Restriction map for
pFN21ASDA0307
(Linear) (Six-base) MAPSORT of: KIAA0307.seq Check: 8504 from: 1 to: 2118
KIAA0307.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1119 2118
Size: 1119 999
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 625 878 1273 1560 1568 2030 2118
Size: 400 225 253 395 287 8 462 88
Fragments arranged by size:
462 400 395 287 253 225 88 8
AflIII A'CryG_T
Cuts at: 0 1643 2118
Size: 1643 475
AhdI GACnn_n'nnGTC
Cuts at: 0 1694 2118
Size: 1694 424
AleI CACnn'nnGTG
Cuts at: 0 1012 2118
Size: 1012 1106
AlwNI CAG_nnn'CTG
Cuts at: 0 378 1507 2118
Size: 378 1129 611
Fragments arranged by size:
1129 611 378
ApaLI G'TGCA_C
Cuts at: 0 564 1315 2118
Size: 564 751 803
Fragments arranged by size:
803 751 564
ApoI r'AATT_y
Cuts at: 0 151 1081 2118
Size: 151 930 1037
Fragments arranged by size:
1037 930 151
BamHI G'GATC_C
Cuts at: 0 1017 2118
Size: 1017 1101
BanI G'GyrC_C
Cuts at: 0 963 2118
Size: 963 1155
BanII G_rGCy'C
Cuts at: 0 224 664 1501 2118
Size: 224 440 837 617
Fragments arranged by size:
837 617 440 224
BbsI GAAGACnn'nnnn_
Cuts at: 0 613 869 2084 2118
Size: 613 256 1215 34
Fragments arranged by size:
1215 613 256 34
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2065 2099 2118
Size: 2065 34 19
Fragments arranged by size:
2065 34 19
BciVI GTATCCnnnnn_n'
Cuts at: 0 811 2118
Size: 811 1307
BfrBI ATG'CAT
Cuts at: 0 941 2118
Size: 941 1177
BglI GCCn_nnn'nGGC
Cuts at: 0 46 2118
Size: 46 2072
BglII A'GATC_T
Cuts at: 0 1526 2118
Size: 1526 592
BlpI GC'TnA_GC
Cuts at: 0 1500 2118
Size: 1500 618
Bme1580I G_kGCm'C
Cuts at: 0 568 968 1319 2118
Size: 568 400 351 799
Fragments arranged by size:
799 568 400 351
BmgBI CAC'GTC
Cuts at: 0 540 2118
Size: 540 1578
BmrI ACTGGGnnnn_n'
Cuts at: 0 140 483 620 1994 2118
Size: 140 343 137 1374 124
Fragments arranged by size:
1374 343 140 137 124
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2001 2118
Size: 2001 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1622 1800 1839 1859 2118
Size: 901 721 178 39 20 259
Fragments arranged by size:
901 721 259 178 39 20
BsaBI GATnn'nnATC
Cuts at: 0 1201 1335 2118
Size: 1201 134 783
Fragments arranged by size:
1201 783 134
BsaWI w'CCGG_w
Cuts at: 0 55 109 2118
Size: 55 54 2009
Fragments arranged by size:
2009 55 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 915 945 1183 1213 2118
Size: 915 30 238 30 905
Fragments arranged by size:
915 905 238 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1821 2118
Size: 1821 297
BseYI C'CCAG_C
Cuts at: 0 1520 1821 2118
Size: 1520 301 297
Fragments arranged by size:
1520 301 297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1248 2118
Size: 1248 870
BsiHKAI G_wGCw'C
Cuts at: 0 224 511 568 1319 1501 2118
Size: 224 287 57 751 182 617
Fragments arranged by size:
751 617 287 224 182 57
BsmI GAATG_Cn'
Cuts at: 0 1851 2118
Size: 1851 267
BsmBI CGTCTCn'nnnn_
Cuts at: 0 300 2118
Size: 300 1818
Bsp1286I G_dGCh'C
Cuts at: 0 224 511 568 664 968 1319 1501 2118
Size: 224 287 57 96 304 351 182 617
Fragments arranged by size:
617 351 304 287 224 182 96 57
BspEI T'CCGG_A
Cuts at: 0 109 2118
Size: 109 2009
BspHI T'CATG_A
Cuts at: 0 1386 2118
Size: 1386 732
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1119 2118
Size: 1119 999
BsrBI CCG'CTC
Cuts at: 0 1973 2118
Size: 1973 145
BsrFI r'CCGG_y
Cuts at: 0 1378 2118
Size: 1378 740
BsrGI T'GTAC_A
Cuts at: 0 811 2118
Size: 811 1307
BssSI C'ACGA_G
Cuts at: 0 898 2118
Size: 898 1220
BstEII G'GTnAC_C
Cuts at: 0 921 2118
Size: 921 1197
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1327 1960 2059 2118
Size: 1327 633 99 59
Fragments arranged by size:
1327 633 99 59
BstYI r'GATC_y
Cuts at: 0 19 593 1017 1056 1440 1526 2043 2118
Size: 19 574 424 39 384 86 517 75
Fragments arranged by size:
574 517 424 384 86 75 39 19
Bsu36I CC'TnA_GG
Cuts at: 0 1094 2118
Size: 1094 1024
BtgI C'CryG_G
Cuts at: 0 44 90 1403 1631 1959 2118
Size: 44 46 1313 228 328 159
Fragments arranged by size:
1313 328 228 159 46 44
BtsI GCAGTG_nn'
Cuts at: 0 1948 2118
Size: 1948 170
ClaI AT'CG_AT
Cuts at: 0 1170 2118
Size: 1170 948
EaeI y'GGCC_r
Cuts at: 0 50 290 832 1962 2118
Size: 50 240 542 1130 156
Fragments arranged by size:
1130 542 240 156 50
EarI CTCTTCn'nnn_
Cuts at: 0 1587 1629 1803 2118
Size: 1587 42 174 315
Fragments arranged by size:
1587 315 174 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1429 1691 2118
Size: 1429 262 427
Fragments arranged by size:
1429 427 262
EcoICRI GAG'CTC
Cuts at: 0 222 1499 2118
Size: 222 1277 619
Fragments arranged by size:
1277 619 222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 625 878 901 1273 1560 1568 1622
Size: 400 225 253 23 372 287 8 54
Cuts at: 1622 1800 1839 1859 2030 2118
Size: 178 39 20 171 88
Fragments arranged by size:
400 372 287 253 225 178 171 88
54 39 23 20 8
EcoO109I rG'GnC_Cy
Cuts at: 0 142 1356 2118
Size: 142 1214 762
Fragments arranged by size:
1214 762 142
EcoRI G'AATT_C
Cuts at: 0 1081 2118
Size: 1081 1037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 375 2118
Size: 375 1743
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 915 947 964 996 1318 1350 1603 1635
Size: 915 32 17 32 322 32 253 32
Cuts at: 1635 1680 1706 1712 1738 2118
Size: 45 26 6 26 380
Fragments arranged by size:
915 380 322 253 45 32 32 32
32 26 26 17 6
MfeI C'AATT_G
Cuts at: 0 905 2118
Size: 905 1213
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 2071 2118
Size: 251 1820 47
Fragments arranged by size:
1820 251 47
MscI TGG'CCA
Cuts at: 0 834 1964 2118
Size: 834 1130 154
Fragments arranged by size:
1130 834 154
MslI CAynn'nnrTG
Cuts at: 0 533 649 946 1012 1112 2118
Size: 533 116 297 66 100 1006
Fragments arranged by size:
1006 533 297 116 100 66
MspA1I CmG'CkG
Cuts at: 0 403 2118
Size: 403 1715
NcoI C'CATG_G
Cuts at: 0 44 1959 2118
Size: 44 1915 159
Fragments arranged by size:
1915 159 44
NsiI A_TGCA'T
Cuts at: 0 943 2118
Size: 943 1175
NspI r_CATG'y
Cuts at: 0 245 1647 2028 2100 2118
Size: 245 1402 381 72 18
Fragments arranged by size:
1402 381 245 72 18
PasI CC'CwG_GG
Cuts at: 0 2052 2118
Size: 2052 66
PciI A'CATG_T
Cuts at: 0 1643 2118
Size: 1643 475
PflMI CCAn_nnn'nTGG
Cuts at: 0 1131 2118
Size: 1131 987
PfoI T'CCnGG_A
Cuts at: 0 1564 1710 2018 2118
Size: 1564 146 308 100
Fragments arranged by size:
1564 308 146 100
PpuMI rG'GwC_Cy
Cuts at: 0 1356 2118
Size: 1356 762
PstI C_TGCA'G
Cuts at: 0 685 1512 2118
Size: 685 827 606
Fragments arranged by size:
827 685 606
PvuII CAG'CTG
Cuts at: 0 403 2118
Size: 403 1715
SacI G_AGCT'C
Cuts at: 0 224 1501 2118
Size: 224 1277 617
Fragments arranged by size:
1277 617 224
SapI GCTCTTCn'nnn_
Cuts at: 0 1587 2118
Size: 1587 531
SfcI C'TryA_G
Cuts at: 0 681 1508 1803 2118
Size: 681 827 295 315
Fragments arranged by size:
827 681 315 295
SspI AAT'ATT
Cuts at: 0 892 2118
Size: 892 1226
StuI AGG'CCT
Cuts at: 0 829 1573 2118
Size: 829 744 545
Fragments arranged by size:
829 744 545
StyI C'CwwG_G
Cuts at: 0 44 765 1536 1914 1959 2118
Size: 44 721 771 378 45 159
Fragments arranged by size:
771 721 378 159 45 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1149 2118
Size: 1149 969
TatI w'GTAC_w
Cuts at: 0 209 811 1253 2118
Size: 209 602 442 865
Fragments arranged by size:
865 602 442 209
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 292 324 678 710 2118
Size: 292 32 354 32 1408
Fragments arranged by size:
1408 354 292 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 232 463 1746 2118
Size: 232 231 1283 372
Fragments arranged by size:
1283 372 232 231
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 285 2118
Size: 285 1833
XmnI GAAnn'nnTTC
Cuts at: 0 751 1573 2118
Size: 751 822 545
Fragments arranged by size:
822 751 545
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlwNI ApaLI ApoI
BamHI BanI BanII BbsI BcgI BciVI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssSI
BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I
MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI
PasI PciI PflMI PfoI PpuMI PstI PvuII SacI
SapI SfcI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AlfI
AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BbvCI BclI BmtI Bpu10I BpuEI BsaI BsaAI
BsaHI BsiEI BsiWI BsrDI BssHII BstAPI BstBI BstZ17I
BtgZI CspCI DraI DraIII DrdI EagI EcoNI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SwaI XbaI XhoI XmaI
ZraI