(Circular) (Six-base) MAPSORT of: pFN21ASDA0112.seq Check: 8228 from: 1 to: 5824 pFN21ASDA0112 5824 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3167 1937 Size: 1230 4594 Acc65I G'GTAC_C Cuts at: 3145 3145 Size: 5824 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3887 5271 5539 1632 Size: 2255 1384 268 1917 Fragments arranged by size: 2255 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 4723 5700 1203 Size: 3520 977 1327 Fragments arranged by size: 3520 1327 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 4553 4553 Size: 5824 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5824 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2114 1184 Size: 930 4894 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2516 2548 2516 Size: 32 5792 AlwNI CAG_nnn'CTG Cuts at: 2212 3567 5139 2212 Size: 1355 1572 2897 Fragments arranged by size: 2897 1572 1355 ApaI G_GGCC'C Cuts at: 3002 3002 Size: 5824 ApaLI G'TGCA_C Cuts at: 2099 3881 5037 2099 Size: 1782 1156 2886 Fragments arranged by size: 2886 1782 1156 ApoI r'AATT_y Cuts at: 1493 3049 3133 3359 3511 5620 1493 Size: 1556 84 226 152 2109 1697 Fragments arranged by size: 2109 1697 1556 226 152 84 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 2469 2757 3149 1870 Size: 73 526 288 392 4545 Fragments arranged by size: 4545 526 392 288 73 BamHI G'GATC_C Cuts at: 3154 3521 3154 Size: 367 5457 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2500 2752 3145 4600 618 Size: 325 217 627 713 252 393 1455 1842 Fragments arranged by size: 1842 1455 713 627 393 325 252 217 BanII G_rGCy'C Cuts at: 1391 2307 2765 3002 3143 1391 Size: 916 458 237 141 4072 Fragments arranged by size: 4072 916 458 237 141 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2411 928 Size: 971 512 4341 Fragments arranged by size: 4341 971 512 BbvCI CC'TCA_GC Cuts at: 2862 2862 Size: 5824 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2152 2186 4015 4049 1903 Size: 34 215 34 1829 34 3678 Fragments arranged by size: 3678 1829 215 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2385 2949 4435 Size: 107 122 71 631 605 713 564 1486 Cuts at: 4435 136 Size: 1525 Fragments arranged by size: 1525 1486 713 631 605 564 122 107 71 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 3241 3241 Size: 5824 Bme1580I G_kGCm'C Cuts at: 1790 2103 2505 2757 3002 3885 5041 1790 Size: 313 402 252 245 883 1156 2573 Fragments arranged by size: 2573 1156 883 402 313 252 245 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5824 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3555 4513 442 Size: 416 53 83 399 24 2138 958 1753 Fragments arranged by size: 2138 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 4484 1239 Size: 893 2352 2579 Fragments arranged by size: 2579 2352 893 Bpu10I CC'TnA_GC Cuts at: 2862 2862 Size: 5824 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2366 3949 4814 5112 2366 Size: 1583 865 298 3078 Fragments arranged by size: 3078 1583 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2707 4487 882 Size: 617 1208 1780 2219 Fragments arranged by size: 2219 1780 1208 617 BsaAI yAC'GTr Cuts at: 493 1204 3121 3615 493 Size: 711 1917 494 2702 Fragments arranged by size: 2702 1917 711 494 BsaBI GATnn'nnATC Cuts at: 5818 5818 Size: 5824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2144 3631 4013 4648 Size: 53 83 186 936 611 1487 382 635 Cuts at: 4648 275 Size: 1451 Fragments arranged by size: 1487 1451 936 635 611 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2151 2651 4257 4655 4929 5076 5487 2151 Size: 500 1606 398 274 147 411 2488 Fragments arranged by size: 2488 1606 500 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2105 2135 2516 2546 1178 Size: 30 165 30 702 30 381 30 4456 Fragments arranged by size: 4456 702 381 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2684 2918 2930 2944 3124 1186 Size: 871 627 234 12 14 180 3886 Fragments arranged by size: 3886 871 627 234 180 14 12 BseYI C'CCAG_C Cuts at: 2451 2627 2674 3005 5027 5523 2451 Size: 176 47 331 2022 496 2752 Fragments arranged by size: 2752 2022 496 331 176 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5251 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2166 2345 2637 3274 4035 5063 Size: 275 186 165 179 292 637 761 1028 Cuts at: 5063 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 637 292 275 186 179 165 BsiHKAI G_wGCw'C Cuts at: 2103 3885 3970 5041 2103 Size: 1782 85 1071 2886 Fragments arranged by size: 2886 1782 1071 85 BsmI GAATG_Cn' Cuts at: 3335 3428 3335 Size: 93 5731 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2307 2505 2757 2765 3002 3143 Size: 399 313 204 198 252 8 237 141 Cuts at: 3143 3885 3970 5041 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 313 252 237 204 198 141 85 8 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 2844 3713 5443 2844 Size: 869 1730 3225 Fragments arranged by size: 3225 1730 869 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2145 3179 844 Size: 1301 1034 3489 Fragments arranged by size: 3489 1301 1034 BsrBI CCG'CTC Cuts at: 1394 1823 3711 1394 Size: 429 1888 3507 Fragments arranged by size: 3507 1888 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2515 4319 4493 65 Size: 1183 1267 1804 174 1396 Fragments arranged by size: 1804 1396 1267 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2586 2636 2967 4468 4655 1700 Size: 110 776 50 331 1501 187 2869 Fragments arranged by size: 2869 1501 776 331 187 110 50 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5824 BssHII G'CGCG_C Cuts at: 1925 2375 2464 2607 1925 Size: 450 89 143 5142 Fragments arranged by size: 5142 450 143 89 BssSI C'ACGA_G Cuts at: 3884 4896 3884 Size: 1012 4812 BstAPI GCAn_nnn'nTGC Cuts at: 794 2617 794 Size: 1823 4001 BstBI TT'CG_AA Cuts at: 2007 4628 2007 Size: 2621 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5824 BstXI CCAn_nnnn'nTGG Cuts at: 2692 2692 Size: 5824 BstYI r'GATC_y Cuts at: 1972 3154 3521 3906 3923 5375 5461 5473 5819 Size: 1182 367 385 17 1452 86 12 346 Cuts at: 5819 1972 Size: 1977 Fragments arranged by size: 1977 1452 1182 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2262 2345 513 Size: 552 957 240 83 3992 Fragments arranged by size: 3992 957 552 240 83 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5085 Fragments arranged by size: 5085 683 56 BtsI GCAGTG_nn' Cuts at: 3340 4134 4154 3340 Size: 794 20 5010 Fragments arranged by size: 5010 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5789 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4831 809 Size: 507 3515 1802 Fragments arranged by size: 3515 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2348 2434 2568 Size: 54 1425 50 275 18 518 86 134 Cuts at: 2568 2634 2694 3271 4160 5789 8 Size: 66 60 577 889 1629 43 Fragments arranged by size: 1629 1425 889 577 518 275 134 86 66 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2634 3271 1537 Size: 275 822 637 4090 Fragments arranged by size: 4090 822 637 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2271 3014 3754 1353 Size: 918 743 740 3423 Fragments arranged by size: 3423 918 743 740 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4411 4785 4931 387 Size: 1064 138 2822 374 146 1280 Fragments arranged by size: 2822 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 3887 4484 5271 5539 1239 Size: 393 500 1755 597 787 268 1524 Fragments arranged by size: 1755 1524 787 597 500 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2741 2741 Size: 5824 EcoO109I rG'GnC_Cy Cuts at: 2209 2975 2998 2209 Size: 766 23 5035 Fragments arranged by size: 5035 766 23 EcoRI G'AATT_C Cuts at: 3049 3133 3511 3049 Size: 84 378 5362 Fragments arranged by size: 5362 378 84 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 2452 2725 2737 3097 4971 1678 Size: 774 273 12 360 1874 2531 Fragments arranged by size: 2531 1874 774 360 273 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4465 4497 4539 4571 1373 Size: 32 135 32 2893 32 42 32 2626 Fragments arranged by size: 2893 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3168 3414 669 Size: 988 281 1230 246 3079 Fragments arranged by size: 3079 1230 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 3149 3149 Size: 5824 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2127 2808 4938 5122 1305 Size: 822 681 2130 184 2007 Fragments arranged by size: 2130 2007 822 681 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2436 2570 2696 10 Size: 54 1425 343 604 134 126 3138 Fragments arranged by size: 3138 1425 604 343 134 126 54 MslI CAynn'nnrTG Cuts at: 518 1166 2096 2690 3782 4141 4300 518 Size: 648 930 594 1092 359 159 2042 Fragments arranged by size: 2042 1092 930 648 594 359 159 MspA1I CmG'CkG Cuts at: 1606 2248 2257 2347 2623 2866 3240 3917 5065 Size: 642 9 90 276 243 374 677 1148 Cuts at: 5065 5310 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 642 374 276 245 243 90 9 NaeI GCC'GGC Cuts at: 2638 2969 2638 Size: 331 5493 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4315 Fragments arranged by size: 4315 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NgoMIV G'CCGG_C Cuts at: 2636 2967 2636 Size: 331 5493 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 3182 4727 1132 Size: 97 1953 1545 2229 Fragments arranged by size: 2229 1953 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5824 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 2038 2487 1108 Size: 930 449 4445 Fragments arranged by size: 4445 930 449 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2516 2548 3893 3925 5406 5438 2516 Size: 32 1345 32 1481 32 2902 Fragments arranged by size: 2902 1481 1345 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PspOMI G'GGCC_C Cuts at: 2998 2998 Size: 5824 PstI C_TGCA'G Cuts at: 830 2121 3176 830 Size: 1291 1055 3478 Fragments arranged by size: 3478 1291 1055 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 PvuII CAG'CTG Cuts at: 2248 2257 2623 2866 2248 Size: 9 366 243 5206 Fragments arranged by size: 5206 366 243 9 RsrII CG'GwC_CG Cuts at: 2149 2149 Size: 5824 SacII CC_GC'GG Cuts at: 2348 2348 Size: 5824 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SexAI A'CCwGG_T Cuts at: 2412 2412 Size: 5824 SfcI C'TryA_G Cuts at: 826 1045 2117 2204 3172 4307 4988 5179 826 Size: 219 1072 87 968 1135 681 191 1471 Fragments arranged by size: 1471 1135 1072 968 681 219 191 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 2471 2759 3151 1872 Size: 599 288 392 4545 Fragments arranged by size: 4545 599 392 288 SmlI C'TyrA_G Cuts at: 820 1017 1943 2381 3928 4829 5091 820 Size: 197 926 438 1547 901 262 1553 Fragments arranged by size: 1553 1547 926 901 438 262 197 SnaBI TAC'GTA Cuts at: 493 3121 3615 493 Size: 2628 494 2702 Fragments arranged by size: 2702 2628 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 3182 1132 Size: 2050 3774 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5824 StyI C'CwwG_G Cuts at: 513 1065 2022 2520 2697 2742 3263 513 Size: 552 957 498 177 45 521 3074 Fragments arranged by size: 3074 957 552 521 498 177 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3059 3851 3868 4021 4206 4651 325 Size: 1255 1479 792 17 153 185 445 1498 Fragments arranged by size: 1498 1479 1255 792 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4070 5782 96 Size: 274 80 33 51 1443 2093 1712 138 Fragments arranged by size: 2093 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5784 5816 5784 Size: 32 5792 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2269 2358 2713 2269 Size: 89 355 5380 Fragments arranged by size: 5380 355 89 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 2469 2757 3149 1870 Size: 599 288 392 4545 Fragments arranged by size: 4545 599 392 288 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI SacI SanDI SapI SfiI SfoI SrfI SwaI