Restriction map for
pFN21AB7785
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BfaI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT14I
FauI FseI FspI HaeII HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NlaIV NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SapI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspI
StyI SwaI TfiI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 512 GTCGAC GT^[AC][GT]AC
AlwI 372 GGATC GGATC(4/5)
AspHI 1272 GAGCTC G[AT]GC[AT]^C
BanII 1272 GAGCTC G[GA]GC[CT]^C
BbvI 387 GCTGC GCAGC(8/12)
BclI 305 TGATCA T^GATCA
BglII 219 AGATCT A^GATCT
BmyI 1272 GAGCTC G[AGT]GC[ACT]^C
BsaI 126 GGTCTC GGTCTC(1/5)
BseRI 52 GAGGAG GAGGAG(10/8)
BsiHKAI 1272 GAGCTC G[AT]GC[AT]^C
BsmI 904 GAATGC GAATGC(1/-1)
Bsp1286I 1272 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 89 TGTACA T^GTACA
BsrGI 89 TGTACA T^GTACA
BstXI 489 CCATGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 219 AGATCT [GA]^GATC[CT]
DraI 349 TTTAAA TTT^AAA
EarI 1128 GAAGAG CTCTTC(1/4)
FbaI 305 TGATCA T^GATCA
Fnu4HI 387 GCTGC GC^[ACGT]GC
HapII 1185 CCGG C^CGG
HgaI 515 GACGC GACGC(5/10)
HincII 512 GTCGAC GT[CT]^[GA]AC
HindII 512 GTCGAC GT[CT]^[GA]AC
HindIII 1007 AAGCTT A^AGCTT
HpaII 1185 CCGG C^CGG
ItaI 387 GCTGC GC^[ACGT]GC
Ksp632I 1128 GAAGAG CTCTTC(1/4)
MboII 1128 GAAGA GAAGA(8/7)
MflI 219 AGATCT [GA]^GATC[CT]
MspA1I 1266 CAGCTG C[AC]G^C[GT]G
MspI 1185 CCGG C^CGG
MwoI 359 GCTGGAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PvuII 1266 CAGCTG CAG^CTG
SacI 1272 GAGCTC GAGCT^C
SalI 512 GTCGAC G^TCGAC
ScaI 463 AGTACT AGT^ACT
SphI 1104 GCATGC GCATG^C
SspBI 89 TGTACA T^GTACA
StuI 755 AGGCCT AGG^CCT
TseI 387 GCTGC G^C[AT]GC
XhoII 219 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BalI 32 TGGCCA TGG^CCA
117 TGGCCA
Cac8I 72 GCCTGC GC[ACGT]^[ACGT]GC
1104 GCATGC
Cfr13I 331 GGCCC G^G[ACGT]CC
476 GGCCC
DraII 330 AGGCCCT [GA]G^G[ACGT]CC[CT]
475 AGGCCCT
EaeI 32 TGGCCA [CT]^GGCC[GA]
117 TGGCCA
EcoO109I 330 AGGCCCT [GA]G^G[ACGT]CC[CT]
475 AGGCCCT
HphI 247 GGTGA GGTGA(8/7)
265 TCACC
MluNI 32 TGGCCA TGG^CCA
117 TGGCCA
MscI 32 TGGCCA TGG^CCA
117 TGGCCA
MslI 490 CATGAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
563 CATATGCATG
NdeI 451 CATATG CA^TATG
563 CATATG
NspI 568 GCATGT [GA]CATG^[CT]
1104 GCATGC
Sau96I 331 GGCCC G^G[ACGT]CC
476 GGCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BpmI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI DdeI
DpnII EcoRI EcoRII EcoT22I FokI
HaeIII HinfI MaeIII MboI MnlI
MseI MvaI NdeII NlaIII NsiI
PleI RsaI Sau3AI ScrFI SfaNI
TaqI Tru9I Tsp45I Tsp509I TspRI
TthHB8I XmnI