(Circular) (Six-base) MAPSORT of: pFN21ASDA0438.seq Check: 7021 from: 1 to: 6853 pFN21ASDA0438 6853 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4069 4069 Size: 6853 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4663 278 Size: 53 83 186 936 3127 2468 Fragments arranged by size: 3127 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4196 1937 Size: 2259 4594 Acc65I G'GTAC_C Cuts at: 4174 4174 Size: 6853 AclI AA'CG_TT Cuts at: 1563 4980 5353 1563 Size: 3417 373 3063 Fragments arranged by size: 3417 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2892 3467 3477 3732 4916 6300 6568 1632 Size: 1260 575 10 255 1184 1384 268 1917 Fragments arranged by size: 1917 1384 1260 1184 575 268 255 10 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6656 AflIII A'CryG_T Cuts at: 1203 5752 6729 1203 Size: 4549 977 1327 Fragments arranged by size: 4549 1327 977 AgeI A'CCGG_T Cuts at: 5684 5684 Size: 6853 AhdI GACnn_n'nnGTC Cuts at: 5582 5582 Size: 6853 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6853 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6853 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3722 3754 3722 Size: 32 6821 AlwNI CAG_nnn'CTG Cuts at: 4596 6168 4596 Size: 1572 5281 ApaLI G'TGCA_C Cuts at: 4910 6066 4910 Size: 1156 5697 ApoI r'AATT_y Cuts at: 1493 2245 2325 2787 3519 3587 4162 4388 4540 Size: 752 80 462 732 68 575 226 152 Cuts at: 4540 6649 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 752 732 575 462 226 152 80 68 AseI AT'TA_AT Cuts at: 160 1032 5407 160 Size: 872 4375 1606 Fragments arranged by size: 4375 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6853 AvaI C'yCGr_G Cuts at: 1870 1943 4178 1870 Size: 73 2235 4545 Fragments arranged by size: 4545 2235 73 BamHI G'GATC_C Cuts at: 3151 3730 4183 4550 3151 Size: 579 453 367 5454 Fragments arranged by size: 5454 579 453 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4174 5629 618 Size: 325 217 627 2387 1455 1842 Fragments arranged by size: 2387 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4172 1391 Size: 2781 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2143 2790 3660 3860 928 Size: 971 244 647 870 200 3921 Fragments arranged by size: 3921 971 870 647 244 200 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5044 5078 1903 Size: 34 3107 34 3678 Fragments arranged by size: 3678 3107 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2404 4745 5955 2404 Size: 2341 1210 3302 Fragments arranged by size: 3302 2341 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6853 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5464 136 Size: 107 122 71 631 605 3792 1525 Fragments arranged by size: 3792 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6848 6848 Size: 6853 BlpI GC'TnA_GC Cuts at: 3769 4270 3769 Size: 501 6352 Bme1580I G_kGCm'C Cuts at: 1790 4914 6070 1790 Size: 3124 1156 2573 Fragments arranged by size: 3124 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6853 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2286 4584 5542 Size: 416 53 83 399 24 869 2298 958 Cuts at: 5542 442 Size: 1753 Fragments arranged by size: 2298 1753 958 869 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6853 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2817 3655 2817 Size: 838 6015 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3625 3650 5513 1239 Size: 2386 25 1863 2579 Fragments arranged by size: 2579 2386 1863 25 Bpu10I CC'TnA_GC Cuts at: 2188 3429 2188 Size: 1241 5612 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2787 3148 3364 4978 5843 6141 2787 Size: 361 216 1614 865 298 3499 Fragments arranged by size: 3499 1614 865 361 298 216 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3869 5516 882 Size: 617 2370 1647 2219 Fragments arranged by size: 2370 2219 1647 617 BsaAI yAC'GTr Cuts at: 493 1204 4150 4644 493 Size: 711 2946 494 2702 Fragments arranged by size: 2946 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6847 6847 Size: 6853 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4660 5042 5677 275 Size: 53 83 186 936 3127 382 635 1451 Fragments arranged by size: 3127 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5286 5684 5958 6105 6516 5286 Size: 398 274 147 411 5623 Fragments arranged by size: 5623 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6628 Fragments arranged by size: 6628 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6853 BseYI C'CCAG_C Cuts at: 3393 6056 6552 3393 Size: 2663 496 3694 Fragments arranged by size: 3694 2663 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6280 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4303 5064 6092 1540 Size: 275 186 2302 761 1028 2301 Fragments arranged by size: 2302 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4914 4999 6070 4914 Size: 85 1071 5697 Fragments arranged by size: 5697 1071 85 BsmI GAATG_Cn' Cuts at: 3317 4364 4457 3317 Size: 1047 93 5713 Fragments arranged by size: 5713 1047 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4172 4914 4999 6070 1391 Size: 399 2382 742 85 1071 2174 Fragments arranged by size: 2382 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6516 6516 Size: 6853 BspHI T'CATG_A Cuts at: 4742 6472 4742 Size: 1730 5123 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3586 4069 4208 844 Size: 2742 483 139 3489 Fragments arranged by size: 3489 2742 483 139 BsrBI CCG'CTC Cuts at: 1394 1823 4740 1394 Size: 429 2917 3507 Fragments arranged by size: 3507 2917 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2061 5348 5522 65 Size: 1183 813 3287 174 1396 Fragments arranged by size: 3287 1396 1183 813 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5497 5684 1700 Size: 110 3687 187 2869 Fragments arranged by size: 3687 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6853 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6853 BssSI C'ACGA_G Cuts at: 4913 5925 4913 Size: 1012 5841 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6853 BstBI TT'CG_AA Cuts at: 2007 5657 2007 Size: 3650 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6853 BstXI CCAn_nnnn'nTGG Cuts at: 2069 2266 2069 Size: 197 6656 BstYI r'GATC_y Cuts at: 1972 3151 3730 4183 4550 4935 4952 6404 6490 Size: 1179 579 453 367 385 17 1452 86 Cuts at: 6490 6502 6848 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1179 579 453 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2251 2416 2251 Size: 165 6688 BtgI C'CryG_G Cuts at: 513 1065 2938 513 Size: 552 1873 4428 Fragments arranged by size: 4428 1873 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6114 Fragments arranged by size: 6114 683 56 BtsI GCAGTG_nn' Cuts at: 3322 3454 3565 3822 3897 3943 4369 5163 5183 Size: 132 111 257 75 46 426 794 20 Cuts at: 5183 3322 Size: 4992 Fragments arranged by size: 4992 794 426 257 132 111 75 46 20 ClaI AT'CG_AT Cuts at: 1554 3349 1554 Size: 1795 5058 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2982 3017 529 Size: 35 2418 35 4365 Fragments arranged by size: 4365 2418 35 35 DraI TTT'AAA Cuts at: 2158 4157 2158 Size: 1999 4854 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5860 809 Size: 507 4544 1802 Fragments arranged by size: 4544 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3831 4300 5189 Size: 54 1425 50 275 18 2001 469 889 Cuts at: 5189 6818 8 Size: 1629 43 Fragments arranged by size: 2001 1629 1425 889 469 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4300 1537 Size: 275 2488 4090 Fragments arranged by size: 4090 2488 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2709 3486 3504 4783 1353 Size: 1356 777 18 1279 3423 Fragments arranged by size: 3423 1356 1279 777 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5440 5814 5960 387 Size: 1064 138 3851 374 146 1280 Fragments arranged by size: 3851 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2892 3467 3477 3625 3650 3732 4916 Size: 393 1260 575 10 148 25 82 1184 Cuts at: 4916 5513 6300 6568 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1260 1184 787 597 575 393 268 148 82 25 10 EcoRI G'AATT_C Cuts at: 4162 4540 4162 Size: 378 6475 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2160 2160 Size: 6853 FspI TGC'GCA Cuts at: 5359 5359 Size: 6853 HaeII r_GCGC'y Cuts at: 1678 6000 1678 Size: 4322 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 3362 3394 5494 Size: 32 135 32 479 32 1279 32 2100 Cuts at: 5494 5526 5568 5600 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2100 1279 479 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4197 4443 669 Size: 988 281 2259 246 3079 Fragments arranged by size: 3079 2259 988 281 246 HindIII A'AGCT_T Cuts at: 748 2185 748 Size: 1437 5416 HpaI GTT'AAC Cuts at: 4443 4443 Size: 6853 KpnI G_GTAC'C Cuts at: 4178 4178 Size: 6853 MfeI C'AATT_G Cuts at: 4452 4452 Size: 6853 MluI A'CGCG_T Cuts at: 6729 6729 Size: 6853 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2199 2613 3591 5967 6151 1305 Size: 894 414 978 2376 184 2007 Fragments arranged by size: 2376 2007 978 894 414 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3833 10 Size: 54 1425 343 2001 3030 Fragments arranged by size: 3030 2001 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2145 2394 4811 5170 5329 518 Size: 648 979 249 2417 359 159 2042 Fragments arranged by size: 2417 2042 979 648 359 249 159 MspA1I CmG'CkG Cuts at: 1606 3227 4269 4946 6094 6339 1606 Size: 1621 1042 677 1148 245 2120 Fragments arranged by size: 2120 1621 1148 1042 677 245 NcoI C'CATG_G Cuts at: 513 1065 2938 513 Size: 552 1873 4428 Fragments arranged by size: 4428 1873 552 NdeI CA'TA_TG Cuts at: 387 3724 387 Size: 3337 3516 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6853 NotI GC'GGCC_GC Cuts at: 4300 4300 Size: 6853 NspI r_CATG'y Cuts at: 1132 1229 2144 4035 4211 5756 1132 Size: 97 915 1891 176 1545 2229 Fragments arranged by size: 2229 1891 1545 915 176 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6853 PciI A'CATG_T Cuts at: 5752 5752 Size: 6853 PflMI CCAn_nnn'nTGG Cuts at: 1108 3781 1108 Size: 2673 4180 PfoI T'CCnGG_A Cuts at: 2464 2464 Size: 6853 PmeI GTTT'AAAC Cuts at: 4157 4157 Size: 6853 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4922 4954 6435 6467 4922 Size: 32 1481 32 5308 Fragments arranged by size: 5308 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6853 PsiI TTA'TAA Cuts at: 4423 4423 Size: 6853 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3417 3449 3417 Size: 32 6821 PstI C_TGCA'G Cuts at: 830 2578 4084 4205 830 Size: 1748 1506 121 3478 Fragments arranged by size: 3478 1748 1506 121 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6853 SalI G'TCGA_C Cuts at: 1936 4195 1936 Size: 2259 4594 SbfI CC_TGCA'GG Cuts at: 4205 4205 Size: 6853 ScaI AGT'ACT Cuts at: 5101 6813 5101 Size: 1712 5141 SfcI C'TryA_G Cuts at: 826 1045 2574 4080 4201 5336 6017 6208 826 Size: 219 1529 1506 121 1135 681 191 1471 Fragments arranged by size: 1529 1506 1471 1135 681 219 191 121 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6853 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6853 SmaI CCC'GGG Cuts at: 1872 4180 1872 Size: 2308 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2802 3163 3379 4957 5858 6120 Size: 197 926 859 361 216 1578 901 262 Cuts at: 6120 820 Size: 1553 Fragments arranged by size: 1578 1553 926 901 859 361 262 216 197 SnaBI TAC'GTA Cuts at: 493 4150 4644 493 Size: 3657 494 2702 Fragments arranged by size: 3657 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6853 SphI G_CATG'C Cuts at: 1132 4211 1132 Size: 3079 3774 SspI AAT'ATT Cuts at: 5 52 4777 5 Size: 47 4725 2081 Fragments arranged by size: 4725 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6853 StyI C'CwwG_G Cuts at: 513 1065 2938 3504 3524 4292 513 Size: 552 1873 566 20 768 3074 Fragments arranged by size: 3074 1873 768 566 552 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4880 4897 5050 5235 5680 325 Size: 1255 3300 17 153 185 445 1498 Fragments arranged by size: 3300 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2031 2386 2426 Size: 274 80 33 51 1443 54 355 40 Cuts at: 2426 3534 5099 6811 96 Size: 1108 1565 1712 138 Fragments arranged by size: 1712 1565 1443 1108 355 274 138 80 54 51 40 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3250 3282 6813 6845 3250 Size: 32 3531 32 3258 Fragments arranged by size: 3531 3258 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6853 XbaI T'CTAG_A Cuts at: 4189 4189 Size: 6853 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6853 XmaI C'CCGG_G Cuts at: 1870 4178 1870 Size: 2308 4545 XmnI GAAnn'nnTTC Cuts at: 4982 4982 Size: 6853 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4661 276 Size: 53 83 186 936 3127 2468 Fragments arranged by size: 3127 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI