Restriction map for
pFN21ASDA0438
(Linear) (Six-base) MAPSORT of: KIAA0438.seq Check: 1524 from: 1 to: 2124
KIAA0438.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2046 2124
Size: 2046 78
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 1444 1454 1709 2124
Size: 869 575 10 255 415
Fragments arranged by size:
869 575 415 255 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1699 1731 2124
Size: 1699 32 393
Fragments arranged by size:
1699 393 32
ApoI r'AATT_y
Cuts at: 0 222 302 764 1496 1564 2124
Size: 222 80 462 732 68 560
Fragments arranged by size:
732 560 462 222 80 68
BamHI G'GATC_C
Cuts at: 0 1128 1707 2124
Size: 1128 579 417
Fragments arranged by size:
1128 579 417
BbsI GAAGACnn'nnnn_
Cuts at: 0 120 767 1637 1837 2124
Size: 120 647 870 200 287
Fragments arranged by size:
870 647 287 200 120
BciVI GTATCCnnnnn_n'
Cuts at: 0 381 2124
Size: 381 1743
BlpI GC'TnA_GC
Cuts at: 0 1746 2124
Size: 1746 378
BmrI ACTGGGnnnn_n'
Cuts at: 0 263 2124
Size: 263 1861
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 794 1632 2124
Size: 794 838 492
Fragments arranged by size:
838 794 492
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1602 1627 2124
Size: 1602 25 497
Fragments arranged by size:
1602 497 25
Bpu10I CC'TnA_GC
Cuts at: 0 165 1406 2124
Size: 165 1241 718
Fragments arranged by size:
1241 718 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 1125 1341 2124
Size: 764 361 216 783
Fragments arranged by size:
783 764 361 216
BsaI GGTCTCn'nnnn_
Cuts at: 0 1846 2124
Size: 1846 278
BseYI C'CCAG_C
Cuts at: 0 1370 2124
Size: 1370 754
BsmI GAATG_Cn'
Cuts at: 0 1294 2124
Size: 1294 830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1563 2046 2124
Size: 1563 483 78
Fragments arranged by size:
1563 483 78
BsrDI GCAATG_nn'
Cuts at: 0 38 2124
Size: 38 2086
BstXI CCAn_nnnn'nTGG
Cuts at: 0 46 243 2124
Size: 46 197 1881
Fragments arranged by size:
1881 197 46
BstYI r'GATC_y
Cuts at: 0 1128 1707 2124
Size: 1128 579 417
Fragments arranged by size:
1128 579 417
Bsu36I CC'TnA_GG
Cuts at: 0 228 393 2124
Size: 228 165 1731
Fragments arranged by size:
1731 228 165
BtgI C'CryG_G
Cuts at: 0 915 2124
Size: 915 1209
BtsI GCAGTG_nn'
Cuts at: 0 1299 1431 1542 1799 1874 1920 2124
Size: 1299 132 111 257 75 46 204
Fragments arranged by size:
1299 257 204 132 111 75 46
ClaI AT'CG_AT
Cuts at: 0 1326 2124
Size: 1326 798
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 959 994 2124
Size: 959 35 1130
Fragments arranged by size:
1130 959 35
DraI TTT'AAA
Cuts at: 0 135 2124
Size: 135 1989
EaeI y'GGCC_r
Cuts at: 0 1808 2124
Size: 1808 316
EarI CTCTTCn'nnn_
Cuts at: 0 686 1463 1481 2124
Size: 686 777 18 643
Fragments arranged by size:
777 686 643 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 1444 1454 1602 1627 1709 2124
Size: 869 575 10 148 25 82 415
Fragments arranged by size:
869 575 415 148 82 25 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 137 2124
Size: 137 1987
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 1339 1371 2124
Size: 28 32 1279 32 753
Fragments arranged by size:
1279 753 32 32 28
HindIII A'AGCT_T
Cuts at: 0 162 2124
Size: 162 1962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 590 1568 2124
Size: 176 414 978 556
Fragments arranged by size:
978 556 414 176
MscI TGG'CCA
Cuts at: 0 1810 2124
Size: 1810 314
MslI CAynn'nnrTG
Cuts at: 0 122 371 2124
Size: 122 249 1753
Fragments arranged by size:
1753 249 122
MspA1I CmG'CkG
Cuts at: 0 1204 2124
Size: 1204 920
NcoI C'CATG_G
Cuts at: 0 915 2124
Size: 915 1209
NdeI CA'TA_TG
Cuts at: 0 1701 2124
Size: 1701 423
NspI r_CATG'y
Cuts at: 0 121 2012 2124
Size: 121 1891 112
Fragments arranged by size:
1891 121 112
PflMI CCAn_nnn'nTGG
Cuts at: 0 1758 2124
Size: 1758 366
PfoI T'CCnGG_A
Cuts at: 0 441 2124
Size: 441 1683
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1394 1426 2124
Size: 1394 32 698
Fragments arranged by size:
1394 698 32
PstI C_TGCA'G
Cuts at: 0 555 2061 2124
Size: 555 1506 63
Fragments arranged by size:
1506 555 63
SfcI C'TryA_G
Cuts at: 0 551 2057 2124
Size: 551 1506 67
Fragments arranged by size:
1506 551 67
SmlI C'TyrA_G
Cuts at: 0 779 1140 1356 2124
Size: 779 361 216 768
Fragments arranged by size:
779 768 361 216
StyI C'CwwG_G
Cuts at: 0 915 1481 1501 2124
Size: 915 566 20 623
Fragments arranged by size:
915 623 566 20
TatI w'GTAC_w
Cuts at: 0 8 363 403 1511 2124
Size: 8 355 40 1108 613
Fragments arranged by size:
1108 613 355 40 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1227 1259 2124
Size: 1227 32 865
Fragments arranged by size:
1227 865 32
Enzymes that do cut:
AarI AcuI AloI ApoI BamHI BbsI BciVI BlpI
BmrI BplI BpmI Bpu10I BpuEI BsaI BseYI BsmI
BspMI BsrDI BstXI BstYI Bsu36I BtgI BtsI ClaI
CspCI DraI EaeI EarI Eco57MI FalI Hin4I HindIII
MmeI MscI MslI MspA1I NcoI NdeI NspI PflMI
PfoI PsrI PstI SfcI SmlI StyI TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BanII BbeI BbvCI
BcgI BclI BfrBI BglI BglII Bme1580I BmgBI BmtI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI