(Circular) (Six-base) MAPSORT of: pFN21ASDA0396.seq Check: 8847 from: 1 to: 6610 pFN21ASDA0396 6610 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2498 2498 Size: 6610 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3540 4420 278 Size: 53 83 186 936 2004 880 2468 Fragments arranged by size: 2468 2004 936 880 186 83 53 AccI GT'mk_AC Cuts at: 1937 2046 3953 1937 Size: 109 1907 4594 Fragments arranged by size: 4594 1907 109 Acc65I G'GTAC_C Cuts at: 3931 3931 Size: 6610 AclI AA'CG_TT Cuts at: 1563 4737 5110 1563 Size: 3174 373 3063 Fragments arranged by size: 3174 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2628 4673 6057 6325 1632 Size: 996 2045 1384 268 1917 Fragments arranged by size: 2045 1917 1384 996 268 AfeI AGC'GCT Cuts at: 2159 2159 Size: 6610 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6413 AflIII A'CryG_T Cuts at: 1203 5509 6486 1203 Size: 4306 977 1327 Fragments arranged by size: 4306 1327 977 AgeI A'CCGG_T Cuts at: 2100 5441 2100 Size: 3341 3269 AhdI GACnn_n'nnGTC Cuts at: 5339 5339 Size: 6610 AleI CACnn'nnGTG Cuts at: 1166 2525 1166 Size: 1359 5251 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6610 AlwNI CAG_nnn'CTG Cuts at: 2962 3314 4353 5925 2962 Size: 352 1039 1572 3647 Fragments arranged by size: 3647 1572 1039 352 ApaLI G'TGCA_C Cuts at: 4667 5823 4667 Size: 1156 5454 ApoI r'AATT_y Cuts at: 1493 3098 3919 4145 4297 6406 1493 Size: 1605 821 226 152 2109 1697 Fragments arranged by size: 2109 1697 1605 821 226 152 AseI AT'TA_AT Cuts at: 160 1032 5164 160 Size: 872 4132 1606 Fragments arranged by size: 4132 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6610 AvaI C'yCGr_G Cuts at: 1870 1943 3828 3935 1870 Size: 73 1885 107 4545 Fragments arranged by size: 4545 1885 107 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3756 3789 3756 Size: 33 6577 BamHI G'GATC_C Cuts at: 3940 4307 3940 Size: 367 6243 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2252 2285 2792 3931 5386 Size: 325 217 627 465 33 507 1139 1455 Cuts at: 5386 618 Size: 1842 Fragments arranged by size: 1842 1455 1139 627 507 465 325 217 33 BanII G_rGCy'C Cuts at: 1391 3715 3929 1391 Size: 2324 214 4072 Fragments arranged by size: 4072 2324 214 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5639 BbvCI CC'TCA_GC Cuts at: 3591 3591 Size: 6610 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4801 4835 1903 Size: 34 2864 34 3678 Fragments arranged by size: 3678 2864 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4502 5712 4502 Size: 1210 5400 BclI T'GATC_A Cuts at: 1548 1548 Size: 6610 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2037 5221 136 Size: 107 122 71 631 605 365 3184 1525 Fragments arranged by size: 3184 1525 631 605 365 122 107 71 BglII A'GATC_T Cuts at: 3432 6605 3432 Size: 3173 3437 BlpI GC'TnA_GC Cuts at: 4027 4027 Size: 6610 Bme1580I G_kGCm'C Cuts at: 1790 2619 3628 4671 5827 1790 Size: 829 1009 1043 1156 2573 Fragments arranged by size: 2573 1156 1043 1009 829 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6610 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3777 4341 5299 Size: 416 53 83 399 24 2360 564 958 Cuts at: 5299 442 Size: 1753 Fragments arranged by size: 2360 1753 958 564 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6610 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2331 2984 3495 5270 1239 Size: 1092 653 511 1775 2579 Fragments arranged by size: 2579 1775 1092 653 511 Bpu10I CC'TnA_GC Cuts at: 3591 3591 Size: 6610 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3174 3797 4735 5600 5898 3174 Size: 623 938 865 298 3886 Fragments arranged by size: 3886 938 865 623 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5273 882 Size: 617 3774 2219 Fragments arranged by size: 3774 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3907 4401 493 Size: 711 2703 494 2702 Fragments arranged by size: 2703 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6604 6604 Size: 6610 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2684 3537 4417 4799 Size: 53 83 186 936 1151 853 880 382 Cuts at: 4799 5434 275 Size: 635 1451 Fragments arranged by size: 1451 1151 936 880 853 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2100 5043 5441 5715 5862 6273 2100 Size: 2943 398 274 147 411 2437 Fragments arranged by size: 2943 2437 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2346 2376 1178 Size: 30 165 30 943 30 5412 Fragments arranged by size: 5412 943 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3087 1186 Size: 1901 4709 BseYI C'CCAG_C Cuts at: 2612 5813 6309 2612 Size: 3201 496 2913 Fragments arranged by size: 3201 2913 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2255 2325 3119 3418 1163 Size: 573 519 70 794 299 4355 Fragments arranged by size: 4355 794 573 519 299 70 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2758 4060 4821 5849 1540 Size: 275 186 757 1302 761 1028 2301 Fragments arranged by size: 2301 1302 1028 761 757 275 186 BsiHKAI G_wGCw'C Cuts at: 3715 4671 4756 5827 3715 Size: 956 85 1071 4498 Fragments arranged by size: 4498 1071 956 85 BsmI GAATG_Cn' Cuts at: 3604 4121 4214 3604 Size: 517 93 6000 Fragments arranged by size: 6000 517 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2619 3628 3715 3929 4671 4756 5827 Size: 399 829 1009 87 214 742 85 1071 Cuts at: 5827 1391 Size: 2174 Fragments arranged by size: 2174 1071 1009 829 742 399 214 87 85 BspEI T'CCGG_A Cuts at: 6273 6273 Size: 6610 BspHI T'CATG_A Cuts at: 4499 6229 4499 Size: 1730 4880 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2498 3965 844 Size: 1654 1467 3489 Fragments arranged by size: 3489 1654 1467 BsrBI CCG'CTC Cuts at: 1394 1823 4497 1394 Size: 429 2674 3507 Fragments arranged by size: 3507 2674 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2194 2466 2591 5105 5279 65 Size: 1183 946 272 125 2514 174 1396 Fragments arranged by size: 2514 1396 1183 946 272 174 125 BsrFI r'CCGG_y Cuts at: 1700 1810 2100 5254 5441 1700 Size: 110 290 3154 187 2869 Fragments arranged by size: 3154 2869 290 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6610 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6610 BssSI C'ACGA_G Cuts at: 3465 4670 5682 3465 Size: 1205 1012 4393 Fragments arranged by size: 4393 1205 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6610 BstBI TT'CG_AA Cuts at: 2007 5414 2007 Size: 3407 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6610 BstXI CCAn_nnnn'nTGG Cuts at: 2927 2927 Size: 6610 BstYI r'GATC_y Cuts at: 1972 3172 3432 3867 3940 4307 4692 4709 6161 Size: 1200 260 435 73 367 385 17 1452 Cuts at: 6161 6247 6259 6605 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1200 435 385 367 346 260 86 73 17 12 BstZ17I GTA'TAC Cuts at: 2047 2047 Size: 6610 Bsu36I CC'TnA_GG Cuts at: 2955 2955 Size: 6610 BtgI C'CryG_G Cuts at: 513 1065 2022 2350 2674 3603 513 Size: 552 957 328 324 929 3520 Fragments arranged by size: 3520 957 929 552 328 324 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2161 487 Size: 683 56 935 4936 Fragments arranged by size: 4936 935 683 56 BtsI GCAGTG_nn' Cuts at: 2289 3113 3268 4126 4920 4940 2289 Size: 824 155 858 794 20 3959 Fragments arranged by size: 3959 858 824 794 155 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6610 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6575 DraI TTT'AAA Cuts at: 3914 3914 Size: 6610 DraIII CAC_nnn'GTG Cuts at: 2579 2579 Size: 6610 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2273 5617 809 Size: 507 957 3344 1802 Fragments arranged by size: 3344 1802 957 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2082 2671 Size: 54 1425 50 275 18 225 27 589 Cuts at: 2671 4057 4946 6575 8 Size: 1386 889 1629 43 Fragments arranged by size: 1629 1425 1386 889 589 275 225 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 4057 1537 Size: 275 2245 4090 Fragments arranged by size: 4090 2245 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2997 3879 4540 1353 Size: 1644 882 661 3423 Fragments arranged by size: 3423 1644 882 661 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5197 5571 5717 387 Size: 1064 138 3608 374 146 1280 Fragments arranged by size: 3608 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3713 3713 Size: 6610 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2331 2628 2984 3495 4673 5270 6057 Size: 393 699 297 356 511 1178 597 787 Cuts at: 6057 6325 1239 Size: 268 1524 Fragments arranged by size: 1524 1178 787 699 597 511 393 356 297 268 EcoO109I rG'GnC_Cy Cuts at: 2029 3232 2029 Size: 1203 5407 EcoRI G'AATT_C Cuts at: 3919 4297 3919 Size: 378 6232 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2924 2924 Size: 6610 FspI TGC'GCA Cuts at: 5116 5116 Size: 6610 HaeII r_GCGC'y Cuts at: 1678 2161 5757 1678 Size: 483 3596 2531 Fragments arranged by size: 3596 2531 483 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5251 5283 5325 5357 1373 Size: 32 135 32 3679 32 42 32 2626 Fragments arranged by size: 3679 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3499 3954 4200 669 Size: 988 281 1561 455 246 3079 Fragments arranged by size: 3079 1561 988 455 281 246 HindIII A'AGCT_T Cuts at: 748 2775 748 Size: 2027 4583 HpaI GTT'AAC Cuts at: 4200 4200 Size: 6610 KpnI G_GTAC'C Cuts at: 3935 3935 Size: 6610 MfeI C'AATT_G Cuts at: 4209 4209 Size: 6610 MluI A'CGCG_T Cuts at: 6486 6486 Size: 6610 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2747 3874 5724 5908 1305 Size: 1442 1127 1850 184 2007 Fragments arranged by size: 2007 1850 1442 1127 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2673 10 Size: 54 1425 343 841 3947 Fragments arranged by size: 3947 1425 841 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2523 2525 2925 3216 3531 3722 4568 Size: 648 1357 2 400 291 315 191 846 Cuts at: 4568 4927 5086 518 Size: 359 159 2042 Fragments arranged by size: 2042 1357 846 648 400 359 315 291 191 159 2 MspA1I CmG'CkG Cuts at: 1606 2309 2606 4026 4703 5851 6096 1606 Size: 703 297 1420 677 1148 245 2120 Fragments arranged by size: 2120 1420 1148 703 677 297 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2350 2674 513 Size: 552 957 328 324 4449 Fragments arranged by size: 4449 957 552 328 324 NdeI CA'TA_TG Cuts at: 387 2997 387 Size: 2610 4000 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6610 NotI GC'GGCC_GC Cuts at: 4057 4057 Size: 6610 NspI r_CATG'y Cuts at: 1132 1229 2409 3968 5513 1132 Size: 97 1180 1559 1545 2229 Fragments arranged by size: 2229 1559 1545 1180 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6610 PciI A'CATG_T Cuts at: 5509 5509 Size: 6610 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6610 PmeI GTTT'AAAC Cuts at: 3914 3914 Size: 6610 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2721 2753 4679 4711 6192 6224 2721 Size: 32 1926 32 1481 32 3107 Fragments arranged by size: 3107 1926 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6610 PsiI TTA'TAA Cuts at: 4180 4180 Size: 6610 PstI C_TGCA'G Cuts at: 830 3545 3962 830 Size: 2715 417 3478 Fragments arranged by size: 3478 2715 417 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6610 PvuII CAG'CTG Cuts at: 2309 2606 2309 Size: 297 6313 SacI G_AGCT'C Cuts at: 3715 3715 Size: 6610 SalI G'TCGA_C Cuts at: 1936 3952 1936 Size: 2016 4594 SapI GCTCTTCn'nnn_ Cuts at: 2997 2997 Size: 6610 SbfI CC_TGCA'GG Cuts at: 3962 3962 Size: 6610 ScaI AGT'ACT Cuts at: 4858 6570 4858 Size: 1712 4898 SfcI C'TryA_G Cuts at: 826 1045 2658 3541 3958 5093 5774 5965 826 Size: 219 1613 883 417 1135 681 191 1471 Fragments arranged by size: 1613 1471 1135 883 681 417 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6610 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6610 SmaI CCC'GGG Cuts at: 1872 3937 1872 Size: 2065 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3189 3812 4714 5615 5877 820 Size: 197 926 1246 623 902 901 262 1553 Fragments arranged by size: 1553 1246 926 902 901 623 262 197 SnaBI TAC'GTA Cuts at: 493 3907 4401 493 Size: 3414 494 2702 Fragments arranged by size: 3414 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6610 SphI G_CATG'C Cuts at: 1132 2409 3968 1132 Size: 1277 1559 3774 Fragments arranged by size: 3774 1559 1277 SspI AAT'ATT Cuts at: 5 52 3043 3304 4534 5 Size: 47 2991 261 1230 2081 Fragments arranged by size: 2991 2081 1230 261 47 StuI AGG'CCT Cuts at: 1517 3006 1517 Size: 1489 5121 StyI C'CwwG_G Cuts at: 513 1065 2022 2350 2674 4049 513 Size: 552 957 328 324 1375 3074 Fragments arranged by size: 3074 1375 957 552 328 324 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2772 2780 3179 4637 4654 4807 4992 Size: 1255 1192 8 399 1458 17 153 185 Cuts at: 4992 5437 325 Size: 445 1498 Fragments arranged by size: 1498 1458 1255 1192 445 399 185 153 17 8 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3645 3761 4856 Size: 274 80 33 51 1443 1668 116 1095 Cuts at: 4856 6568 96 Size: 1712 138 Fragments arranged by size: 1712 1668 1443 1095 274 138 116 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2346 2378 3016 3048 6570 6602 2346 Size: 32 638 32 3522 32 2354 Fragments arranged by size: 3522 2354 638 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6610 XbaI T'CTAG_A Cuts at: 3946 3946 Size: 6610 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2029 Size: 6610 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6610 XmaI C'CCGG_G Cuts at: 1870 3935 1870 Size: 2065 4545 XmnI GAAnn'nnTTC Cuts at: 4739 4739 Size: 6610 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3538 4418 276 Size: 53 83 186 936 2004 880 2468 Fragments arranged by size: 2468 2004 936 880 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI