Restriction map for
pFN21ASDA0396
(Linear) (Six-base) MAPSORT of: KIAA0396.seq Check: 5829 from: 1 to: 1881
KIAA0396.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 475 1881
Size: 475 1406
AatII G_ACGT'C
Cuts at: 0 1517 1881
Size: 1517 364
AccI GT'mk_AC
Cuts at: 0 23 1881
Size: 23 1858
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 1881
Size: 605 1276
AfeI AGC'GCT
Cuts at: 0 136 1881
Size: 136 1745
AgeI A'CCGG_T
Cuts at: 0 77 1881
Size: 77 1804
AleI CACnn'nnGTG
Cuts at: 0 502 1881
Size: 502 1379
AlwNI CAG_nnn'CTG
Cuts at: 0 939 1291 1881
Size: 939 352 590
Fragments arranged by size:
939 590 352
ApoI r'AATT_y
Cuts at: 0 1075 1881
Size: 1075 806
AvaI C'yCGr_G
Cuts at: 0 1805 1881
Size: 1805 76
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1733 1766 1881
Size: 1733 33 115
Fragments arranged by size:
1733 115 33
BanI G'GyrC_C
Cuts at: 0 229 262 769 1881
Size: 229 33 507 1112
Fragments arranged by size:
1112 507 229 33
BanII G_rGCy'C
Cuts at: 0 1692 1881
Size: 1692 189
BbvCI CC'TCA_GC
Cuts at: 0 1568 1881
Size: 1568 313
BglI GCCn_nnn'nGGC
Cuts at: 0 14 1881
Size: 14 1867
BglII A'GATC_T
Cuts at: 0 1409 1881
Size: 1409 472
Bme1580I G_kGCm'C
Cuts at: 0 596 1605 1881
Size: 596 1009 276
Fragments arranged by size:
1009 596 276
BmrI ACTGGGnnnn_n'
Cuts at: 0 1754 1881
Size: 1754 127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 961 1472 1881
Size: 308 653 511 409
Fragments arranged by size:
653 511 409 308
Bpu10I CC'TnA_GC
Cuts at: 0 1568 1881
Size: 1568 313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1151 1774 1881
Size: 1151 623 107
Fragments arranged by size:
1151 623 107
BsaHI Gr'CG_yC
Cuts at: 0 661 1514 1881
Size: 661 853 367
Fragments arranged by size:
853 661 367
BsaWI w'CCGG_w
Cuts at: 0 77 1881
Size: 77 1804
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 323 353 1881
Size: 323 30 1528
Fragments arranged by size:
1528 323 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1064 1881
Size: 1064 817
BseYI C'CCAG_C
Cuts at: 0 589 1881
Size: 589 1292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 302 1096 1395 1881
Size: 232 70 794 299 486
Fragments arranged by size:
794 486 299 232 70
BsiEI CG_ry'CG
Cuts at: 0 735 1881
Size: 735 1146
BsiHKAI G_wGCw'C
Cuts at: 0 1692 1881
Size: 1692 189
BsmI GAATG_Cn'
Cuts at: 0 1581 1881
Size: 1581 300
Bsp1286I G_dGCh'C
Cuts at: 0 596 1605 1692 1881
Size: 596 1009 87 189
Fragments arranged by size:
1009 596 189 87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 475 1881
Size: 475 1406
BsrDI GCAATG_nn'
Cuts at: 0 171 443 568 1881
Size: 171 272 125 1313
Fragments arranged by size:
1313 272 171 125
BsrFI r'CCGG_y
Cuts at: 0 77 1881
Size: 77 1804
BssSI C'ACGA_G
Cuts at: 0 1442 1881
Size: 1442 439
BstXI CCAn_nnnn'nTGG
Cuts at: 0 904 1881
Size: 904 977
BstYI r'GATC_y
Cuts at: 0 1149 1409 1844 1881
Size: 1149 260 435 37
Fragments arranged by size:
1149 435 260 37
BstZ17I GTA'TAC
Cuts at: 0 24 1881
Size: 24 1857
Bsu36I CC'TnA_GG
Cuts at: 0 932 1881
Size: 932 949
BtgI C'CryG_G
Cuts at: 0 327 651 1580 1881
Size: 327 324 929 301
Fragments arranged by size:
929 327 324 301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 138 1881
Size: 138 1743
BtsI GCAGTG_nn'
Cuts at: 0 266 1090 1245 1881
Size: 266 824 155 636
Fragments arranged by size:
824 636 266 155
DraIII CAC_nnn'GTG
Cuts at: 0 556 1881
Size: 556 1325
DrdI GACnn_nn'nnGTC
Cuts at: 0 250 1881
Size: 250 1631
EaeI y'GGCC_r
Cuts at: 0 32 59 648 1881
Size: 32 27 589 1233
Fragments arranged by size:
1233 589 32 27
EarI CTCTTCn'nnn_
Cuts at: 0 974 1856 1881
Size: 974 882 25
Fragments arranged by size:
974 882 25
EcoICRI GAG'CTC
Cuts at: 0 1690 1881
Size: 1690 191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 605 961 1472 1881
Size: 308 297 356 511 409
Fragments arranged by size:
511 409 356 308 297
EcoO109I rG'GnC_Cy
Cuts at: 0 6 1209 1881
Size: 6 1203 672
Fragments arranged by size:
1203 672 6
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 901 1881
Size: 901 980
HaeII r_GCGC'y
Cuts at: 0 138 1881
Size: 138 1743
HincII GTy'rAC
Cuts at: 0 1476 1881
Size: 1476 405
HindIII A'AGCT_T
Cuts at: 0 752 1881
Size: 752 1129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1851 1881
Size: 724 1127 30
Fragments arranged by size:
1127 724 30
MscI TGG'CCA
Cuts at: 0 650 1881
Size: 650 1231
MslI CAynn'nnrTG
Cuts at: 0 500 502 902 1193 1508 1699 1881
Size: 500 2 400 291 315 191 182
Fragments arranged by size:
500 400 315 291 191 182 2
MspA1I CmG'CkG
Cuts at: 0 286 583 1881
Size: 286 297 1298
Fragments arranged by size:
1298 297 286
NcoI C'CATG_G
Cuts at: 0 327 651 1881
Size: 327 324 1230
Fragments arranged by size:
1230 327 324
NdeI CA'TA_TG
Cuts at: 0 974 1881
Size: 974 907
NspI r_CATG'y
Cuts at: 0 386 1881
Size: 386 1495
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 698 730 1881
Size: 698 32 1151
Fragments arranged by size:
1151 698 32
PstI C_TGCA'G
Cuts at: 0 1522 1881
Size: 1522 359
PvuII CAG'CTG
Cuts at: 0 286 583 1881
Size: 286 297 1298
Fragments arranged by size:
1298 297 286
SacI G_AGCT'C
Cuts at: 0 1692 1881
Size: 1692 189
SapI GCTCTTCn'nnn_
Cuts at: 0 974 1881
Size: 974 907
SfcI C'TryA_G
Cuts at: 0 635 1518 1881
Size: 635 883 363
Fragments arranged by size:
883 635 363
SmlI C'TyrA_G
Cuts at: 0 1166 1789 1881
Size: 1166 623 92
Fragments arranged by size:
1166 623 92
SphI G_CATG'C
Cuts at: 0 386 1881
Size: 386 1495
SspI AAT'ATT
Cuts at: 0 1020 1281 1881
Size: 1020 261 600
Fragments arranged by size:
1020 600 261
StuI AGG'CCT
Cuts at: 0 983 1881
Size: 983 898
StyI C'CwwG_G
Cuts at: 0 327 651 1881
Size: 327 324 1230
Fragments arranged by size:
1230 327 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 749 757 1156 1881
Size: 749 8 399 725
Fragments arranged by size:
749 725 399 8
TatI w'GTAC_w
Cuts at: 0 1622 1738 1881
Size: 1622 116 143
Fragments arranged by size:
1622 143 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 323 355 993 1025 1881
Size: 323 32 638 32 856
Fragments arranged by size:
856 638 323 32 32
ZraI GAC'GTC
Cuts at: 0 1515 1881
Size: 1515 366
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AgeI AleI AlwNI
ApoI AvaI BaeI BanI BanII BbvCI BglI BglII
Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BsrFI BssSI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoO109I FalI HaeII HincII HindIII MmeI MscI MslI
MspA1I NcoI NdeI NspI PpiI PstI PvuII SacI
SapI SfcI SmlI SphI SspI StuI StyI TaqII
TatI TstI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AhdI AlfI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BamHI BbeI BbsI
BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BplI
BsaI BsaAI BsaBI BsiWI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BstAPI BstBI BstEII ClaI CspCI DraI
EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
Hin4I HpaI KasI KpnI MfeI MluI NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI