Restriction map for
pFN21ASDA0307
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI Aor51HI ApaI AscI
AseI AsnI Asp718I AvaI AviII
AvrII BbeI BbrPI BbvCI BclI
BfaI BfrI BlnI BsaAI BsaHI
BsaI BsiEI BsiWI BsoBI BsrDI
BssHII Bst1107I BstAPI BstBI BstZ17I
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoRV
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MluI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccIII 109 TCCGGA T^CCGGA
AflIII 1643 ACATGT A^C[GA][CT]GT
AhdI 1689 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1093 CCTGAGG CC^T[ACGT]AGG
AspEI 1689 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1017 GGATCC G^GATCC
BanI 963 GGTGCC G^G[CT][GA]CC
BcgI 2076 CGAAGACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 817 GGATAC GTATCC(6/5)
BglI 40 GCCCCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1526 AGATCT A^GATCT
BlpI 1499 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1499 GCTCAGC GC^T[ACGT]AGC
BseAI 109 TCCGGA T^CCGGA
BseRI 1830 CTCCTC GAGGAG(10/8)
BsgI 1263 CTGCAC GTGCAG(16/14)
BsmBI 294 CGTCTC CGTCTC(1/5)
BsmI 1845 GAATGC GAATGC(1/-1)
Bsp1407I 811 TGTACA T^GTACA
BspDI 1169 ATCGAT AT^CGAT
BspEI 109 TCCGGA T^CCGGA
BspHI 1386 TCATGA T^CATGA
BspLU11I 1643 ACATGT A^CATGT
BspMI 1128 GCAGGT ACCTGC(4/8)
BsrBI 1971 GAGCGG CCGCTC(-3/-3)
BsrFI 1378 GCCGGT [GA]^CCGG[CT]
BsrGI 811 TGTACA T^GTACA
BssSI 898 CTCGTG CACGAG(-5/-1)
BstEII 921 GGTGACC G^GT[ACGT]ACC
BstPI 921 GGTGACC G^GT[ACGT]ACC
Bsu36I 1093 CCTGAGG CC^T[ACGT]AGG
CelII 1499 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1378 GCCGGT [GA]^CCGG[CT]
ClaI 1169 ATCGAT AT^CGAT
Eam1105I 1689 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1093 CCTGAGG CC^T[ACGT]AGG
EcoO65I 921 GGTGACC G^GT[ACGT]ACC
EcoRI 1081 GAATTC G^AATTC
EcoT22I 939 ATGCAT ATGCA^T
HgaI 865 GACGC GACGC(5/10)
MfeI 905 CAATTG C^AATTG
MroI 109 TCCGGA T^CCGGA
MseI 1136 TTAA T^TAA
MspA1I 401 CAGCTG C[AC]G^C[GT]G
MunI 905 CAATTG C^AATTG
NsiI 939 ATGCAT ATGCA^T
PflMI 1125 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1355 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 401 CAGCTG CAG^CTG
RcaI 1386 TCATGA T^CATGA
SapI 1580 GCTCTTC GCTCTTC(1/4)
SspBI 811 TGTACA T^GTACA
SspI 890 AATATT AAT^ATT
Tru9I 1136 TTAA T^TAA
Van91I 1125 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 278 CCATCCTCCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
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AccII 345 CGCG CG^CG
665 CGCG
AcsI 151 AAATTT [GA]^AATT[CT]
1081 GAATTC
Alw44I 564 GTGCAC G^TGCAC
1315 GTGCAC
AlwNI 373 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
1502 CAGCCTCTG
ApaLI 564 GTGCAC G^TGCAC
1315 GTGCAC
ApoI 151 AAATTT [GA]^AATT[CT]
1081 GAATTC
Asp700I 747 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1569 GAAGGCCTTC
BalI 832 TGGCCA TGG^CCA
1962 TGGCCA
BsaBI 1197 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1331 GATTGTCATC
BsaWI 55 ACCGGA [AT]^CCGG[AT]
109 TCCGGA
BstUI 345 CGCG CG^CG
665 CGCG
DraII 141 AGGCCCC [GA]G^G[ACGT]CC[CT]
1355 AGGTCCC
EcoO109I 141 AGGCCCC [GA]G^G[ACGT]CC[CT]
1355 AGGTCCC
FauI 73 GCGGG CCCGC(4/6)
1904 GCGGG
MamI 1197 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1331 GATTGTCATC
MluNI 832 TGGCCA TGG^CCA
1962 TGGCCA
MscI 832 TGGCCA TGG^CCA
1962 TGGCCA
MvnI 345 CGCG CG^CG
665 CGCG
NcoI 44 CCATGG C^CATGG
1959 CCATGG
PstI 681 CTGCAG CTGCA^G
1508 CTGCAG
SacI 220 GAGCTC GAGCT^C
1497 GAGCTC
StuI 827 AGGCCT AGG^CCT
1571 AGGCCT
XmnI 747 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1569 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AspI AvaII BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII