Restriction map for
pFN21ASDA0642
(Linear) (Six-base) MAPSORT of: KIAA0642.seq Check: 5624 from: 1 to: 3960
KIAA0642.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1943 3960
Size: 1943 2017
AatII G_ACGT'C
Cuts at: 0 822 3960
Size: 822 3138
AccI GT'mk_AC
Cuts at: 0 1314 3960
Size: 1314 2646
Acc65I G'GTAC_C
Cuts at: 0 243 3052 3960
Size: 243 2809 908
Fragments arranged by size:
2809 908 243
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 965 1196 1307 1370 2128 2224 2971
Size: 161 804 231 111 63 758 96 747
Cuts at: 2971 3032 3448 3960
Size: 61 416 512
Fragments arranged by size:
804 758 747 512 416 231 161 111
96 63 61
AhdI GACnn_n'nnGTC
Cuts at: 0 1221 3960
Size: 1221 2739
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 478 2483 3960
Size: 478 2005 1477
Fragments arranged by size:
2005 1477 478
AlwNI CAG_nnn'CTG
Cuts at: 0 21 1371 1507 1935 2125 2224 3687 3960
Size: 21 1350 136 428 190 99 1463 273
Fragments arranged by size:
1463 1350 428 273 190 136 99 21
ApaI G_GGCC'C
Cuts at: 0 2616 3960
Size: 2616 1344
ApaLI G'TGCA_C
Cuts at: 0 1577 3960
Size: 1577 2383
ApoI r'AATT_y
Cuts at: 0 196 606 649 1694 2363 3189 3330 3960
Size: 196 410 43 1045 669 826 141 630
Fragments arranged by size:
1045 826 669 630 410 196 141 43
AvaI C'yCGr_G
Cuts at: 0 2615 3960
Size: 2615 1345
AvrII C'CTAG_G
Cuts at: 0 3325 3960
Size: 3325 635
BanI G'GyrC_C
Cuts at: 0 243 2957 3052 3960
Size: 243 2714 95 908
Fragments arranged by size:
2714 908 243 95
BanII G_rGCy'C
Cuts at: 0 51 348 2616 3150 3205 3960
Size: 51 297 2268 534 55 755
Fragments arranged by size:
2268 755 534 297 55 51
BbeI G_GCGC'C
Cuts at: 0 2961 3960
Size: 2961 999
BbsI GAAGACnn'nnnn_
Cuts at: 0 2000 2355 2532 3960
Size: 2000 355 177 1428
Fragments arranged by size:
2000 1428 355 177
BclI T'GATC_A
Cuts at: 0 2076 3960
Size: 2076 1884
BfrBI ATG'CAT
Cuts at: 0 465 1650 3910 3960
Size: 465 1185 2260 50
Fragments arranged by size:
2260 1185 465 50
BglI GCCn_nnn'nGGC
Cuts at: 0 39 3960
Size: 39 3921
Bme1580I G_kGCm'C
Cuts at: 0 1331 1581 2616 3960
Size: 1331 250 1035 1344
Fragments arranged by size:
1344 1331 1035 250
BmrI ACTGGGnnnn_n'
Cuts at: 0 3878 3960
Size: 3878 82
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1083 3960
Size: 1083 2877
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 196 995 1826 2743 3091 3960
Size: 74 122 799 831 917 348 869
Fragments arranged by size:
917 869 831 799 348 122 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3658 3960
Size: 3658 302
BsaI GGTCTCn'nnnn_
Cuts at: 0 594 1528 3960
Size: 594 934 2432
Fragments arranged by size:
2432 934 594
BsaBI GATnn'nnATC
Cuts at: 0 1599 3960
Size: 1599 2361
BsaHI Gr'CG_yC
Cuts at: 0 819 2958 3960
Size: 819 2139 1002
Fragments arranged by size:
2139 1002 819
BsaWI w'CCGG_w
Cuts at: 0 2780 3960
Size: 2780 1180
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 63 93 320 350 1383 1413 3204 3234
Size: 63 30 227 30 1033 30 1791 30
Cuts at: 3234 3960
Size: 726
Fragments arranged by size:
1791 1033 726 227 63 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 71 335 338 341 461 1075 1306 1391
Size: 71 264 3 3 120 614 231 85
Cuts at: 1391 1850 2553 2611 2662 2686 3960
Size: 459 703 58 51 24 1274
Fragments arranged by size:
1274 703 614 459 264 231 120 85
71 58 51 24 3 3
BseYI C'CCAG_C
Cuts at: 0 479 576 1330 2012 2633 3960
Size: 479 97 754 682 621 1327
Fragments arranged by size:
1327 754 682 621 479 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3157 3856 3960
Size: 3157 699 104
Fragments arranged by size:
3157 699 104
BsiHKAI G_wGCw'C
Cuts at: 0 51 1069 1479 1581 1619 3150 3960
Size: 51 1018 410 102 38 1531 810
Fragments arranged by size:
1531 1018 810 410 102 51 38
BsmI GAATG_Cn'
Cuts at: 0 979 3162 3960
Size: 979 2183 798
Fragments arranged by size:
2183 979 798
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3937 3960
Size: 3937 23
Bsp1286I G_dGCh'C
Cuts at: 0 51 348 1069 1331 1479 1581 1619 2616
Size: 51 297 721 262 148 102 38 997
Cuts at: 2616 3150 3205 3960
Size: 534 55 755
Fragments arranged by size:
997 755 721 534 297 262 148 102
55 51 38
BspEI T'CCGG_A
Cuts at: 0 2780 3960
Size: 2780 1180
BspHI T'CATG_A
Cuts at: 0 1811 3960
Size: 1811 2149
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1943 3179 3960
Size: 1943 1236 781
Fragments arranged by size:
1943 1236 781
BsrDI GCAATG_nn'
Cuts at: 0 94 1664 3960
Size: 94 1570 2296
Fragments arranged by size:
2296 1570 94
BsrFI r'CCGG_y
Cuts at: 0 2654 3389 3960
Size: 2654 735 571
Fragments arranged by size:
2654 735 571
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1935 3687 3960
Size: 1935 1752 273
Fragments arranged by size:
1935 1752 273
BstEII G'GTnAC_C
Cuts at: 0 865 3960
Size: 865 3095
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1801 3960
Size: 1801 2159
BstYI r'GATC_y
Cuts at: 0 1680 2193 3074 3879 3960
Size: 1680 513 881 805 81
Fragments arranged by size:
1680 881 805 513 81
Bsu36I CC'TnA_GG
Cuts at: 0 1685 3960
Size: 1685 2275
BtsI GCAGTG_nn'
Cuts at: 0 894 1497 3314 3478 3960
Size: 894 603 1817 164 482
Fragments arranged by size:
1817 894 603 482 164
DrdI GACnn_nn'nnGTC
Cuts at: 0 770 3960
Size: 770 3190
EaeI y'GGCC_r
Cuts at: 0 1450 3960
Size: 1450 2510
EarI CTCTTCn'nnn_
Cuts at: 0 1016 1088 1283 1409 1765 2280 2309 2315
Size: 1016 72 195 126 356 515 29 6
Cuts at: 2315 2429 2483 2528 2538 2543 2588 2663 2704
Size: 114 54 45 10 5 45 75 41
Cuts at: 2704 2942 2990 3071 3080 3960
Size: 238 48 81 9 880
Fragments arranged by size:
1016 880 515 356 238 195 126 114
81 75 72 54 48 45 45 41
29 10 9 6 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2577 3960
Size: 2577 1383
EcoICRI GAG'CTC
Cuts at: 0 49 3148 3960
Size: 49 3099 812
Fragments arranged by size:
3099 812 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 161 196 965 995 1196 1307 1370
Size: 74 87 35 769 30 201 111 63
Cuts at: 1370 1826 2128 2224 2743 2971 3032 3091 3448
Size: 456 302 96 519 228 61 59 357
Cuts at: 3448 3960
Size: 512
Fragments arranged by size:
769 519 512 456 357 302 228 201
111 96 87 74 63 61 59 35
30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 214 3960
Size: 214 3746
EcoO109I rG'GnC_Cy
Cuts at: 0 1702 2221 3322 3587 3960
Size: 1702 519 1101 265 373
Fragments arranged by size:
1702 1101 519 373 265
EcoRI G'AATT_C
Cuts at: 0 3189 3330 3960
Size: 3189 141 630
Fragments arranged by size:
3189 630 141
EcoRV GAT'ATC
Cuts at: 0 1809 3960
Size: 1809 2151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2431 2536 3960
Size: 2431 105 1424
Fragments arranged by size:
2431 1424 105
HaeII r_GCGC'y
Cuts at: 0 2961 3645 3960
Size: 2961 684 315
Fragments arranged by size:
2961 684 315
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1207 1239 1381 1413 2113 2145 2967 2999
Size: 1207 32 142 32 700 32 822 32
Cuts at: 2999 3887 3919 3960
Size: 888 32 41
Fragments arranged by size:
1207 888 822 700 142 41 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1788 2827 3960
Size: 1788 1039 1133
Fragments arranged by size:
1788 1133 1039
KasI G'GCGC_C
Cuts at: 0 2957 3960
Size: 2957 1003
KpnI G_GTAC'C
Cuts at: 0 247 3056 3960
Size: 247 2809 904
Fragments arranged by size:
2809 904 247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 1818 3191 3308 3929 3960
Size: 802 1016 1373 117 621 31
Fragments arranged by size:
1373 1016 802 621 117 31
MslI CAynn'nnrTG
Cuts at: 0 1816 3960
Size: 1816 2144
MspA1I CmG'CkG
Cuts at: 0 7 2454 2733 2814 3687 3960
Size: 7 2447 279 81 873 273
Fragments arranged by size:
2447 873 279 273 81 7
NarI GG'CG_CC
Cuts at: 0 2958 3960
Size: 2958 1002
NdeI CA'TA_TG
Cuts at: 0 1865 3960
Size: 1865 2095
NsiI A_TGCA'T
Cuts at: 0 467 1652 3912 3960
Size: 467 1185 2260 48
Fragments arranged by size:
2260 1185 467 48
NspI r_CATG'y
Cuts at: 0 558 2876 3960
Size: 558 2318 1084
Fragments arranged by size:
2318 1084 558
PflMI CCAn_nnn'nTGG
Cuts at: 0 2125 3960
Size: 2125 1835
PfoI T'CCnGG_A
Cuts at: 0 212 3960
Size: 212 3748
PpuMI rG'GwC_Cy
Cuts at: 0 1702 3322 3960
Size: 1702 1620 638
Fragments arranged by size:
1702 1620 638
PspOMI G'GGCC_C
Cuts at: 0 2612 3960
Size: 2612 1348
PstI C_TGCA'G
Cuts at: 0 795 1940 3960
Size: 795 1145 2020
Fragments arranged by size:
2020 1145 795
PvuII CAG'CTG
Cuts at: 0 2814 3687 3960
Size: 2814 873 273
Fragments arranged by size:
2814 873 273
SacI G_AGCT'C
Cuts at: 0 51 3150 3960
Size: 51 3099 810
Fragments arranged by size:
3099 810 51
SapI GCTCTTCn'nnn_
Cuts at: 0 1016 2315 2942 2990 3960
Size: 1016 1299 627 48 970
Fragments arranged by size:
1299 1016 970 627 48
SexAI A'CCwGG_T
Cuts at: 0 1200 3960
Size: 1200 2760
SfcI C'TryA_G
Cuts at: 0 791 1936 2165 3374 3960
Size: 791 1145 229 1209 586
Fragments arranged by size:
1209 1145 791 586 229
SfoI GGC'GCC
Cuts at: 0 2959 3960
Size: 2959 1001
SmaI CCC'GGG
Cuts at: 0 2617 3960
Size: 2617 1343
SmlI C'TyrA_G
Cuts at: 0 3637 3960
Size: 3637 323
StyI C'CwwG_G
Cuts at: 0 594 3325 3614 3960
Size: 594 2731 289 346
Fragments arranged by size:
2731 594 346 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3922 3960
Size: 3922 38
TatI w'GTAC_w
Cuts at: 0 557 999 3772 3960
Size: 557 442 2773 188
Fragments arranged by size:
2773 557 442 188
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 320 323 352 355 3739 3771 3960
Size: 320 3 29 3 3384 32 189
Fragments arranged by size:
3384 320 189 32 29 3 3
Tth111I GACn'n_nGTC
Cuts at: 0 3244 3960
Size: 3244 716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2895 3960
Size: 2895 1065
XmaI C'CCGG_G
Cuts at: 0 2615 3960
Size: 2615 1345
XmnI GAAnn'nnTTC
Cuts at: 0 224 273 363 1733 2237 2366 3960
Size: 224 49 90 1370 504 129 1594
Fragments arranged by size:
1594 1370 504 224 129 90 49
ZraI GAC'GTC
Cuts at: 0 820 3960
Size: 820 3140
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AhdI AlfI AlwNI
ApaI ApaLI ApoI AvaI AvrII BanI BanII BbeI
BbsI BclI BfrBI BglI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtsI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HindIII KasI KpnI
MmeI MslI MspA1I NarI NdeI NsiI NspI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SapI SexAI
SfcI SfoI SmaI SmlI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AflIII AgeI AleI AloI AscI
AseI AsiSI BaeI BamHI BbvCI BcgI BciVI BglII
BlpI BmgBI BmtI Bpu10I BsaAI BsiEI BsiWI BsrBI
BsrGI BssHII BssSI BstBI BstZ17I BtgI BtgZI ClaI
CspCI DraI DraIII EagI FseI FspI FspAI HincII
HpaI MfeI MluI MscI NaeI NcoI NgoMIV NheI
NotI NruI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XhoI