(Circular) (Six-base) MAPSORT of: pFN21ASDA0704.seq Check: 9636 from: 1 to: 7390 pFN21ASDA0704 7390 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2757 5200 278 Size: 53 83 186 936 1221 2443 2468 Fragments arranged by size: 2468 2443 1221 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2113 3343 4733 1937 Size: 176 1230 1390 4594 Fragments arranged by size: 4594 1390 1230 176 Acc65I G'GTAC_C Cuts at: 4711 4711 Size: 7390 AclI AA'CG_TT Cuts at: 1563 5517 5890 1563 Size: 3954 373 3063 Fragments arranged by size: 3954 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2420 2652 3125 3182 3396 5453 6837 7105 Size: 788 232 473 57 214 2057 1384 268 Cuts at: 7105 1632 Size: 1917 Fragments arranged by size: 2057 1917 1384 788 473 268 232 214 57 AflII C'TTAA_G Cuts at: 820 1017 4102 820 Size: 197 3085 4108 Fragments arranged by size: 4108 3085 197 AflIII A'CryG_T Cuts at: 1203 6289 7266 1203 Size: 5086 977 1327 Fragments arranged by size: 5086 1327 977 AgeI A'CCGG_T Cuts at: 6221 6221 Size: 7390 AhdI GACnn_n'nnGTC Cuts at: 6119 6119 Size: 7390 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7390 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2727 1184 Size: 1543 5847 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4245 4277 4245 Size: 32 7358 AlwNI CAG_nnn'CTG Cuts at: 2740 5133 6705 2740 Size: 2393 1572 3425 Fragments arranged by size: 3425 2393 1572 ApaLI G'TGCA_C Cuts at: 5447 6603 5447 Size: 1156 6234 ApoI r'AATT_y Cuts at: 1493 2593 3952 3989 4183 4699 4925 5077 7186 Size: 1100 1359 37 194 516 226 152 2109 Cuts at: 7186 1493 Size: 1697 Fragments arranged by size: 2109 1697 1359 1100 516 226 194 152 37 AseI AT'TA_AT Cuts at: 160 1032 5944 160 Size: 872 4912 1606 Fragments arranged by size: 4912 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7390 AvaI C'yCGr_G Cuts at: 1870 1943 3583 4715 1870 Size: 73 1640 1132 4545 Fragments arranged by size: 4545 1640 1132 73 BamHI G'GATC_C Cuts at: 4408 4720 5087 4408 Size: 312 367 6711 Fragments arranged by size: 6711 367 312 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2776 3574 4622 4711 6166 Size: 325 217 627 989 798 1048 89 1455 Cuts at: 6166 618 Size: 1842 Fragments arranged by size: 1842 1455 1048 989 798 627 325 217 89 BanII G_rGCy'C Cuts at: 1391 2331 3316 3678 3726 4709 1391 Size: 940 985 362 48 983 4072 Fragments arranged by size: 4072 985 983 940 362 48 BbeI G_GCGC'C Cuts at: 3578 3578 Size: 7390 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2658 928 Size: 971 759 5660 Fragments arranged by size: 5660 971 759 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2093 2127 2305 2339 4559 4593 5581 Size: 34 156 34 178 34 2220 34 988 Cuts at: 5581 5615 1903 Size: 34 3678 Fragments arranged by size: 3678 2220 988 178 156 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5282 6492 5282 Size: 1210 6180 BclI T'GATC_A Cuts at: 1548 1548 Size: 7390 BfrBI ATG'CAT Cuts at: 2364 3803 2364 Size: 1439 5951 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6001 136 Size: 107 122 71 631 605 4329 1525 Fragments arranged by size: 4329 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3025 3199 4645 7385 3025 Size: 174 1446 2740 3030 Fragments arranged by size: 3030 2740 1446 174 BlpI GC'TnA_GC Cuts at: 4807 4807 Size: 7390 Bme1580I G_kGCm'C Cuts at: 1790 5451 6607 1790 Size: 3661 1156 2573 Fragments arranged by size: 3661 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7390 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2909 5121 6079 Size: 416 53 83 399 24 1492 2212 958 Cuts at: 6079 442 Size: 1753 Fragments arranged by size: 2212 1753 1492 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7390 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3310 3310 Size: 7390 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3280 3731 4225 6050 1239 Size: 2041 451 494 1825 2579 Fragments arranged by size: 2579 2041 1825 494 451 Bpu10I CC'TnA_GC Cuts at: 3147 3384 3147 Size: 237 7153 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5515 6380 6678 5515 Size: 865 298 6227 Fragments arranged by size: 6227 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6053 882 Size: 617 4554 2219 Fragments arranged by size: 4554 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4687 5181 493 Size: 711 3483 494 2702 Fragments arranged by size: 3483 2702 711 494 BsaBI GATnn'nnATC Cuts at: 4142 7384 4142 Size: 3242 4148 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2754 3575 5197 5579 Size: 53 83 186 936 1221 821 1622 382 Cuts at: 5579 6214 275 Size: 635 1451 Fragments arranged by size: 1622 1451 1221 936 821 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3841 5823 6221 6495 6642 7053 3841 Size: 1982 398 274 147 411 4178 Fragments arranged by size: 4178 1982 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2071 2101 3698 3728 4125 Size: 30 165 30 668 30 1597 30 397 Cuts at: 4125 4155 1178 Size: 30 4413 Fragments arranged by size: 4413 1597 668 397 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2396 2920 3121 3124 3233 3719 4303 1186 Size: 1210 524 201 3 109 486 584 4273 Fragments arranged by size: 4273 1210 584 524 486 201 109 3 BseYI C'CCAG_C Cuts at: 2124 3749 6593 7089 2124 Size: 1625 2844 496 2425 Fragments arranged by size: 2844 2425 1625 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6817 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4840 5601 6629 1540 Size: 275 186 2839 761 1028 2301 Fragments arranged by size: 2839 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2084 2388 3316 3726 4128 4211 4572 5451 5536 Size: 304 928 410 402 83 361 879 85 Cuts at: 5536 6607 2084 Size: 1071 2867 Fragments arranged by size: 2867 1071 928 879 410 402 361 304 85 83 BsmI GAATG_Cn' Cuts at: 4901 4994 4901 Size: 93 7297 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 2331 2388 3316 3678 3726 4128 Size: 399 294 247 57 928 362 48 402 Cuts at: 4128 4211 4572 4709 5451 5536 6607 1391 Size: 83 361 137 742 85 1071 2174 Fragments arranged by size: 2174 1071 928 742 402 399 362 361 294 247 137 85 83 57 48 BspEI T'CCGG_A Cuts at: 7053 7053 Size: 7390 BspHI T'CATG_A Cuts at: 5279 7009 5279 Size: 1730 5660 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2881 4161 4745 844 Size: 2037 1280 584 3489 Fragments arranged by size: 3489 2037 1280 584 BsrBI CCG'CTC Cuts at: 1394 1823 5277 1394 Size: 429 3454 3507 Fragments arranged by size: 3507 3454 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2620 5885 6059 65 Size: 1183 1372 3265 174 1396 Fragments arranged by size: 3265 1396 1372 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3571 3658 6034 6221 1700 Size: 110 1761 87 2376 187 2869 Fragments arranged by size: 2869 2376 1761 187 110 87 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7390 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7390 BssSI C'ACGA_G Cuts at: 2643 5450 6462 2643 Size: 2807 1012 3571 Fragments arranged by size: 3571 2807 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3785 794 Size: 2991 4399 BstBI TT'CG_AA Cuts at: 2007 6194 2007 Size: 4187 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7390 BstXI CCAn_nnnn'nTGG Cuts at: 2698 3661 3757 2698 Size: 963 96 6331 Fragments arranged by size: 6331 963 96 BstYI r'GATC_y Cuts at: 1972 2804 3025 3199 4408 4645 4720 5087 5472 Size: 832 221 174 1209 237 75 367 385 Cuts at: 5472 5489 6941 7027 7039 7385 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1209 832 385 367 346 237 221 174 86 75 17 12 Bsu36I CC'TnA_GG Cuts at: 3617 3617 Size: 7390 BtgI C'CryG_G Cuts at: 513 1065 4005 513 Size: 552 2940 3898 Fragments arranged by size: 3898 2940 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6651 Fragments arranged by size: 6651 683 56 BtsI GCAGTG_nn' Cuts at: 2885 4906 5700 5720 2885 Size: 2021 794 20 4555 Fragments arranged by size: 4555 2021 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7390 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7355 DraI TTT'AAA Cuts at: 4694 4694 Size: 7390 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3443 4673 6397 809 Size: 507 2127 1230 1724 1802 Fragments arranged by size: 2127 1802 1724 1230 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3651 4837 5726 Size: 54 1425 50 275 18 1821 1186 889 Cuts at: 5726 7355 8 Size: 1629 43 Fragments arranged by size: 1821 1629 1425 1186 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4837 1537 Size: 275 3025 4090 Fragments arranged by size: 4090 3025 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2101 2539 2961 3415 4321 5320 1353 Size: 748 438 422 454 906 999 3423 Fragments arranged by size: 3423 999 906 748 454 438 422 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3169 3909 5977 6351 6497 387 Size: 1064 138 1580 740 2068 374 146 1280 Fragments arranged by size: 2068 1580 1280 1064 740 374 146 138 EcoICRI GAG'CTC Cuts at: 3314 3724 3314 Size: 410 6980 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2420 2652 3125 3182 3280 3396 3731 Size: 393 788 232 473 57 98 116 335 Cuts at: 3731 4225 5453 6050 6837 7105 1239 Size: 494 1228 597 787 268 1524 Fragments arranged by size: 1524 1228 788 787 597 494 473 393 335 268 232 116 98 57 EcoNI CCTnn'n_nnAGG Cuts at: 3644 3731 3644 Size: 87 7303 EcoO109I rG'GnC_Cy Cuts at: 2893 3518 3637 2893 Size: 625 119 6646 Fragments arranged by size: 6646 625 119 EcoRI G'AATT_C Cuts at: 4699 5077 4699 Size: 378 7012 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2233 2257 3087 2233 Size: 24 830 6536 Fragments arranged by size: 6536 830 24 FspI TGC'GCA Cuts at: 5896 5896 Size: 7390 HaeII r_GCGC'y Cuts at: 1678 3578 6537 1678 Size: 1900 2959 2531 Fragments arranged by size: 2959 2531 1900 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2420 2452 2522 2554 3116 Size: 32 135 32 848 32 70 32 562 Cuts at: 3116 3148 3336 3368 3422 3454 4125 4157 4324 Size: 32 188 32 54 32 671 32 167 Cuts at: 4324 4356 4400 4432 4589 4621 6031 6063 6105 Size: 32 44 32 157 32 1410 32 42 Cuts at: 6105 6137 1373 Size: 32 2626 Fragments arranged by size: 2626 1410 848 671 562 188 167 157 135 70 54 44 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2114 2500 2949 4734 4980 669 Size: 988 281 176 386 449 1785 246 3079 Fragments arranged by size: 3079 1785 988 449 386 281 246 176 HindIII A'AGCT_T Cuts at: 748 748 Size: 7390 HpaI GTT'AAC Cuts at: 2500 4980 2500 Size: 2480 4910 KasI G'GCGC_C Cuts at: 3574 3574 Size: 7390 KpnI G_GTAC'C Cuts at: 4715 4715 Size: 7390 MfeI C'AATT_G Cuts at: 4020 4989 4020 Size: 969 6421 MluI A'CGCG_T Cuts at: 7266 7266 Size: 7390 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4620 6504 6688 1305 Size: 3315 1884 184 2007 Fragments arranged by size: 3315 2007 1884 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3653 10 Size: 54 1425 343 1821 3747 Fragments arranged by size: 3747 1821 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2359 2709 3659 3862 5348 5707 5866 Size: 648 1193 350 950 203 1486 359 159 Cuts at: 5866 518 Size: 2042 Fragments arranged by size: 2042 1486 1193 950 648 359 350 203 159 MspA1I CmG'CkG Cuts at: 1606 2124 3089 3112 3257 4806 5483 6631 6876 Size: 518 965 23 145 1549 677 1148 245 Cuts at: 6876 1606 Size: 2120 Fragments arranged by size: 2120 1549 1148 965 677 518 245 145 23 NaeI GCC'GGC Cuts at: 3573 3573 Size: 7390 NarI GG'CG_CC Cuts at: 3575 3575 Size: 7390 NcoI C'CATG_G Cuts at: 513 1065 4005 513 Size: 552 2940 3898 Fragments arranged by size: 3898 2940 552 NdeI CA'TA_TG Cuts at: 387 3442 3859 387 Size: 3055 417 3918 Fragments arranged by size: 3918 3055 417 NgoMIV G'CCGG_C Cuts at: 3571 3571 Size: 7390 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7390 NotI GC'GGCC_GC Cuts at: 4837 4837 Size: 7390 NsiI A_TGCA'T Cuts at: 2366 3805 2366 Size: 1439 5951 NspI r_CATG'y Cuts at: 1132 1229 4748 6293 1132 Size: 97 3519 1545 2229 Fragments arranged by size: 3519 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7390 PciI A'CATG_T Cuts at: 6289 6289 Size: 7390 PflMI CCAn_nnn'nTGG Cuts at: 1108 2062 4052 1108 Size: 954 1990 4446 Fragments arranged by size: 4446 1990 954 PfoI T'CCnGG_A Cuts at: 3551 3726 3551 Size: 175 7215 PmeI GTTT'AAAC Cuts at: 4694 4694 Size: 7390 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3303 3335 5459 5491 6972 7004 3303 Size: 32 2124 32 1481 32 3689 Fragments arranged by size: 3689 2124 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2893 3637 2893 Size: 744 6646 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7390 PsiI TTA'TAA Cuts at: 4190 4960 4190 Size: 770 6620 PstI C_TGCA'G Cuts at: 830 2892 3693 4530 4742 830 Size: 2062 801 837 212 3478 Fragments arranged by size: 3478 2062 837 801 212 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7390 PvuII CAG'CTG Cuts at: 2124 3112 2124 Size: 988 6402 SacI G_AGCT'C Cuts at: 3316 3726 3316 Size: 410 6980 SalI G'TCGA_C Cuts at: 1936 2112 4732 1936 Size: 176 2620 4594 Fragments arranged by size: 4594 2620 176 SapI GCTCTTCn'nnn_ Cuts at: 2101 3415 2101 Size: 1314 6076 SbfI CC_TGCA'GG Cuts at: 4742 4742 Size: 7390 ScaI AGT'ACT Cuts at: 5638 7350 5638 Size: 1712 5678 SexAI A'CCwGG_T Cuts at: 2718 2718 Size: 7390 SfcI C'TryA_G Cuts at: 826 1045 2888 3689 4369 4526 4738 5873 6554 Size: 219 1843 801 680 157 212 1135 681 Cuts at: 6554 6745 826 Size: 191 1471 Fragments arranged by size: 1843 1471 1135 801 681 680 219 212 191 157 SfoI GGC'GCC Cuts at: 3576 3576 Size: 7390 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7390 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7390 SmaI CCC'GGG Cuts at: 1872 4717 1872 Size: 2845 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4102 5494 6395 6657 820 Size: 197 926 2159 1392 901 262 1553 Fragments arranged by size: 2159 1553 1392 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4687 5181 493 Size: 4194 494 2702 Fragments arranged by size: 4194 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7390 SphI G_CATG'C Cuts at: 1132 4748 1132 Size: 3616 3774 SspI AAT'ATT Cuts at: 5 52 5314 5 Size: 47 5262 2081 Fragments arranged by size: 5262 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7390 StyI C'CwwG_G Cuts at: 513 1065 2044 3513 3645 4005 4829 513 Size: 552 979 1469 132 360 824 3074 Fragments arranged by size: 3074 1469 979 824 552 360 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2180 4600 5417 5434 5587 5772 6217 Size: 1255 600 2420 817 17 153 185 445 Cuts at: 6217 325 Size: 1498 Fragments arranged by size: 2420 1498 1255 817 600 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3714 5636 7348 Size: 274 80 33 51 1443 1737 1922 1712 Cuts at: 7348 96 Size: 138 Fragments arranged by size: 1922 1737 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2877 2909 7350 7382 2877 Size: 32 4441 32 2885 Fragments arranged by size: 4441 2885 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7390 XbaI T'CTAG_A Cuts at: 3947 4471 4726 3947 Size: 524 255 6611 Fragments arranged by size: 6611 524 255 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7390 XmaI C'CCGG_G Cuts at: 1870 4715 1870 Size: 2845 4545 XmnI GAAnn'nnTTC Cuts at: 3867 4014 5519 3867 Size: 147 1505 5738 Fragments arranged by size: 5738 1505 147 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2755 5198 276 Size: 53 83 186 936 1221 2443 2468 Fragments arranged by size: 2468 2443 1221 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI XcmI