Restriction map for
pFN21ASDA0642
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AccII AclI AflII AflIII AgeI
Aor51HI AscI AseI AsnI AviII
BalI BamHI BbrPI BbvCI BcgI
BciVI BfrI BglII BlpI Bpu1102I
BsaAI BsiEI BsiWI Bsp1407I BspDI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I CelII
ClaI CpoI DraI DraIII DsaI
EagI EclXI Eco47III Eco52I FseI
FspI HincII HindII HpaI KspI
MfeI MluI MluNI MscI MunI
MvnI NaeI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 818 GACGTC GACGT^C
AccI 1313 GTCTAC GT^[AC][GT]AC
AccIII 2780 TCCGGA T^CCGGA
AhdI 1216 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1577 GTGCAC G^TGCAC
AocI 1684 CCTCAGG CC^T[ACGT]AGG
ApaI 2612 GGGCCC GGGCC^C
ApaLI 1577 GTGCAC G^TGCAC
AspEI 1216 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3241 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2615 CCCGGG C^[CT]CG[GA]G
AvrII 3325 CCTAGG C^CTAGG
BbeI 2957 GGCGCC GGCGC^C
BclI 2076 TGATCA T^GATCA
BglI 33 GCCTGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3325 CCTAGG C^CTAGG
BsaBI 1595 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2780 TCCGGA [AT]^CCGG[AT]
BseAI 2780 TCCGGA T^CCGGA
BsmBI 3943 GAGACG CGTCTC(1/5)
BsoBI 2615 CCCGGG C^[CT]CG[GA]G
BspEI 2780 TCCGGA T^CCGGA
BspHI 1811 TCATGA T^CATGA
BstEII 865 GGTTACC G^GT[ACGT]ACC
BstPI 865 GGTTACC G^GT[ACGT]ACC
BstXI 1794 CCAACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1684 CCTCAGG CC^T[ACGT]AGG
DrdI 764 GACCTCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1450 CGGCCA [CT]^GGCC[GA]
Eam1105I 1216 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1684 CCTCAGG CC^T[ACGT]AGG
EcoNI 210 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 865 GGTTACC G^GT[ACGT]ACC
EcoRV 1807 GATATC GAT^ATC
FbaI 2076 TGATCA T^GATCA
KasI 2957 GGCGCC G^GCGCC
MamI 1595 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2780 TCCGGA T^CCGGA
MslI 1812 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2957 GGCGCC GG^CGCC
NdeI 1864 CATATG CA^TATG
PflFI 3241 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2119 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1811 TCATGA T^CATGA
SexAI 1200 ACCAGGT A^CC[AT]GGT
SfoI 2957 GGCGCC GGC^GCC
SmaI 2615 CCCGGG CCC^GGG
SmlI 3637 CTTGAG C^T[CT][GA]AG
Tsp45I 494 GTGAC ^GT[GC]AC
Tth111I 3241 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2119 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2888 CCACGCTGTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2615 CCCGGG C^CCGGG
XmaI 2615 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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Acc65I 243 GGTACC G^GTACC
3052 GGTACC
AcyI 818 GACGTC G[GA]^CG[CT]C
2957 GGCGCC
Asp718I 243 GGTACC G^GTACC
3052 GGTACC
BcnI 2615 CCCGG CC^[GC]GG
2616 CCGGG
BsaHI 818 GACGTC G[GA]^CG[CT]C
2957 GGCGCC
BsaI 600 GAGACC GGTCTC(1/5)
1522 GGTCTC
BsgI 3172 CTGCAC GTGCAG(16/14)
3835 GTGCAG
BsmI 979 GCATTC GAATGC(1/-1)
3162 GCATTC
BspMI 1934 ACCTGC ACCTGC(4/8)
3170 ACCTGC
BsrDI 95 CATTGC GCAATG(2/0)
1657 GCAATG
BsrFI 2654 ACCGGC [GA]^CCGG[CT]
3389 ACCGGC
BstAPI 1929 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3681 GCAGCAGCTGC
Cfr10I 2654 ACCGGC [GA]^CCGG[CT]
3389 ACCGGC
EcoRI 3189 GAATTC G^AATTC
3330 GAATTC
HaeII 2957 GGCGCC [GA]GCGC^[CT]
3641 AGCGCC
Hin1I 818 GACGTC G[GA]^CG[CT]C
2957 GGCGCC
HindIII 1788 AAGCTT A^AGCTT
2827 AAGCTT
KpnI 243 GGTACC GGTAC^C
3052 GGTACC
NciI 2615 CCCGG CC^[GC]GG
2616 CCGGG
NspI 554 GCATGT [GA]CATG^[CT]
2872 GCATGT
PpuMI 1701 AGGTCCC [GA]G^G[AT]CC[CT]
3321 GGGACCT
PstI 791 CTGCAG CTGCA^G
1936 CTGCAG
PvuII 2812 CAGCTG CAG^CTG
3685 CAGCTG
SacI 47 GAGCTC GAGCT^C
3146 GAGCTC
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NsiI PleI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII XmnI