Restriction map for
pFN21AB9348
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflII AflIII AgeI Alw44I
AlwI AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII AvrII BamHI
BbrPI BbsI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
EagI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MflI MluI MseI MslI
MspA1I MvnI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccIII 462 TCCGGA T^CCGGA
AcyI 671 GGCGCC G[GA]^CG[CT]C
AhdI 411 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 10 CCTGAGG CC^T[ACGT]AGG
ApaI 811 GGGCCC GGGCC^C
AspEI 411 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 49 TGGCCA TGG^CCA
BanI 671 GGCGCC G^G[CT][GA]CC
BbeI 671 GGCGCC GGCGC^C
BbvCI 535 GCTGAGG CCTCAGC(-5/-2)
BcgI 776 GCAAGACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 563 TGATCA T^GATCA
BfaI 292 CTAG C^TAG
BsaBI 690 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 671 GGCGCC G[GA]^CG[CT]C
BsaI 799 GAGACC GGTCTC(1/5)
BsaWI 462 TCCGGA [AT]^CCGG[AT]
BseAI 462 TCCGGA T^CCGGA
BspEI 462 TCCGGA T^CCGGA
BspMI 269 ACCTGC ACCTGC(4/8)
BstAPI 36 GCACTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 10 CCTGAGG CC^T[ACGT]AGG
CfoI 672 GCGC GCG^C
DrdI 780 GACCATCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 122 CCATGG C^C[GA][CT]GG
Eam1105I 411 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 10 CCTGAGG CC^T[ACGT]AGG
FbaI 563 TGATCA T^GATCA
HaeII 671 GGCGCC [GA]GCGC^[CT]
HgaI 626 GACGC GACGC(5/10)
HhaI 672 GCGC GCG^C
Hin1I 671 GGCGCC G[GA]^CG[CT]C
HinP1I 672 GCGC G^CGC
HphI 692 TCACC GGTGA(8/7)
KasI 671 GGCGCC G^GCGCC
MaeI 292 CTAG C^TAG
MaeII 641 ACGT A^CGT
MaeIII 657 GTCAC ^GT[ACGT]AC
MamI 690 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 432 CAATTG C^AATTG
MluNI 49 TGGCCA TGG^CCA
MroI 462 TCCGGA T^CCGGA
MscI 49 TGGCCA TGG^CCA
MunI 432 CAATTG C^AATTG
NarI 671 GGCGCC GG^CGCC
NcoI 122 CCATGG C^CATGG
NdeI 173 CATATG CA^TATG
NspI 5 ACATGC [GA]CATG^[CT]
PflMI 172 CCATATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 505 GAGCTC GAGCT^C
ScaI 614 AGTACT AGT^ACT
SexAI 476 ACCTGGT A^CC[AT]GGT
SfcI 281 CTGTAG C^T[GA][CT]AG
SfoI 671 GGCGCC GGC^GCC
SmlI 19 CTCAAG C^T[CT][GA]AG
TaqI 425 TCGA T^CGA
Tsp45I 657 GTCAC ^GT[GC]AC
Tsp509I 433 AATT ^AATT
TthHB8I 425 TCGA T^CGA
Van91I 172 CCATATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 201 GTAC GT^AC
615 GTAC
BanII 505 GAGCTC G[GA]GC[CT]^C
811 GGGCCC
BcnI 668 CCCGG CC^[GC]GG
699 CCGGG
DpnII 564 GATC ^GATC
690 GATC
EaeI 49 TGGCCA [CT]^GGCC[GA]
746 TGGCCG
EcoNI 393 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
711 CCTGGAGAAGG
EcoT14I 46 CCTTGG C^C[AT][AT]GG
122 CCATGG
MboI 564 GATC ^GATC
690 GATC
NciI 668 CCCGG CC^[GC]GG
699 CCGGG
NdeII 564 GATC ^GATC
690 GATC
PleI 411 GACTC GAGTC(4/5)
304 GAGTC
PpuMI 14 AGGTCCT [GA]G^G[AT]CC[CT]
542 AGGACCT
RsaI 201 GTAC GT^AC
615 GTAC
SapI 314 GCTCTTC GCTCTTC(1/4)
507 GCTCTTC
Sau3AI 564 GATC ^GATC
690 GATC
StyI 46 CCTTGG C^C[AT][AT]GG
122 CCATGG
TspRI 345 CACTG CA[GC]TG[ACGT][ACGT]^
489 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AspHI AvaII BbvI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DraII EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MboII MnlI MspI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfaNI TfiI TseI