(Circular) (Six-base) MAPSORT of: pFN21ASDA0637.seq Check: 5966 from: 1 to: 8242 pFN21ASDA0637 8242 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4333 4333 Size: 8242 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4686 6052 278 Size: 53 83 186 936 3150 1366 2468 Fragments arranged by size: 3150 2468 1366 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4050 5585 1937 Size: 2113 1535 4594 Fragments arranged by size: 4594 2113 1535 Acc65I G'GTAC_C Cuts at: 3809 5563 3809 Size: 1754 6488 AclI AA'CG_TT Cuts at: 1563 4056 6369 6742 1563 Size: 2493 2313 373 3063 Fragments arranged by size: 3063 2493 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2739 3413 4409 4489 4997 5286 5360 5397 Size: 1107 674 996 80 508 289 74 37 Cuts at: 5397 6305 7689 7957 1632 Size: 908 1384 268 1917 Fragments arranged by size: 1917 1384 1107 996 908 674 508 289 268 80 74 37 AflII C'TTAA_G Cuts at: 820 1017 4181 820 Size: 197 3164 4881 Fragments arranged by size: 4881 3164 197 AflIII A'CryG_T Cuts at: 1203 2736 3012 4335 7141 8118 1203 Size: 1533 276 1323 2806 977 1327 Fragments arranged by size: 2806 1533 1327 1323 977 276 AgeI A'CCGG_T Cuts at: 4605 7073 4605 Size: 2468 5774 AhdI GACnn_n'nnGTC Cuts at: 3214 4687 6971 3214 Size: 1473 2284 4485 Fragments arranged by size: 4485 2284 1473 AleI CACnn'nnGTG Cuts at: 1166 3905 5166 5313 1166 Size: 2739 1261 147 4095 Fragments arranged by size: 4095 2739 1261 147 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8242 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2345 2377 2345 Size: 32 8210 AlwNI CAG_nnn'CTG Cuts at: 4567 5985 7557 4567 Size: 1418 1572 5252 Fragments arranged by size: 5252 1572 1418 ApaLI G'TGCA_C Cuts at: 4601 6299 7455 4601 Size: 1698 1156 5388 Fragments arranged by size: 5388 1698 1156 ApoI r'AATT_y Cuts at: 1493 2115 5131 5551 5777 5929 8038 1493 Size: 622 3016 420 226 152 2109 1697 Fragments arranged by size: 3016 2109 1697 622 420 226 152 AseI AT'TA_AT Cuts at: 160 1032 6796 160 Size: 872 5764 1606 Fragments arranged by size: 5764 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8242 AvaI C'yCGr_G Cuts at: 1870 1943 2233 2406 3903 4489 4718 5567 1870 Size: 73 290 173 1497 586 229 849 4545 Fragments arranged by size: 4545 1497 849 586 290 229 173 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2464 2497 3793 3826 2464 Size: 33 1296 33 6880 Fragments arranged by size: 6880 1296 33 33 BamHI G'GATC_C Cuts at: 5047 5572 5939 5047 Size: 525 367 7350 Fragments arranged by size: 7350 525 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3353 3500 3531 3809 3907 Size: 325 217 627 1566 147 31 278 98 Cuts at: 3907 5563 7018 618 Size: 1656 1455 1842 Fragments arranged by size: 1842 1656 1566 1455 627 325 278 217 147 98 31 BanII G_rGCy'C Cuts at: 1391 2363 3129 5561 1391 Size: 972 766 2432 4072 Fragments arranged by size: 4072 2432 972 766 BbeI G_GCGC'C Cuts at: 3535 3535 Size: 8242 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3255 3812 4472 5023 928 Size: 971 1356 557 660 551 4147 Fragments arranged by size: 4147 1356 971 660 557 551 BbvCI CC'TCA_GC Cuts at: 2618 4662 4883 2618 Size: 2044 221 5977 Fragments arranged by size: 5977 2044 221 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2801 2835 6433 6467 1903 Size: 34 864 34 3598 34 3678 Fragments arranged by size: 3678 3598 864 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6134 7344 6134 Size: 1210 7032 BclI T'GATC_A Cuts at: 1548 4785 1548 Size: 3237 5005 BfrBI ATG'CAT Cuts at: 2430 2430 Size: 8242 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2321 3561 3605 Size: 107 122 71 631 605 649 1240 44 Cuts at: 3605 4294 6853 136 Size: 689 2559 1525 Fragments arranged by size: 2559 1525 1240 689 649 631 605 122 107 71 44 BglII A'GATC_T Cuts at: 4587 8237 4587 Size: 3650 4592 BlpI GC'TnA_GC Cuts at: 3128 4571 5659 3128 Size: 1443 1088 5711 Fragments arranged by size: 5711 1443 1088 Bme1580I G_kGCm'C Cuts at: 1790 2993 3025 3059 3503 3920 4605 6303 7459 Size: 1203 32 34 444 417 685 1698 1156 Cuts at: 7459 1790 Size: 2573 Fragments arranged by size: 2573 1698 1203 1156 685 444 417 34 32 BmgBI CAC'GTC Cuts at: 1318 2311 3253 4215 4336 5149 1318 Size: 993 942 962 121 813 4411 Fragments arranged by size: 4411 993 962 942 813 121 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2281 3974 4279 Size: 416 53 83 399 24 864 1693 305 Cuts at: 4279 5973 6931 442 Size: 1694 958 1753 Fragments arranged by size: 1753 1694 1693 958 864 416 399 305 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8242 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2219 3148 2219 Size: 929 7313 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2347 2361 3252 4016 4108 4406 5309 6902 Size: 1108 14 891 764 92 298 903 1593 Cuts at: 6902 1239 Size: 2579 Fragments arranged by size: 2579 1593 1108 903 891 764 298 92 14 Bpu10I CC'TnA_GC Cuts at: 2618 4662 4883 2618 Size: 2044 221 5977 Fragments arranged by size: 5977 2044 221 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2497 3425 6367 7232 7530 2497 Size: 928 2942 865 298 3209 Fragments arranged by size: 3209 2942 928 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3874 6905 882 Size: 617 2375 3031 2219 Fragments arranged by size: 3031 2375 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2503 5539 6033 493 Size: 711 1299 3036 494 2702 Fragments arranged by size: 3036 2702 1299 711 494 BsaBI GATnn'nnATC Cuts at: 4952 8236 4952 Size: 3284 4958 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2660 3296 3532 4683 Size: 53 83 186 936 1127 636 236 1151 Cuts at: 4683 5348 6049 6431 7066 275 Size: 665 701 382 635 1451 Fragments arranged by size: 1451 1151 1127 936 701 665 636 635 382 236 186 83 53 BsaWI w'CCGG_w Cuts at: 3148 3895 4605 6675 7073 7347 7494 7905 3148 Size: 747 710 2070 398 274 147 411 3485 Fragments arranged by size: 3485 2070 747 710 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2345 2375 2687 2717 4014 Size: 30 165 30 942 30 312 30 1297 Cuts at: 4014 4044 4379 4409 5048 5078 5198 5228 1178 Size: 30 335 30 639 30 120 30 4192 Fragments arranged by size: 4192 1297 942 639 335 312 165 120 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2708 2719 3172 3175 3880 4955 5093 5096 Size: 1522 11 453 3 705 1075 138 3 Cuts at: 5096 5219 5306 5309 1186 Size: 123 87 3 4119 Fragments arranged by size: 4119 1522 1075 705 453 138 123 87 11 3 3 3 BseYI C'CCAG_C Cuts at: 7445 7941 7445 Size: 496 7746 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4517 4580 1163 Size: 573 2781 63 4825 Fragments arranged by size: 4825 2781 573 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2523 2683 4298 4610 5692 6453 Size: 275 186 522 160 1615 312 1082 761 Cuts at: 6453 7481 1540 Size: 1028 2301 Fragments arranged by size: 2301 1615 1082 1028 761 522 312 275 186 160 BsiHKAI G_wGCw'C Cuts at: 2363 2529 2864 3129 4488 4605 4703 4868 6303 Size: 166 335 265 1359 117 98 165 1435 Cuts at: 6303 6388 7459 2363 Size: 85 1071 3146 Fragments arranged by size: 3146 1435 1359 1071 335 265 166 165 117 98 85 BsmI GAATG_Cn' Cuts at: 2940 4371 5753 5846 2940 Size: 1431 1382 93 5336 Fragments arranged by size: 5336 1431 1382 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2790 3150 3869 4937 2790 Size: 360 719 1068 6095 Fragments arranged by size: 6095 1068 719 360 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2363 2529 2864 2993 3025 3059 3129 Size: 399 573 166 335 129 32 34 70 Cuts at: 3129 3503 3920 4488 4605 4703 4868 5561 6303 Size: 374 417 568 117 98 165 693 742 Cuts at: 6303 6388 7459 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 693 573 568 417 399 374 335 166 165 129 117 98 85 70 34 32 BspEI T'CCGG_A Cuts at: 3895 7905 3895 Size: 4010 4232 BspHI T'CATG_A Cuts at: 2493 3381 5127 5469 6131 7861 2493 Size: 888 1746 342 662 1730 2874 Fragments arranged by size: 2874 1746 1730 888 662 342 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2827 4333 4575 5597 844 Size: 1983 1506 242 1022 3489 Fragments arranged by size: 3489 1983 1506 1022 242 BsrBI CCG'CTC Cuts at: 1394 1823 2198 4597 4723 5323 6129 1394 Size: 429 375 2399 126 600 806 3507 Fragments arranged by size: 3507 2399 806 600 429 375 126 BsrDI GCAATG_nn' Cuts at: 65 1248 6737 6911 65 Size: 1183 5489 174 1396 Fragments arranged by size: 5489 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3157 3528 4418 4605 6886 7073 1700 Size: 110 1347 371 890 187 2281 187 2869 Fragments arranged by size: 2869 2281 1347 890 371 187 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8242 BssHII G'CGCG_C Cuts at: 1925 2274 2509 1925 Size: 349 235 7658 Fragments arranged by size: 7658 349 235 BssSI C'ACGA_G Cuts at: 2992 6302 7314 2992 Size: 3310 1012 3920 Fragments arranged by size: 3920 3310 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2946 2997 3507 4567 794 Size: 2152 51 510 1060 4469 Fragments arranged by size: 4469 2152 1060 510 51 BstBI TT'CG_AA Cuts at: 2007 7046 2007 Size: 5039 3203 BstEII G'GTnAC_C Cuts at: 1184 2612 4408 1184 Size: 1428 1796 5018 Fragments arranged by size: 5018 1796 1428 BstXI CCAn_nnnn'nTGG Cuts at: 5044 5044 Size: 8242 BstYI r'GATC_y Cuts at: 1972 2667 4587 5047 5572 5939 6324 6341 7793 Size: 695 1920 460 525 367 385 17 1452 Cuts at: 7793 7879 7891 8237 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1920 1452 695 525 460 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2316 3429 3911 4899 4986 5021 Size: 552 957 294 1113 482 988 87 35 Cuts at: 5021 5271 513 Size: 250 3484 Fragments arranged by size: 3484 1113 988 957 552 482 294 250 87 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7503 Fragments arranged by size: 7503 683 56 BtsI GCAGTG_nn' Cuts at: 2944 4315 4505 5758 6552 6572 2944 Size: 1371 190 1253 794 20 4614 Fragments arranged by size: 4614 1371 1253 794 190 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8242 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8207 DraI TTT'AAA Cuts at: 2089 2149 5546 2089 Size: 60 3397 4785 Fragments arranged by size: 4785 3397 60 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7249 809 Size: 507 5933 1802 Fragments arranged by size: 5933 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2313 3769 4295 Size: 54 1425 50 275 18 483 1456 526 Cuts at: 4295 4632 5226 5354 5373 5455 5689 6578 8207 Size: 337 594 128 19 82 234 889 1629 Cuts at: 8207 8 Size: 43 Fragments arranged by size: 1629 1456 1425 889 594 526 483 337 275 234 128 82 54 50 43 19 18 EagI C'GGCC_G Cuts at: 1537 1812 4295 5689 1537 Size: 275 2483 1394 4090 Fragments arranged by size: 4090 2483 1394 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2091 2834 3631 4940 6172 1353 Size: 738 743 797 1309 1232 3423 Fragments arranged by size: 3423 1309 1232 797 743 738 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5102 6829 7203 7349 387 Size: 1064 138 3513 1727 374 146 1280 Fragments arranged by size: 3513 1727 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2361 3127 2361 Size: 766 7476 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2347 2361 2739 3252 3413 4016 4108 Size: 393 715 14 378 513 161 603 92 Cuts at: 4108 4406 4409 4489 4997 5286 5309 5360 5397 Size: 298 3 80 508 289 23 51 37 Cuts at: 5397 6305 6902 7689 7957 1239 Size: 908 597 787 268 1524 Fragments arranged by size: 1524 908 787 715 603 597 513 508 393 378 298 289 268 161 92 80 51 37 23 14 3 EcoNI CCTnn'n_nnAGG Cuts at: 4676 4886 5161 5342 4676 Size: 210 275 181 7576 Fragments arranged by size: 7576 275 210 181 EcoO109I rG'GnC_Cy Cuts at: 2983 2983 Size: 8242 EcoRI G'AATT_C Cuts at: 2115 5131 5551 5929 2115 Size: 3016 420 378 4428 Fragments arranged by size: 4428 3016 420 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3642 5528 3642 Size: 1886 6356 FspI TGC'GCA Cuts at: 6748 6748 Size: 8242 HaeII r_GCGC'y Cuts at: 1678 3535 3936 4328 7389 1678 Size: 1857 401 392 3061 2531 Fragments arranged by size: 3061 2531 1857 401 392 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2687 2719 3200 3232 3505 Size: 32 135 32 1115 32 481 32 273 Cuts at: 3505 3537 4673 4705 4770 4802 5162 5194 6883 Size: 32 1136 32 65 32 360 32 1689 Cuts at: 6883 6915 6957 6989 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1689 1136 1115 481 360 273 135 65 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5586 5832 669 Size: 988 281 3648 246 3079 Fragments arranged by size: 3648 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8242 HpaI GTT'AAC Cuts at: 5832 5832 Size: 8242 KasI G'GCGC_C Cuts at: 3531 3531 Size: 8242 KpnI G_GTAC'C Cuts at: 3813 5567 3813 Size: 1754 6488 MfeI C'AATT_G Cuts at: 5841 5841 Size: 8242 MluI A'CGCG_T Cuts at: 8118 8118 Size: 8242 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3378 4311 7356 7540 1305 Size: 2073 933 3045 184 2007 Fragments arranged by size: 3045 2073 2007 933 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2315 5228 5457 10 Size: 54 1425 343 483 2913 229 2795 Fragments arranged by size: 2913 2795 1425 483 343 229 54 MslI CAynn'nnrTG Cuts at: 518 1166 2811 3905 4871 4898 5166 5313 6200 Size: 648 1645 1094 966 27 268 147 887 Cuts at: 6200 6559 6718 518 Size: 359 159 2042 Fragments arranged by size: 2042 1645 1094 966 887 648 359 268 159 147 27 MspA1I CmG'CkG Cuts at: 1606 2184 2223 2888 3507 3816 3838 3913 4384 Size: 578 39 665 619 309 22 75 471 Cuts at: 4384 4552 4791 5023 5313 5658 6335 7483 7728 Size: 168 239 232 290 345 677 1148 245 Cuts at: 7728 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 665 619 578 471 345 309 290 245 239 232 168 75 39 22 NarI GG'CG_CC Cuts at: 3532 3532 Size: 8242 NcoI C'CATG_G Cuts at: 513 1065 2022 2316 3429 4899 4986 5271 513 Size: 552 957 294 1113 1470 87 285 3484 Fragments arranged by size: 3484 1470 1113 957 552 294 285 87 NdeI CA'TA_TG Cuts at: 387 387 Size: 8242 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8242 NotI GC'GGCC_GC Cuts at: 5689 5689 Size: 8242 NsiI A_TGCA'T Cuts at: 2432 2432 Size: 8242 NspI r_CATG'y Cuts at: 1132 1229 2434 2740 3016 4717 5600 7145 1132 Size: 97 1205 306 276 1701 883 1545 2229 Fragments arranged by size: 2229 1701 1545 1205 883 306 276 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8242 PciI A'CATG_T Cuts at: 2736 3012 7141 2736 Size: 276 4129 3837 Fragments arranged by size: 4129 3837 276 PflMI CCAn_nnn'nTGG Cuts at: 1108 2656 4555 1108 Size: 1548 1899 4795 Fragments arranged by size: 4795 1899 1548 PfoI T'CCnGG_A Cuts at: 2363 2873 4124 4677 2363 Size: 510 1251 553 5928 Fragments arranged by size: 5928 1251 553 510 PmeI GTTT'AAAC Cuts at: 5546 5546 Size: 8242 PmlI CAC'GTG Cuts at: 2503 2503 Size: 8242 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2345 2377 5109 5141 6311 6343 7824 7856 2345 Size: 32 2732 32 1170 32 1481 32 2731 Fragments arranged by size: 2732 2731 1481 1170 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2983 2983 Size: 8242 PshAI GACnn'nnGTC Cuts at: 1613 3394 1613 Size: 1781 6461 PsiI TTA'TAA Cuts at: 5812 5812 Size: 8242 PspXI vC'TCGA_Gb Cuts at: 4489 4718 4489 Size: 229 8013 PstI C_TGCA'G Cuts at: 830 2254 2824 3616 4644 5594 830 Size: 1424 570 792 1028 950 3478 Fragments arranged by size: 3478 1424 1028 950 792 570 PvuI CG_AT'CG Cuts at: 2001 2683 2001 Size: 682 7560 PvuII CAG'CTG Cuts at: 2223 3507 3816 4384 4791 5313 2223 Size: 1284 309 568 407 522 5152 Fragments arranged by size: 5152 1284 568 522 407 309 SacI G_AGCT'C Cuts at: 2363 3129 2363 Size: 766 7476 SacII CC_GC'GG Cuts at: 3914 5024 3914 Size: 1110 7132 SalI G'TCGA_C Cuts at: 1936 5584 1936 Size: 3648 4594 SapI GCTCTTCn'nnn_ Cuts at: 2834 4940 2834 Size: 2106 6136 SbfI CC_TGCA'GG Cuts at: 5594 5594 Size: 8242 ScaI AGT'ACT Cuts at: 2243 4097 6490 8202 2243 Size: 1854 2393 1712 2283 Fragments arranged by size: 2393 2283 1854 1712 SfcI C'TryA_G Cuts at: 826 1045 2250 2706 2820 3612 4357 4640 5590 Size: 219 1205 456 114 792 745 283 950 Cuts at: 5590 6725 7406 7597 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1205 1135 950 792 745 681 456 283 219 191 114 SfiI GGCCn_nnn'nGGCC Cuts at: 2321 2321 Size: 8242 SfoI GGC'GCC Cuts at: 3533 3533 Size: 8242 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8242 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8242 SmaI CCC'GGG Cuts at: 1872 2235 3905 5569 1872 Size: 363 1670 1664 4545 Fragments arranged by size: 4545 1670 1664 363 SmlI C'TyrA_G Cuts at: 820 1017 1943 2476 3440 4181 4489 4718 6346 Size: 197 926 533 964 741 308 229 1628 Cuts at: 6346 7247 7509 820 Size: 901 262 1553 Fragments arranged by size: 1628 1553 964 926 901 741 533 308 262 229 197 SnaBI TAC'GTA Cuts at: 493 5539 6033 493 Size: 5046 494 2702 Fragments arranged by size: 5046 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8242 SphI G_CATG'C Cuts at: 1132 5600 1132 Size: 4468 3774 SspI AAT'ATT Cuts at: 5 52 6166 5 Size: 47 6114 2081 Fragments arranged by size: 6114 2081 47 StuI AGG'CCT Cuts at: 1517 3290 5063 1517 Size: 1773 1773 4696 Fragments arranged by size: 4696 1773 1773 StyI C'CwwG_G Cuts at: 513 1065 2022 2316 3429 4614 4899 4986 5229 Size: 552 957 294 1113 1185 285 87 243 Cuts at: 5229 5271 5452 5681 513 Size: 42 181 229 3074 Fragments arranged by size: 3074 1185 1113 957 552 294 285 243 229 181 87 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2650 3560 3993 4396 5449 6269 6286 Size: 1255 1070 910 433 403 1053 820 17 Cuts at: 6286 6439 6624 7069 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1070 1053 910 820 445 433 403 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2241 2289 2433 Size: 274 80 33 51 1443 264 48 144 Cuts at: 2433 3075 4095 5094 6488 8200 96 Size: 642 1020 999 1394 1712 138 Fragments arranged by size: 1712 1443 1394 1020 999 642 274 264 144 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3475 3507 4014 4046 4259 4291 4379 4411 5198 Size: 32 507 32 213 32 88 32 787 Cuts at: 5198 5230 8202 8234 3475 Size: 32 2972 32 3483 Fragments arranged by size: 3483 2972 787 507 213 88 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8242 XbaI T'CTAG_A Cuts at: 5578 5578 Size: 8242 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4214 5470 4214 Size: 1256 6986 XhoI C'TCGA_G Cuts at: 1943 4489 4718 1943 Size: 2546 229 5467 Fragments arranged by size: 5467 2546 229 XmaI C'CCGG_G Cuts at: 1870 2233 3903 5567 1870 Size: 363 1670 1664 4545 Fragments arranged by size: 4545 1670 1664 363 XmnI GAAnn'nnTTC Cuts at: 2447 2900 2957 3563 6371 2447 Size: 453 57 606 2808 4318 Fragments arranged by size: 4318 2808 606 453 57 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4684 6050 276 Size: 53 83 186 936 3150 1366 2468 Fragments arranged by size: 3150 2468 1366 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI PacI PspOMI PsrI RsrII SanDI SexAI SrfI SwaI