Restriction map for
pFN21AB9101
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflIII AgeI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BanI BanII BbeI
BbrPI BbvCI BciVI BclI BcnI
BglI BglII BlnI BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DrdI DsaI
EagI EclXI Eco47III Eco52I EcoO65I
EcoT14I FbaI FseI FspI HaeII
HapII HgaI Hin1I HpaII KasI
KpnI KspI MluI MroI MspI
MvnI NaeI NarI NciI NcoI
NgoMIV NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI StyI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AclI 1869 AACGTT AA^CGTT
AflII 2196 CTTAAG C^TTAAG
AspI 1184 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 839 CTCGAG C^[CT]CG[GA]G
BamHI 1413 GGATCC G^GATCC
BcgI 831 GCAGTATTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2196 CTTAAG C^TTAAG
BlpI 2747 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2747 GCTCAGC GC^T[ACGT]AGC
BseRI 653 CTCCTC GAGGAG(10/8)
BsoBI 839 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2954 TGTACA T^GTACA
BsrGI 2954 TGTACA T^GTACA
CelII 2747 GCTCAGC GC^T[ACGT]AGC
DraIII 1904 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2080 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1435 GATATC GAT^ATC
EcoT22I 76 ATGCAT ATGCA^T
FauI 2850 CCCGC CCCGC(4/6)
HpaI 1962 GTTAAC GTT^AAC
MfeI 1504 CAATTG C^AATTG
MunI 1504 CAATTG C^AATTG
NsiI 76 ATGCAT ATGCA^T
PaeR7I 839 CTCGAG C^TCGAG
PflFI 1184 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2506 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1869 AACGTT AA^CGTT
SphI 230 GCATGC GCATG^C
SspBI 2954 TGTACA T^GTACA
SspI 1247 AATATT AAT^ATT
SwaI 2724 ATTTAAAT ATTT^AAAT
Tth111I 1184 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 839 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AhdI 112 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2126 GACGTACTGTC
AocI 1555 CCTCAGG CC^T[ACGT]AGG
2422 CCTCAGG
AspEI 112 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2126 GACGTACTGTC
BbsI 2395 GAAGAC GAAGAC(2/6)
264 GTCTTC
BpmI 1646 CTCCAG CTGGAG(16/14)
2137 CTCCAG
BpuAI 2395 GAAGAC GAAGAC(2/6)
264 GTCTTC
BsaBI 598 GATACGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1255 GATAACAATC
BsaI 1944 GAGACC GGTCTC(1/5)
1688 GGTCTC
BsmI 279 GAATGC GAATGC(1/-1)
1913 GAATGC
BspMI 2376 ACCTGC ACCTGC(4/8)
1078 GCAGGT
BstF5I 1922 CATCC GGATG(2/0)
930 GGATG
BstXI 969 CCATCTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1925 CCATGTCAGTGG
BstYI 223 GGATCT [GA]^GATC[CT]
1413 GGATCC
Bsu36I 1555 CCTCAGG CC^T[ACGT]AGG
2422 CCTCAGG
CfoI 2372 GCGC GCG^C
3413 GCGC
Eam1105I 112 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2126 GACGTACTGTC
Eco81I 1555 CCTCAGG CC^T[ACGT]AGG
2422 CCTCAGG
EcoRI 1464 GAATTC G^AATTC
1731 GAATTC
FokI 1922 CATCC GGATG(9/13)
930 GGATG
HhaI 2372 GCGC GCG^C
3413 GCGC
HinP1I 2372 GCGC G^CGC
3413 GCGC
MamI 598 GATACGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1255 GATAACAATC
MflI 223 GGATCT [GA]^GATC[CT]
1413 GGATCC
MspA1I 1957 CAGCTG C[AC]G^C[GT]G
2812 CAGCTG
NdeI 1742 CATATG CA^TATG
3382 CATATG
NheI 345 GCTAGC G^CTAGC
1012 GCTAGC
NspI 230 GCATGC [GA]CATG^[CT]
3209 ACATGC
PvuII 1957 CAGCTG CAG^CTG
2812 CAGCTG
XbaI 2538 TCTAGA T^CTAGA
3067 TCTAGA
XcmI 924 CCAGAAGGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2972 CCAGCAGTTGCATGG
XhoII 223 GGATCT [GA]^GATC[CT]
1413 GGATCC
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI AspHI
AvaII BalI BbvI BfaI BmyI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII Fnu4HI HaeIII HincII
HindII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
RcaI RsaI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I