Restriction map for
pFN21ASDA0396
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AflIII AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AviII AvrII
BamHI BbeI BbrPI BbsI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BstAPI BstBI BstEII BstPI
BstUI CelII ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 1513 GACGTC GACGT^C
AccI 22 GTATAC GT^[AC][GT]AC
AcsI 1075 GAATTT [GA]^AATT[CT]
AgeI 77 ACCGGT A^CCGGT
AocI 931 CCTCAGG CC^T[ACGT]AGG
Aor51HI 134 AGCGCT AGC^GCT
ApoI 1075 GAATTT [GA]^AATT[CT]
AspHI 1688 GAGCTC G[AT]GC[AT]^C
AvaI 1805 CCCGAG C^[CT]CG[GA]G
BalI 648 TGGCCA TGG^CCA
BanII 1688 GAGCTC G[GA]GC[CT]^C
BbvCI 1567 GCTGAGG CCTCAGC(-5/-2)
BcnI 207 CCGGG CC^[GC]GG
BglI 8 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1409 AGATCT A^GATCT
BsaWI 77 ACCGGT [AT]^CCGG[AT]
BseRI 1049 GAGGAG GAGGAG(10/8)
BsiEI 732 CGACCG CG[GA][CT]^CG
BsiHKAI 1688 GAGCTC G[AT]GC[AT]^C
BsmAI 1787 GTCTC GTCTC(1/5)
BsmFI 492 GGGAC GGGAC(10/14)
BsmI 1575 GAATGC GAATGC(1/-1)
BsoBI 1805 CCCGAG C^[CT]CG[GA]G
BspMI 466 ACCTGC ACCTGC(4/8)
BsrFI 77 ACCGGT [GA]^CCGG[CT]
BssSI 1442 CTCGTG CACGAG(-5/-1)
Bst1107I 22 GTATAC GTA^TAC
BstXI 897 CCATGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 22 GTATAC GTA^TAC
Bsu36I 931 CCTCAGG CC^T[ACGT]AGG
CfoI 135 GCGC GCG^C
Cfr10I 77 ACCGGT [GA]^CCGG[CT]
DraIII 551 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 244 GACGGGTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 134 AGCGCT AGC^GCT
Eco81I 931 CCTCAGG CC^T[ACGT]AGG
HaeII 134 AGCGCT [GA]GCGC^[CT]
HgaI 660 GACGC GACGC(5/10)
HhaI 135 GCGC GCG^C
HinP1I 135 GCGC G^CGC
HincII 1474 GTCAAC GT[CT]^[GA]AC
HindII 1474 GTCAAC GT[CT]^[GA]AC
HindIII 752 AAGCTT A^AGCTT
MluNI 648 TGGCCA TGG^CCA
MscI 648 TGGCCA TGG^CCA
NciI 207 CCGGG CC^[GC]GG
NdeI 973 CATATG CA^TATG
NspI 382 GCATGC [GA]CATG^[CT]
PinAI 77 ACCGGT A^CCGGT
PstI 1518 CTGCAG CTGCA^G
SacI 1688 GAGCTC GAGCT^C
SapI 967 GCTCTTC GCTCTTC(1/4)
SphI 382 GCATGC GCATG^C
StuI 981 AGGCCT AGG^CCT
TfiI 1422 GATTC G^A[AT]TC
Tsp45I 1540 GTCAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 660 GACGCC G[GA]^CG[CT]C
1513 GACGTC
AlwNI 934 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
1286 CAGATGCTG
BsaHI 660 GACGCC G[GA]^CG[CT]C
1513 GACGTC
BsiYI 368 CCCCCCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
369 CCCCCTGCGGG
BslI 368 CCCCCCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
369 CCCCCTGCGGG
BstNI 9 CCTGG CC^[AT]GG
1797 CCTGG
DraII 5 AGGGCCT [GA]G^G[ACGT]CC[CT]
1208 AGGCCCC
EarI 968 CTCTTC CTCTTC(1/4)
1861 GAAGAG
EcoO109I 5 AGGGCCT [GA]G^G[ACGT]CC[CT]
1208 AGGCCCC
EcoRII 9 CCTGG ^CC[AT]GG
1797 CCTGG
EcoT14I 327 CCATGG C^C[AT][AT]GG
651 CCATGG
FauI 161 CCCGC CCCGC(4/6)
375 GCGGG
Hin1I 660 GACGCC G[GA]^CG[CT]C
1513 GACGTC
Ksp632I 968 CTCTTC CTCTTC(1/4)
1861 GAAGAG
MspA1I 284 CAGCTG C[AC]G^C[GT]G
581 CAGCTG
MvaI 9 CCTGG CC^[AT]GG
1797 CCTGG
NcoI 327 CCATGG C^CATGG
651 CCATGG
PvuII 284 CAGCTG CAG^CTG
581 CAGCTG
SfcI 635 CTATAG C^T[GA][CT]AG
1518 CTGCAG
SmlI 1166 CTCAAG C^T[CT][GA]AG
1789 CTCAAG
SspI 1018 AATATT AAT^ATT
1279 AATATT
StyI 327 CCATGG C^C[AT][AT]GG
651 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI BanI
BbvI BfaI BmyI BpmI BsaJI
BsgI Bsp1286I BsrDI BsrI BssKI
BstF5I BstYI Cac8I Cfr13I DdeI
DpnII DsaI EaeI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII