Restriction map for
pFN21AB9348
(Linear) (Six-base) MAPSORT of: KIBB9348.seq Check: 9815 from: 1 to: 819
KIBB9348.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 819
Size: 568 251
AhdI GACnn_n'nnGTC
Cuts at: 0 416 819
Size: 416 403
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 548 819
Size: 548 271
ApaI G_GGCC'C
Cuts at: 0 815 819
Size: 815 4
BanI G'GyrC_C
Cuts at: 0 671 819
Size: 671 148
BanII G_rGCy'C
Cuts at: 0 509 815 819
Size: 509 306 4
Fragments arranged by size:
509 306 4
BbeI G_GCGC'C
Cuts at: 0 675 819
Size: 675 144
BbvCI CC'TCA_GC
Cuts at: 0 536 819
Size: 536 283
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 765 799 819
Size: 765 34 20
Fragments arranged by size:
765 34 20
BclI T'GATC_A
Cuts at: 0 563 819
Size: 563 256
Bme1580I G_kGCm'C
Cuts at: 0 815 819
Size: 815 4
BmrI ACTGGGnnnn_n'
Cuts at: 0 410 819
Size: 410 409
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 428 819
Size: 428 391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 112 217 247 415 471 733 819
Size: 52 60 105 30 168 56 262 86
Fragments arranged by size:
262 168 105 86 60 56 52 30
Bpu10I CC'TnA_GC
Cuts at: 0 536 819
Size: 536 283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 819
Size: 4 815
BsaI GGTCTCn'nnnn_
Cuts at: 0 793 819
Size: 793 26
BsaBI GATnn'nnATC
Cuts at: 0 694 819
Size: 694 125
BsaHI Gr'CG_yC
Cuts at: 0 672 819
Size: 672 147
BsaWI w'CCGG_w
Cuts at: 0 462 819
Size: 462 357
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 330 553 819
Size: 109 221 223 266
Fragments arranged by size:
266 223 221 109
BseYI C'CCAG_C
Cuts at: 0 554 819
Size: 554 265
BsiHKAI G_wGCw'C
Cuts at: 0 38 485 509 819
Size: 38 447 24 310
Fragments arranged by size:
447 310 38 24
Bsp1286I G_dGCh'C
Cuts at: 0 38 485 509 815 819
Size: 38 447 24 306 4
Fragments arranged by size:
447 306 38 24 4
BspEI T'CCGG_A
Cuts at: 0 462 819
Size: 462 357
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 278 819
Size: 278 541
BstAPI GCAn_nnn'nTGC
Cuts at: 0 42 819
Size: 42 777
BstXI CCAn_nnnn'nTGG
Cuts at: 0 123 155 258 438 819
Size: 123 32 103 180 381
Fragments arranged by size:
381 180 123 103 32
Bsu36I CC'TnA_GG
Cuts at: 0 11 819
Size: 11 808
BtgI C'CryG_G
Cuts at: 0 122 819
Size: 122 697
BtsI GCAGTG_nn'
Cuts at: 0 344 819
Size: 344 475
DrdI GACnn_nn'nnGTC
Cuts at: 0 786 819
Size: 786 33
EaeI y'GGCC_r
Cuts at: 0 49 746 819
Size: 49 697 73
Fragments arranged by size:
697 73 49
EarI CTCTTCn'nnn_
Cuts at: 0 52 321 514 819
Size: 52 269 193 305
Fragments arranged by size:
305 269 193 52
EcoICRI GAG'CTC
Cuts at: 0 507 819
Size: 507 312
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 112 217 247 415 471 568 733
Size: 52 60 105 30 168 56 97 165
Cuts at: 733 819
Size: 86
Fragments arranged by size:
168 165 105 97 86 60 56 52
30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 397 715 819
Size: 397 318 104
Fragments arranged by size:
397 318 104
EcoO109I rG'GnC_Cy
Cuts at: 0 15 543 812 819
Size: 15 528 269 7
Fragments arranged by size:
528 269 15 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 600 819
Size: 600 219
HaeII r_GCGC'y
Cuts at: 0 675 819
Size: 675 144
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 169 201 402 434 819
Size: 169 32 201 32 385
Fragments arranged by size:
385 201 169 32 32
KasI G'GCGC_C
Cuts at: 0 671 819
Size: 671 148
MfeI C'AATT_G
Cuts at: 0 432 819
Size: 432 387
MscI TGG'CCA
Cuts at: 0 51 819
Size: 51 768
NarI GG'CG_CC
Cuts at: 0 672 819
Size: 672 147
NcoI C'CATG_G
Cuts at: 0 122 819
Size: 122 697
NdeI CA'TA_TG
Cuts at: 0 174 819
Size: 174 645
NspI r_CATG'y
Cuts at: 0 9 819
Size: 9 810
PflMI CCAn_nnn'nTGG
Cuts at: 0 178 819
Size: 178 641
PfoI T'CCnGG_A
Cuts at: 0 710 819
Size: 710 109
PpuMI rG'GwC_Cy
Cuts at: 0 15 543 819
Size: 15 528 276
Fragments arranged by size:
528 276 15
PspOMI G'GGCC_C
Cuts at: 0 811 819
Size: 811 8
SacI G_AGCT'C
Cuts at: 0 509 819
Size: 509 310
SapI GCTCTTCn'nnn_
Cuts at: 0 321 514 819
Size: 321 193 305
Fragments arranged by size:
321 305 193
ScaI AGT'ACT
Cuts at: 0 616 819
Size: 616 203
SexAI A'CCwGG_T
Cuts at: 0 476 819
Size: 476 343
SfcI C'TryA_G
Cuts at: 0 281 819
Size: 281 538
SfoI GGC'GCC
Cuts at: 0 673 819
Size: 673 146
SmlI C'TyrA_G
Cuts at: 0 19 819
Size: 19 800
StyI C'CwwG_G
Cuts at: 0 46 122 819
Size: 46 76 697
Fragments arranged by size:
697 76 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 165 268 819
Size: 165 103 551
Fragments arranged by size:
551 165 103
TatI w'GTAC_w
Cuts at: 0 200 614 819
Size: 200 414 205
Fragments arranged by size:
414 205 200
Enzymes that do cut:
AcuI AhdI AlfI ApaI BanI BanII BbeI BbvCI
BcgI BclI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BseRI BseYI BsiHKAI Bsp1286I
BspEI BspMI BstAPI BstXI Bsu36I BtgI BtsI DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I KasI MfeI MscI NarI NcoI NdeI NspI
PflMI PfoI PpuMI PspOMI SacI SapI ScaI SexAI
SfcI SfoI SmlI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbsI BciVI BfrBI
BglI BglII BlpI BmgBI BmtI BsaAI BsaXI BsgI
BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I BtgZI
ClaI CspCI DraI DraIII EagI EciI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MluI
MmeI MslI MspA1I NaeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI