634 170 145 72 63 SacI G_AGCT'C Cuts at: 2763 2763 Size: 6430 SalI G'TCGA_C Cuts at: 1936 3772 1936 Size: 1836 4594 SbfI CC_TGCA'GG Cuts at: 3782 3782 Size: 6430 ScaI AGT'ACT Cuts at: 4678 6390 4678 Size: 1712 4718 SexAI A'CCwGG_T Cuts at: 2466 2466 Size: 6430 SfcI C'TryA_G Cuts at: 826 1045 2054 2442 3379 3486 3778 4913 5594 Size: 219 1009 388 937 107 292 1135 681 Cuts at: 5594 5785 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1009 937 681 388 292 219 191 107 SfiI GGCCn_nnn'nGGCC Cuts at: 2231 2231 Size: 6430 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6430 SmaI CCC'GGG Cuts at: 1872 3757 1872 Size: 1885 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3016 4534 5435 5697 820 Size: 197 926 1073 1518 901 262 1553 Fragments arranged by size: 1553 1518 1073 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3727 4221 493 Size: 3234 494 2702 Fragments arranged by size: 3234 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6430 SphI G_CATG'C Cuts at: 1132 2825 3788 1132 Size: 1693 963 3774 Fragments arranged by size: 3774 1693 963 SspI AAT'ATT Cuts at: 5 52 4354 5 Size: 47 4302 2081 Fragments arranged by size: 4302 2081 47 StuI AGG'CCT Cuts at: 1517 2246 1517 Size: 729 5701 StyI C'CwwG_G Cuts at: 513 1065 2022 2389 2409 2773 2872 2901 3869 Size: 552 957 367 20 364 99 29 968 Cuts at: 3869 513 Size: 3074 Fragments arranged by size: 3074 968 957 552 367 364 99 29 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2615 4457 4474 4627 4812 5257 325 Size: 1255 1035 1842 17 153 185 445 1498 Fragments arranged by size: 1842 1498 1255 1035 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4676 6388 96 Size: 274 80 33 51 1443 2699 1712 138 Fragments arranged by size: 2699 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2769 2801 2996 3028 6390 6422 2769 Size: 32 195 32 3362 32 2777 Fragments arranged by size: 3362 2777 195 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6430 XbaI T'CTAG_A Cuts at: 3766 3766 Size: 6430 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3685 3685 Size: 6430 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6430 XmaI C'CCGG_G Cuts at: 1870 3755 1870 Size: 1885 4545 XmnI GAAnn'nnTTC Cuts at: 4559 4559 Size: 6430 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4238 276 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SfoI SrfI SwaI