(Circular) (Six-base) MAPSORT of: pFN21ASDA0307.seq Check: 5797 from: 1 to: 6847 pFN21ASDA0307 6847 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3142 3142 Size: 6847 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4657 278 Size: 53 83 186 936 3121 2468 Fragments arranged by size: 3121 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4190 1937 Size: 2253 4594 Acc65I G'GTAC_C Cuts at: 4168 4168 Size: 6847 AclI AA'CG_TT Cuts at: 1563 4974 5347 1563 Size: 3411 373 3063 Fragments arranged by size: 3411 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2013 2423 2648 2901 3296 3583 3591 4053 Size: 381 410 225 253 395 287 8 462 Cuts at: 4053 4910 6294 6562 1632 Size: 857 1384 268 1917 Fragments arranged by size: 1917 1384 857 462 410 395 381 287 268 253 225 8 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6650 AflIII A'CryG_T Cuts at: 1203 3666 5746 6723 1203 Size: 2463 2080 977 1327 Fragments arranged by size: 2463 2080 1327 977 AgeI A'CCGG_T Cuts at: 5678 5678 Size: 6847 AhdI GACnn_n'nnGTC Cuts at: 3717 5576 3717 Size: 1859 4988 AleI CACnn'nnGTG Cuts at: 1166 3035 1166 Size: 1869 4978 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6847 AlwNI CAG_nnn'CTG Cuts at: 2401 3530 4590 6162 2401 Size: 1129 1060 1572 3086 Fragments arranged by size: 3086 1572 1129 1060 ApaLI G'TGCA_C Cuts at: 2587 3338 4904 6060 2587 Size: 751 1566 1156 3374 Fragments arranged by size: 3374 1566 1156 751 ApoI r'AATT_y Cuts at: 1493 2174 3104 4156 4382 4534 6643 1493 Size: 681 930 1052 226 152 2109 1697 Fragments arranged by size: 2109 1697 1052 930 681 226 152 AseI AT'TA_AT Cuts at: 160 1032 5401 160 Size: 872 4369 1606 Fragments arranged by size: 4369 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6847 AvaI C'yCGr_G Cuts at: 1870 1943 4172 1870 Size: 73 2229 4545 Fragments arranged by size: 4545 2229 73 BamHI G'GATC_C Cuts at: 3040 4177 4544 3040 Size: 1137 367 5343 Fragments arranged by size: 5343 1137 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2986 4168 5623 618 Size: 325 217 627 1199 1182 1455 1842 Fragments arranged by size: 1842 1455 1199 1182 627 325 217 BanII G_rGCy'C Cuts at: 1391 2247 2687 3524 4166 1391 Size: 856 440 837 642 4072 Fragments arranged by size: 4072 856 837 642 440 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2636 2892 4107 928 Size: 971 737 256 1215 3668 Fragments arranged by size: 3668 1215 971 737 256 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4088 4122 5038 5072 1903 Size: 34 2151 34 916 34 3678 Fragments arranged by size: 3678 2151 916 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2834 4739 5949 2834 Size: 1905 1210 3732 Fragments arranged by size: 3732 1905 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6847 BfrBI ATG'CAT Cuts at: 2964 2964 Size: 6847 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2069 5458 136 Size: 107 122 71 631 605 397 3389 1525 Fragments arranged by size: 3389 1525 631 605 397 122 107 71 BglII A'GATC_T Cuts at: 3549 6842 3549 Size: 3293 3554 BlpI GC'TnA_GC Cuts at: 3523 4264 3523 Size: 741 6106 Bme1580I G_kGCm'C Cuts at: 1790 2591 2991 3342 4908 6064 1790 Size: 801 400 351 1566 1156 2573 Fragments arranged by size: 2573 1566 1156 801 400 351 BmgBI CAC'GTC Cuts at: 1318 2563 1318 Size: 1245 5602 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2163 2506 2643 Size: 416 53 83 399 24 746 343 137 Cuts at: 2643 4017 4578 5536 442 Size: 1374 561 958 1753 Fragments arranged by size: 1753 1374 958 746 561 416 399 343 137 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6847 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4024 4024 Size: 6847 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2924 3645 3823 3862 3882 5507 1239 Size: 1685 721 178 39 20 1625 2579 Fragments arranged by size: 2579 1685 1625 721 178 39 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4972 5837 6135 4972 Size: 865 298 5684 Fragments arranged by size: 5684 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5510 882 Size: 617 4011 2219 Fragments arranged by size: 4011 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4144 4638 493 Size: 711 2940 494 2702 Fragments arranged by size: 2940 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3224 3358 6841 3224 Size: 134 3483 3230 Fragments arranged by size: 3483 3230 134 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4654 5036 5671 275 Size: 53 83 186 936 3121 382 635 1451 Fragments arranged by size: 3121 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2078 2132 5280 5678 5952 6099 6510 2078 Size: 54 3148 398 274 147 411 2415 Fragments arranged by size: 3148 2415 411 398 274 147 54 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2938 2968 3206 3236 1178 Size: 30 165 30 1535 30 238 30 4789 Fragments arranged by size: 4789 1535 238 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3844 1186 Size: 2658 4189 BseYI C'CCAG_C Cuts at: 3543 3844 6050 6546 3543 Size: 301 2206 496 3844 Fragments arranged by size: 3844 2206 496 301 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3271 1163 Size: 573 1535 4739 Fragments arranged by size: 4739 1535 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4297 5058 6086 1540 Size: 275 186 2296 761 1028 2301 Fragments arranged by size: 2301 2296 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2247 2534 2591 3342 3524 4908 4993 6064 2247 Size: 287 57 751 182 1384 85 1071 3030 Fragments arranged by size: 3030 1384 1071 751 287 182 85 57 BsmI GAATG_Cn' Cuts at: 3874 4358 4451 3874 Size: 484 93 6270 Fragments arranged by size: 6270 484 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2323 2323 Size: 6847 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2247 2534 2591 2687 2991 3342 3524 Size: 399 457 287 57 96 304 351 182 Cuts at: 3524 4166 4908 4993 6064 1391 Size: 642 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 642 457 399 351 304 287 182 96 85 57 BspEI T'CCGG_A Cuts at: 2132 6510 2132 Size: 4378 2469 BspHI T'CATG_A Cuts at: 3409 4736 6466 3409 Size: 1327 1730 3790 Fragments arranged by size: 3790 1730 1327 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3142 4202 844 Size: 2298 1060 3489 Fragments arranged by size: 3489 2298 1060 BsrBI CCG'CTC Cuts at: 1394 1823 3996 4734 1394 Size: 429 2173 738 3507 Fragments arranged by size: 3507 2173 738 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5342 5516 65 Size: 1183 4094 174 1396 Fragments arranged by size: 4094 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3401 5491 5678 1700 Size: 110 1591 2090 187 2869 Fragments arranged by size: 2869 2090 1591 187 110 BsrGI T'GTAC_A Cuts at: 96 2834 96 Size: 2738 4109 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6847 BssSI C'ACGA_G Cuts at: 2921 4907 5919 2921 Size: 1986 1012 3849 Fragments arranged by size: 3849 1986 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6847 BstBI TT'CG_AA Cuts at: 2007 5651 2007 Size: 3644 3203 BstEII G'GTnAC_C Cuts at: 1184 2944 1184 Size: 1760 5087 BstXI CCAn_nnnn'nTGG Cuts at: 3350 3983 4082 3350 Size: 633 99 6115 Fragments arranged by size: 6115 633 99 BstYI r'GATC_y Cuts at: 1972 2042 2616 3040 3079 3463 3549 4066 4177 Size: 70 574 424 39 384 86 517 111 Cuts at: 4177 4544 4929 4946 6398 6484 6496 6842 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 574 517 424 385 384 367 346 111 86 86 70 39 17 12 Bsu36I CC'TnA_GG Cuts at: 3117 3117 Size: 6847 BtgI C'CryG_G Cuts at: 513 1065 2022 2067 2113 3426 3654 3982 513 Size: 552 957 45 46 1313 228 328 3378 Fragments arranged by size: 3378 1313 957 552 328 228 46 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6108 Fragments arranged by size: 6108 683 56 BtsI GCAGTG_nn' Cuts at: 3971 4363 5157 5177 3971 Size: 392 794 20 5641 Fragments arranged by size: 5641 794 392 20 ClaI AT'CG_AT Cuts at: 1554 3193 1554 Size: 1639 5208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6812 DraI TTT'AAA Cuts at: 4151 4151 Size: 6847 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5854 809 Size: 507 4538 1802 Fragments arranged by size: 4538 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2313 2855 Size: 54 1425 50 275 18 243 240 542 Cuts at: 2855 3985 4294 5183 6812 8 Size: 1130 309 889 1629 43 Fragments arranged by size: 1629 1425 1130 889 542 309 275 243 240 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4294 1537 Size: 275 2482 4090 Fragments arranged by size: 4090 2482 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3610 3652 3826 4777 1353 Size: 2257 42 174 951 3423 Fragments arranged by size: 3423 2257 951 174 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3452 3714 5434 5808 5954 387 Size: 1064 138 1863 262 1720 374 146 1280 Fragments arranged by size: 1863 1720 1280 1064 374 262 146 138 EcoICRI GAG'CTC Cuts at: 2245 3522 2245 Size: 1277 5570 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2013 2423 2648 2901 2924 3296 3583 Size: 393 381 410 225 253 23 372 287 Cuts at: 3583 3591 3645 3823 3862 3882 4053 4910 5507 Size: 8 54 178 39 20 171 857 597 Cuts at: 5507 6294 6562 1239 Size: 787 268 1524 Fragments arranged by size: 1524 857 787 597 410 393 381 372 287 268 253 225 178 171 54 39 23 20 8 EcoO109I rG'GnC_Cy Cuts at: 2165 3379 2165 Size: 1214 5633 EcoRI G'AATT_C Cuts at: 3104 4156 4534 3104 Size: 1052 378 5417 Fragments arranged by size: 5417 1052 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2398 2398 Size: 6847 FspI TGC'GCA Cuts at: 5353 5353 Size: 6847 HaeII r_GCGC'y Cuts at: 1678 5994 1678 Size: 4316 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2938 2970 2987 3019 3341 Size: 32 135 32 1366 32 17 32 322 Cuts at: 3341 3373 3626 3658 3703 3729 3735 3761 5488 Size: 32 253 32 45 26 6 26 1727 Cuts at: 5488 5520 5562 5594 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1727 1366 322 253 135 45 42 32 32 32 32 32 32 32 32 26 26 17 6 HincII GTy'rAC Cuts at: 669 1657 1938 4191 4437 669 Size: 988 281 2253 246 3079 Fragments arranged by size: 3079 2253 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6847 HpaI GTT'AAC Cuts at: 4437 4437 Size: 6847 KpnI G_GTAC'C Cuts at: 4172 4172 Size: 6847 MfeI C'AATT_G Cuts at: 2928 4446 2928 Size: 1518 5329 MluI A'CGCG_T Cuts at: 6723 6723 Size: 6847 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2274 4094 5961 6145 1305 Size: 969 1820 1867 184 2007 Fragments arranged by size: 2007 1867 1820 969 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2857 3987 10 Size: 54 1425 343 1025 1130 2870 Fragments arranged by size: 2870 1425 1130 1025 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2556 2672 2969 3035 3135 4805 5164 Size: 648 1390 116 297 66 100 1670 359 Cuts at: 5164 5323 518 Size: 159 2042 Fragments arranged by size: 2042 1670 1390 648 359 297 159 116 100 66 MspA1I CmG'CkG Cuts at: 1606 2426 4263 4940 6088 6333 1606 Size: 820 1837 677 1148 245 2120 Fragments arranged by size: 2120 1837 1148 820 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2067 3982 513 Size: 552 957 45 1915 3378 Fragments arranged by size: 3378 1915 957 552 45 NdeI CA'TA_TG Cuts at: 387 387 Size: 6847 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6847 NotI GC'GGCC_GC Cuts at: 4294 4294 Size: 6847 NsiI A_TGCA'T Cuts at: 2966 2966 Size: 6847 NspI r_CATG'y Cuts at: 1132 1229 2268 3670 4051 4123 4205 5750 1132 Size: 97 1039 1402 381 72 82 1545 2229 Fragments arranged by size: 2229 1545 1402 1039 381 97 82 72 PasI CC'CwG_GG Cuts at: 1348 4075 1348 Size: 2727 4120 PciI A'CATG_T Cuts at: 3666 5746 3666 Size: 2080 4767 PflMI CCAn_nnn'nTGG Cuts at: 1108 3154 1108 Size: 2046 4801 PfoI T'CCnGG_A Cuts at: 3587 3733 4041 3587 Size: 146 308 6393 Fragments arranged by size: 6393 308 146 PmeI GTTT'AAAC Cuts at: 4151 4151 Size: 6847 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4916 4948 6429 6461 4916 Size: 32 1481 32 5302 Fragments arranged by size: 5302 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3379 3379 Size: 6847 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6847 PsiI TTA'TAA Cuts at: 4417 4417 Size: 6847 PstI C_TGCA'G Cuts at: 830 2708 3535 4199 830 Size: 1878 827 664 3478 Fragments arranged by size: 3478 1878 827 664 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6847 PvuII CAG'CTG Cuts at: 2426 2426 Size: 6847 SacI G_AGCT'C Cuts at: 2247 3524 2247 Size: 1277 5570 SalI G'TCGA_C Cuts at: 1936 4189 1936 Size: 2253 4594 SapI GCTCTTCn'nnn_ Cuts at: 3610 3610 Size: 6847 SbfI CC_TGCA'GG Cuts at: 4199 4199 Size: 6847 ScaI AGT'ACT Cuts at: 5095 6807 5095 Size: 1712 5135 SfcI C'TryA_G Cuts at: 826 1045 2704 3531 3826 4195 5330 6011 6202 Size: 219 1659 827 295 369 1135 681 191 Cuts at: 6202 826 Size: 1471 Fragments arranged by size: 1659 1471 1135 827 681 369 295 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6847 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6847 SmaI CCC'GGG Cuts at: 1872 4174 1872 Size: 2302 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4951 5852 6114 820 Size: 197 926 3008 901 262 1553 Fragments arranged by size: 3008 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4144 4638 493 Size: 3651 494 2702 Fragments arranged by size: 3651 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6847 SphI G_CATG'C Cuts at: 1132 4205 1132 Size: 3073 3774 SspI AAT'ATT Cuts at: 5 52 2915 4771 5 Size: 47 2863 1856 2081 Fragments arranged by size: 2863 2081 1856 47 StuI AGG'CCT Cuts at: 1517 2852 3596 1517 Size: 1335 744 4768 Fragments arranged by size: 4768 1335 744 StyI C'CwwG_G Cuts at: 513 1065 2022 2067 2788 3559 3937 3982 4286 Size: 552 957 45 721 771 378 45 304 Cuts at: 4286 513 Size: 3074 Fragments arranged by size: 3074 957 771 721 552 378 304 45 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3172 4874 4891 5044 5229 5674 325 Size: 1255 1592 1702 17 153 185 445 1498 Fragments arranged by size: 1702 1592 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2232 2834 3276 Size: 274 80 33 51 1443 255 602 442 Cuts at: 3276 5093 6805 96 Size: 1817 1712 138 Fragments arranged by size: 1817 1712 1443 602 442 274 255 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2315 2347 2701 2733 6807 6839 2315 Size: 32 354 32 4074 32 2323 Fragments arranged by size: 4074 2323 354 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2255 2486 3769 1316 Size: 939 231 1283 4394 Fragments arranged by size: 4394 1283 939 231 XbaI T'CTAG_A Cuts at: 4183 4183 Size: 6847 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2308 2308 Size: 6847 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6847 XmaI C'CCGG_G Cuts at: 1870 4172 1870 Size: 2302 4545 XmnI GAAnn'nnTTC Cuts at: 2774 3596 4976 2774 Size: 822 1380 4645 Fragments arranged by size: 4645 1380 822 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4655 276 Size: 53 83 186 936 3121 2468 Fragments arranged by size: 3121 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI